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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Master Pariwisata (JUMPA) agriTECH Jurnal Biologi Tropis International Journal of Social Service and Research Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Jurnal Pustaka Mitra : Pusat Akses Kajian Mengabdi Terhadap Masyarakat Nandur Biosaintifika: Journal of Biology & Biology Education
I GEDE PUTU WIRAWAN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Shchegolkova, Nataliya; Kalimutu, Putu Krisnawan; Wirawan, I Gede Putu; Suada, I Ketut
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p06

Abstract

Indonesia is the third largest cocoa bean producing country in the world after Ghana and the Ivory Coast with current Indonesian cocoa bean production of 600,000 tons per year. The quality of cocoa is still relatively low compared to the harvest potential that should be obtained, such as the Sulawesi III clone which provides a yield potential of 2-2.4 tons per hectare. This study aims to obtain indigenous bacteria from Meko village so that the role of each bacteria can be further investigated. The results of growing bacteria on LB media found 25 isolates which were observed to have quite varied morphology including round and irregular shapes, having rough and dull and slimy surfaces, opaque, cloudy and colored (yellow and reddish) and having flat and rising elevations. Most of the bacteria obtained were rod-shaped, namely 23 were gram negative and three were gram positive. The shape of the cell is dominated by the shape of the rod, about 19 are rod-shaped and the remaining six are round.
ANTIFUNGAL ACTIVITY OF SIAM CITRUS (Citrus nobilis L.) ESSENTIAL OIL AGAINTS Lasiodiplodia theobromae THE PATHOGEN OF BLENDOK DISEASE Pranata, Kadek Dwipayana; Wirawan, I Gede Putu; Wibawa, I Putu Agus Hendra; Suada, I Ketut; Wijaya, I Nyoman; Phabiola, Trisna Agung
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p08

Abstract

Siam citrus (C. nobilis L.) is a type of citrus that is most widely cultivated in Bangli Regency. The main disease that attacks citrus plants is blendok caused by the fungus L. theobromae. Based on research siam citrus peel contains essensial oils that have potential as antifungals. The aim of this study was to study chemical content of essensial oil from siam citrus peel and its activity as an antifungal againts L. theobromae. Essensial oil was destilled using hydro steam distillition and analyzed by means of GC-MS. Antifungal activiy testing was carried out using the agar-well diffusion method with concentrations of 1%, 10%, 25%, 50%, 75%, and 100%. Dithane M45 6 g/l and DMSO 10% were used as positive and negative controls. The results showed that the siam citrus peel from Kintamani, Bangli contains essensial oils with the main chemical components, i.e. D-limonene (57.26%), Beta-pinene (9.09%), and Beta-myrcene (4.03%) which has uses as an antifungal, additive, antitumor, asthma and allergy reliever, repellent, anti-inflammatory, antioxidant, anticancer, and antibacterial. Essential oil concentration of 25 to 100% can inhibit the growth of L. theobromae. The largest diameter of inhibition is shown at a concentration of 100% and the smallest diameter of inhibition is shown at a concentration of 25%. Keywords: C. nobilis L., essensial oils, GC-MS, L. Theobromae, antifungal
Formulation And Effectiveness Of Bulung Sangu (Gracilaria Gracilis) Scrub For Skin Moisturization And Brightening Simarmata, Jonatan Alexius; Wirawan, I Gede Putu; Pranatayana, Ida Bagus Gede
International Journal of Social Service and Research Vol. 5 No. 10 (2025): International Journal of Social Service and Research
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/ijssr.v5i10.1326

Abstract

Free radicals (UV rays). Therefore, the best solution is needed by using skincare products containing antioxidants. Antioxidants can neutralize free radicals from UV rays. The hallmark of a product attracts people to buy, try, or feel the benefits of skincare ingredients. This research is experimental to determine the effectiveness of ingredients from bulung sangu, which are formulated in the form of fine powder for skincare. The study used four scrub samples, namely bulung sangu (Formulation 1 dan 2), without bulung sangu (Formulation 3), and Bali Alus Seaweed (Formulation 4) which were used for 2 weeks by 30 panelists from the Faculty of Agriculture, Udayana University with hedonic tests for appearance, aroma, color, and texture of the scrub and tests the effectiveness of moisture and brightness to the skin. The results obtained, Formulation 4, the highest average value and total percentage of hedonic (78%, 79%, 81%, 83%) and Formulation 2, the lowest average value and total percentage of hedonic (64%, 56%, 63%, 61%). The results of the effectiveness test Formulation 1 have the highest value, namely skin moisture normal to slightly moist (38% to 46%) and tanned skin to bright white skin (15 to 9). Meanwhile, Formulation 4 from Bali Alus Seaweed, have the lowest value, namely skin moisture normal to slightly moist (41% to 44%) and the skin remains in the tanned skin (15). This is caused by several factors, gene experession, environment, activity and physical condition, and the effectiveness of the preparation of scrub product ingredients.
Morphological Identification of Balinese Caulerpa Seaweeds Dewi, Ni Kadek Emi Sintha; Wirawan, I Gede Putu; Suada, I Ketut
Jurnal Biologi Tropis Vol. 24 No. 2b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2b.7858

Abstract

Bulung boni and bulung anggur are species of Caulerpa seaweeds commonly found along the coastline of Serangan Island, Bali. Although these species have been widely utilized in various ways, formal species identification of these two macroalgae in Bali had not been conducted prior to this study. This research aims to characterize the morphological structures of bulung boni and bulung anggur through detailed morphological examination. Identification keys and previous studies were used to assist in the morphological identification process. The findings revealed that bulung boni shares morphological characteristics with Caulerpa cylindracea, while bulung anggur is morphologically comparable to Caulerpa macrophysa. These results provide valuable insights into the species composition of seaweeds in the region and contribute to a better understanding of local biodiversity.
Increased Attractiveness and Shelf Life of Siamese Tangerines (Citrus Nobilis L.) Using Various Palmitic, Stearic, and Oleic Acid Emulsions and Mixtures Setianingsih, Ni Luh Putu Putri; Suaniti, Ni Made; Wirawan, I Gede Putu
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v7i2.204

Abstract

Along with the growing number of people, the need for food is also growing. Citrus nobilis l is one of the many well-known fruits. Citrus nobilis L, both in its fresh and processed forms, is easily broken if it is not treated correctly. This study hopes to make things look better and save power simultaneously by coming up with different emulsions. Palmitic acid, stearic acid, oleic acid, and a mix make the slurry. Organoleptic study of Citrus nobilis L., vitamin C content, water content, and identification of compounds using Gas Chromatography-Mass Spectrometry (GC-MS). Evaluation of organoleptic analysis test done by hedonic test (colour, smell, texture, taste, and general acceptance) and test scoring (colour, texture). A combination of palmitic, stearic, and oleic acids in an emulsion will make tangerine fruit look better and last longer. How vitamin C and water are measured and analyzed affects different emulsions. When citrus fruits are treated with palmitic, stearic, and oleic acid solutions, the lowest quality drops to 14.4851 mg of vitamin C per 100 grammes and 91.8401% water. The total amount of volatile compounds in fruit orange conjoined DL-Limonene has many different areas of volatile compounds in each treatment emulsion.
DNA Barcoding Based on matK Gene and Phytochemistry Analyses of Local Balinese Kayu Tangi ( Lagerstroemia sp.) Suada, I Ketut; Wirawan, I Gede Putu; Ningsih, Gusti Ketut Asti Dea; Wijaya, I Nyoman; Munthe, Lambok Jenny Sagita; Gayatri, Anak Agung Sagung Intan; Sasadara, Maria Malida Vernandes; Parwata, I Made Oka
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.9265

Abstract

Kayu tangi (Lagerstroemia sp.) is one of the medicinal plants that is often used traditionally by Balinese people for treating insomnia, diabetes, and dysentery. This study aims to determine the phylogenetic trees and the active compounds in Lagerstroemia tomentosa extract and its antioxidant activities. The methods used in molecular identification are DNA isolation, PCR, and DNA sequencing. The DNA sample was BLASTed to see its homology with the sequences in GenBank. These sequences were then used for phylogenetic tree analysis. The phytochemical analysis was done using the GC-MS method and the antioxidant activity test used the DPPH (1,1-diphenyl-2-picrylhydrazyl) after being reacted with the tested extract.  The results of molecular identification showed that the samples used were closely related to Lagerstroemia tomentosa species (MW044208.1). The antioxidant activity test results showed an IC50 of 2.1 ugml-1 which is included in the very strong category. The phytochemical test results showed that the most dominant compounds contained in the plant stem bark were furfural (AUC 8.54%), beta-sitosterol (AUC 6.00%), and gamma-sitosterol (AUC 3.81%). Based on PubChem data, these compounds have antioxidant and antimicrobial properties as well as insecticide and herbicide. In conclusion, kayu tangi is very close to  Lagerstroemia tomentosa and it’s potentially used as a medicine with strong antioxidant activities.  
Bioactive Compounds and Antibacterial Activity of Red Beetroot (Beta vulgaris L.) Ethyl Acetate Extraction Pranatayana, Ida Bagus Gde; Tampubolon, Immanuel Anthonio Caesebo; Wirawan, I Gede Putu; Wijaya, I Nyoman; Suada, I Ketut; Khalimi, Khamdan; Yudha, I Kadek Wisma
agriTECH Vol 46, No 1 (2026)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.105476

Abstract

Red beetroot has been reported to possess various medicinal properties and antimicrobial activity. Therefore, this study aims to determine the compounds contained in the ethyl acetate extract of red beetroot pulp and evaluate its anti Escherichia coli effect. The extract was obtained by soaking the fruit in ethyl acetate, and then assessed for its components using GC-MS analysis. Antibacterial activity was tested using a disc diffusion assay. The results showed that the ethyl acetate extract of red beetroot pulp contained a total of 20 major compounds. These included ester of 1,3-benzenedicarboxylic acid and bis(2-ethylhexyl), 1,4-benzenedicarboxylic acid, and n-hexadecanoic acid. In the antibacterial test, the sample was highly effective against E. coli (IC 50 was 0,783 mg/mL). In addition, the diameter of the growth inhibition zone increased along with the extract concentration. These results suggest that the ethyl acetate extract of red beetroot pulp offers a natural antibacterial substitute to its synthetic equivalents.
Hilirisasi Produk Inovasi Pertanian Berbasis Rumput Laut melalui Kolaborasi Akademik dan Industri Pranatayana, Ida Bagus Gde; Wirawan, I Gede Putu; Suada, I Ketut; Yudha, I Kadek Wisma; Khalimi, Khamdan; Phabiola, Trisna Agung
Jurnal Pustaka Mitra (Pusat Akses Kajian Mengabdi Terhadap Masyarakat) Vol 6 No 3 (2026): Jurnal Pustaka Mitra (Pusat Akses Kajian Mengabdi Terhadap Masyarakat)
Publisher : Pustaka Galeri Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55382/jurnalpustakamitra.v6i3.1394

Abstract

Kegiatan pengabdian kepada masyarakat ini dilaksanakan di PT. Alga Nusa Sejahtera, Nusa Lembongan, Bali, dengan tujuan memperkuat hilirisasi inovasi pertanian berbasis rumput laut melalui kolaborasi antara akademisi, industri, dan lembaga pemerintah. Kegiatan dilakukan secara kualitatif-partisipatif melalui observasi lapangan, penyampaian materi, diskusi, pendampingan teknis, serta visitasi laboratorium bersama Balai Besar POM Denpasar. Kegiatan diikuti oleh 18 peserta, dengan 12 peserta dari pihak mitra sebagai sasaran utama evaluasi. Hasil evaluasi menunjukkan adanya peningkatan pemahaman peserta dari skor rata-rata pre-test 2,02 menjadi 3,47 pada post-test, atau meningkat sebesar 71,8%. Peningkatan tertinggi terjadi pada aspek pemahaman GMP/CPPOB sebesar 85,7%, yang menunjukkan pentingnya pendampingan lanjutan pada aspek standar produksi dan keamanan pangan. Selain meningkatkan pemahaman mitra, kegiatan ini juga menghasilkan rekomendasi teknis terkait standardisasi bahan baku, pencatatan produksi, sanitasi, pengendalian mutu, dan kesiapan pemenuhan standar produksi pangan olahan. Hasil kegiatan menunjukkan bahwa kolaborasi akademik–industri dapat menjadi model hilirisasi inovasi berbasis rumput laut yang mendukung peningkatan kapasitas mitra, mutu produk, dan keamanan pangan.
Co-Authors - Afrizal ANDRIANI ANDRIANI Antony Lay Aulanni ‘am Aulanni ‘am Bambang Sugiharto BAYU ARYA BAGASKARA Ciawi - DARWIN SILALAHI DEWA AYU RATIH Dewi, Ni Kadek Emi Sintha Dharmawibawa I.D. DINA RAHMAWATI Evy Prima FADEL ALKAHFI Gayatri, Anak Agung Sagung Intan Gresia Eldina Haryadi, Celvin Hengky Surya Bhuana I Gede Ketut Susrama I Gusti Ayu Diah Yuniti I Ketut Siadi I Ketut Suada I Ketut Suada I M. Sudarma I Made Parama Nanda Mahatya I N. WIJAYA I NYOMAN MANTIK ASTAWA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I NYOMAN WIJAYA I Putu Agus Hendra Wibawa I Putu Anom I Putu Sudiarta I Putu Wahyu Sanjaya I WAYAN PRASTITA DIASTAMA I WAYAN SUAMBA I.N. Wijaya Ida Ayu Putri Darmawati IDA BAGUS GDE PRANATAYANA K. Sri Marhaeni Julyasih K. Suata Kalimutu, Putu Krisnawan Kayo Gouya Ketut Sri Marhaeni Julyasih Ketut Srie Marhaeni Julyasih KHAMDAN KHALIMI Liliek Sulistyowati Linawati Linawati Luh Putu Wrasiati M. Puspawati Made Adnyana Made Heny Urmila Dewi MADE SRITAMIN Merlyna Rosalia Motoyuki Suzuki Munthe, Lambok Jenny Sagita MUSLIM MUSLIM NADIAH SILITONGA Nevy Widya Pangestika Ni Gusti Ayu Dewi Paramita Arisandi NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI MADE ADHYA NIDIDHYA SANI Ni Made Ayuratih Utami Ni Made Suaniti NI NENGAH DARMIATI NI WAYAN ARMINI Ningsih, Gusti Ketut Asti Dea Nobuyuki Kohno Novayolla, Alsha Nurftatmelia Nyoman Agus Bagiada Parwata, I Made Oka Pranata, Kadek Dwipayana Pranatayana, Ida Bagus Gede PUTU BAMBANG IKA WINJALISTA PUTU SENA WIDIATMA Riezchy Riando Mamanua Rindang Dwiyani Rizki, Muhamad Farrel ROMAULI THERESIA NAINGGOLAN Romulia Trielva Apriliani Rusmita, Gst Ayu Putu Ika Yulia Safitri R. Sahanggamu Y. P. Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Setianingsih, Ni Luh Putu Putri Shara Yulita Harianja Shchegolkova, Nataliya Shin-ichi Sekiyama Simarmata, Jonatan Alexius Suniti N.W. Tampubolon, Immanuel Anthonio Caesebo TRISNA AGUNG PHABIOLA VANI SILVANA VEDERIS LEUNARDUS VICKY TANDYA WAYAN ADIARTAYASA WHISNU IDA BAGUS YOLANDA HASSIAN MANALU YUDHA, I KADEK WISMA Yundari, Yundari