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All Journal PHARMACON Jurnal Ilmu Kefarmasian Indonesia The Studies of Social Sciences Jurnal Kesehatan Tambusai Jurnal Ilmiah Sains Grimsa Journal of Science Engineering and Technology Jurnal Lentera Farma

Paulina yamlean

Program Studi Farmasi, Universitas Sam Ratulangi

Author-ID : 421568

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN SABUN WAJAH CAIR EKSTRAK ETANOL BUAH PARE (Momordica Charantia L.) TERHADAP BAKTERI Staphylococcus Epidermidis Marhaba, Fera Anelia; Yamlean, Paulina V.Y.; Mansauda, Karlah L.R.
PHARMACON Vol 10, No 3 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.35609

ABSTRACTBitter melon (Momordica Charantia L.) contains flavonoid compounds, alkaloids, and saponins which have antibacterial properties. The perpose of this study was to formulate the ethanolic extract of bitter melon into liquid facial soap preparations and to test the antibacterial effectiveness of liquid facial soap preparations against bacteria Staphylococcus epidermidis. This study have done by using laboratory experimental methods. Antibacterial effectiveness of bitter melon extract liquid facial soap was tested using the well diffusion method. Based on the results of the study, it showed that physical evaluations such as organoleptic tests produced (Odor: typical apple scent, color: green, texture: liquid), pH test was 5.5, foam height measurement was 60-62 mm, specific gravity test was 1.03 -1,05 g/ml , free alkali level test was 0.05%-0.08% , water content test was 32-38%. The liquid facial soap obtained from the ethanol extract of bitter melon fruit can inhibitbacteria Staphylococcus epidermidis with strong category in concentration of 2.5% (10.16 mm); 5% (11.58 mm); 7.5% (12.5 mm); 10% (13 mm) and 12.5% (14 mm). It can be concluded that the ethanol extract bitter melon can be formulated into a liquid facial soap that has good physical quality and has the greatest inhibitory power in concentration 12.5%.Â Keywords: Bitter melon, Liquid Facial Soap, Evaluation of Physical Properties, Antibacterial.Â Â ABSTRAKÂ Buah Pare (Momordica Charantia L.) memiliki kandungan senyawa flavonoid, alkaloid, dan saponin yang berkasiat sebagai antibakteri. Penelitian yang dilakukan bertujuan untuk memformulasikan ekstrak etanol buah Pare menjadi sediaan sabun wajah cair dan menguji efektivitas antibakteri sediaan sabun wajah cair terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode eksperimental laboratorium. Pengujian efektivitas antibakteri sabun wajah cair ekstrak buah pare menggunakan metode difusi sumuran. Berdasarkan hasil penelitian, menunjukkan evaluasi fisik seperti uji organoleptik menghasilkan (Bau: khas pengaroma apel, warna: hijau, tekstur: Cair), Uji pH yaitu 5,5, Pengukuran tinggi busa yaitu 60-62 mm, uji bobot jenis yaitu 1,03-1,05 g/ml , uji kadar alkali bebas yaitu: 0,05%-0,08%, uji kadar air yaitu 32-38%. Sabun wajah cair ekstrak etanol buah Pare yang diperoleh dapat menghambat bakteri Staphylococcus epidermidis dengan kategori kuat pada konsentrasi 2,5% (10,16 mm); 5% (11,58 mm); 7,5% (12.5 mm); 10% (13 mm) dan 12,5% (14 mm). Dapat disimpulkan bahwa ekstrak etanol buah Pare dapat diformulasikan menjadi sediaan sabun wajah cair yang memiliki mutu fisik yang baik dan memliki daya hambat yang paling besar pada konsentrasi 12,5%.Â Kata kunci: Buah Pare, Sabun Wajah Cair, Evaluasi Sifat Fisik, Antibakteri.

FORMULASI SEDIAAN SABUN MANDI PADAT EKSTRAK ETANOL DAUN GEDI (Abelmoschus manihot L.) TERHADAP BAKTERI Staphylococcus epidermidis Mopangga, Elisticia; Yamlean, Paulina V.Y.; Abdullah, Surya Sumantri
PHARMACON Vol 10, No 3 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.35605

ABSTRACTGedi leaves (Abelmoschus manihot L.) contain metabolite compounds such as flavonoid, saponins, tannins and terpenoids for antibacterial. This study aims to formulate a solid bath soap with concentrations of 2%, 2,5% and 3% of Gedi leaves ethanol extract and to test the antibacterial activity of solid bath soap against Staphylococcus epidermidis bacteria. Experimental methods were performed for this research. For the formulation of this solid soap several experiments were conducted organoleptic test, pH, homogeneity, moisture content, free alkaline, and high foam. The effectiveness of antibacteria to inhibit Staphylococcus epidermidis growth was tested by wells method. The results showed that the ethanol extract of Gedi leaves (Abelmoschus manihot L.) can be formulated into solid bath soap that meets the requirements according to the standards set to SNI (Indonesian National Standard) with the results of physical tests of all formulas in solid or hard form and scent of rose, the distribution of colors is evenly distributed, has a pH of 8,32-9,09, a moisture content of 8,65%-8,84%, foam height from 3,7-3,8 cm, and free alkaline are in a safe condition against the skin. The results of the activity of antibacteria solid bath soap extract of the Gedi leaves were found to inhibit Staphylococcus epidermidis bacteria which has the strongest inhibition power at a concentration of 3% with an inhibition zone diameter of 14,8 mm.Â Keywords: Gedi Leaves (Abelmoschus manihot L.), Solid Soap, Antibacterial, Staphylococcus epidermidis. Â ABSTRAKDaun Gedi (Abelmoschus manihot L.) memiliki kandungan senyawa flavonoid, saponin, tanin dan terpenoid yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sediaan sabun mandi padat ekstrak etanol daun Gedi dengan konsentrasi 2%, 2,5% dan 3% serta menguji aktivitas antibakteri sediaan sabun mandi padat terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Uji mutu sediaan sabun mandi padat ekstrak etanol daun Gedi dilakukan uji organoleptik, pH, homogenitas, kadar air, alkali bebas, dan tinggi busa. Pengujian aktivitas antibakteri terhadap pertumbuhan Staphylococcus epidermidis dilakukan dengan metode sumuran. Hasil penelitian menunjukkan ekstrak etanol daun Gedi (Abelmoschus manihot L.) dapat diformulasikan menjadi sediaan sabun mandi padat yang memenuhi persyaratan sesuai dengan standar yang ditetapkan SNI (Standar Nasional Indonesia) dengan hasil semua formula berbentuk padat atau keras dan beraroma rose, penyebaran warna merata, memiliki pH 8,32-9,09, kadar air 8,65%-8,84%, tinggi busa 3,7â€“3,8 cm, serta alkali bebas dalam kondisi aman terhadap kulit. Hasil uji aktivitas antibakteri sediaan sabun mandi padat ekstrak etanol daun Gedi yang diperoleh dapat menghambat bakteri Staphylococcus epidermidis yang memiliki daya hambat terkuat pada konsentrasi 3% dengan diameter zona hambat 14,8 mm.Â Kata kunci: Daun Gedi (Abelmoschus manihot L.), Sabun Padat, Antibakteri, Staphylococcus epidermidis

FORMULASI SEDIAAN GRANUL EFFERVESCENT SARI BUAH SIRSAK (Annona muricata L) Burhan, Lisma; Yamlean, Paulina V. Y.; Supriati, Hamidah Sri
PHARMACON Vol 1, No 2 (2012)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.1.2012.489

Soursoup fruit nutrient includes vitamins C and B1. Vitamin which is the most dominant insoursop fruit is vitaman C, where the compound is in the form of a natural antioxidant in thebody. Antioxidants help the body to fight against various free radicals that enter the body and isexcellent for improving the immune system and slow the aging process. This study aimed toinvestigate the effect of different formulations of effervescent granules made from soursoup fruitjuice against physical properties of granules prepared by wet granulation method. Testpreparation includes organoleptic quality, moisture content, flow properties, and solubility time.Of the three formulations, variations in the quantity of citric acid, tartaric acid and sodiumbicarbonate gave effect on water levels, silence angle, and time of depression of granule made ofsoursoup fruit juice. In formulation 1, the water content was 0.19%, a flow rate of 8.2 seconds,the silence angle of 33.02 degrees, and dispersion time was 2 minutes 13 seconds.In the secondformulation, water content was 0,21%, a flow rate of 8.1 seconds, the silence angle of 31.52degrees, and dispersion time at 3 minutes 3 seconds. In the third formulation, water content was0,28%, a flow rate of 9,7 seconds, the silence angle of 31.56 degrees, and dispersion time at 4minutes 5 seconds.Key words: soursoup, effervescent granule, wet granule, natrium bicarbonate

UJI EFEK ANTIINFLAMASI EKSTRAK ETANOL DAGING BUAH LABU KUNING (Cucurbita moschata D.) TERHADAP EDEMA PADA TELAPAK KAKI TIKUS PUTIH JANTAN GALUR WISTAR (Rattus novergicus) Senewe, Merry; Yamlean, Paulina V.Y; Wiyono, Weny
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.1016

ABSTRACT Inflamation is a protective response of body from injury of cell.Edema is an indication of inflammation.Pumpkin pericarp contains of flavonoid which has potency to decrease edema.The aims of this research were to study antiinflamation effect of ethanolic extract of pumpkin pericarp (Cucurbita moschata D.) with concentration 5, 10 and 20%on edema of white rat legs strain wistar.The subjects in this research were 15 white male rat strain wistar which divided into 5 groups, namely negative control (CMC),Â positive control (Piroksikam) and extract group with concentration 5, 10 and 20%.Induction of 5% of chicken albumin on rats left leg lead to edema formation, followed with induction of CMC, Piroksikam solution and extractper oral. Measurement of edema volume was done using plestimometer, and observation of edema were made every 6 hours. The result shows that there were significant administration effects of pumpkin pericarp extract with concentration 5 and 10%. As a conclusion, ethanol extract of pumpkin pericarp exhibit potency to decrease edema.

Variasi Basis Salep Minyak Kemiri (Aleurites moluccana) dan Uji Daya Penyembuhannya Pada Luka Kelinci Paulina Veronika Yamlean; Edwin De Queljoe; Widdhi Bodhi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Candlenut plant in Indonesia is still limited to traditional uses such as cooking spices and traditional medicines. Only a few research has been done on Candlenut oil. The purpose of this study is to make ointment base variations toward the quality of Candlenutoil ointment with 12.5% concentration andits healing potential teston open wounds in rabbits infected with the Staphylococcus aureus bacteria. This research method is a laboratory experiment using 3 variations of base ointment namely hydrocarbon base, absorption base and water soluble base with Candlenut oil test solution of 12.5%. The results showed the quality test of the ointment with three variations of ointment base fulfilled the requirements of organoleptic test, homogeneity test, and pH test, but Candlenutoil ointment of 12.5% with hydrocarbon base did not meet the scattering power requirement. The results of the ANOVA test showed no significant difference between the base variations of the ointment and the size of wound healing. This shows that the three variations of the 12.5% Candlenut oil ointment base can be used in healing open wounds on rabbits (Oryctolagus cuniculus) infected with the Staphylococcus aureus bacteria.

IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B PADA JAJANAN KUE BERWARNA MERAH MUDA YANG BEREDAR DI KOTA MANADO Paulina V.Y Yamlean
JURNAL ILMIAH SAINS Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B PADA JAJANAN KUE BERWARNA MERAH MUDA YANG BEREDAR DI KOTA MANADO Paulina V. Y. Yamlean1) 1)Program Studi Farmasi FMIPA Universitas Sam Ratulangi Manado, 95115; Email:olan_0506@yahoo.co.id ABSTRAK Rhodamin B adalah pewarna terlarang yang sering ditemukan pada makanan, terutama makanan jajanan. Rhodamin B, yaitu zat pewarna berupa serbuk kristal berwarna hijau atau ungu kemerahan, tidak berbau, serta mudah larut dalam larutan warna merah terang berfluoresan sebagai bahan pewarna tekstil atau pakaian. Jenis jajanan yang banyak dijumpai dan dicampuri dengan Rhodamin B, antara lain bubur delima, cendol, kolang-kaling, cincau dan kue-kue lainnya. Setelah dicampuri bahan ini makanan tersebut menjadi berwarna merah muda terang. Hasil penelitian yang diperoleh membuktikan bahwa sampel-sampel kue berwarna merah muda yang beredar di kota Manado ada yang positif menggunakan Rhodamin B. Kata kunci: kue berwarna merah muda, rhodamin B Â IDENTIFICATION AND DETERMINATION LEVEL OF RHODAMIN B ON STREET FOOD PINC CAKE THAT CIRCULATION TO MANADO CITY ABSTRACT Rhodamin B is the illegal dyes that is often found in food, especially street foods. Rhodamin B, which is a dye form crystalline powder colored green or reddish purple, odorless, easily soluble inÂ bright red fluoroscence solution as dye of textiles or apparel. Types of street food that are often found and mixed with Rhodamin B, amon pomegranate porridge, cendol, fro, grass jelly and other pastries. After mix with Rhodamon B, all that food becomes light pink. The results of research has obtained prove that the samples pink cake that circulated in Manado citythere are positive use of Rhodamin B. Keywords: pink cake, rhodamin B

Analisis Fitokimia dan Uji Toksisitas Daun Leleng Merah (Graptophyllum pictum (L.) Griffith) dengan Metode Brine Shrimp Lethality Test Defrikson Ba'u; Dewa G Katja; Vanda S Kamu; Paulina V.Y Yamlean; Max R.J Runtuwene
JURNAL ILMIAH SAINS Volume 20 Nomor 2, Oktober 2020
Publisher : Sam Ratulangi University

Penelitian ini bertujuan untuk menentukan total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC) dan nilai toksisitas dari daun leleng merah. Daun leleng merah diekstraksi dengan metode maserasi dan dipartisi menggunakan pelarut n-heksana, etil asetat, dan air. Penentuan nilai TPC menggunakan metode Folin-Ciocalteu. Penentuan nilai TFC menggunakan metode AlCl3. Penentuan nilai CTC menggunakan metode vanillin-HCl. Penentuan nilai toksisitas menggunakan metode BSLT. Nilai TPC yang diperoleh pada fraksi n-heksana (FH), fraksi air (FA), dan fraksi etil asetat (FEA) daun Leleng Merah berturut-turut, adalah: 20,48; 31,7; dan 37,57 (mg/g). Nilai TFC diperoleh pada FH, FA, dan FEA berturut-turut, adalah: 0,64; 0,99; dan 1,27 (mg/g). Nilai CTC diperoleh pada FH, FA, dan FEA berturut-turut, adalah: 0,56; 2,17; dan 6,52 (mg/g). Nilai toksisitas diperoleh pada FEA, FA, FH berturut-turut, adalah: 21,93; 100,7; dan 117,22 (mg/L). FEA memiliki nilai toksisitas dan kandungan fitokimia tertinggi. Daun leleng merah bersifat toksik.Kata Kunci : Brine shrimp lethality test, leleng merah, fitokimia,Â Phytochemical Analysis and Toxicity Test of Leleng Merah Leaves (Graptophyllum pictum (L.) Griffith) Using the Brine Shrimp Lethality TestÂ ABSTRACTThis study aims to determine the total phenolic content (TPC), flavonoids (TFC), condensed tannins (CTC) and toxicity value of Leleng Merah leaves. Leleng merah leaves were extracted by maceration method and partitioned using n-hexane, ethyl acetate, and water solvent. TPC values were determined using the Folin-Ciocalteu method. TFC values were determined using the AlCl3 method. CTC values were determined using the vanillin-HCl method. Toxicity values were determined using the BSLT method. TPC values obtained in the n-hexane (FH), water (FA), and ethyl acetate (FEA) fraction of the Leleng Merah leaves, were: 20.48; 31.7; 37.57 (mg / g) respectively. TFC values obtained in FH, FA, and FEA, were : 0.64; 0.99; 1.27 (mg / g) respectively. CTC values obtained at FH, FA, FEA, were: 0.56; 2.17;Â 6.52 (mg/g) respectively. Toxicity values obtained at FEA, FA, FH, were: 21.93; 100.7; 117.22 (mg/L) respectively. FEA has the highest toxicity and phytochemical content. Leleng Merah Leaf is toxic.Keywords : Brine Shrimp Lethality Test, leleng merah, phytochemistry

FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF COCOA BEAN EXTRACT TOOTHPASTE (Theobroma Cacao L.) AGAINST STREPTOCOCCUS MUTANS BACTERIA Apriliani Margaret Marista Lourina Rarung; Paulina Yamlean; Karlah Mansauda
PHARMACON Vol. 11 No. 4 (2022): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cocoa beans (Theobroma cacao L.) are seeds of the cocoa tree which have a active components, is polyphenols which have the potential as antibacterial. This study aimed to formulate the toothpaste of Cocoa bean ethanol extract with concentrations of 5%, 7.5%, 10%, 12.5%, 15% and tested for physical evaluation of the preparation and to obtain the inhibitory power against Streptococcus mutans bacteria. In this research, the cocoa beans was extracted used the maceration method with 96% ethanol solvent and tested for antibacterial used the well method. The results showed an organoleptic physical evaluation requirements, homogeneous homogeneity test, pH test 7.4-7.7, spreadability test 30-33 mm, and adhesion test 2.25-3.23 seconds. Toothpaste has antibacterial activity with an average diameter of F1 (8.16 mm), F2 (8.5 mm), F3 (9.5 mm), F4 (10.41 mm) are in the medium category and F5 (11.58 mm) is in the strong category. Based on the study result can be concluded that all formulations of toothpaste preparations of Cocoa bean ethanol extract filled the antibacterial activity requirements and physical test requirements and had the strongest antibacterial activity at a concentration of 15% at 11.58 mm.

Formulation and Antibacterial Activity Test of Peel-Off Gel Mask Ethyl Acetate Fraction of Cocoa Pods (Theobroma cacao L.) against Staphylococcus aureus as Anti-Acne Immanuela Irene Mandias; Paulina Yamlean; Surya Sumantri Abdullah
PHARMACON Vol. 11 No. 4 (2022): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cocoa pods (Theobroma cacao L.) is a part of the Cocoa plant which contains active compounds such as flavonoids, alkaloids, saponins and tannins that have the potential as antibacterial. This study aimed to formulate a peel-off gel mask preparation of the ethyl acetate fraction which is physically good and to test the inhibitory power of the peel-off gel mask with concentrations of 2, 4, 8, 10, and 12% against Staphylococcus aureus. This research used laboratory experimental method. The results showed that the peel-off gel mask filled the requirements for physical evaluation, that is organoleptic, homogeneity, pH, spreadability, adhesion and drying time. The antibacterial activity test using the well diffusion method showed that the average diameter for F1 to F5 respectively was 7,5; 8,58; 12; 12,75; and 14 mm. Based on the result of the study it can be included that the overall formulation of the peel-off gel mask filled the requirements of the parameters of the physical properties of the preparation and provides the greatest antibacterial activity that is formula 5 with a concentration of 12%, which is 14 mm.

PENGARUH GLISERIN TERHADAP STABILITAS FISIK GEL EKSTRAK ETANOL SARI BUAH TOMAT (Solanum lycopersicum L.) Gusti Ayu Wulandari; Paulina Veronika Yolanda Yamlean; Surya Sumantri Abdullah
Jurnal Kesehatan Tambusai Vol. 4 No. 3 (2023): SEPTEMBER 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v4i3.16601

Gliserin pada formula gel berfungsi sebagai humektan, yang mampu mengikat air dan dapat melembabkan kulit, serta berperan dalam menjaga kandungan air dari dalam gel sehingga gel akan lebih stabil. Penelitian ini dilakukan untuk mengetahui bagaimana pengaruh penambahan gliserin (5, 10, 15, 20, 25%) pada sediaan gel ekstrak etanol sari buah tomat terhadap stabilitas fisik meliputi homogenitas, organoleptik, daya sebar, daya lekat dan pH selama masa penyimpanan 21 hari. Metode yang digunakan dalam proses ekstraksi yaitu dengan metode maserasi, kemudian dilanjutkan dengan pembuatan sediaan gel, evaluasi sediaan dan analisa data menggunakan SPSS. Nilai IC50 dari ekstrak sari buah tomat yaitu 16,423 mg/L dan dikategorikan dalam penghambatan terhadap aktivitas radikal bebas yang sangat tinggi. Hasil penelitian pada Formula 1, Formula 2, Formula 3, Formula 4 dan Formula 5 tidak terdapat perbedaan yang signifikan pada nilai daya sebar sebelum dan sesudah penyimpanan dengan nilai sig. > 0,05 pada uji paired T-Test yaitu 0,175; 0,150; 0,266; 0,122 dan 0,130 berturut-turut, serta pada nilai daya lekat sebelum dan sesudah penyimpanan dengan nilai sig. > 0,05 pada uji paired T-Test yaitu 0,059; 0,232; 0,086; 0,062 dan 0,155 berutut-turut. Penambahan gliserin mempengaruhi nilai daya sebar dan daya lekat sebelum dan sesudah penyimpanan pada masing-masing formula, dengan penurunan daya sebar dan daya lekat yang paling sedikit yaitu pada Formula 5 dengan konsentrasi gliserin sebanyak 25% dengan penurunan sebesar 4,66% dan 14,46% berturut-turut.

Co-Authors Aaltje Manampiring Abdullah, Surya S. Abdullah, Surya Sumantri Abubakar, Poetry M.S. Abubakar, Poetry Melinda Adeanne Wullur Adithya Yudistira Amanda Putri Pratikto Anisa Puspa Juwita Antasionasti, Irma Apriliani Margaret Marista Lourina Rarung Ariem, Feiby Ba'u, Defrikson Billy Kepel Christania A. E. Pakasi Clements, Garry Daimunon, Regita Defrikson Ba'u Dewa G Katja Dewa Gede Katja Edi Suryanto Edwin de Queljoe Edwin De Queljoe Ekawati Tallei, Trina Elly Suoth Ering, Mariando N. Fabiola N Palobo Fadillah Djafar Falugah, Fathia Fatimawali . Fona Budiarso Frendsiane Pangalinan Gayatri Citraningtyas Grace Riani Pongsipulung Gratia, Berta Gusti Ayu Wulandari Hamdiyah Hamzah Hamidah Sri Supriati Hosea Jaya Edy Hutauruk, Hamido Immanuela Irene Mandias Inriani MarlinMareyke Rumayar Jainer P. Siampa Jainer Pasca Siampa Jainer Siampa Jayanto, Imam Jeane Mongi Jimmy Posangi Jonly Piere Uneputty Julianri Sari Lebang, Julianri Sari Kalalo, Tekla Karlah L. R. Mansauda Korompis, Freisy C.C Lahagina, Juan Lahagina, Juan C. G. Lakoro, Julia Elsa Lebang, Julianri S. Legoh, Dina Imorina Lempoy, Sela S Liempepas, Angelika Lisma Burhan Lolowang, Sheren N. Lomboan, Evander Roliand Majid, Nurul Majid, Nurul S Manarisip, Thesya Marhaba, Fera Anelia Maureen Regina Satolom Max R. J. Runtuwene Max R.J Runtuwene Max R.J. Runtuwene Merry Senewe Mery A R Sinaga Moilati, Veronica O. Monibala, Tiarma Mopangga, Elisticia Nau'e, Dwi A.K. Nelwan, Sabine A. Niswah Paju Novel Kojong Olivia H Naibaho, Omega Agral Pakasi, Christania A. E. Pakpahan, Kevin Yosua Pangemanan, David Albert Pingkan, Aprilia Pogaga, Eklesia Poluan, Omega A Pratasik, Meyla Pratasik, Meyla C. M. Purwoko, Agus Ratte, Titah Amelia Ravael Kurnia Kolibu Risma Meidy Hardina Sitorus Rompis, Ferrna Rumayar, Ricky C. Rundengan, Gerald Runtuwene, Kristianus Siampa, Jainer Pasca Singal, Ausich Sukandi, Gabrilia Sukandi, Gabrilia P. C. Suru, Eunike Surya S. Abdullah Surya Sumantri Abdullah Surya Sumantri Abdullah Tasya Mangkey Tiarma Tiarma TRINA EKAWATI TALLEI Umboh, Defritsevani Umboh, Defritsevani Y. Vanda S Kamu Vanda S Kamu Veren Naftalia Mamangkey Violeta Gavelentri Melga Tambingon Virginia lasut Walewangko, Marfincy Walewangko, Marfincy S. Weny I Wiyono Weny Wiyono Widdhi Bodhi Widdhi Bodhi Widya Astuty Lolo, Widya Astuty Yos Banne Yuni Arista N Kumesan

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