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All Journal HAYATI Journal of Biosciences VALENSI Bionatura Microbiology Indonesia Majalah Kedokteran Bandung Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Health Notions Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Jurnal Kimia Terapan Indonesia Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry PENDIPA Journal of Science Education Molekul: Jurnal Ilmiah Kimia Jurnal Ilmiah Farmako Bahari
Shabarni Gaffar
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health

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Journal : Bionatura

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PHYLOGENETICS ANALYSIS OF MARINE AND COASTAL SPECIES USING 18S rRNA SEQUENCE Shabarni Gaffar -; Linawati Hardjito -; Endang Srieatimah -
Bionatura Vol 11, No 2 (2009): Bionatura Juli 2009
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Phylogenetics analysis using 18S rRNA sequence was done on some marine and coastal organisms that have been used as traditional medicine in Indonesia. Proper and accurate identification of coastal and marine organisms producing bioactive compound is important to support drug discovery. Ribosomal RNA has been described as one of most useful and most used for molecular chronometer. In this experiment a part of 18S rRNA gen was amplified using PCR employing 300F and 1400R primers. PCR product then was cloned using pGEM-T vector (Promega) and transformed into Eschericia coli. The recombinant Eschericia coli were sequenced by applying universal primer and internal primer. The alignment of sequenced product and phylogenetic analysis indicated that sample 1 closely related to Chaetomorpha crassa, sample 2 closely related to Ptilophora pinnatifida, sample 3 closely related to Flagellaria indica, and sample 4 closely related to Wallemia ichthyophaga. Keywords: phylogenetic analysis, 18S rRNA sequence.
Co-Authors Aathirah, A Sayyidatina Abu Bakar M.I. Syihab Ade Rizki Ridwan Firdaus Aga Adi Masyhuri Agus Safari Al Arofatus Naini Albayyinah, Dyandra Hera Amalia, Riezki Anni Anggraeni, Anni Annisa Ilma Naviardianti Annisa Ilma Naviardianti Ari Hardianto Arum Kurnia Sari Bacthi Alisjahbana Bayu Shiddiq Widhi Pratama Dani Permana Darwati Darwati Desi Harneti Putri Huspa DESSY NATALIA Diana P Rahmawati Egista Istioka Fazrin Ekawardani, Savira Endang Srieatimah - Erina Hilmayanti Ersanda Hafiz Euis Julaeha Euis Julaeha Fajriyah, Maulida Ghina Nur Fadhilah Ghina Uli Felicia Tambunan Hafiz, Ersanda Hesti Lina Wiraswati Iman Permana Maksum Iqbal Wahyu Mustaqim Irkham Irkham KHOMAINI HASAN Kindi Farabi Korry Novitriani, Korry Lia Faridah LINAWATI HARDJITO Lubis, Rubianto A. M. Lutfi Firdaus Ma'ruf, Ilma Fauziah Masyhuri, Aga Adi Mohamad Nurul Azmi Muhammad Hilman Daniswara Muhammad Yudha Nugraha Muhammad Yusuf Murniaty Simorangkir Nayla Haraswati Nurlelasari Nurlelasari Nurmalasari, Ratna Nurul Auliany O Suprijana Oo Suprijana Permadi, Nandang Pertiwi, Wulan Purba Upay Purnama Purnama PUTRI, RAFIKA Putri, Rafika Nanda R. Ukun M.S. Soedjanaatmadja Rani Maharani Rima Melati Rini Surbakti Riza Apriani Riza Apriani Riza Apriani Rubianto A. Lubis Rudi Hartono Rustaman Rustaman Saadah D. Rachman Safri Ishmayana Safrl Ismayana Santhy Wyantuti Sari Syahruni Saronom Silaban SARONOM SILABAN Savira Ekawardhani Shabrinna, Hanif Soetijoso Soemitro Soetijoso Soemitro SOETIJOSO SOEMITRO Sofa Fajriah Sofyan Multazam N Aji Supriyadi, Isma Yustifania SUTARYA ENUS Sutarya Enus Syifa Al Fauziah Rahmani Tambunan, Ghina Uli Felicia Tati Herlina Tati Herlina Toto Subroto Tri Mayanti Triana N Meirina Umi Baroroh Unang Supratman Upay, Purba Vicki Nishinarizki Wulan Pertiwi Yeni W Hartati Yeni Wahyuni Hartati Yenni Wahyuni Hartati, Yenni Wahyuni Yohan Yohan Yohan Yohan Yoshihito Shiono