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Growth Analysis and Identification of Viral Diseases (WSSV, IHHNV, IMNV) and AHPND in White Shrimp (Litopenaeus vannamei) Cultivation using Millennial Shrimp Farming (MSF) System Zulkisam Pramudia; Abd Rahem Faqih; Agus Fery Setiawan; M. Amenan; Andi Kurniawan
Indonesian Journal of Environment and Sustainable Development Vol. 13 No. 2 (2022): Jurnal Pembangunan dan Alam Lestari
Publisher : Postgraduate School Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2022.013.02.04

Abstract

The aquaculture sector continues to be developed to support sustainable development in Indonesia. One of the efforts made to develop sustainable aquaculture in the fisheries sector is the application of sustainable technological innovations to increase aquaculture productivity. The Millennial Shrimp Farming (MSF) system for white shrimp cultivation has developed to achieve increased production that can be carried out on limited land and relatively small business capital. However, although the MSF system has been widely carried out, studies reporting on monitoring shrimp growth and viral diseases in the MSF system in Indonesia have not been widely reported. This study aims to monitor the growth of white shrimp and the presence of viral diseases during white shrimp culture using the MSF system in Indonesia. This study was conducted in 3 MSF ponds by calculating growth and identifying viral diseases, namely White Spot Syndrome Virus (WSSV), Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV), Infectious Myo Necrosis Virus (IMNV), and Acute hepatopancreatic necrosis disease (AHPND) with using the Polymerase Chain Reaction (PCR) method. Based on the results, the growth parameters observed on the Day of Culture (DOC) 30, 37, 44, 51, 58, 65, and 72 in the three ponds had an average weight above the standard weight compared to the Indonesian Nasional Standard (SNI 01-7246-2016) regarding the production of white shrimp in ponds with intensive technology. Meanwhile, the results of identifying viral diseases (WSSV, IHHNV, IMNV) and AHPND on DOC 1, 7, 42, 66, and 72 all showed negative results. The results above indicate that the cultivation of white shrimp with the MSF method showed optimum results for growth parameters, and no viral disease was identified during cultivation. Keywords: Litopenaeus vannamei, MSF, Virus, WSSV, IHHNV, IMNV, and AHPND
Endemic Fish Conservation: Utilization of Cryopreservation Technology with Fructose in Red Bader Fish (Puntius Bramoides) Sperm Danang Yonarta; Abd Rahem Faqih
Journal of Aquaculture and Fish Health Vol. 12 No. 3 (2023): JAFH Vol. 12 No 3 September 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i3.39212

Abstract

Cryopreservation in red bader fish is needed for conservation and development of gamete cell storage. It is a chemical compound that can prevent cell or tissue damage due to freezing. In addition, dimethyl sulfoxide can penetrate cells quickly during equilibration. This research aimed to study reproductive biology and analyze the fructose ability as a extender in the Red Bader fish sperm cryopreservation process. The cryopreservation process was conducted at the Artificial Insemination Center, Singosari. The test fish were obtained from the Freshwater Cultivation Development Center, Umbulan then they were reared for 2 months to get the level of gonad maturity. This study was designed using a completely randomized design (CRD) which consisted of three treatments and three replications. The treatment given was the use of fructose extenders with different percentages i.e., 0.2%, 0.4%, and 0.6%. The results showed that the sperm characteristics of the red bader fish (Puntius bramoides) had a volume of 3.18 mL, a pH value of 7.39, a milky white sperm color, a sperm concentration of 3.5x109 cells/mL, a motility value of 81.67%, and a viability of 85 %. The best type of fructose extender with 0,6% dimethylsulfoxide concentration has a motility value of 38,33% post-cryopservation and 36,67% post-cryopreservation viability. The type of extender affects the sperm quality of angry bader fish during the cryopreservation process, the type of extender obtained was fructose with the best concentration of 0,6% dimethylsulfoxide with the highest motility and viability values.
Dynamics of Water Quality for Vannamei Shrimp Cultivation in Intensive Ponds in Coastal Areas Rizky Kusma Pratiwi; Mohammad Mahmudi; Abdul Rahem Faqih; Diana Arfiati
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4322

Abstract

Cultivators increase production using intensive cultivation which applies stocking densities and feeding in high quantities, which triggers a decrease in water quality, both cultivation water and cultivation wastewater because it contains high levels of organic matter. Changes in water quality have an impact on plankton community structure. This research aims to determine the dynamics of the physical, chemical, and biological quality of cultivation media water and the disposal of vaname shrimp cultivation waste in Ujungpangkah District, Gresik Regency. The research was conducted in January 2023 using a survey method. Water and plankton samples were taken from reservoirs, ponds, and pond waste disposal sites. Sampling was carried out in vaname shrimp cultivation ponds in the Coastal Area of ​​Ujungpangkah District, Gresik Regency. The physical and chemical quality of water has a pattern of changes up and down but is relatively the same and stable every week. All of these water quality parameters show a pattern of change that is quite good for the growth of vaname shrimp, but in waste disposal, the parameters for nitrate, phosphate, ammonia, and TOM are above the quality standards. The highest abundance of plankton is in waste dumps, with the highest phytoplankton coming from the Bacillariophyta phylum (49%), while zooplankton comes from the Rotifera phylum (54%). Regular monitoring of water quality in ponds and waste disposal sites is required so that it remains stable and safe for the environment.
Effect of Dosages and Temperatures on Simalungun Red Ginger (Zingiber officinale var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (Danio rerio) as a Model Fish Eka Fitria Febriani; Maheno Sri Widodo; Abdul Rahem Faqih
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.38271

Abstract

Highlight Research Successive administration of Ginger ethanol extract to male individuals was shown to be able to produce a significant increase in sperm count, motility, viability and morphology Ethanol extract of ginger has androgenic activity which is proven to be able to increase the concentration of testosterone in serum, which functions to control the process of spermatogenesis, maintain Sertoli cells and play a role in determining the quality of spermatozoa in male organisms Giving ginger ethanol extract for 30 consecutive days to male individuals showed significant results so that ginger extract was thought to have the potential to improve male characteristics Ginger extract is potentially useful in improving healthy sperm characteristics and management of male infertility especially in those with low sperm counts   Abstract Ornamental fish are known to be easily cultivated in aquarium or pond. Many people prefer male (MF) over female fish (FF) due to their aesthetic value and productivity which lead to better pricing. The aim of this study was to determine the effect of Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG) on masculinization. This study used dipping method which was carried out using Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG). The temperature treatments used in this study were 28°C and 32°C. For this research, an experimental study with a Completely Randomized Factorial Design (CRFD) method that had two independent variables such as different dosages and temperatures was used. From five dosages (0 mg/L; 5 mg/L; 10 mg/L; 15 mg/L; 20 mg/L) and three temperature treatments (ambient temperature (about 25°C; 28°C; 32°C), it was found that the highest male percentage of primary sexuality (88.33%), secondary sexuality (85%), and highest total testosterone levels (TL) (1.986 ng/L) were at the treatments with the highest dosages and temperature, while for the highest survival rate were at almost in all B treatments (5 mg/L) and b levels (28°C), in which the survival rate was 100%. For all of these results above, the results were significant.
GONAD MATURITY LEVELS OF LEMPUK FISH (Gobiopterus sp) IN RANU GRATI, PASURUAN, EAST JAVA Anitasari, Septi; Yanuhar, Uun; Faqih, Abd Rahem; Sanoesi, Ellana; Susilowati, Surya Rachman; Sansi, Delfina Husniya
Chanos Chanos Vol 22, No 1 (2024): CHANOS CHANOS
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/chanos.v22i1.14358

Abstract

Pasuruan Regency in East Java Province boasts a natural lake, Ranu Grati, formed by volcanic activity, covering about 50 hectares and home to endemic species like the Lempuk fish (Gobiopterus sp.). These fish play a crucial role in Ranu Grati's trophic structure, serving as primary consumers and key planktonic organisms supporting higher trophic levels. The Lempuk fish population faces sustainability challenges due to high demand for this traditional food, leading to overexploitation and decreased reproductive capabilities. Understanding the reproductive biology, particularly gonad maturity levels, is vital for effective resource management. This study, conducted from June to August 2023, aimed to investigate the reproductive biology and gonad maturity levels of Lempuk fish in Ranu Grati. Analysis revealed a male-biased sex ratio, with significant fluctuations in gonad maturity levels across months. In June, the sex ratio was 1.05, in July 1.17, and in August 1.39, indicating a higher population of males. Histological observations showed asynchronous egg development, likely influenced by environmental factors and the fish's reproductive strategy 
KAJIAN MORFOMETRIK DAN NISBAH JENIS KELAMIN IKAN LEMPUK DI RANU GRATI, KABUPATEN PASURUAN, JAWA TIMUR Anitasari, Septi; Faqih, Abd Rahem; Kusuma, Wahyu Endra; Yuniarti, Ating
Jurnal Harpodon Borneo VOLUME 14 NO. 1 APRIL 2021
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v14i1.1898

Abstract

Ikan Lempuk merupakan salah satu komoditas perikanan yang menjadi salah satu sumber kebutuhan protein hewani masyarakat serta daya tarik wisatawan yang berkunjung ke Ranu Grati Pasuruan Jawa Timur Indonesia. Ikan Lempuk mudah ditemukan di Ranu Grati dan tidak ditemukan di tempat lain. Hasil yang diperoleh dari penelitian ini adalah ikan Lempuk Ranu Grati termasuk dalam genus Gobiopterus sp. Morfologi ikan ini dapat dikenali dari warna tubuhnya yang transparan dimana organ dalam seperti jantung, ginjal, kantung renang, pembuluh darah dan tulang belakang dapat dilihat dari luar tubuh. Ikan ini memiliki panjang rata-rata 2,43 cm, berat rata-rata 0,1223 gram. Bentuk badan fusiform, letak mulut sub terminal superior, bentuk sirip ekor truncate dengan tipe ekor diphycercal. Ikan ini memiliki gigi pada kedua rahang atau biasa disebut letak gigi vomer dan memiliki bentuk gigi canine, terdapat dua jenis bentuk ekor yaitu tipe Rounded dan Truncate.. Nisbah jenis kelamin lebih dominan pada jantan daripada betina, dengan perbandingan  6,2: 3,8.
Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish Hutagalung, Rizal Akbar; Widodo, Maheno Sri; Faqih, Abdul Rahem
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2996.134 KB) | DOI: 10.19027/jai.14.24-29

Abstract

ABSTRACT Snakehead fish (Channa striata) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish  ABSTRAK Ikan gabus (Channa striata) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%, Kata kunci: PMSG, IGS, IHS, ikan gabus 
Ovulation time and ovulated eggs count of sneakehead Channa striata induced by prostaglandin 2α with different doses Jamlaay, Frits; Widodo, Maheno Sri; Faqih, Abd. Rahem
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3067.389 KB) | DOI: 10.19027/jai.15.89-92

Abstract

ABSTRACT Snakehead fish Channa striata is one of freshwater commodity which has high economic value. Nowadays, snakehead fish stock mostly comes from wild capture. Artificial spawning with hormonal induction is usually used to improve broodstock efficiency, as well as the quality and quantity of fish seed production. Purpose of this study was to determine the effectiveness of prostaglandin 2α (PGF2α) hormone on ovulation time and count of ovulated eggs. This experiment used 30–40 cm broodstock with 600–900 g body weight. Fishes were acclimated for one week in a container and then injected with different doses of PGF2α, namely: control without PGF2α (P1), 0.5 mL/kg (P2); 0.7mL/kg (P3); and 0.9 mL/kg (P4). Each treatment consisted of three fishes as replication. Ovulation time was observed until 72 hours post injection. The results showed that PGF2α injected broodstoks ovulated faster (9.17–12.24 hours post injection) than control P1 (22.67 hours), while among PGF2α injected fishes were the same. Furthermore, count of ovulated eggs from PGF2α induced broodstocks were higher than control P1, and the highest (2,860 eggs) was obtained in treatment 0.9 mL/kg. Thus, PGF2α at dose of 0.9 mL/kg can be used to induce eggs ovulation of snakehead fish and to increase count of ovulated eggs. Keywords: PGF2α, ovulation time, ovulated eggs count, Channa striata  ABSTRAK Ikan gabus Channa striata merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini, pemenuhan kebutuhan hanya bergantung pada hasil penangkapan di alam. Cara pemijahan buatan dengan induksi hormon biasanya digunakan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan yang dihasilkan. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon prostaglandin 2α (PGF2α) terhadap waktu ovulasi dan jumlah telur ikan gabus yang diovulasikan. Penelitian ini menggunakan induk ikan gabus berukuran 30–40 cm dengan bobot tubuh 600–900 g. Ikan diadaptasikan di wadah penelitian selama seminggu, kemudian diinjeksi hormon PGF2α dengan perlakuan hormon dengan dosis berbeda, yaitu: kontrol tanpa hormon (P1), 0,5 mL/kg (P2); 0,7 mL/kg (P3); dan 0,9 mL/kg (P4). Setiap perlakuan terdiri atas tiga ekor induk sebagai ulangan. Waktu ovulasi diamati sampai dengan 72 jam pascasuntik. Hasil penelitian menunjukkan bahwa induk yang disuntik PGF2α memijah lebih cepat (9,17–12,24 jam pascasuntik) daripada kontrol (22,67 jam), sedangkan antarperlakuan hormon PGF2α tidak berbeda. Selanjutnya, jumlah telur diovulasi dari induk ikan gabus yang disuntik PGF2α berjumlah lebih banyak daripada kontrol. Hasil tertinggi (2.860 butir) diperoleh dari perlakuan 0,9 mL/kg.  Dengan demikian, hormon PGF2α dosis 0,9 mL/kg dapat digunakan untuk mempercepat waktu ovulasi ikan gabus, dan meningkatkan jumlah telur yang diovulasikan. Kata kunci: PGF2α, waktu ovulasi, jumlah telur diovulasi, Channa striata 
Aplikasi Bakteri Probiotik Bacillus Spp. terhadap Pembentukan Biofilm dan Kualitas Air: Studi Eksperimental pada Media Kultur Budidaya Udang Vaname: Application of Probiotic Bacteria Bacillus Spp. on Biofilm Formation and Water Quality: an Experimental Study on Vaname Shrimp Cultury Media Moehammad, Khibar Syiar; Kurniawan , Andi; Faqih, Abd. Rahem
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 1 (2025): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.01.2

Abstract

Penelitian ini bertujuan untuk mengeksplorasi pengaruh aplikasi probiotik Bacillus spp. terhadap pembentukan biofilm dan kualitas air pada media budidaya udang vaname (Litopenaeus vannamei). Penelitian dilakukan secara eksperimental menggunakan akuarium berkapasitas 12 liter yang dilengkapi substrat HDPE sebagai media pembentukan biofilm. Parameter kualitas air yang diamati meliputi suhu, pH, oksigen terlarut (DO), salinitas, dan amonia (NH₃). Pengukuran dilakukan secara berkala selama 168 jam. Hasil penelitian menunjukkan bahwa aplikasi probiotik Bacillus spp. secara signifikan memengaruhi penurunan kadar amonia dan peningkatan ketebalan biofilm. Suhu dan pH berada dalam kisaran optimal, yaitu 28–30°C dan 7,5–8,5, yang mendukung pertumbuhan udang vaname dan aktivitas probiotik. Penurunan kadar amonia terlihat sangat signifikan, dari kondisi awal 3,9 mg/L menjadi <1 mg/L pada perlakuan probiotik. Pembentukan biofilm yang signifikan pada substrat HDPE menunjukkan bahwa probiotik mendukung terciptanya habitat mikroorganisme yang stabil, sehingga efektif dalam proses degradasi senyawa toksik seperti amonia. Pembentukan biofilm yang stabil dan Bacillus spp. mendukung proses bioremediasi serta menjaga kualitas air, sehingga menciptakan lingkungan yang optimal untuk pertumbuhan dan produktivitas udang vaname. Stabilitas biofilm ini juga berperan dalam menjaga keseimbangan ekosistem mikroba pada media budidaya. Penelitian ini menegaskan bahwa aplikasi probiotik Bacillus spp. memiliki potensi dalam meningkatkan kualitas air dan mendukung pengelolaan limbah seperti amonia, dalam sistem budidaya udang vaname. Temuan ini menunjukkan bahwa probiotik Bacillus spp. berpotensi dalam mendukung produktivitas budidaya.   This study aims to explore the effect of probiotic Bacillus spp. application on biofilm formation and water quality in vaname shrimp (Litopenaeus vannamei) culture media. The study was conducted experimentally using a 12-liter aquarium equipped with HDPE substrate as a medium for biofilm formation. Water quality parameters observed included temperature, pH, dissolved oxygen (DO), salinity, and ammonia (NH₃). Measurements were taken periodically for 168 hours. The results showed that the application of probiotic Bacillus spp. significantly affected the decrease in ammonia levels and the increase in biofilm thickness. Temperature and pH were within the optimal range of 28-30°C and 7.5-8.5, which favored vaname shrimp growth and probiotic activity. A significant decrease in ammonia levels was observed, from the initial condition of 3.9 mg/L to <1 mg/L in the probiotic treatment. Significant biofilm formation on HDPE substrate indicates that probiotics support the creation of a stable microorganism habitat, making it effective in the degradation process of toxic compounds such as ammonia. Stable biofilm formation and Bacillus spp. support the bioremediation process and maintain water quality, creating an optimal environment for vaname shrimp growth and productivity. This biofilm stability also plays a role in maintaining the balance of the microbial ecosystem in the culture medium. This study confirms that the application of probiotic Bacillus spp. has a positive effect on the growth of vaname shrimp.
THE EFFECT OF TEMPERATURE MANIPULATION ON THE GROWTH OF AXOLOTL (Ambystoma mexicanum) Afnan, Naufal Izzudin; Faqih, Abd. Rahem; Dailami, Muhammad
Jurnal Perikanan Unram Vol 15 No 2 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i2.1463

Abstract

Axolotl (Ambystoma mexicanum) merupakan hewan unik yang populasinya sudah terancam punah. Axolotl merupakan yang unik, salah satu keunikannya adalah dapat meregenerasi hampir seluruh bagian tubuhnya. Di Indonesia sendiri masih sedikit sekali yang membudidayakan hewan satu ini. Di Indonesia kegiatan budidaya axolotl banyak menemui hambatan terutama karena faktor lingkungan yang sangat berbeda dengan habitat aslinya. Salah satu kendala utama untuk membudidayakan axolotl adalah suhu. Untuk membuat lingkungan yang sesuai untuk axolotl tumbuh dan berkembang diperlukan bantuan alat. Salah satu alat yang digunakan adalah ultra recirculate chiller system (URCS). Penelitian ini bertujuan untuk mengetahui pengaruh manipulasi suhu yang berbeda terhadap pertumbuhan axolotl. Penelitian ini menggunakan metode eksperimen dengan penggunaan tiga perlakuan suhu yang berbeda (18°C, 22°C dan 26°C). Parameter utama yang diuji adalah tingkat kelulushidupan (SR), laju pertumbuhan spesifik (SGR) dan rasio konversi pakan (FCR). Hasil penelitian menunjukkan suhu berpengaruh terhadap tingkat kelulushidupan, laju pertumbuhan spesifik dan rasio konversi pakan. Perlakuan terbaik didapat pada perlakuan suhu 18°C dengan SR 100%, SGR 0,49%.