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Optimization and Validation of Electrochemical Biosensor Based on Pseudomonas sp. Biofilm Immobilized on Screen-Printed Carbon Electrode in Detecting Benzene Alfiah Alif; Dyah Iswantini; Henny Purwaningsih; Novik Nurhidayat; Ali Aulia Ghozali; Amalyah Febryanti
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 15, No 1: June 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v15i1.19008

Abstract

Benzene is known as one of the hazardous compounds potentially interfering the health and polluting the environment. Generally, detecting benzene still requires a long analysis time and expensive costs. Therefore, this study aimed to evaluate the analytical performance of a biosensor with microbes as the biodetection element. Pseudomonas sp. biofilms which produce benzene dioxygenase enzyme and then immobilized on a Screen-Printed Carbon Electrode (SPCE). The results of the optimization of the biosensor obtained a benzene concentration of 3 mM, a bacterial density of 1.4x1011 cells/mL and suspension pH of 7.5. The optimization results are used to measure the analytical performance of the biosensor. The value of analytical performance produced on linearity is in the measurement range of 0.1 - 3 mM, the equation y = 7.4118x + 80.048 with R2 = 0.9744. The detection limit and quantity limit are 0.5630 mM and 1.8767 mM respectively, with a sensitivity of 7.4188 μA/mM. The precision obtained shows that the SPCE biofilm method has moderate accuracy with a %SBR value below 5%. The selectivity of this method still needs to increase, but the stability of the benzene biosensor increases up to 35 days with an activity of 100.36%. This indicates the immobilization of Pseudomonas sp. had potency as an alternative method for detecting benzene and it can be developed for a prototype.
Tyrosinase-Based Paper Biosensor for Phenolics Measurement Fretty Yurike; Dyah Iswantini; Henny Purwaningsih; Suminar Setiati Achmadi
Indonesian Journal of Chemistry Vol 22, No 5 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.72607

Abstract

Environmental pollution resulting from various industrial activities is still a problem for developing countries. The high content of phenolics such as phenols, polyphenols, bisphenol A, catechol, m- and p-cresol from industrial activities are discharged into surface water, soil, and air. Periodic monitoring of the impact of these toxic pollutants is needed for proper control and handling. These detrimental chemicals are usually measured using conventional methods with many drawbacks such as expensive analysis costs, long measurement times, requiring competent analysts, and complicated instrument maintenance. However, the presence of tyrosinase-based paper biosensors is now considered the most promising tool in overcoming the challenges mentioned earlier because they can detect these components quickly, precisely, accurately, inexpensively, and can be measured in situ. The working principle of this biosensor sees optical changes such as dyes, redox processes, and physicochemical properties (aggregation or dispersion) due to the presence of analytes accompanied by the occurrence of color changes that appear. This biosensor uses a layer-by-layer electrostatic method, which causes the deposition of multi-layered films on solid surfaces. In this paper, we review the development of the tyrosinase-based paper biosensor method for phenolic measurement in water, air, and food that gives better results than the conventional methods.
Predictor Analysis of Antenatal Care Behavior in the Pandemic Era in the Work Area of the Banyuwangi Kabat Health Center Rahmawati Raharjo; Henny Purwaningsih; Gufron Wahyudi
Journal for Quality in Public Health Vol. 5 No. 2 (2022): May
Publisher : Master of Public Health Program Institut Ilmu Kesehatan STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/jqph.v5i2.363

Abstract

The spread of COVID-19 caused by the SARS-CoV-2 virus has spread worldwide . On January 30, 2020, WHO declared it a public health emergency (Suryani & Purwodiharjo, 2021). Indonesia is currently expanding across regions, followed by an increase in the number of cases and the number of deaths (Purba, 2021). Overview of the Death Rate from Positive COVID-19 Cases Per 100,000 Population Based on Regency/City, Banyuwangi is ranked 8th at the national level at 396 with the highest number of deaths.ANC is a visit by pregnant women with health workers aimed at monitoring the progress of pregnancy to ensure the health of the mother and baby's growth and development, to recognize any abnormalities or complications that may occur during pregnancy, prepare for childbirth, prepare for the postpartum period and provide exclusive breastfeeding. (Fatkhiyah , d kk, 2020). This type of research is quantitative using observational analytical methods using a cross sectional approach. The variables in this study consisted of independent variables and dependent variables. The independent variable isbehavioral factors (age, occupation, knowledge, and attitude). The dependent variable is ANC (antenatal care) behavior . The number of samples in this study were 30, using total sampling technique. Data collection techniques were carried out by distributing questionnaires. The analysis in this study used Multiple Linear Regression Test consisting of t test and F test.
APLIKASI REAKSI KATALISIS HETEROGEN UNTUK PEMBUATAN VANILI SINTETIK (3-HIDROKSI-2-METOKSIBENZALDEHIDA) DARI EUGENOL (4-ALLIL-2-METOKSIFENOL) MINYAK CENGKEH Wibowo, Widajanti; Suwarso, Wahyudi Priyono; Utari, Triesye; Purwaningsih, Henny
Makara Journal of Science Vol. 6, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

APLIKASI REAKSI KATALISIS HETEROGEN UNTUK PEMBUATAN VANILI SINTETIK (3-HIDROKSI-2-METOKSIBENZALDEHIDA) DARI EUGENOL (4-ALLIL-2-METOKSIFENOL) MINYAK CENGKEH Wibowo, Widajanti; Suwarso, Wahyudi Priyono; Utari, Triesye; Purwaningsih, Henny
Makara Journal of Science Vol. 6, No. 3
Publisher : UI Scholars Hub

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Abstract

Synthesis and Characterization of Corn Husk (Zea Mays L.) Cellulose Using Microwave-Assisted Extraction (MAE) Nor Pana Yupa; Henny Purwaningsih; Tetty Kemala
Jurnal Kimia Sains dan Aplikasi Vol 26, No 7 (2023): Volume 26 Issue 7 Year 2023
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.26.7.268-275

Abstract

This research focuses on synthesizing cellulose from corn husks using microwave-assisted extraction (MAE), which aims to produce cellulose from corn husks through liquefaction, delignification, and bleaching processes. The total time required is only 14 minutes, thus shortening the process time. This study used three power variations: 450 W, 600 W, and 800 W. The results showed that the optimum conditions occurred at 800 W power. Hence, the higher the power caused the high cellulose content obtained, which amounted to 75.23% and decreased the lignin value to 15.78%, along with the increase in microwave power. FTIR analysis also confirmed the cellulose results obtained and indicated that the lignin groups were weakened or lost. Furthermore, morphological analysis of cellulose fibers by SEM showed that the surface of cellulose fibers was rough, oval, and irregular due to cracks caused by erosion. Then, the result of cellulose crystallinity using XRD was 45.8%. Compared to conventional methods, microwave-assisted cellulose synthesis exhibits better and more promising potential. This is because microwave radiation can be used to improve process time efficiency and achieve higher yields. Moreover, the proposed method is easier, faster, and straightforward.
Optimization of Cadmium Removal Using Tetraethylene Glycol-Modified Silica-Based Adsorbent via Response Surface Methodology Roza Linda; Abdullah Abdullah; Afrianto Daud; Lisa Aprilia Indriyani; Henny Purwaningsih; Mohamad Rafi; Lee Wah Lim
Jurnal Kimia Sains dan Aplikasi Vol 27, No 3 (2024): Volume 27 Issue 3 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.3.128-136

Abstract

In solid-phase extraction for preconcentration, silica (Si) is the most commonly used as an adsorbent. However, the selectivity and effectiveness of silica gel adsorption on metal ions are low, so it needs to be modified to improve the adsorption capability. The modification was done using reflux and oven heating in the modification silica with 3-glycidoxypropyl trimethoxysilane (GPTMS) and tetraethylene glycol (TEG). A central composite design batch process determined the optimum conditions for cadmium adsorption. TEG-modified silica was successfully synthesized and characterized using FTIR spectroscopy, SEM, and elemental analyzers. Peaks of C-H and epoxy on FTIR spectra showed that Si- GPTMS was formed. The increase of %C and %H from the first to the second step indicated that Si-TEG was successfully synthesized. There was no significant difference in silica particle morphology on SEM before and after modification. The reflux method gave a higher yield compared to the heating method. The constant stirring by the magnetic bar and solvent cycle in the reflux method catalyzed the reaction. This study found that at pH 7, 30 mg of adsorbent weight at 35°C and 22 minutes of contact time were optimum Cd2+ adsorption conditions. As the weight of the adsorbent increases, the adsorption capacity decreases. Contact time and temperature have no significant effect on Cd adsorption by Si-TEG.
ANALISIS PERILAKU ISOTERM, TERMODINAMIKA, DAN KINETIKA ADSORPSI STRONTIUM MENGGUNAKAN TiO2 – ZEOLIT LAMPUNG Sholahudin, M. Fiqri; Purwaningsih, Henny; Noerpitasari, Erlina; Boybul, Boybul; Yanlinastuti, Yanlinastuti
Urania : Jurnal Ilmiah Daur Bahan Bakar Nuklir Vol 29, No 2 (2023): OKTOBER, 2023
Publisher : website

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/urania.2023.29.2.6840

Abstract

Bahan bakar nuklir setelah digunakan di reaktor  menghasilkan beberapa hasil fisi, dimana salah satunya adalah strontium (90Sr). Isotop strontium dapat dimanfaatkan menjadi bahan baku pembuatan baterai, tetapi strontium tersebut masih tercampur dengan hasil fisi atau isotop lainnya sehingga diperlukan proses pemisahan. Salah satu metode pemisahan strontium adalah dengan adsorpsi. Dalam penelitian ini, campuran TiO2-zeolit Lampung dievaluasi kinerjanya sebagai adsorben sehingga dapat digunakan untuk adsorpsi strontium. Zeolit Lampung awal diaktivasi menggunakan asam sulfat pekat untuk membersihkan pori-pori yang masih mengandung pengotor. Kemudian zeolit dicampurkan dengan TiO2 menggunakan metode stir casting. Adsorben TiO2-zeolit Lampung yang diperoleh kemudian dikarakterisasi menggunakan Fourier Transform Infrared (FTIR), X-Ray Diffraction Spectrometer (XRD), dan Scanning Electron Microscope (SEM). Setelah itu dilakukan uji adsorpsi dengan model isoterm, termodinamika, dan kinetika. Terbentuknya TiO2-zeolit yang dicirikan menggunakan spektroskopi FTIR  ditunjukkan dengan adanya puncak serapan Ti-O-Si pada zeolit Lampung setelah ditambahkan TiO2. Hasil analisis menggunakan XRD memperlihatkan zeolit Lampung berjenis klinoptilolit dan mordenit, serta TiO2 yang digunakan memiliki fase anatase. Hasil analisis menggunakan SEM mengindikasikan TiO2 tersebar pada permukaan zeolit Lampung. Model isoterm adsorpsi untuk adsorben TiO2-zeolit memenuhi model isoterm Langmuir. Kajian termodinamika menunjukkan proses adsorpsi bersifat endoterm dan tidak spontan, dan kinetika adsorpsi strontium mengikuti orde kedua semu.Kata kunci: Isoterm, adsorpsi, strontium, TiO2, zeolit.
Isolation and Characterization of Cellulose Nanofibrils (CNF) from Dates by-Product via Citric Acid Hydrolysis Aisy, Laaili Azti Rohaadatul; Kemala, Tetty; Suryanegara, Lisman; Purwaningsih, Henny
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.818-827

Abstract

Industrial residues that are not optimally utilized are removed by burning, landfilling, or dumping, which can threaten the environment and health. In fact, part of this agro-industrial waste still has content that has the potential to become raw material for value-added other industries. Dates by-product as residue of the fiber-rich fruit industry have the potential to be a source of nanocellulose. This study aims to obtain nanofibril cellulose (CNF) isolates from dates by-product via citric acid hydrolysis, and investigate the effect of acid concentration on the unknown dates by-product CNF isolate characteristics. Pretreatment such as delignification and bleaching is needed to obtain cellulose isolate with high purity. Furthermore, acid hydrolysis, centrifugation, and sonication are performed to obtain CNF. CNF isolates are characterized by the analysis of particle size distribution, morphology, and crystallinity. Analysis of functional groups and lignocellulose content test confirm that lignin and hemicellulose are degraded during isolation. The particle size distribution measurement shows that the greater the acid concentration, the smaller the CNF size and the better the size uniformity. The morphology of the CNF obtained is net-like fibers. The degree of crystallinity shown decreases with increasing acid concentration. This study revealed that different citric acid concentrations can result in different characteristics of CNF isolates.
Readiness Dosen dalam Mengintegrasikan Kecerdasan Buatan untuk Pengajaran Menulis Teks Akademik di Perguruan Tinggi Putera, Zulmy Faqihuddin; Shofiah, Nurul; Ramadhani, Rizki Putri; Maulidina, Ahsani; Puspitasari, Peni; Purwaningsih, Henny
Jurnal Pendidikan Bahasa dan Sastra Indonesia Metalingua Vol 9, No 2 (2024): Metalingua, Edisi Oktober 2024
Publisher : Program Studi Pendidikan Bahasa dan Sastra Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/metalingua.v9i2.27713

Abstract

. Penelitian ini bertujuan untuk menilai kesiapan dosen di perguruan tinggi polinema dalam mengintegrasikan Kecerdasan Buatan (AI) dalam pendidikan untuk penulisan tugas akademik dengan fokus pada persepsi, tantangan, dan kebutuhan pelatihan mereka. Metodologi kualitatif digunakan, dengan menggunakan Desain yang didasarkan pada prinsip-prinsip dasar Technology Acceptance Model (TAM) yang diusulkan oleh Davis (1989) Dale Viethen (2021) dan Nazari dkk. (2021). Subjek penelitian yakni 10 dosen di dua Universitas Malang. panduan yang dikembangkan dan disempurnakan melalui tinjauan sejawat ahli dan studi percontohan ini mencakup sikap terhadap AI, manfaat dan tantangan yang dirasakan dari AI dalam pengajaran kemahiran diri dalam menggunakan AI, dan niat perilaku terkait penggunaannya. Hasilnya menunjukkan tingkat kesiapan dosen yang berbeda-beda, yang dipengaruhi oleh latar belakang pemahaman teknologi, dan kekhawatiran akan privasi dan keamanan data. Meskipun ada rasa optimisme secara umum tentang potensi AI, tantangan signifikan terkait akses sumber daya yang terbatas dan pengembangan profesional yang tidak memadai telah diidentifikasi. Hasil penelitian ini menekankan perlunya inisiatif kebijakan yang ditargetkan dan program pelatihan untuk meningkatkan kesiapan dosen dalam mengadopsi AI. Wawasan dari penelitian ini berkontribusi dalam memahami fasilitator dan hambatan integrasi AI dalam pendidikan, menyoroti peran penting kesiapan dosen dalam pemanfaatan AI yang efektif dalam konteks pendidikan. KATA KUNCI: Kesiapan AI, Adopsi AI, kecerdasan buatan, penulisan teks akademik, perguruan tinggi