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All Journal JURNAL KIMIA SAINS DAN APLIKASI Journal of Mathematical and Fundamental Sciences Indonesian Journal of Pharmaceutical Science and Technology Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry JSFK (Jurnal Sains Farmasi & Klinis) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Jurnal Ilmu Kefarmasian Indonesia Berkala Ilmiah Mahasiswa Farmasi Charity : Jurnal Pengabdian Masyarakat Jurnal Farmasi dan Sains Indonesia (JFSI) Medical Sains : Jurnal Ilmiah Kefarmasian Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Eduvest - Journal of Universal Studies Borneo Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Arry Yanuar
Departemen Farmasi Universitas Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

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Journal : Makara Journal of Science

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SKRINING GEN GLUKOSILTRANSFERASE (GTF) DARI BAKTERI ASAM LAKTAT PENGHASIL EKSOPOLISAKARIDA Malik, Malik; Ariestanti, Donna M; Nurfachtiyani, Anandayu; Yanuar, Arry
Makara Journal of Science Vol. 12, No. 1
Publisher : UI Scholars Hub

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Abstract

Screening for glucosyltransferase gene (gtf) from exopolysaccahride producing lactic acid bacteria. Glucosyltransferase (GTF) is an enzyme involved in exopolysaccharide (EPS) polymer synthesis in microbes. One example of EPS that has been used in pharmaceutical and medical application is dextran. Dextran has been used in conjugated-drug delivery system as matrix. As a group of microbes producing EPS, lactic acid bacteria (LAB) have been well reported carrying sucrase genes glucosyltransferase (gtf), as well as fructosyltransferases (ftf). In an attempt to search for novel gtf genes as the aim of this study, LAB collection isolated from local sources yielded from previous study were screened performing PCR using degenerate primers DegFor and DegRev. An approximately 660 base pairs (bp) amplicons were obtained by using genomic DNAs of those LAB isolates as templates with conserved region of gtf genes catalytic domain as target. Two out of 20 LAB strains were yielded no amplicon as observed on agarose gel, while one strain exhibited non-specific amplicon DNA bands with sizes other than 660 bp. The two negative ones were isolated from soil obtained from dairy product waste field and from waste of soy sauce from previous study, while the latter was isolated from waste of soy sauce.
Co-Authors . Hayun Abdul Mun’im Ahdyani, Risa Amarila Malik Ambarsari, Christy Anandayu Nurfachtiyani Andika Andika Andry Nur Hidayat Andry Nur-Hidayat Anton Bahtiar Anton Bahtiar Arba, Muhammad Arfan Arfan Ari Wibisono Ari Wibisono Ariestanti, Donna M Arif Arrahman Arif Arrahman Asmiyenti Djaliasrin Djalil Azminah Azminah Bambang Wispriyono Bustamam, Alhadi Cahaya Azzahra Rahmadhani Chun Wu Claus, Matheus Prayoga Daryono Hadi Tjahjono Donna M. Ariestanti Donny Risnanda Herdien Dylan James Brunt Effionora Anwar Erlina, Linda Firdayani Firdayani Gilbert Fleischer Hariyanti Hariyanti Haviani Rizka Nurcahyaningtyas Hayun Hayun Hayun Hayun Hayun Herman Suryadi Hertono, Gatot Fatwanto Heru Suhartanto Ida Usman Illahi, Adha Dastu Joko Tri Wibowo, Joko Tri Joshita Djajadisastra Joshita Djajadisastra, Joshita Kusmadi Kurmardi Laila Fitria LARASATI, ANNISA LAZUARDI Leonardus Broto Sugeng Kardono Maksum Radji Malik Malik, Malik Masteria Yunovilsa Putra Maulana, Irvan Mohamad Kashuri Muhammad Hanafi Muhammad Sulaiman Zubair Munaya Azizah Nadhifatul Aslikah Nur Hasanah Nurfachtiyani, Anandayu Nursanti Nursanti Nursanti Nursanti Putra, Masteria Yunovilsa Rani Sauriasari, Rani Retnosari Andrajati Richa Mardianingrum Rizna Triana Dewi Ruslin Ruslin Ruswanto, Ruswanto Sanang Nur Safitri Septia Nurmala Setiajid, Muhammad Aditya Setiajid, Muhammad Aditya Setyanto Tri Wahyudi Silvia Surini Siwi, Inas Priasti Sutriyo Sutriyo, Sutriyo Teni Ernawati Wening Lestari Yohanes Gultom Yona Harianti Putri YUDI SRIFIANA