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All Journal HAYATI Journal of Biosciences VALENSI JURNAL KIMIA SAINS DAN APLIKASI Urania Jurnal Ilmiah Daur Bahan Bakar Nuklir Jurnal Riset Kimia Jurnal Kimia dan Kemasan EduChemia: Jurnal Kimia dan Pendidikan Indonesia Chimica Acta Science and Technology Indonesia Al-Kimia Indonesian Journal of Chemistry Jurnal Pendidikan Bahasa dan Sastra Indonesia Metalingua Jurnal Kimia dan Kemasan Jurnal Pijar MIPA Makara Journal of Science Journal for Quality in Public Health Journal for Research in Public Health
Henny Purwaningsih
Chemistry Department, Faculty Of Mathematics And Natural Science, IPB University, Kampus IPB Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Nursing Physics Public Health

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Decolorization of Synthetic Dyes by Laccase of Phellinus noxius BRB 11 Immobilized with Halloysite Nanotubes Naser, Inda Hastuti N; Kemala, Tetty; Yanto, Dede Heri Yuli; Purwaningsih, Henny; Nurhayat, Oktan Dwi; Ardiati, Fenny Clara; Bakar, N.H.H Abu; Iqbal, Anwar
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.644-659

Abstract

Halloysite nanotubes (HNTs) have been proposed as promising supports for enzyme immobilization. Developing an effective technique for enzyme immobilization on HNTs is crucial for their practical application. In this study, we present a method for immobilizing laccase enzymes on HNTs using various amino acids as green cross-linkers for eco-friendly decolorization of synthetic dye. The modified HNTs were characterized using FT-IR, FE-SEM, and HR-TEM analyses. Furthermore, the effects of various parameters—including pH (ranging from 3 to 8), temperature (30-70°C), toxicity, and reusability—on the activity of both free and immobilized laccase were investigated. The results indicated that glycine (Gly) provided the most effective support for laccase immobilization on HNTs. The modified HNTs/Gly-Lac achieved an immobilization yield of 98.45% and successfully decolorized 94.60% of Acid Blue 129 (AB129) within 2 h. The successful immobilization of laccase onto functionalized HNTs was confirmed through FT-IR, FE-SEM, and HR-TEM analyses. Morphological observations of HNTs/Gly for laccase immobilization showed a thickened and roughened appearance with a thin, light gray layer on the outer surface. Additionally, the presence of an imine (-C=N-) bond peak and a peak at 1410 cm-1, corresponding to the (-NH) bond, suggested successful covalent immobilization. The toxicity analysis revealed a reduction in the toxicity of laccase after immobilization onto modified HNTs. A reusability assessment conducted over five cycles demonstrated that decolorization efficiency remained above 45%. These findings suggest that HNTs/GlyLac could serve as an effective material for textile dye wastewater treatment.
Descriptive Analysis Performance Analysis, Satisfaction Level and Program Achievement Based on Leadership Profession Head of Public Health Center Throughout Banyuwangi District 2018 Purwaningsih, Henny; Siyoto, Sandu; Koesnadi
Journal for Research in Public Health Vol. 1 No. 2 (2020): January
Publisher : Master of Public Health Program Universitas STRADA Indonesia

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Abstract

Health management is an activity or an art to regulate health workers and non-public health officers through health programs. One of the objectives of managing human resources in organizational management functions is related to leadership problems. Someone who has been appointed as a leader to lead his subordinates, he must carry out the function of the organization itself which determines the success or failure of a company. The purpose of the study was to analyze the descriptive performance, level of satisfaction and achievement of the program based on the professional leadership of the head of the Public health throughout Banyuwangi District. The research design used was cross sectional. The population of the study was all heads of administration of Public health throughout Banyuwangi District and the sample size was 45 people using total sampling. Data collection using sheets and questionnaires was then analyzed using the Friedman test. The results showed that the profession of physician leadership most of the performance of high category as many as 17 respondents (70.8%), and professional leadership performance Bachelor of Public Health mostly high category, a total of 18 respondents (85.7%), physician leadership professions most levels of satisfaction satisfied category as many as 20 respondents (83.3%) , and the Bachelor of Public Health leadership profession were mostly satisfied category performance, namely as many as 17 respondents (81.0%) and the leadership profession the majority of the doctor's program achievements and Bachelor of Public Health good categories were as much as 100%. Based on this research, he can improve his skills and professionalism at work
Isolation and Characterization of Cellulose Whiskers from Lampung Sugarcane Bagasse, Indonesia Afifi, Muhammad Ridho; Aini, Zahratul; Irawadi, Tun Tedja; Purwaningsih, Henny
Jurnal Pijar Mipa Vol. 20 No. 5 (2025)
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i5.9371

Abstract

Sugarcane bagasse, the fibrous residue from sugar production, is an abundant agricultural waste in Indonesia, especially in Lampung, one of the country’s leading sugarcane-producing provinces. Its high lignocellulosic content makes it a promising alternative source of cellulose. However, effective extraction and conversion into high-value products such as cellulose whiskers require optimized chemical processes. This study aims to isolate cellulose from sugarcane bagasse using alkali and peroxide treatments and convert it into cellulose whiskers through hydrolysis using sulfuric acid (H₂SO₄) at varying concentrations (4–12 M). The objective is to evaluate the optimal acid concentration that produces cellulose whiskers with desirable morphology and crystallinity. Cellulose isolation was achieved through sequential NaOH and H₂O₂ treatments, significantly reducing lignin and hemicellulose content and yielding α-cellulose at 88.37%. Hydrolysis of the purified cellulose was then performed with H₂SO₄. The resulting materials were characterized using FTIR, SEM, XRD, and TGA. FTIR confirmed the removal of non-cellulosic components, while SEM showed that only 10 M H₂SO₄ produced well-defined whiskers with nanoscale dimensions (200–700 nm in length and 10–50 nm in diameter). Lower acid concentrations resulted in incomplete hydrolysis, while excessive degradation occurred at 12 M. XRD analysis revealed an increase in crystallinity to 86.7%, indicating the removal of amorphous regions and successful formation of crystalline whiskers. TGA analysis showed different thermal degradation patterns between isolated cellulose and whiskers, supporting structural transformation. Cellulose whiskers can subsequently be utilized as a reinforcing material in the fabrication of plastic films for water–oil separation and dye adsorption applications in laboratory experiments.
Co-Authors A. Sitompul A. Sitompul A.A. Ketut Agung Cahyawan W Abdullah Abdullah Afifi, Muhammad Ridho Afrianto Daud Aini, Zahratul Aisy, Laaili Azti Rohaadatul Akhiruddin Maddu Ali Aulia Ghozali Alif, Alfiah Aliya Nur Hasanah Amalyah Febryanti Amalyah Febryanti Anas Miftah Fauzi Ardiati, Fenny Clara Bakar, N.H.H Abu Boybul Boybul Charlena Dede Heri Yuli YANTO Dyah Iswantini Fitriani Indahsari Fretty Yurike Gufron Wahyudi Ida Ayu Putu Sri Widnyani Inda Iliyin Iqbal, Anwar Koesnadi Lee Wah Lim Lisa Aprilia Indriyani Lisa Aprilia Indriyani Maulidina, Ahsani Mohamad Rafi Mohamad Rafi Muhammad Fathurrahman Naser, Inda Hastuti N Noerpitasari, Erlina Nor Pana Yupa Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Nurhayat, Oktan Dwi Nurul Shofiah Peni Puspitasari, Peni Purwantiningsih Sugita Putera, Zulmy Faqihuddin Raharjo, Rahmawati Rizki Putri Ramadhani, Rizki Putri Roza Linda Roza Linda Sandu Siyoto, Sandu Setyanto T. Setyanto T., Setyanto Sholahudin, M. Fiqri Sulistioso G.S Sulistioso G.S, Sulistioso SUMINAR SETIATI ACHMADI Suryanegara, Lisman Susanti Susanti Tetty Kemala Tina Rosdiana Triesye Utari Tun Tedja Irawadi Utari, Triesye Wahyudi Priyono Suwarso Widajanti Wibowo Widajanti Wibowo Yanlinastuti Yanlinastuti Zainal Alim Mas’ud