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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Journal of Tourism INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Master Pariwisata (JUMPA) Jurnal Biologi Tropis International Journal of Social Service and Research Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Nandur ARIMA : Jurnal Sosial Dan Humaniora Biosaintifika: Journal of Biology & Biology Education
I GEDE PUTU WIRAWAN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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ISOLATION AND ABILITY TEST OF PHENOL-DEGRADING BACTERIA FROM THE LIQUID WASTE IN BHAYANGKARA HOSPITAL, DENPASAR CITY, BALI, INDONESIA Shara Yulita Harianja; I Gede Putu Wirawan; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v09.i01.p06

Abstract

Phenol is an organic compound that is toxic to humans and the environment. This compound was contained in the hospital liquid waste and petrochemical-based industrial sectors. This study aims to obtain hydrocarbonoclastic bacterial isolates that act as biodegradation agent in waste handling. Samples obtained from the biofilter tank of liquid waste treatment Bhayangkara Hospital Denpasar,Bali, Indonesia then carried out isolation and inoculation of bacteria in selective media NA (Nutrient Agar) containing Phenol. The bacteria obtained was performed the viability test on the media with various Phenol concentration levels, obtained three isolates of bacteria capable of growing to the concentration Phenol 500 mg / L, i.e. isolates IB2, IB5, and IB10. Therefore, it was performed the degradation ability and rate test on those three isolates. After 72 hours of incubation periods, the change of Phenol concentrations was analyzed using the folinciocalteau method and examined using the spectrophotometer with 660 nm of wavelength. Based on the analysis of final phenol content, the IB2 isolate has the highest degradation capability in the concentration of 600 mg/L (153,4 mg) with a degradation rate of 2,13 mg/h. IB5 isolates have the highest degradation capability in the concentrations of 700 mg/L (109,45 mg) with a degradation rate of 1,52 mg/h. Meanwhile, IB10 isolates show the lowest ability and degradation rate in both concentrations. Through morphological identification and Gram stain, three isolates belong to the Gram-negative rod-shaped bacteria, which has similarities with the Pseudomonas genus.
INCREASING LOCAL FRUITS COMPETITIVENESS IN ENTERING THE TOURISM MARKET IN BALI I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih; Wayan Adiartayasa; I Nyoman Wijaya; I Putu Anom
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The study focused on improving the fruit quality supported by handling of the cultivation, harvest and post-­??harve st handling such as fruit packing, transportation, storage, and others, as well as marketing strategiesand regulations that are ne eded locally, so local Bali fruits can compete better in the Bali tourism market.The purposes of this study were to find some problems face by the local fruits to e nter the tourism marke t inBali. The local fruits studied were citrus, salacca, mangoes, mangosteen, banana, and papaya. This study wasthrough multiple surveys such as, inte rviews with farme rs, whole salers, fruit suppliers, and the manage rsof the hote ls and restaurants. The results of this study found some local fruits have e nte red the tourismmarket in Bali, in addition, local people in Bali te nd to consume the imported fruits for some culturalceremony, events and others.The results of this study showthat the main problems in the local fruits suchas citrus and banana were harvesting, packaging, storage, and transportation. The laboratory studies foundthat some microbes and inse cts infe cted local fruits due to the lackof handling such as packaging, storage,and transportation. The findings of this study was local fruits such as orange s and bananas face manyobstacles ranging from harvest, post-­??harvest management, transportation, and the marketing strategies.Many problem has found on bananas such as damaged on storage, as well as citrus fruits. Improvements tothe appearance of the fruit has been tried to do by the application of emulsion of he rbal oils, oleic acid, wax,twee n, and the results showe d the fruits still in a fre sh condition afte r four weeks of treatme nt while theuntre ated fruits were rotten due tothe infe ction of microbes. The blackspots of insects ormicrobes infectionson the treated fruits can be eliminated as well. Some inse cts and microbial attacks were found that leads tofruit rot which can essentially be removed by tre atment with antimicrobial tre atment. This research tries tochart the effects on the competitiveness of local fruits, such as continuity of production, standardization andregulation which could be developed at the local level in the village community.
HAMA DAN PENYAKIT PADA TANAMAN JERUK SERTA PENGENDALIANNYA I N. Wijaya; W. Adiartayasa; I G.P. Wirawan; M. Sritamin; M. Puspawati; I M. Sudarma
Buletin Udayana Mengabdi Vol 16 No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Sebagian besar penduduk Desa Katung, Kecamatan Kintamani, Kabupaten Bangli sebagai petani jeruk yang tergabung dalam kelompok Subak Abian Kerta Winangun. Pertanaman jeruk di daerah ini sangat berhasil, sehingga pendapatannya meningkat. Namun belakangan, petani resah karena tanaman jeruknya terserang hama dan penyakit terutama penyakit CVPD (Citrus Vein Phloem Degeneration) dan hama lalat buah. Oleh karena itu perlu diadakan penyuluhan dan pelatihan tentang pengelolaan hama dan penyakit pada tanaman jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama dan penyakit serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan hama dan penyakit, cara penularan penyakit serta cara pengendaliannya. Metode yang digunakan dalam pelatihan ini adalah penyampaian materi di ruangan dan praktek di perkebunan jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama dan penyakit serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan hama dan penyakit, cara penularan penyakit serta cara pengendaliannya. Pelatihan ini dipandu oleh 5 (lima) orang pelatih yang berasal dari Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana. Pelatihan diikuti diikuti oleh 50 orang peserta. Penceramah memaparkan materinya dengan menggunakan alat peraga berupa power point dan menggunakan spesimen berupa gejala serangan hama dan penyakit. Kemudian dilanjutkan dengan praktek lapangan selama 60 menit. Masing-masing peserta diberi tugas untuk mencari tanaman jeruk yang terserang hama dan penyakit serta penyebabnya. Dalam pelaksanaan pelatihan tersebut, petani sangat antusias untuk mengikuti penyuluhan. Hal ini terbukti dari banyaknya pertanyaan-pertanyaan yang diajukan. Untuk mengetahui keberhasilan kegiatan ini, maka dilaksanakan evaluasi dengan pengisian kuisioner sebelum dan sesudah pelatihan.
PELATIHAN BUDIDAYA TANAMAN JERUK BEBAS PENYAKIT CVPD DI DESA KERTA GIANYAR W. Adiartayasa; I.N. Wijaya; I. G.P. Wirawan; N.N. Darmiati; I K. Siadi
Buletin Udayana Mengabdi Vol 16 No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Penyakit CVPD (citrus vein phloem degeneration) merupakan penyakit penting pada tanaman jeruk. Penyakit menyebar melalui bibit tanaman jeruk yang telah terinfeksi CVPD dan ditularkan melalui serangga vektor D. citri. Tanaman jeruk yang terserang CVPD memperlihatkan gejala daun menguning atau klorosis, warna tulang daun tetap hijau, ukuran daun menjadi kecil dan daun menjadi tebal dan kaku. Hasil pengamatan tanaman secara morfologi di Kecamatan Kintamani didapatkan 10 jenis tanaman jeruk yaitu jeruk Siam, Selayar, Besakih, Tejakula, Manis, Nipis, Purut, Lemo, dan jeruk Bali. Gejala penyakit CVPD pada masing-masing jenis tanaman jeruk mempunyai gejala klorosis bervariasi. Pelatihan ini bertujuan untuk menambah pengetahuan budidaya tanaman jeruk bebas penyakit CVPD. Pelatihan ini telah dilaksanakan di Kantor Desa Kerta, pada hari Rabu tanggal 10 Agustus 2016. Kegiatan ini diikuti oleh 20 orang petani dan lima orang dosen Fakultas Pertanian Unud. Hasil evaluasi yang didapatkan 15 orang menguasi 90 persen materi, dan 5 orang menguasai 60 persen materi. Petani mengharapkan adanya pelatihan dan pendampingan yang berkelanjutan mengenai budidaya tanaman jeruk.
The Image of Bali Tourism in Social Networking Media Ni Gusti Ayu Dewi Paramita Arisandi; I Gede Putu Wirawan; Linawati Linawati
E-Journal of Tourism Volume 1 Number 2 (September 2014)
Publisher : Centre of Excellence in Tourism Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.381 KB) | DOI: 10.24922/eot.v1i2.19425

Abstract

Tourism is growing very rapidly with the development of technology, especially information technology .Through information technology people can easily get access to information about various destinations and tourist attractions as well as hotel by online media communication. Therefore, this study was trying to use social media such as facebook and twitter, where both social media were widely used by companies engaged in tourism in the world as well as in Bali. Internet consists of several websites, but the most effective one to conduct the research on tourism such as webseries 2.0 or commonly called as social networking media. Social medias - Facebook and Twitter - both were wildly used by the companies running in the sector of tourism in Bali, such as Hotels, Villas, Restaurants, Spas, Travel Agents, Airlines, and Tourist Attraction Managements, to advertised their products and services in their respective field. Through those social media, tourists will be able to access the comments or reviews about the companies. The problem raised in this research is how Bali’s tourism data in social networking media were used and considered, and how was the image of Bali’s tourism in their point of viewbased on the research. The method used here was descriptive qualitative method which describes this phenomenon descriptively by analyzing the obtained comments on social networking media such as facebook and twitter, and then classified in the form of comments positive, negative, and unidentified comment. Afterwards comments were re-analyzed through 4A Approach which consist of Attraction, amenities, Ancillary, and Accessibility. Data which has been obtained re-analyzed via the data obtained from the homepage of (TripAdvisor and Agoda.com). In addition, to strengthen the research data obtained from the homepage of TripAdvisor and Agoda.com , then it has been elaborated on the point rating system which indicatedthat the company were registered in both of those social networking media such as Hotel, Villa, SPA, restaurants, travel agents, airlines, media, tourist attraction management has a good rating result, and it has represented that the image of Bali tourism has a good rank in the eyes of local and international tourists. This data was also strengthened by the existence of empirical studies where there were the data from a hundred tourists came to Bali both locally and internationally has filled out the questionnaire about Bali in order to obtain maximum results on the image of Bali tourism. The results showed that, social networking media were effectively used in this study. Many companies in Bali used facebook and twitter. In addition, Bali Tourism image placed in positive rank on both of social networking sites, and it was gained by the results of questionnaires spread towards the visitor.
THE DEGRADATION OF DIESEL OIL BY CONSORTIUM OF BACTERIA IN SHAKEFLASK CULTURES Ciawi -; Safitri R.; Suniti N.W.; Sahanggamu Y. P.; Dharmawibawa I.D.; Wirawan I.G.P
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Diesel fuel is widely used in manufacturing and transportation industries. Its production, transportation, use and disposal have the risk to contaminate the environment. In the other hand contaminated site usually can produce some microorganisms, through natural selection, that can utilize the hydrocarbons as source of energy and food thus clean the environment itself. For bioremediation purposes, 14 isolates of diesel oil degrading bacteria from Bandung, Jakarta, and Bali has been employed for diesel oil degradation. These 14 isolates, together with other variables, e.g. pH, Pb, sucrose and urea, were plotted in 23x24 matrixes of Plackett and Burman statistical method. Fermentation was carried out in batch cultures for 14 days. The results showed that 9 isolates and the availability of sucrose and higher initial pH have a positive/stimulatory effect on diesel oil biodegradation, 5 of which were very significant (99.99%), whereas 1 isolate has inhibitory effect (-98%). The results also revealed that 8 isolates, higher initial pH, and availability of urea have stimulatory effect on culture growth (more than 90% significant). The present of Pb in medium inhibit both growth and the degradation of diesel oil, although the effect was not significant (less than 60%).
Aktivitas In Vitro Anti Jamur Ekstrak Bulung Sangu Gracilaria sp. terhadap Jamur Patogen Fusarium solani (Mart) Sacc. Cabai Rawit ANDRIANI ANDRIANI; I GEDE PUTU WIRAWAN; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Antifungal Activity of Gracilaria sp. against Pathogenic Fungi Fusarium solani (Mart) Sacc. Cayenne pepper The use of chemical pesticides as a control for cayenne pepper fusarium wilt caused by the Fusarium solani continuously for a long time can have a negative impact on the ecosystem, and toxic for humans. Seaweed Gracilaria sp. as a marine commodity that is abundant in Indonesia, especially in the Bali region, it contains bioactive compounds that have the potential to be developed as an antifungal. This study was aimed to determine the effectiveness of Gracilaria sp. in inhibiting the growth of Fusarium solani (Mart) Sacc. This research was a single factor experiment which was compiled based on a completely randomized design (CRD) consisting of six treatments of the concentration of Gracilaria sp. and each treatment was repeated 3 times. The treatment was given various concentrations of Gracilaria sp. in the fungus F. solani, the concentration is 0%; 0.5%; 1%; 1.5%; 2%; and 2.5%. Treatment was positive control (ketoconazole) and negative control (Tween 80). The results of this study indicate that the extract of Gracilaria sp. has antifungal activity that is fungistatic with weak inhibition against the fungus F. solani. MIC test results show that the minimum extract that can inhibit the growth of Fusarium solani is a concentration of 0.5%. MIC test results show that the minimum extract that can inhibit the growth of F. solani is concentration of 0.5% with an average diameter of 4 mm with a weak category. The highest inhibition power of Gracilaria sp. to F. solani in this study was 2.5% with inhibition of colony growth of 79.3%. The percentage of spore growth inhibition was 67.49%. The concentrations studied showed that the higher the extract concentration, the greater the inhibition power of Gracilaria sp. against F. solani.
Kultur In vitro Tanaman Jeruk Pamelo (Citrus maxima Merr.) Menggunakan explant Biji Serta Deteksi Keberadaan Bakteri Liberobacter asiaticus pada Kalus yang Dihasilkan PUTU BAMBANG IKA WINJALISTA; I GEDE PUTU WIRAWAN; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

In vitro culture of citrus plant Pamelo (Citrus maxima Merr.) Using Explant seeds as well as the detection of the presence of Bacteria Liberobacter asiaticus at Shoots are produced This research was begun with doing sample approachment in Kapal Village, Mengwi Sub-District, Badung regency and Sumerta Klod Village, East Denpasar Sub-District, Denpasar City and the culture in vitro was done in Implementing Technical Unit (UPT) Sumberdaya Genetika dan Biologi Molekuler Laboratory since October 2018 until January 2019. This research aim to recognize the response of pamelo orange's seed explant rather it will grow in based MS media which has been richen and got pamelo orange seed that is free from the infection of liberobcter asiaticus. The approachment of sample was based on the visualization of the plant, the plant which shows any indication of CVPD disease next will be known as 1st Sample and 2nd sample would be the plant which shows no indication of cvpd disease. The seed of each sample be cultured with MS media which has been richen, was maintained for 8 weeks after it's planted (mst). By the results of this research, it concludes that there's seed explant from both samples which grows without showing any indication of cvpd disease, but the result of DNA amplification from 1st sample that has been cultured in vitro after got UV transluminator visualization shows the existence of 1160 bp DNA ribbon which is belong to Liberobcter asiaticus. This shows that the sample has been infected by Liberobacter asiaticus, whereas can't be found in 2nd sample.
Deteksi Penyakit Tanaman Cabai Rawit (Capsicum frutescens L.) yang Tumbuh Disekitar Tanaman Jeruk Bergejala Citrus Vein Phloem Degeneration (CVPD) Menggunakan Teknik Polymerase Chain Reaction (PCR) NI KADEK DWI PASARI; I GEDE PUTU WIRAWAN; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Detection of Chilli plant (Capsicum frutescens L.) which Grow in the Area of Citrus Cultivation that had symptoms of Citrus Vein Phloem Degeneration (CVPD) Using Polymerase Chain Reaction (PCR) Technique Liberibacter bacteria live and thrive inside the phloem tissue CVPD affected plants, the bacteria exhibit a progressive degeneration of the phloem tissue that inhibit phloem nutrient transport through the entire plan.. As the attack of Liberibacterasiaticus cause Citrus Vein Phloem Degeneration (CVPD) disease in citrus plant, it is necessary to do research on the chilli plant around citrus plant area to know whether bacteria of Liberibacter also attack the chilli plant by using PCR.The results of this study indicate that the chilli plants with CVPD symptoms which are suspected to be caused by the bacterium Liberibacter by usingLiberibacter asiaticus primer and Liberibacter solanaceae which is them specific primer of 16S rDNA are not proven. The result showed chilli plants with similar symptom to CVPD in Mangguh village, Kintamani sub-district were not to be caused by Liberibacter asiaticus and Liberibactersolanaceae.
Isolasi dan Identifikasi Bakteri Selulolitik pada Sampah Organik di TPA Suwung Denpasar FADEL ALKAHFI; WAYAN ADIARTAYASA; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Isolation and Identification of Cellulolytic Bacteria in Organic Waste at TPA Suwung Denpasar Organic waste contains a lot of lignocellulosic material which can be degraded by cellulases. The degradation of cellulose requires three types of enzymes produced by microorganisms. Cellulolytic bacteria are one of the microorganisms involved in the decomposition process and produce cellulase enzymes which can degrade organic matter. This study aims to isolate and test the ability of cellulolytic bacteria in the organic waste of TPA Suwung Denpasar by isolating morphological identification, and quantitative cellulase test. The results of this study found twenty-seven (27) bacterial isolates that were able to grow on CMC media and were classified based on colony morphology and obtained 3 different isolate groups. The isolates that had been grouped were coded A, B, and C. The colony of the isolated isolates appeared to be round and wrinkled. Some surfaces are convex, raised, and hilly and are clear, yellows in color, and white. Isolates A and C were gram positive, while isolate B was gram negative. Based on the calculation results of the Cellulolytic Index value, it was found that isolate B had the highest IS and was in the high category.
Co-Authors - Afrizal ANDRIANI ANDRIANI Antony Lay Aulanni ‘am Aulanni ‘am Bambang Sugiharto BAYU ARYA BAGASKARA Ciawi - DARWIN SILALAHI DEWA AYU RATIH Dewi, Ni Kadek Emi Sintha Dharmawibawa I.D. DINA RAHMAWATI Evy Prima FADEL ALKAHFI Gayatri, Anak Agung Sagung Intan Gresia Eldina Haryadi, Celvin Hengky Surya Bhuana I Gede Ketut Susrama I Gusti Ayu Diah Yuniti I Ketut Siadi I Ketut Suada I Ketut Suada I M. Sudarma I Made Parama Nanda Mahatya I N. WIJAYA I NYOMAN MANTIK ASTAWA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I NYOMAN WIJAYA I Putu Agus Hendra Wibawa I Putu Anom I Putu Sudiarta I Putu Wahyu Sanjaya I WAYAN PRASTITA DIASTAMA I WAYAN SUAMBA I.N. Wijaya Ida Ayu Putri Darmawati K. Sri Marhaeni Julyasih K. Suata Kalimutu, Putu Krisnawan Kayo Gouya Ketut Sri Marhaeni Julyasih Ketut Srie Marhaeni Julyasih Liliek Sulistyowati Linawati Linawati Luh Putu Wrasiati M. Puspawati Made Adnyana Made Heny Urmila Dewi MADE SRITAMIN Merlyna Rosalia Motoyuki Suzuki Munthe, Lambok Jenny Sagita MUSLIM MUSLIM NADIAH SILITONGA Nevy Widya Pangestika Ni Gusti Ayu Dewi Paramita Arisandi NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI MADE ADHYA NIDIDHYA SANI Ni Made Ayuratih Utami Ni Made Regina Prasetya Putri Ni Made Suaniti NI NENGAH DARMIATI NI WAYAN ARMINI Ningsih, Gusti Ketut Asti Dea Nobuyuki Kohno Novayolla, Alsha Nurftatmelia Nyoman Agus Bagiada Parwata, I Made Oka Pranata, Kadek Dwipayana Pranatayana, Ida Bagus Gede PUTU BAMBANG IKA WINJALISTA PUTU SENA WIDIATMA Riezchy Riando Mamanua Rindang Dwiyani Rizki, Muhamad Farrel ROMAULI THERESIA NAINGGOLAN Romulia Trielva Apriliani Rusmita, Gst Ayu Putu Ika Yulia Safitri R. Sahanggamu Y. P. Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Setianingsih, Ni Luh Putu Putri Shara Yulita Harianja Shchegolkova, Nataliya Shin-ichi Sekiyama Simarmata, Jonatan Alexius Suniti N.W. TRISNA AGUNG PHABIOLA VANI SILVANA VEDERIS LEUNARDUS VICKY TANDYA WAYAN ADIARTAYASA WHISNU IDA BAGUS YOLANDA HASSIAN MANALU Yundari, Yundari