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Dynamics of Water Quality for Vannamei Shrimp Cultivation in Intensive Ponds in Coastal Areas Pratiwi, Rizky Kusma; Mahmudi, Mohammad; Faqih, Abdul Rahem; Arfiati, Diana
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4322

Abstract

Cultivators increase production using intensive cultivation which applies stocking densities and feeding in high quantities, which triggers a decrease in water quality, both cultivation water and cultivation wastewater because it contains high levels of organic matter. Changes in water quality have an impact on plankton community structure. This research aims to determine the dynamics of the physical, chemical, and biological quality of cultivation media water and the disposal of vaname shrimp cultivation waste in Ujungpangkah District, Gresik Regency. The research was conducted in January 2023 using a survey method. Water and plankton samples were taken from reservoirs, ponds, and pond waste disposal sites. Sampling was carried out in vaname shrimp cultivation ponds in the Coastal Area of ​​Ujungpangkah District, Gresik Regency. The physical and chemical quality of water has a pattern of changes up and down but is relatively the same and stable every week. All of these water quality parameters show a pattern of change that is quite good for the growth of vaname shrimp, but in waste disposal, the parameters for nitrate, phosphate, ammonia, and TOM are above the quality standards. The highest abundance of plankton is in waste dumps, with the highest phytoplankton coming from the Bacillariophyta phylum (49%), while zooplankton comes from the Rotifera phylum (54%). Regular monitoring of water quality in ponds and waste disposal sites is required so that it remains stable and safe for the environment.
Analisis Status Kualitas Air di Sungai Porong yang Tercemar oleh Deterjen terhadap Respon Hematologi Ikan Java Barb (Barbonymus gonionotus) Caesar, Nico Rahman; Yanuhar, Uun; Faqih, Abd. Rahem; Anitasari, Septi; Ciptadi, Gatot; Musa, Muhammad; Bisri, Mohammad; Sumsanto, Muhammad; Wardani, Nezya Pramudya
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6170

Abstract

The Porong River is one of the rivers that faces high anthropogenic impacts, such as surfactant waste from detergents. The hematology of Javanese carp (Barbonymus gonionotus) serves as a sentinel organism that can be used as a biomarker for the waters of the Porong River. The aim of this study is to analyze the water quality status of the Porong River polluted with surfactants using the STORET method, as well as its impact on the hematology of Javanese carp using regression and correlation analysis. The research employed a descriptive survey method. The results of the study indicated that the Porong River was categorized as moderately polluted. The average highest surfactant concentration reached 7.2 mg/L, leading to an increase in TSS to 107.95 mg/L and a decrease in DO to 1.80 mg/L. Surfactants exhibited a negative correlation with erythrocytes (91.2%), hematocrit (47.7%), and hemoglobin (60.6%), indicating that the higher the surfactant concentration, the lower the number of erythrocytes, hematocrit levels, and hemoglobin levels in Javanese carp. Conversely, there was a positive correlation between surfactants and leukocytes (56.7%) and blood glucose (66.7%), suggesting that the higher the surfactant concentration, the higher the number of leukocytes and blood glucose levels in Javanese carp.
Revisi Peta Distribusi Ikan Padi (Adrianichthyidae: Oryzias spp.) Berdasarkan Identifikasi Morfologi di Paparan Sunda Indonesia Paricahya, Akhsan Fikrillah; Kholil, Kiki Nur Azam; Sufaichusan, Ifa; Herjayanto, Muh.; Saputri, Rengga Retno Laila; Faqih, Abd. Rahem; Marhendra, Agung Pramana Warih
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6758

Abstract

Members of the Adrianichthyidae family share typical distributions, as does the javanicus group, which inhabits western parts of Southeast Asia. Javanicus group members, specifically Oryzias javanicus and Oryzias hubbsi, is the only Adrianichthyidae species that can be spotted in Sundaland Indonesia. Morphological identification and the species distribution mapping of Oryzias specimens from Sundaland in this study were interpreted using ecoregional and dendogram tree approach. Specimens were collected from 11 areas representing five ecoregions in Sundaland Indonesia. Specimens were collected from islands of Java, Sumatra, Belitung and Kalimantan. Six individuals from each population of Oryzias were sampled for morphological identification. Specimens collected from estuaries were morphologically identified as O. javanicus and specimens from freshwater identified as O. hubbsi. Distribution of O. hubbsi from Salatiga is the first record from Central East Java (CEJ) ecoregion, which was previously only distributed in Southern Sumatra-Western Java (SWJ) ecoregion. Morphology of O. hubbsi from Sundaland Indonesia has mean of Standard Length (SL) 1.56 cm, Total Length (TL) 1.94 cm, Anal rays (A) 16, Pectoral rays (P) 8, and Dorsal rays (D) 6. Meanwhile, morphology of O. javanicus from Sundaland has mean of SL 2.07 cm, TL 2.56 cm, A 22, P 11, and D 6.
Molecular Analysis Using Cytochrome Oxidase Subunit I (COI) to Confirming New Recorded Oryzias hubbsi in Central East Java Ecoregion Paricahya, Akhsan Fikrillah; Kholil, Kiki Nur Azam; Saputri, Rengga Retno Laila; Herjayanto, Muh.; Wiadnya, Dewa Gede Raka; Faqih, Abd. Rahem; Marhendra, Agung Pramana Warih
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.6802

Abstract

Oryzias hubbsi's distribution is rarely discussed, as it is believed to only occupy the western part of Java, while its conservation status is Near Threatened (NT) as of 15 October 2018 on the IUCN red list. Specimens of O. hubbsi were recorded from Bandung and Jakarta in the description, other reports for O. hubbsi are commonly from Southern Sumatra-Western Java (SWJ) ecoregion in Java. However, an unexpected discovery of the genus Oryzias in Semarang freshwater opens up opportunities for the hidden spread of O. hubbsi to Central Java, and especially to other ecoregions which is Central and East Java (CEJ). Morphological approaches with distinctive O. hubbsi characters, complemented by molecular approaches with O. hubbsi samples from the Bogor population, confirmed similarities of species between both populations. Genetic distance using p-distances between both populations was below 2%, both populations made obvious clusters with a gap of 0.5%, and differed by five nucleotide base sites only. This new distribution record of O. hubbsi can be a foundation for conservation and domestication planning efforts.
Changes in Blood Glucose and Cortisol in Nile Tilapia (Oreochromis niloticus) with Different Salinities Satiti, Cinca Hayyu Putri; Faqih, Abdul Rahem; Mahmudi, Mohammad; Moehammad, Khibar Syiar
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.03

Abstract

Nile tilapia (Oreochromis niloticus) is a freshwater fish of high economic value that can tolerate a wide range of salinities, offering potential for the utilization of idle coastal ponds in northern Java. This study aimed to evaluate the physiological stress responses of Nile tilapia exposed to different salinity levels by measuring blood glucose levels, cortisol concentrations, and survival rates. The experiment was conducted for 28 days at the Sumberpasir Freshwater Fisheries Laboratory, Malang, using a Completely Randomized Design with four salinity treatments (0, 7, 14, and 21 ppt) in triplicate. Data on blood glucose and cortisol were analyzed using ANOVA (p < 0.05), while survival rate and water quality were analyzed descriptively. The results showed that salinity significantly affected blood glucose levels, with the highest value recorded at 21 ppt (115±39.2 mg.dL-1) and the lowest at 0 ppt (63±29.9 mg.dL-1). In contrast, cortisol levels were not significantly different among treatments, with the lowest average at 14 ppt (33.23±4.17 ng.mL-1). The highest survival rate was found at 0 and 7 ppt (100%), declining to 98% and 93% at 14 and 21 ppt, respectively. It is concluded that Nile tilapia can adapt physiologically to salinity levels of up to 21 ppt, with 14 ppt being the most stable level for stress adaptation. Further study is recommended to assess long-term growth and molecular indicators supporting the development of brackish water aquaculture.
THE RANGE EXPANSION OF Parachromis managuensis (GÜNTHER, 1867) (PERCIFORMES, 4 CICHLIDAE) IN JAVA, INDONESIA Hasan, Veryl; Faqih, Abdul Rahem; Maftuch, Maftuch
BIOTROPIA Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.606 KB) | DOI: 10.11598/btb.2022.29.1.1278

Abstract

The Jaguar cichlid, Parachromis managuensis (Günther, 1867), is native to Central America, with introduc­tions reported from West Java and Central Java Provinces of Indonesia. On 7-8 January 2019, sixteen specimens of P. managuensis were collected from Karangkates, the largest hydropower reservoir in East Java Province of Indonesia. A description of the morphological characters of specimens is provided.
Growth Performance and Osmoregulation of Vaname Shrimp (Litopenaeus Vannamei) with Cultivation Media Engineering Aldy Eka Wahyudi; Abdul Rahem Faqih; Sri Andayani
International Journal of Marine Engineering Innovation and Research Vol. 10 No. 4 (2025)
Publisher : Department of Marine Engineering, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25481479.v10i4

Abstract

Production of white shrimp (Litopenaeus vannamei) in 2024 is targeted by the Ministry of Fisheries and Marine Affairs to be increased by producing 2 million tons / year. However, in fact, in 2022 white shrimp production is 1.09 million tons, so to achieve the target, new technology and innovation are needed. The obstacle experienced with freshwater cultivation is the low mineral that plays an important role in the growth of white shrimp. To be able to cultivate white shrimp in fresh water, it is necessary to first conduct a study on the manipulation of aquaculture water with freshwater, salt and bittern components as mineral sources. The purpose of this study is to test the combination of components of artificial water media for white shrimp farming and analyze the mineral content. The study will be conducted from March to April 2024. The method used is an experimental method with Completely Randomized Design (CRD). Data collection is carried out by field observation and literature study. The research procedures carried out were bittern and salt collection and white shrimp sampling, media preparation, mineral test on water as culture media, white shrimp stocking, white shrimp rearing, morphology and survival rate tests and viscosity tests to review osmoregulation in white shrimp and data analysis. Data analysis was performed using Canonical Correlation Analysis (CCA) with PAST 4.03 software. The mineral content produced is directly proportional to the amount of dose given. The best dose of bittern in this study is in treatment C with a dose of 15 ml which has a calcium content of 45.2 mg/L, magnesium 155 mg/L, and potassium 73.89 mg/L.
Penurunan Motilitas dan Daya Fertilitas Sperma Ikan Lele Dumbo (Clarias spp) Pasca Perlakuan Stress Kejutan Listrik Faqih, Abdul Rahem
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1242.28 KB) | DOI: 10.21776/ub.jels.2011.001.02.03

Abstract

Transgenik pada ikan lele dumbo (Clarias spp) dengan menggunakan metode elektroporasi pada sperma ikan sebagai media transfer gen masih belum pernah dilakukan di Indonesia, sehingga perlu dilakukan percobaan pemberian tegangan pada sperma ikan lele dumbo tersebut. Tujuan dilakukan penelitian ini adalah untuk mengetahui pengaruh stress kejutan listrik (V) terhadap (motilitas) sperma dan daya fertilisasi sperma ikan lele dumbo. Penelitian ini dilaksanakan di Laboratorium Breeding (Budidaya Perairan) Fakultas Perikanan dan Ilmu Kelautan serta di Laboratorium Sentral Ilmu Hayati (LSIH) Universitas Brawijaya Malang, Jawa Timur mulai bulan Januari sampai Juni 2009. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 5 perlakuan 3 kali ulangan dan 1 kontrol. Sebagai perlakuan adalah perbedaan pemberian stress kejutan listrik dengan Gene pulser pada tegangan: A (40 V), B (160 V), C (280 V), D (400 V), E (520 V. Hasil penelitian menunjukkan bahwa pemberian stress kejutan listrik dengan tingkat voltase yang berbeda berpengaruh nyata terhadap motilitas sperma namun tidak berpengaruh nyata terhadap daya fertilisasi dan viabilitas sperma serta daya tetas telur. Berdasarkan analisis polynomial orthogonal didapatkan hubungan antara perlakuan tegangan dengan hasil motilitas sperma berbentuk linier dengan persamaan y=39,198-0,04x dengan R2=0,933 dan r=0,966. Semakin tinggi tegangan yang diberikan maka semakin rendah motilitas sperma yang dihasilkan. Dari hasil penelitian ini dapat disarankan bahwa elektroporasi pada sperma ikan lele dumbo (Clarias spp) sebagai media transfer gen sebaiknya dilakukan pada tegangan 40 Vcm-1 dengan satu kali kejutan dan lama kejutan 0,05 ms.Kata kunci: Clarias spp, sperma, elektroporasi, tegangan, transgenik
The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis Anitasari, Septi; Soeprijanto, Agus; Faqih, Abd. Rahem
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.131 KB) | DOI: 10.21776/ub.jels.2015.005.02.05

Abstract

Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer. Research is aimed to understand the effectiveness rate of hrGFP gene reporter to detect the successful gene transfer process based on the result of observation with convocal microscope over transgenic sperm level, embryo and larva of Carp Fish. Result of trans-genesis of hrGFP as sperm-mediated gene reporter must be accurately detected. Based on the result of observation with convocal microscope, it is shown that hrGFP enters the core level, indicates by PI pigmentation and thereby, hrGFP gene reporter can provide information about hrGFP fluorescence starting from sperm, embryo and larva of Carp Fish.Keywords: Carp Fish, effectiveness, gene reporter, hrGFP, sperm fluorescence.