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All Journal Jurnal Akuakultur Indonesia The Journal of Experimental Life Sciences (JELS) Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Journal of Environmental Engineering and Sustainable Technology Jurnal Pembangunan dan Alam Lestari Journal of Aquatropica Asia JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal of Aquaculture and Fish Health Syntax Idea Journal Of Aquaculture Development and Environment Jurnal Perikanan Pantura Jurnal Penelitian Chanos Chanos Jurnal Pendidikan dan Pengabdian Masyarakat Journal of Fisheries & Marine Jurnal Perikanan
Abdul Rahem Faqih
Universitas Brawijaya
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Other

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Changes in Blood Glucose and Cortisol in Nile Tilapia (Oreochromis niloticus) with Different Salinities Satiti, Cinca Hayyu Putri; Faqih, Abdul Rahem; Mahmudi, Mohammad; Moehammad, Khibar Syiar
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.03

Abstract

Nile tilapia (Oreochromis niloticus) is a freshwater fish of high economic value that can tolerate a wide range of salinities, offering potential for the utilization of idle coastal ponds in northern Java. This study aimed to evaluate the physiological stress responses of Nile tilapia exposed to different salinity levels by measuring blood glucose levels, cortisol concentrations, and survival rates. The experiment was conducted for 28 days at the Sumberpasir Freshwater Fisheries Laboratory, Malang, using a Completely Randomized Design with four salinity treatments (0, 7, 14, and 21 ppt) in triplicate. Data on blood glucose and cortisol were analyzed using ANOVA (p < 0.05), while survival rate and water quality were analyzed descriptively. The results showed that salinity significantly affected blood glucose levels, with the highest value recorded at 21 ppt (115±39.2 mg.dL-1) and the lowest at 0 ppt (63±29.9 mg.dL-1). In contrast, cortisol levels were not significantly different among treatments, with the lowest average at 14 ppt (33.23±4.17 ng.mL-1). The highest survival rate was found at 0 and 7 ppt (100%), declining to 98% and 93% at 14 and 21 ppt, respectively. It is concluded that Nile tilapia can adapt physiologically to salinity levels of up to 21 ppt, with 14 ppt being the most stable level for stress adaptation. Further study is recommended to assess long-term growth and molecular indicators supporting the development of brackish water aquaculture.
Penurunan Motilitas dan Daya Fertilitas Sperma Ikan Lele Dumbo (Clarias spp) Pasca Perlakuan Stress Kejutan Listrik Faqih, Abdul Rahem
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1242.28 KB) | DOI: 10.21776/ub.jels.2011.001.02.03

Abstract

Transgenik pada ikan lele dumbo (Clarias spp) dengan menggunakan metode elektroporasi pada sperma ikan sebagai media transfer gen masih belum pernah dilakukan di Indonesia, sehingga perlu dilakukan percobaan pemberian tegangan pada sperma ikan lele dumbo tersebut. Tujuan dilakukan penelitian ini adalah untuk mengetahui pengaruh stress kejutan listrik (V) terhadap (motilitas) sperma dan daya fertilisasi sperma ikan lele dumbo. Penelitian ini dilaksanakan di Laboratorium Breeding (Budidaya Perairan) Fakultas Perikanan dan Ilmu Kelautan serta di Laboratorium Sentral Ilmu Hayati (LSIH) Universitas Brawijaya Malang, Jawa Timur mulai bulan Januari sampai Juni 2009. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 5 perlakuan 3 kali ulangan dan 1 kontrol. Sebagai perlakuan adalah perbedaan pemberian stress kejutan listrik dengan Gene pulser pada tegangan: A (40 V), B (160 V), C (280 V), D (400 V), E (520 V. Hasil penelitian menunjukkan bahwa pemberian stress kejutan listrik dengan tingkat voltase yang berbeda berpengaruh nyata terhadap motilitas sperma namun tidak berpengaruh nyata terhadap daya fertilisasi dan viabilitas sperma serta daya tetas telur. Berdasarkan analisis polynomial orthogonal didapatkan hubungan antara perlakuan tegangan dengan hasil motilitas sperma berbentuk linier dengan persamaan y=39,198-0,04x dengan R2=0,933 dan r=0,966. Semakin tinggi tegangan yang diberikan maka semakin rendah motilitas sperma yang dihasilkan. Dari hasil penelitian ini dapat disarankan bahwa elektroporasi pada sperma ikan lele dumbo (Clarias spp) sebagai media transfer gen sebaiknya dilakukan pada tegangan 40 Vcm-1 dengan satu kali kejutan dan lama kejutan 0,05 ms.Kata kunci: Clarias spp, sperma, elektroporasi, tegangan, transgenik
The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis Anitasari, Septi; Soeprijanto, Agus; Faqih, Abd. Rahem
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.131 KB) | DOI: 10.21776/ub.jels.2015.005.02.05

Abstract

Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer. Research is aimed to understand the effectiveness rate of hrGFP gene reporter to detect the successful gene transfer process based on the result of observation with convocal microscope over transgenic sperm level, embryo and larva of Carp Fish. Result of trans-genesis of hrGFP as sperm-mediated gene reporter must be accurately detected. Based on the result of observation with convocal microscope, it is shown that hrGFP enters the core level, indicates by PI pigmentation and thereby, hrGFP gene reporter can provide information about hrGFP fluorescence starting from sperm, embryo and larva of Carp Fish.Keywords: Carp Fish, effectiveness, gene reporter, hrGFP, sperm fluorescence.
Dynamics of Water Quality for Vannamei Shrimp Cultivation in Intensive Ponds in Coastal Areas Rizky Kusma Pratiwi; Mohammad Mahmudi; Abdul Rahem Faqih; Diana Arfiati
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4322

Abstract

Cultivators increase production using intensive cultivation which applies stocking densities and feeding in high quantities, which triggers a decrease in water quality, both cultivation water and cultivation wastewater because it contains high levels of organic matter. Changes in water quality have an impact on plankton community structure. This research aims to determine the dynamics of the physical, chemical, and biological quality of cultivation media water and the disposal of vaname shrimp cultivation waste in Ujungpangkah District, Gresik Regency. The research was conducted in January 2023 using a survey method. Water and plankton samples were taken from reservoirs, ponds, and pond waste disposal sites. Sampling was carried out in vaname shrimp cultivation ponds in the Coastal Area of ​​Ujungpangkah District, Gresik Regency. The physical and chemical quality of water has a pattern of changes up and down but is relatively the same and stable every week. All of these water quality parameters show a pattern of change that is quite good for the growth of vaname shrimp, but in waste disposal, the parameters for nitrate, phosphate, ammonia, and TOM are above the quality standards. The highest abundance of plankton is in waste dumps, with the highest phytoplankton coming from the Bacillariophyta phylum (49%), while zooplankton comes from the Rotifera phylum (54%). Regular monitoring of water quality in ponds and waste disposal sites is required so that it remains stable and safe for the environment.
Analisis Status Kualitas Air di Sungai Porong yang Tercemar oleh Deterjen terhadap Respon Hematologi Ikan Java Barb (Barbonymus gonionotus) Nico Rahman Caesar; Uun Yanuhar; Abd. Rahem Faqih; Septi Anitasari; Gatot Ciptadi; Muhammad Musa; Mohammad Bisri; Muhammad Sumsanto; Nezya Pramudya Wardani
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6170

Abstract

The Porong River is one of the rivers that faces high anthropogenic impacts, such as surfactant waste from detergents. The hematology of Javanese carp (Barbonymus gonionotus) serves as a sentinel organism that can be used as a biomarker for the waters of the Porong River. The aim of this study is to analyze the water quality status of the Porong River polluted with surfactants using the STORET method, as well as its impact on the hematology of Javanese carp using regression and correlation analysis. The research employed a descriptive survey method. The results of the study indicated that the Porong River was categorized as moderately polluted. The average highest surfactant concentration reached 7.2 mg/L, leading to an increase in TSS to 107.95 mg/L and a decrease in DO to 1.80 mg/L. Surfactants exhibited a negative correlation with erythrocytes (91.2%), hematocrit (47.7%), and hemoglobin (60.6%), indicating that the higher the surfactant concentration, the lower the number of erythrocytes, hematocrit levels, and hemoglobin levels in Javanese carp. Conversely, there was a positive correlation between surfactants and leukocytes (56.7%) and blood glucose (66.7%), suggesting that the higher the surfactant concentration, the higher the number of leukocytes and blood glucose levels in Javanese carp.
Revisi Peta Distribusi Ikan Padi (Adrianichthyidae: Oryzias spp.) Berdasarkan Identifikasi Morfologi di Paparan Sunda Indonesia Akhsan Fikrillah Paricahya; Kiki Nur Azam Kholil; Ifa Sufaichusan; Muh. Herjayanto; Rengga Retno Laila Saputri; Abd. Rahem Faqih; Agung Pramana Warih Marhendra
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6758

Abstract

Members of the Adrianichthyidae family share typical distributions, as does the javanicus group, which inhabits western parts of Southeast Asia. Javanicus group members, specifically Oryzias javanicus and Oryzias hubbsi, is the only Adrianichthyidae species that can be spotted in Sundaland Indonesia. Morphological identification and the species distribution mapping of Oryzias specimens from Sundaland in this study were interpreted using ecoregional and dendogram tree approach. Specimens were collected from 11 areas representing five ecoregions in Sundaland Indonesia. Specimens were collected from islands of Java, Sumatra, Belitung and Kalimantan. Six individuals from each population of Oryzias were sampled for morphological identification. Specimens collected from estuaries were morphologically identified as O. javanicus and specimens from freshwater identified as O. hubbsi. Distribution of O. hubbsi from Salatiga is the first record from Central East Java (CEJ) ecoregion, which was previously only distributed in Southern Sumatra-Western Java (SWJ) ecoregion. Morphology of O. hubbsi from Sundaland Indonesia has mean of Standard Length (SL) 1.56 cm, Total Length (TL) 1.94 cm, Anal rays (A) 16, Pectoral rays (P) 8, and Dorsal rays (D) 6. Meanwhile, morphology of O. javanicus from Sundaland has mean of SL 2.07 cm, TL 2.56 cm, A 22, P 11, and D 6.
Molecular Analysis Using Cytochrome Oxidase Subunit I (COI) to Confirming New Recorded Oryzias hubbsi in Central East Java Ecoregion Akhsan Fikrillah Paricahya; Kiki Nur Azam Kholil; Rengga Retno Laila Saputri; Muh. Herjayanto; Dewa Gede Raka Wiadnya; Abd. Rahem Faqih; Agung Pramana Warih Marhendra
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.6802

Abstract

Oryzias hubbsi's distribution is rarely discussed, as it is believed to only occupy the western part of Java, while its conservation status is Near Threatened (NT) as of 15 October 2018 on the IUCN red list. Specimens of O. hubbsi were recorded from Bandung and Jakarta in the description, other reports for O. hubbsi are commonly from Southern Sumatra-Western Java (SWJ) ecoregion in Java. However, an unexpected discovery of the genus Oryzias in Semarang freshwater opens up opportunities for the hidden spread of O. hubbsi to Central Java, and especially to other ecoregions which is Central and East Java (CEJ). Morphological approaches with distinctive O. hubbsi characters, complemented by molecular approaches with O. hubbsi samples from the Bogor population, confirmed similarities of species between both populations. Genetic distance using p-distances between both populations was below 2%, both populations made obvious clusters with a gap of 0.5%, and differed by five nucleotide base sites only. This new distribution record of O. hubbsi can be a foundation for conservation and domestication planning efforts.
Penerapan Teknologi Bioflok Pada Budidaya Ikan Nila Strain Braja 001 bagi Warga Binaan Lembaga Pemasyarakatan Perempuan (LPP) Kelas IIA Malang Abdul Rahem Faqih; Iwan Permadi; Agung Sugeng Widodo; Turniningtyas Ayu Rachmawati; Akhmad Muwafik Saleh; Septi Anitasari
Jurnal Pendidikan dan Pengabdian Masyarakat Vol. 9 No. 2 (2026): Mei
Publisher : FKIP Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppm.v9i2.11828

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan keterampilan dan kemandirian ekonomi warga binaan di Lembaga Pemasyarakatan Perempuan (LPP) Kelas IIA Malang melalui penerapan teknologi bioflok pada budidaya ikan nila strain Brawijaya 001 (Braja). Program ini merupakan bagian dari kolaborasi antara perguruan tinggi melalui skema Doktor Mengabdi dan Kuliah Kerja Nyata (KKN). Metode pelaksanaan meliputi penyuluhan, pelatihan, demonstrasi, serta pendampingan langsung dalam pengelolaan sistem bioflok, mulai dari persiapan media, penebaran benih, manajemen kualitas air, hingga pemanenan. Sebanyak ratusan benih ikan nila strain unggul ditebar dalam kolam budidaya berbasis bioflok yang efisien terhadap lahan dan penggunaan air. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan dan keterampilan warga binaan dalam budidaya ikan, serta terbentuknya aktivitas ekonomi  yang berpotensi menjadi bekal pasca pembinaan. Selain itu, program ini memperkuat sinergi antara akademisi dan lembaga pemasyarakatan dalam mendukung pemberdayaan perempuan dan pencapaian tujuan pembangunan berkelanjutan (SDGs). Dengan demikian, penerapan teknologi bioflok pada ikan nila Braja 001 menjadi model inovatif dalam pengembangan kewirausahaan berbasis perikanan di lingkungan terbatas.
Co-Authors Afnan, Naufal Izzudin Agung Pramana Warih Marhendra Agung Sugeng Widodo Agus Fery Setiawan Agus Soeprijanto Akhmad Muwafik Saleh Akhsan Fikrillah Paricahya Amalia, Elok Andi Kurniawan Ating Yuniarti Ayu Winna Ramadhani Ayuningtyas Ayuningtyas Czech, Michael Danang Yonarta Dewa Gede Raka Wiadnya Dian Kartika Sari Diana Aisyah Diana Arfiati Eka Fitria Febriani Eka Fitria Febriani F Fuad Frits Jamlaay, Frits G Guntur Gatot Ciptadi Harahap, Muhammad Alfiandi Rachmad Ifa Sufaichusan Islamy, R. Adharyan Isroni, Wahyu Iwan Permadi Kiki Nur Azam Kholil Kiki Nur Azam Kholil Kurniawan , Andi Kusuma, Baruna Kusuma, Wahyu Endra M. Amenan Maheno Sri Widodo Maheno Sri Widodo, Maheno Sri Moehammad, Khibar Syiar MOHAMAD FADJAR Mohammad Bisri Mohammad Mahmudi Muh. Herjayanto Muhammad Dailami, Muhammad Muhammad Musa Muhammad Sumsanto Nezya Pramudya Wardani Nico Rahman Caesar Novalina Serdiati Putra, Arya Darmawan Kurniadi R Adharyan Islamy Ramadhani, Auryn Novia Rengga Retno Laila Saputri Rizal Akbar Hutagalung, Rizal Akbar Rizky Kusma Pratiwi Rohmah Widiastuti Sanoesi, Ellana Sansi, Delfina Husniya Satiti, Cinca Hayyu Putri Septi Anitasari Septi Anitasari Septi Anitasari, Septi Sufaichusan, Ifa Susilowati, Surya Rachman tholibah mujtahidah Turniningtyas Ayu Rachmawati Veryl Hasan Wahyu Endra Kusuma Yanuhar, Uun Yuni Kilawati Yunita Maimunah Zulkisam Pramudia