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Journal : SIMBIOSIS

IDENTIFICATION OF ‘TONGKOL’ FISH IN KEDONGANAN FISH MARKET, KUTA, BADUNG DISTRICT, BALI Aziz, Alifya Ibnu; Pharmawati, Made; Watiniasih, Ni Luh
SIMBIOSIS Vol 11 No 2 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i02.p06

Abstract

‘Tongkol’ fishes are species in the family Scombridae. There are many types of ‘tongkol’ fishes, namely longtail tuna, eastern little tuna, frigate tuna and bullet tuna. Morphologically, ‘tongkol’ species are similar to one another. This can result in errors in recording the production of ‘tongkol’ species. The purpose of this study was to identify ‘tongkol’ species collected from Kedonganan Market, Badung, Bali using the COI (Cytochrome C Oxidase Subunit I) gene. Two species of ‘tongkol’ fish were purchased from Kedonganan Market, Badung, Bali. DNA isolation was carried out from fish fins using chelex, followed by amplification of the COI gene segment. The PCR products were then electrophoresed and sequenced. DNA sequences were matched to the database using the Basic Local Alignment Search Tool (BLAST) found in NCBI. Extraction yielded DNA with a concentration of 5.91 ng/ml with a ratio of A260/A280 = 1.3 for sample 1, while for sample 2 the concentration of DNA was 6.27 ng/ml with a ratio of A260/A280 = 1.33. The resulting PCR product is about 700bp in size. The results of the COI gene sequences had a gene size length of 682 bp for both fish species. BLAST analysis yielded a percent identity of 99.84-100% with eastern little tuna (Euthynnus affinis) and bullet tuna (Auxis rochei).
Co-Authors Abdi Jana Guna Agung Mahayoni Aksari Akbar Akbar Aksari, Agung Mahayoni Alfi Hermawati Waskita Sari Anak Agung Gde Raka Dalem Ari Isnen Sobari Arie Setiadi Moerwanto Arliza Cynthia Razali Ayu Putu Wiweka Krisna Dewi Ayu Saka Laksmita W Ayu Wulan Trisna Aziz, Alifya Ibnu Beny Mustofa Chandra Yowani Dahlia Sandewi Deny Suhermawan Yusup Dewa Adi Putra Dewa Ayu Angga Pebriani Dhira Alfiah R. Pettalolo Dwikasari, Ida Ayu Indira ENIEK KRISWIYANTI Eyang Sriyani Toineno Fathur Rochman Felicia Augustine Florentina Gultom FX. Sudaryanto Husnayaen Husnayaen I Gede Hendrawan I Gusti Agung Ayu Suartini I Gusti Ayu Putu Intan Udayani I Gusti Ngurah Kade Mahardika I K. Putra Juliantara I Kadek Teguh Indra Dewantara I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I Komang Andika Putra I Made Gatot Wija Damara I Made Kardena I Made Merdana I Nengah Nuyana I Nyoman Suarsana I Nyoman Suartha I Putu Candra Noviarta I Putu Gede Bayu Ade Wianta I PUTU NARKA EKA PRATAMA I W BUDIARSA SUYASA I Wayan Arthana I Wayan Arthana I Wayan Kasa I Wayan Suardana I. G. A. PRADANA PUTRA Ida Bagus Made Suaskara Iwan Harjono Utama Juan Aldo Jaya Pradipta Sitepu Julian Tambunan Juniartini, Nyoman Sweet Kadek Bayu Prabha Mahesswara Kadek Wiwik Widaswari Kartika, I Wayan Darya Komang Gede Wahyu Saputra Kompyang Bagus Suyoga LUH Puru ESWARYANTI KUSUMA YUNI M Windhu Made Ayu Pratiwi MADE PHARMAWATI Mahendra Duwi Astutik Mufadilatur Rohmah Ni Gusti Made Yuliari Ni Kadek Febri Yanti Ni Ketut Suwiti Ni Luh Putu Ria Puspitha ni luh wayan hanny prabandari Ni Made Ernawati, Ni Made Ni Made Sekarmini Ni Made Suartini Ni Pande Putu Suji Dian Antari Ni Putu Adriani Astiti Ni Putu Dian Pertiwi Ni Putu Kiki Mahayuni Ni Putu Putri Wijayanti Ni Putu Sintha Maharani Ni Putu Sri Ayuni Ni Wayan Sudatri Niko Susanto Putra Odjastira Vidyastari Pramurdya, Yesika Nanda Putu Ade Hinduari Putra Putu Angga Wiradana Putu Ayu Parwati Putu Ayu Wiwin Vitrayanthi Putu Frissa Anasthasya Widhiandari Putu Laksmi Candra Dewi Resti Ayu NengTias Retno Kawuri Reza Yunita Sari Romlah Setiawati Roni Adi Samba Suprabadevi Ayumayasari Saraswati Suprabadevi Ayumayasari Saraswati WIDIASTUTI - Widiastuti Widiastuti Yesi Breanita Yulia Kartika