Article Per Year (5 Year)
p-Index From 2021 - 2026
1.665
P-Index
This Author published in this journals
All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Jurnal Bios Logos SIMBIOSIS Biosaintifika: Journal of Biology & Biology Education Jurnal Widya Biologi
Ni Nyoman Wirasiti
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

Published : 21 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 21 Documents
Search

 1   2   3 
Optimasi PCR dengan Penanda Daerah D-loop DNA Mitokondria untuk Metode Tes DNA Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 12 No 2 (2022): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2022.v12.i02.p03

Abstract

DNA testing is a molecular personal identification that can distinguish between individuals. One of the DNA markers used for PCR tests is mitochondrial DNA markers. Mitochondrial DNA is reported to have a polymorphic locus in the mitochondrial D-loop region. This study aims to determine the appropriate method to amplify mitochondrial DNA in the D-loop region for the DNA testing. A sample of 65 people was taken by purposive sampling method. Samples were extracted with phenol-chloroform and PCR with 2 types of PCR formulas, is formulas A and formulas B and the annealing temperatures used were 48oC, 52oC dan 56oC. The results showed that the optimal primary concentration for successful amplification was a Formulation B with primer concentration of 0,4 µM and condition variation II with annealing temperature of 52oC. A total of 62 Balinese people were successfully amplified with an amplicon length of 860 bp. Samples that are not successfully amplified are affected by the number of collected epithelial cells, the success of the DNA extraction process and null allele. So, from these results it can be concluded that the optimal conditions of DNA marker with a primer concentration of 0,4 µM and annealing temperature conditions of 52oC succeeded in amplifying the D-loop region of mitochondrial DNA.
Co-Authors A. A. G. R. Dalem A.A.S. A. Sukmaningsih AGUNG EKO CAHYA KUSUMA PUTRA Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andi Ratu Bulqis I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I Ketut Junitha I KETUT MUKSIN I W. Suarna I.G.A.S. Wahyuni Ida Bagus Bajing Agastya IRIANI SETYAWATI Kezia Artanauli Purba LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Wrasiati MADE PHARMAWATI N.G.A.M. Ermayanti Ni Made Rai Suarni NI NYOMAN DARSINI Ni Putu Putri Wulandari Ni Putu Sukma Widyantari Ni Wayan Sudatri Retno Kawuri Sally Nabila Rizal Septiasari, Ni Putu Senshi Tyara Tyara Yoseph Raymond Irawan