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LALU ZULKIFLI
STMIK AMIKOM Yogyakarta
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Effect of Adding Pectin on The Quality of Red Dragon Fruit (Hylocereus polyrhizus) Sheet Jam Zanaria; Mahrus; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6376

Abstract

Dragon fruit has a high water content and a limited shelf life. Dragon fruit has the potential to be used as a raw material for food processing, one of which is jam. Dragon fruit contains pectin which functions as a gelling agent in jam. This research aims to determine the addition of pectin with different concentrations on the quality of red dragon fruit jam sheets. This research is an experimental study using a completely randomized design (CDR) with four treatments and four replications. Data were analyzed using Analysis of Varience (ANOVA) to obtain Duncan New Multiple Range Test (DNMRT) results at level 5%. The research results showed that the effect of pectin with different concentrations provided significant differences (P<0.05) on water content, ash content, sucrose content, crude fiber content, total dissolved solids, degree of acidity (pH), and organoleptic tests. The addition of 0.75% pectin affects the chemical, physical and organoleptic properties of Red Dragon Fruit Jam. Chemical ingredients consist of water, ash, sucrose content, crude fiber content, total dissolved solids, acidity degree (pH), and physical characteristics including taste, color, aroma and texture. A total of 25 panelists liked the organoleptic properties. In conclusion, the effect of adding pectin on the quality of the best jam product is P3 (0.75% pectin addition) so it is more acceptable based on its character and organoleptic properties.
Antagonistic Test of Bacteria Producing Siderophore and Protease Enzymes from The Rhizosfer of Peanut Plants on The Growth of Pathogenic Fungus Colletotrichum gloeosporioides Nurikhsanti, Mikiyal; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6459

Abstract

This study aims to determine the inhibitory effect of bacterial isolates producing siderophores and protease enzymes on the growth of the pathogenic fungus Colletotrichum gloeosporioides. The initial stage of research begins with the isolation of pathogenic bacteria and fungi, and is followed by testing the production of siderophores and protease enzymes. Bacteria were isolated from the rhizosphere of peanut plants, while pathogenic fungi were isolated from large chili fruit infected with anthracnose disease taken from Jelantik Village, Central Lombok Regency. Characterization of isolates for siderophore production used the Arnow method, while the protease enzyme production test used SKIM Milk Agar media. Next, the inhibition test of bacterial isolates against pathogenic fungi was carried out using the dual culture method. Characterization of potential isolates was carried out by observing bacterial colony morphology, gram staining and biochemical tests. The results of the siderophore production test showed that there was one isolate capable of producing siderophores with the isolate code RKT2. Meanwhile, the protease enzyme production test showed that all bacterial isolates produced protease enzymes, where isolate RKT9 had the highest Proteolytic Index, namely 1.57. The two isolates showed different inhibitory test results, namely isolate RKT2 had high inhibition, while RKT9 had low inhibition. The results of the research showed that a bacterial isolate (RKT2) from the rhizosphere of peanut plants was able to inhibit the growth of the pathogenic fungus Colletotrichum gloeosporioides in the high category.
Influence of IAA-Producing Bacteria and Phosphate Solubilizers from The Rhizosphere of Serbania grandiflora (L.) Pers on The in vitro Germination of Vigna radiata (L.) Arianti, Septia; Zulkifli, Lalu; Mertha, I Gde; Rasmi, Dewa Ayu Citra
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6460

Abstract

This study aims to determine the effect of IAA-producing bacteria and phosphate solvents from the rhizosphere of Serbania grandiflora (L.) Pers on the germination of Vigna radiata (L.). This research began by isolating the rhizosphere bacterium Serbania grandiflora (L.) Pers, which was then characterized by cell morphology, grammatical and morphological characteristics. The ability of bacteria to dissolve phosphate was evaluated qualitatively by observing the presence of clear zones in Pikovskaya media, and quantitatively with a spectrophotometer. The isolate's ability to produce IAA was tested quantitatively using Salkowski reagent, and quantitatively using a spectrophotometer. For the effect of isolates on the growth of green beans, a completely randomized design (CRD) research design was used, with 4 treatments, namely P0 (sterile distilled water); P1 (IAA-producing bacterial culture); P2 (phosphate solubilizing bacterial culture); P3 (IAA producer and phosphate solubilizer). Vigna radiata L. seedling growth test was carried out in vitro on Murphy's medium. The research data were analyzed using the one-way ANOVA test. The results showed that 5 isolates had the ability to dissolve phosphate, with a phosphate solubility index ranging from 1.70-2.14, where isolate RT7 showed the highest value. The isolate's ability to produce IAA at concentrations ranged from 7.1 - 62.7 ppm, with the highest value shown by isolate RT6. The results showed that treatment with IAA-producing bacterial isolates plus phosphate solubilization properties showed a significantly different effect on Vigna radiata L. plant height compared to other treatments.
Molecular and Phylogenetic Character Analysis of Tuna (Auxis rochei) Based on the CO1 Gene in the Waters of West Lombok Darmawan, Rizky Ferdyanto; H. Mahrus; Syamsul Bahri; Zulkifli, Lalu
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6768

Abstract

Tuna Fish is a type of tuna with a medium body size and fair wide distribution in Indo-Pacific waters. The number of Tuna Fish species that have morphological similarities can cause misidentification. This study aims to determine the genetic and phylogenetic diversity between variations of Tuna Fish species in the West Lombok Waters. This research is an exploratory study with the object of research being a dataset of ingroup sequences of tuna and outgroup sequences from the same family. The amplification of fish samples used DNA primers targeting the CO1 gene, which resulted in a sequence length of approximately 650 bp. Sequence data were analyzed using MEGA XI software known from phylogenetic tree analysis shows that Tuna Fish from the waters of the West Lombok are closely related to Tuna Fish from the South China Sea and have a genetic distance of less than 3%.
Antagonistic Test of Endophytic Bacteria of Chili Pepper Roots Producing Siderophores and Hydrolase Enzymes against Plant Pathogenic Bacteria Ralstonia Solanacearum Hidayati, Siti Nur Isnaini; Zulkifli, Lalu; Sedijani, Prapti; Rasmi, Dewa Ayu Citra
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7966

Abstract

This study aims to test the ability of endophytic bacterial isolates of cayenne pepper roots (Capsicum frutescens) to produce siderophore compounds and protease enzymes and to test the antagonistic activity of endophytic bacterial isolates in controlling the growth of plant pathogenic bacteria (Ralstonia solanacearum). The stages in this study are isolation of endophytic bacteria from cayenne pepper roots, siderophore production test, protease enzyme activity test, and biochemical and physiological characterization of potential endophytic bacteria, antagonistic test of endophytic bacteria against the growth of Ralstonia solanacearum bacteria. Endophytic bacteria were isolated from cayenne pepper plant roots taken from Selong, East Lombok. Characterization of siderophore-producing isolates used the Arnow test method. The activity of bacterial protease enzymes was tested qualitatively on Skim Milk Agar solid media. The antagonistic activity test of endophytic bacteria against pathogenic bacteria was carried out using the Kirby-Bauer diffusion method. Characterization of endophytic bacteria was carried out by observing colony morphology, Gram staining, and biochemical tests. There were 3 isolates that were able to produce siderophores (catecholate type) with codes EC5, EC12, and EC15. Six isolates showed protease enzyme activity with codes EC3, EC4, EC5, EC7, EC11, and EC15, with the highest Proteolytic Index (2.02) shown by isolate EC4. There were 3 isolates that were able to inhibit the growth of Ralstonia solanacearum (EC4, EC5, and EC15), with the highest inhibitory power shown by isolate EC15 (3.67 mm). The results of the study indicate that endophytic bacteria from cayenne pepper plant roots have the potential as biopesticides in the future.
Exploration of P-Solubilizing and IAA-producing Rhizobacteria from Saline Environments: Their Effects on Vigna radiata Growth-promotion Utami, Ni Wayan Anggun Diah; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7968

Abstract

Rhizobacteria that are able to dissolve P and produce IAA can be used as component of biofertilizers to help increase plant growth, which can be a more environmentally friendly fertilizer alternative compared to chemical fertilizers. Rhizobacteria originating from sub-optimal environments have the potential to overcome environmental stress such as osmotic stress. To obtain potential isolates that can be used as biofertilizer, exploration of the rhizosphere of the sentigi (Pemphis acidula) has been carried out from the coast of Gili Sulat, Lombok. The method for isolating and selecting IAA producing bacteria was carried out using the colorimetric test method, while for the P solubilization ability, it was carried out by growing rhizobacteria on Pikovskaya media. Isolate PA6 with the highest IAA producing ability (21 ppm) and PL4 with the highest P solubilizing ability (20.9 ppm), were tested for their effect on the growth-promotion of Vigna radiata cultured on Murphy agar media by measuring the parameters of plant height, root length, fresh weight, and plant dry weight. Based on the One Way ANOVA statistical analysis with a p value ≤ 0.05, it showed that the inoculation of IAA-producing and P solvent rhizobacteria was proven to be able to have a significant effect with significantly different results on increasing plant dry weight. In the future, the isolate obtained can be developed into a biofertilizer component that can be applied in saline and dryland agriculture.
Exploration and Inventory of Epiphytic Plants in the Forest of Lemor Botanical Gardens, Suela District, Lombok Island Nabila, Elva Elvina; Zulkifli, Lalu; Raksun, Ahmad; Mertha, I Gde
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8754

Abstract

Various types of plants are found in Lemor Botanical Garden Forest (located in Suela Village, East Lombok Regency), one of which is epiphytic plants. Epiphytic plant are plants that live attached to other plants. This study aims to determine the types of epiphytic in the Lemor Botanical Garden Forest and the most dominating epiphytic species based on the environmental characteristics of the Lemor Botanical Garden Forest. This research is a descriptive research. Data collection was carried out using the cruisi method from October to November 2024. Data were analyzed qualitatively. The results showed that in the Lemor Botanical Garden Forest, 15 species of epiphytic were found consisting of 8 families and 797 individuals.  The types of epiphytic found include: Grosourdya appendiculata, Nephrolepis exaltata, Microlepia todayensis, Goniophlebium verrucosum, Goniophlebium persicifolium, Drynaria quercifolia, Drynaria sparsisora, Pyrrosia eleagnifolia, Pyrrosia longifolia, Microsorum punctatum, Cryptogonium phyllogonioides, Thuidium glaucinoides, Rhaphidophora pinnata, Syngonium podophyllum, and Piper betle. The most dominating epiphytic species based on the environmental characteristics of Lemor Botanical Garden Forest are epiphytic found growing in the watershed many as 13 species, 3 of which are: Drynaria quercifolia, Microsorum punctatum, and Rhaphidophora pinnata, with a percentage of 37%. The most dominant species found was Drynaria quercifolia from the Polypodiaceae family with 148 individuals.
Sosialisasi Keamanan Pangan Produk Rekayasa Genetika (PRG) Kepada Siswa MAN 2 Mataram Mahrus; Idrus, Agil Al; Zulkifli, Lalu; Syauki MS, Lalu
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.6260

Abstract

Kehadiran produk rekayasa genetika di bidang pertanian bertujuan untuk mengatasi kekurangan pangan manusia, meningkatkan produktivitas tanaman, ketahanan terhadap tekanan lingkungan yang ekstrim, dan lain sebagainya. Upaya peningkatan produksi pangan terus dilakukan seiring dengan peningkatan kebutuhan pangan karena pertumbuhan penduduk yang semakin tinggi dari tahun ke tahun. Dalam perjalanannya produk rekayasa genetika bukannya tanpa kendala karena ternyata banyak pro dan kontra. Di satu sisi, perkembangan ilmu pengetahuan di bidang rekayasa genetika tanaman sangat pesat. Kegiatan pengabdian pada masyarakat di MAN 2 Mataram ini bertujuan untuk mesosialisasikan keamanan pangan produk rekayasa genetika. Diharapkan kepada para Siswa Kelas XI MAN 2 Mataram sebagai target sasaran kegiatan dapat menyebarluaskan informasi yang didapatkan kepada orang lain seperti: saudara, keluarga dan masyarakat luas, sehingga rasa aman mengonsumsi produk pangan hasil rekayasa genetika terjamin. Bentuk kegiatan yang dilakukan adalah pembelajaran di kelas dengan menggunakan metode ceramah, diskusi dan tanya jawab. Materi pembelajaran yang diberikan meliputi: Konsep dan Pengertian Rekayasa Genetika, Teknik Rekayasa Genetika, Produk Rekayasa Genetika (PRG), dan Peraturan Pemerintah tentang Penggunaan PRG. Program Pengabdian Kepada Masyarakat di MAN 2 Mataram telah dilaksanakan dengan baik. Hal ini terlihat dari partisipasi dan berbagai pertanyaan yang dilontarkan siswa kelas XI IPA pada saat diskusi dan tanya jawab. Tim memberikan kunci-kunci utama yang digunakan dalam mengenali PRG. Tim Pengabdian Kepada Masyarakat berhasil memberikan pemahaman yang komprehensif terhadap keamanan PRG dan menyebarluaskannya kepada Masyarakat sesuai dengan rambu-rambu yang tertuang dalam Peraturan Pemerintah No. 21 Tahun 2005 tentang Keamanan Pangan Produk Rekayasa Genetika.
Pengenalan Ekosistem Mangrove untuk Penguatan Literasi Kebaharian Pada Siswa SMPN 2 Pujut Lombok Tengah Japa, Lalu; Syukur, Abdul; Karnan; Zulkifli, Lalu; Winata, I Gede Darma; Dianam
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.6550

Abstract

Wilayah pesisir dan lautan sangat penting artinya bagi masyarakat. Sebagai akibat dari aktivitas dimaksud, tidak bisa dipungkiri, bahwa kondisi sumber daya alam pesisir dan laut mengalami penurunan. Penurunan kondisi sumber daya strategis ini disebabkan oleh dua faktor, alami dan manusia (antropogenik). Dari kedua faktor yang menyebabkan kerusakan lingkungan bahari ini, hanya faktor antropogenik yang bisa dikendalikan dalam upaya meminimalisir kerusakan yang terjadi. Salah satu upaya pengendalian dimaksud adalah melalui jalur pendidikan formal di sekolah yaitu penanaman cinta bahari sejak dini agar sumber daya kebaharian dapat dilestarikan dan pemanfaatannya bisa berkelanjutan. Kegiatan pengabdian ini bertujuan untuk: meningkatkan literasi kebaharian siswa SMPN 2 Pujut sebagai salah satu sekolah yang berada di kawasan KEK Mandalika melalui pengenalan ekosistem mangrove. Untuk maksud di atas, maka siswa SMPN 2 Pujut dilibatkan secara aktif menelaah dan berdiskusi terkait materi tentang ekosistem mangrove bedasarkan buku ekowisata ekosistem mangrove. Dalam jangka panjang, kegiatan pengabdian ini diharapakan bisa menghasilkan suatu model pembelajaran terintegrasi guna meningkatkan literasi kebaharian siswa dimana pengetahuan kebaharian akan dimasukkan sebagai bagian dari bahan materi yang diajarkan (silabaus) pembelajaran di sekolah. Dalam jangka pendek, diharapkan bisa menghasilkan profile literasi kebaharian siswa di KEK Mandalika sebagai bahan untuk menyusun bahan ajar yang terintegrasi guna meningkatkan literasi kebaharian siswa sebagaimana amanat dan tuntutan kurikulum merdeka yang sudah mulai dilaksanakan termasuk di SMPN 2 Pujut Lombok Tengah.
Aplikasi Kompos Kotoran Kambing pada Budidaya Tanaman Terung di Dusun Mertak Umbak Kecamatan Praya Lombok Tengah Raksun, Ahmad; Karnan; Zulkifli, Lalu; Santoso, Didik; Japa, Lalu
Jurnal Pengabdian Magister Pendidikan IPA Vol 7 No 2 (2024): April-Juni
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v7i2.8019

Abstract

Kegiatan pengabdian kepada masyarakat tetang aplikasi kompos kotoran kambing pada budidaya tanaman terung telah dilakukan di Dusun Mertak Umbak Kecamatan Praya Kabupaten Lombok Tengah. Mitra kegiatan pengabdian adalah 10 orang petani yang berdomisili di RT 01 Dusun Mertak Umbak Desa Mertak Tombok. Ada 2 permasalahan yang dihadapi mitra, yaitu: mitra belum memiliki pengetahuan tetang cara pengolahan sampah organik menjadi suatu produk yang bermanfaat. Permasalahan kedua adalah tingginya biaya usaha pertanian yang disebabkan oleh tingginya jumlah pupuk kimia yang diperlukan dan harus dibeli oleh mitra dengan harga yang mahal. Penyelesaian masalah pertama dilakukan dengan mengolah sampah organik menjadi kompos dan penyelesaian masalah yang kedua dilakukan dengan melakukan pemupukan tanaman menggunakan kompos. Ujicoba aplikasi kompos dilakukan pada tanaman terung hijau. Pada pelaksanaan kegiatan pengabdian kepada masyarakat ini, mitra terlibat secara aktif mulai dari awal sampai dengan akhir kegiatan. Setelah berakhirnya kegiatan ini mitra dapat melakukan pengolahan sampah organik menjadi kompos serta mendapatkan pengetahuan dan keterampilan dalam aplikasi kompos pada lahan pertanian.
Co-Authors Abdul Syukur Agil Al Idrus Agil Al Idrus, Agil Al Agus Ramdani Ahmad Raksun, Ahmad Alhafizin, M. Apriana, Mila Arianti, Septia Ayu Citra Rasmi, Dewa Baiq Sri Handayani Cahyadi Wirajagat, Gde Darmawan, Rizky Ferdyanto Dianam Didik Santoso Dwi Soelistya Dyah Jekti Fadilla Yuliana, Baiq Fitriani, Iwan H. Mahrus Handayani, Baiq. Sri Hariadi, Ismawan Hidayati, Siti Nur Isnaini I Gde Mertha I Wayan Merta I Wayan Mertha Ilhamdi, M. Liwa Ilhamdi, Moh Liwa Ilhamdi, Mohammad Liwa Karnan Karnan Kodri Madang, Kodri Lalu Japa, Lalu Mahrus Mahrus Mahrus, Mahrus Muhammad Zaki Musaddat, Syaiful Nabila, Elva Elvina Ni Luh Putu Pebri Artayani . NINING INTAN TOHARAH, NINING INTAN Nurikhsanti, Mikiyal Ovie Arista Paspania, Karima PRAPTI SEDIJANI, PRAPTI Priyo Hartanto Putra, Hendra Susana Raden Mohamad Herdian Bhakti Rasmi, Dewa Ayu Citra Rizki, Risa Mar’ussifa Robi'atul Adawiyah, Marosa Rusyda, Mentari Tatsbita Sakaroni, Rubiyatna Sapril, Dian Islamiaty Sari, Neti Kumala Sofian, M. Syamsul Bahri Syauki MS, Lalu Syauki, Lalu UMAMI, RIZAL Utami, Febrisa Indri Utami, Ni Wayan Anggun Diah Wardana, Lalu Ali Winata, I Gede Darma Wirajagat , Cahyadi Zanaria