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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Journal of Tourism INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Master Pariwisata (JUMPA) Jurnal Biologi Tropis International Journal of Social Service and Research Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Nandur ARIMA : Jurnal Sosial Dan Humaniora Biosaintifika: Journal of Biology & Biology Education
I GEDE PUTU WIRAWAN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Keberadaan Mikroorganisme Tanah pada Areal Rehabilitasi Takino Soil Protection Sheet dan Kemampuan Menahan Erosi Permukaan di Kaldera Gunung Batur I NYOMAN TRYADI CAHYA NUGRAHA; MADE SRITAMIN; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Existence of Microorganism On Rehabilitation Area by Takino Soil Protection Sheet and Capability to Resist Soil Erosion in the Vulcanic Devastated Site in Mt. Batur The goal of this research was to know the existence of microorganism on rehabilitation area of Takino Soil Protection Sheet (TSPS) and capability to resist soil erosion in the vulcanic devastated site of Mt. Batur. This research used total plate count method to obtain mikroorganism colony, soil separating method to obtain mycorrhizal fungi spores, staining root method to find out percentage of mycorrhizal fungi spores on the roots and capability to resist erosion by measuring the soil trapped on the TSPS. The isolation results showed the number of bacteria colonies has been increase as much as 123%, while the number of fungi spores also increase as much as 100% in the area covered by TSPS. The results of trapping mycorrhizal fungi spores on the area covered by TSPS is 63/100g soil, in the area non covered by TSPS is 21/100g soil. The percentage of mychorrizal fungi infection in the plant roots on the area covered by TSPS is 27,5%. The result showed the average capability of TSPS to resist erosion is 5,26 cm over 3 years after implementation of TSPS.
Identifikasi Mikoriza Vesikular Arbuskular (MVA) pada Rhizosfer Tanaman Ubi Jalar (Ipomoea batatas L.) dan Ubi Kayu (Manihot esculenta Crantz) serta Perbanyakannya dengan Media Zeolit PUTU SENA WIDIATMA; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Vesicular Arbucular Mycorrhizae (VAM) Identification of Sweet Potato (Ipomoea batatas L.) and Cassava (Manihot esculenta Crantz) Rhizosphere and Its Spore Multiplication in Zeolite Media Vesicular arbuscular mycorrhizae is an obligate symbiont that live in a symbiotic mutualism with plant roots and grow inside root cortical cells helping absorption of nutrients which necessary for plant growth. This research aimed to determine VAM species in sweet potato and cassava rhizosphere and also to determine effectiveness of zeolite media as a multiplication medium. The research has been conducted from October 2014 through December 2014. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated with gridline section method and spores multiplication through trapping culture method. Results showed that there were four VAM genera consist of 16 species from rhizosphere of both sample plants. Those four genera identified as Acaulospora (2 species), Gigaspora (1 species), Glomus (1 species), and Scutellospora (1 species) from rhizosphere of sweet potato and 3 genera identified as Acaulospora (3 species), Gigaspora (3 species), and Glomus (5 species) from rhizosphere of cassava. Colonization is characterized by special structure of VAM such as arbucule and vesicule in plant roots tissue. Inner spores of VAM were discovered in all three kind of plants used in this research. Spore multiplication with trapping culture method using zeolite media and corn as a symbionts can be considered as an effective method for VAM spore multiplication indicated by density increase of VAM spores. Keywords : Colonization, Cortical Cell, Inner Spore, Symbiont, Trapping Culture
Pengaruh Variasi Waktu Inkubasi dengan Penambahan Paya Meat Tenderizer pada Proses Dekafeinasi Kopi Arabika (Coffea arabica L.) DEWA AYU RATIH; MADE SRITAMIN; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.1, Januari 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effect of Incubation Time Variation with The Addition of Paya Meat Tenderizer in the Arabica Coffee Decaffeination Process (Coffea arabica L.) High levels of caffeine lead to insomnia and increased blood pressure. Decaffeination is a solution to reduce the caffeine with enzymatic method with papain coarse extract PAYA Meat Tenderizer contains proteolytic activity of 1,0593 units/g. The study aims to find out the effect of arabica coffee incubation time with the addition of the enzyme papain PAYA Meat Tenderizer. The study looked at protein levels (Kjeldahl), caffeine levels (UV-Vis spectrophotometry) and organoleptic tests. The design used is Complete Random Design (RAL) 4 incubation time treatment i.e. B0 (0 hours); B1 (12 hours); B2 (24 hours) and B3 (36 hours). The results showed a very real influential treatment of all observed variables. B2 (24-hour incubation) lowers caffeine levels by 0,006%. A 0,12% decrease in protein levels occurred in 36-hour incubation. The highest results on organoleptic tests of aroma, viscosity and bitter taste parameters were obtained control samples (B0) and 36-hour (B3).
Identifikasi Fungi Mikoriza Arbuskular Secara Mikroskopis pada Rhizosfer Tanaman Alang-Alang (Imperata Cylindrica L.) di Desa Sanur Kaja ROMAULI THERESIA NAINGGOLAN; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Identification of Mikoriza Arbuskular Fungi in Microscopic the Rhizosphere of Reed (Imperatacylindric L.) in Sanur Kaja Village.   Mycorrhiza is a form of symbiosis between the fungus with a high level of plant (vascular plants, Tracheophyta), especially on the rooting. There is also a fungus with the other symbiotes, but the term mycorrhiza are typically for symbiotic plant roots which infect by fungus. This research aims to know the diversity of the genus or species of  mycorrhiza fungi arbuskular on the rhizosfer plant reeds in the village of Sanur Kaja, and to know whether or not there was colonization of mycorrhiza fungi arbuskular on the net root crops the reeds in the village of Sanur Kaja. The results of this research show that the symbiotes spores in the rhizosfer plant reeds is the genus Glomus multicaule spores, Glomus ambisporum, Acaulospora foveata, Gigaspora gigantae. Analysis on the plant roots mycorrhiza colonization reed showed a spherical structure called vesikular, while arbuskular is a structure on the hypha branches that resemble haustorium. (forming the pattern dikotom).   Key words: Acaulospora sp, Gigaspora sp, Glomus sp.
Pengamatan Gejala Penyakit CVPD pada 12 Spesies Tanaman Jeruk VEDERIS LEUNARDUS; I GEDE PUTU WIRAWAN; I KETUT SUADA; I GUSTI AYU DIAH YUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Diseases Symptoms Observation at 12 Species of Citrus Plants This study was conducted to observe symptoms that showed by citrus plantswhich already tested by PCR carry CVPDr DNA Fragment. CVPDr is resistance geneto prevent plant from CVPD diseases. Based on observations in the field showed thatfive from twelve species showed the symptoms even already carry the gene. Five ofthem are Citrus reticulata Selayer Buleleng, Citrus nobilis Buleleng, Citrus nobilisPecatu, Citrus nobilis Tabanan, dan Citrus reticulata Keprok Gianyar. Mutationanalysis and homology analysis should be done after this observation to know whythis gene is not functional to prevent CVPD diseases.
Analisis Fitokimia Ekstrak Bulung Boni (Caulerpa spp.) dan Uji Daya Hambatnya terhadap Fungi Fusarium moniliforme (Sacc.) Nirenberg VICKY TANDYA; I GEDE PUTU WIRAWAN; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Phytochemical Analysis Of Bulung Boni Extract (Caulerpa spp.) and Its Inhibiting Ability Against Fusarium moniliforme (Sacc.) Nirenberg Seaweed is a marine biota that produces bioactive compounds which are useful for the development of the pharmaceutical industry. Caulerpa spp. is a green algae that is commonly consumed as vegetables or fresh vegetables by tropical communities, but the use of Caulerpa spp. in other fields is not optimal. The purpose of this study was to determine the content of antifungal compounds in the extract of Caulerpa spp. and antifungal activity against F. moniliforme. The research method used was Gas Chromatography and the diffusion well method with 14 treatments and 3 replications. The design used was completely randomized with ANOVA analysis followed by Duncan's test with a confidence level of 5%. The results showed that the ethanol is extract of Caulerpa spp. has antifungal compounds, namely alkaloids, saponins, and organic acids which indicated fungistatic effect. Ethanol extract of Caulerpa spp. had minimal antifungal activity (MIC) against F. moniliforme at a concentration of 0.6% with an inhibition zone diameter of 5 mm and was categorized as weak. The best extract concentration in inhibiting F. moniliforme in the treattested was a concentration of 5% with an inhibition zone diameter of 13.67 mm and a percentage was 54.54% colony inhibition.
Isolasi dan Identifikasi Fungi Mikoriza Arbuskular (Fma) secara Mikroskopis pada Rhizosfer Tanaman Jeruk (Citrus sp.) di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar I WAYAN SUAMBA; I GEDE PUTU WIRAWAN; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Arbuscular Mycorrhizal Fungi (AMF) by Microscopic (AMF) in Rhizosphere of Citrus Plant (Citrus sp.) in Kerta Village, Payangan District, Gianyar Regency Arbuscular Mycorrhizal Fungi (AMF) is a group of organisms from fungi that describes a form of mutualism between symbiotic fungi and plant roots. AMF has great potential as a biological fertilizer because the microorganism which has a very important role in facilitating the absorption of nutrients in the soil to increase plant growth, in addition AMF also function as a biological barrier against pathogen that infect the roots, increasing the availability of water for plants to grow and improve hormone boosters. The purpose of the study was to determine the types of AMF that exist in the rhizosphere of citrus plant and determine the presence of infection by the AMF in the citrus plant roots in the  village of Kerta. The results of the isolation and identification of AMF spores in the rhizosphere of citrus plants in the village of Kerta found 14 different types of AMF spores. This study found 7 type of Glomus, 5 types of Gigaspora and 2 types of Acaulospora. Those types were determined by using the characteristic of their spores. Observations on root finding that the AMF  infection in the form vesicles and arbuscular structures. Keywords : Arbuscular Mycorrhizal Fungi, Glomus, Gigaspora, Acaulospora, Vesicles and Arbuscular.
Isolasi dan Identifikasi Agrobacterium tumefaciens dari Tanaman Wortel (Daucus carota L.) YOLANDA HASSIAN MANALU; I GEDE PUTU WIRAWAN; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens from  Carrots (Daucus carota L.) Agrobacterium tumefaciens is a plant pathogenic bacterium that is widely used as a vector for inserting foreign genes into a plant chromosome to produce a transgenic plant. This bacterium cause a disease namely crown gall in dicotyledonous plants. This study is aim to isolate A. tumefaciens from local carrot (Daucus carota L.) by using some method such as selection media, characterization of the bacteria, and confirm by Koch’s postulates. The result of this study determined that carrot’s root taken from Bedugul area which showed crown gall symptom, an A. tumefaciens due to its characteristics e.g. shape of colonies, color, and growth of the bacteria in AB minimal medium that was a specific for A. tumefaciens.  By using the Koch’s postulates test showed that the isolated caused convex gall on the surface of carrot slice. DNA of isolated bacterium successfully electroforeted through agarose gel electrophoresis.  These results showed that the bacteria associated with carrot was A. tumefaciens. Keywords : A. tumefaciens, AB medium, Carrot, LB medium, Crown gall
Deteksi Keberadaan Liberobacter asiaticum Pada Tanaman Jeruk Yang Terserang Citrus Vein Phloem Degeneration (CVPD) Dengan Gejala Parsial VANI SILVANA; I NYOMAN WIJAYA; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Detection Of The Existence of Liberobacter Asiaticum in Citrus Stricken with Sympthoms of Partial CVPD Citrus Vein Phloem Degeneration (CVPD) is a major disease of citrus caused by Gram-negative bacteria Liberobacter asiaticum. The symptoms of the disease were varied due to many factors such as type of pathogenic races of the pathogen, types of citrus trees, and the presence or spread of plant pathogens in the plant. This study aims to detect the existence or distribution of pathogens of CVPD in citrus plant with partial symptoms. Samples were taken from the bone leaves, bark twigs, bark of branches and bark of stem whether symptomatic or non symptomatic. Sampling was carried out in Plaga village, Badung Regency, Bali and analysis of polymerase chain reaction (PCR) was conducted in UPT Lab Genetics Resources and Molecular Biology, Udayana University. The results of this study indicate that the amplified DNA of 1160 bp was found in the first plant that was  symptomatic leaf, twig bark of leaf symptomatic and non symptomatic leaves. While on the second sample of citrus plants, DNA fragments were found on the leaves and symptomatic leaf and non symptomatic, and they were not found in other parts of plant, namely twig bark, branch bark, bark of stem. These results indicate that in citrus with partial symptoms the distribution of pathogens L. asiaticumwere only partial in plant.
Analisis Homologi Fragmen DNA CVPDr dari Jeruk Kinkit Trophasia trifolia Menggunakan BLAST Protein Dan BLAST Nukleotida WHISNU IDA BAGUS; I GEDE PUTU WIRAWAN; I WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Homology Analysis Of CVPDr DNA Fragment from 13 Citrus Variety in Bali Using Nucleotide Blast And Protein Blast CVPDr from Trhiphasia trifolia is predicted to have to increase resistance fromCVPD, but the mechanism of this DNA is still largely unknown. The first step of DNAanalyzing is by using homology with Nucleotide and Protein BLAST. BLAST canidentify, similarity search, and predict the function of DNA. The goal of this researchis to compare CVPDr DNA fragment from T. Trifolia with sequences from NCBIGenbank and finding it’s significant homology using nucleotide BLAST and proteinBLAST. Nucleotide BLAST result shows that “DNA Citrus nobilis cultivar Tabanancitrus vein phloem degeneration r protein (CVPDr) gene, partial cds” have the highesthomologs, the other sequences that’s shown by BLAST does not have high enoughsimilarity to be homologus. Protein BLAST show “citrus vein phloem degeneration rprotein [Citrus nobilis]” have the highest homologs, but even if the other BLAST resulthave percentage of similarity lower than 55% there are still possibility of structuralhomolog because the E value is lower than 0.01.
Co-Authors - Afrizal ANDRIANI ANDRIANI Antony Lay Aulanni ‘am Aulanni ‘am Bambang Sugiharto BAYU ARYA BAGASKARA Ciawi - DARWIN SILALAHI DEWA AYU RATIH Dewi, Ni Kadek Emi Sintha Dharmawibawa I.D. DINA RAHMAWATI Evy Prima FADEL ALKAHFI Gayatri, Anak Agung Sagung Intan Gresia Eldina Haryadi, Celvin Hengky Surya Bhuana I Gede Ketut Susrama I Gusti Ayu Diah Yuniti I Ketut Siadi I Ketut Suada I Ketut Suada I M. Sudarma I Made Parama Nanda Mahatya I N. WIJAYA I NYOMAN MANTIK ASTAWA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I NYOMAN WIJAYA I Putu Agus Hendra Wibawa I Putu Anom I Putu Sudiarta I Putu Wahyu Sanjaya I WAYAN PRASTITA DIASTAMA I WAYAN SUAMBA I.N. Wijaya Ida Ayu Putri Darmawati K. Sri Marhaeni Julyasih K. Suata Kalimutu, Putu Krisnawan Kayo Gouya Ketut Sri Marhaeni Julyasih Ketut Srie Marhaeni Julyasih Liliek Sulistyowati Linawati Linawati Luh Putu Wrasiati M. Puspawati Made Adnyana Made Heny Urmila Dewi MADE SRITAMIN Merlyna Rosalia Motoyuki Suzuki Munthe, Lambok Jenny Sagita MUSLIM MUSLIM NADIAH SILITONGA Nevy Widya Pangestika Ni Gusti Ayu Dewi Paramita Arisandi NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI MADE ADHYA NIDIDHYA SANI Ni Made Ayuratih Utami Ni Made Regina Prasetya Putri Ni Made Suaniti NI NENGAH DARMIATI NI WAYAN ARMINI Ningsih, Gusti Ketut Asti Dea Nobuyuki Kohno Novayolla, Alsha Nurftatmelia Nyoman Agus Bagiada Parwata, I Made Oka Pranata, Kadek Dwipayana Pranatayana, Ida Bagus Gede PUTU BAMBANG IKA WINJALISTA PUTU SENA WIDIATMA Riezchy Riando Mamanua Rindang Dwiyani Rizki, Muhamad Farrel ROMAULI THERESIA NAINGGOLAN Romulia Trielva Apriliani Rusmita, Gst Ayu Putu Ika Yulia Safitri R. Sahanggamu Y. P. Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Setianingsih, Ni Luh Putu Putri Shara Yulita Harianja Shchegolkova, Nataliya Shin-ichi Sekiyama Simarmata, Jonatan Alexius Suniti N.W. TRISNA AGUNG PHABIOLA VANI SILVANA VEDERIS LEUNARDUS VICKY TANDYA WAYAN ADIARTAYASA WHISNU IDA BAGUS YOLANDA HASSIAN MANALU Yundari, Yundari