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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa ILMU KELAUTAN: Indonesian Journal of Marine Sciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter ASPIRATOR Jurnal Perikanan dan Kelautan BIOMA : Jurnal Biologi Makassar Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Journal of Parasite Science Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine Jurnal Agripet
Risa Tiuria
Divisi Parasitologi Dan Entomologi Kesehatan, Departemen Ilmu Penyakit Hewan Dan Kesmavet, Sekolah Kedokteran Hewan Dan Biomedis, Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Populasi Ascaridia galli Dalam Usus Halus Ayam Yang Diberikan Kombinasi Ekskretori/Sekretori L3 dan Imunoglobulin Yolk Darmawi Darmawi; Ummu Balqis; Risa Tiuria
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i2.370

Abstract

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.
Diagnostic case study and treatment of Giardiasis in cat at Rvet Clinic Bogor Risa Tiuria; Hardiyanti, Niken; Akbari, Rizal Arifin
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.22153

Abstract

Giardia sp. is a digestive tract protozoan that can cause Giardiasis. This disease is classified as a water-borne parasitic disease. Giardiasis infects cats, especially cats who have a habit of drinking raw water or sewage. This case study aims to learn the treatment given to cats with Giardiasis. The cat in this study was named Memed, a cat with a mixed Persian breed and was 1.5 years old. Memed came to the RVet Bogor clinic with a grievance of diarrhea without blood for six days and was not accompanied by vomiting. The owner explained the cat was often released and drank water carelessly. Memed was diagnosed with Giardiasis after discovering of Giardia cysts in stool samples that were observed using the fecal flotation technique. Memed was given medical therapy using Metronidazole and vitamin B12 preparations. After the 5th post-therapy day, Memed was declared cured of giardiasis, characterized by the consistency returning to normal and no Giardia cysts found in the feces. Keywords: Giardiasis, Cats, Fecal Flotation, Metronidazole
TREMATODE INFECTION IN GASTROINTESTINAL TRACT OF DUCK FROM CHICKEN SLAUGHTERHOUSES IN BOGOR, WEST JAVA Aviola, Lala; Tiuria, Risa; Nugraha, Budiman
Jurnal Kedokteran Hewan Vol 19, No 3 (2025): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i3.23945

Abstract

This study aims to identify and calculate the percentage of trematode infection in the gastrointestinal tract of ducks from chicken slaughterhouses in Bogor, West Java. This research used 10 ducks gastrointestinal tract, of which five came from chicken slaughterhouse in Bojong Gede and the rest came from a chicken slaughter house in Cibinong Market. The gastrointestinal tract was divided into four parts: the upper, middle and lower part of the intestine and cecum. Each feces sample was examined by making slides using native and floatation methods to identify the egg of endoparasite. Adult trematodes found in the sample were stained for genus identification. This study showed that 4 out of 10 ducks were positive for endoparasite infection. Trematodes found in this study were Echinostoma spp. (20%) along the small intestine, large intestine, and cecum. Echinostoma spp. egg was also found in this study another trematode genus found was Hypoderaeum spp. (10%) in the large intestine and cloaca.
Musim dan toxocariasis pada kucing di klinik hewan Rvet 1 Bogor Fikram, Muhammad; Tiuria, Risa; Akbari, Rizal Arifin
ARSHI Veterinary Letters Vol. 7 No. 2 (2023): ARSHI Veterinary Letters - Mei 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.2.33-34

Abstract

Toxocara spp. merupakan cacing nematoda parasitik zoonotik yang dapat menginfeksi kucing. Studi kasus ini bertujuan untuk mengetahui jumlah pasien kucing toxocariasis di klinik hewan Rvet 1 Bogor pada musim hujan dan kemarau. Pasien kucing sebanyak 41 ekor 2581 pasien yang mendapatkan layanan medis menunjukkan terinfeksi Toxocara spp. Kasus toxocariasis tertinggi (2,01%) terjadi pada musim hujan periode bulan Oktober-Maret yaitu 24 dari 1193 pasien, sedangkan jumlah kasus toxocariasis terendah (1,22 %) pada musim kemarau periode bulan April-September yaitu 17 dari 1388 pasien.
Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli Balqis, Ummu; Tiuria, Risa; Priosoeryanto, Bambang Pontjo; D, Darmawi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3129

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380g of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehosts defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D.; Pasaribu, Fachriyan H.
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3122

Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
The Development of Ascaridia galli Infective Eggs by In Vitro Culture Balqis, Ummu; D, Darmawi; Hambal, Muhammad; Tiuria, Risa
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.3104

Abstract

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli, embrionated eggs
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D; Pasaribu, Fachriyan H
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3074

Abstract

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100 ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 510 L3 dikultur secara in vitro dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 g ml-1 streptomisin, 5 g ml-1 gentamisin dan 0,25 g ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.
ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA Mirsageri, M.; Assidiqi, M. Jamaluddin; Cahyaningsih, Umi; Tiuria, Risa; Z, Zulfiqri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2796

Abstract

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedoeno, Retno Damayanti; Pasaribu, Fachriyan Hasymi; Hambal, Muhammad; Daud, Razali
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 g ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 g ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,631,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,510,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
Co-Authors . Darmawi . Darmawi Adhi Rahmat Hariyadi Adi Winarto Afiff , Usamah Agus Setiyono Akbari, Rizal Arifin Alif Nur Muhamad Muriana April Hari Wardhana Arif Rahman Jabal Arini Ratnasari Aurilia Hemas Adytia Wardaningrum Aviola, Lala Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Chairun Nisa Cut Aldila Febiana Damiana Rita Ekastuti Darmawi D Darmawi D Deri Kermelita Dewantoro, Galuh Indro Didit Triatmojo Dita Pratama Putri Dyah Haryuningtyas Savitri Elok Budi Retnani Elsharkawy, Sara Fachriyan H Pasaribu Fachriyan H Pasaribu Fachriyan H. Pasaribu Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Feri Irawan Fikram, Muhammad Fitri Dinar Rahayu Forcep Rio Indaryanto Forcep Rio Indaryanto Forcep Rio Indaryanto Hadri Latif Hardiyanti, Niken HERA MAHESHWARI hubullah fuadzy Huda Salahudin Darusman Inna Rakhmawati Ismah Atika Salmah Jerica, Monica Silva Jimmy Pangihutan Kusuma, Nurlita Indah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Maria Natasya Mayasari, Ni Luh Putu Ika Merlia Andriyani Mila Karmila Milda Lestari Mohammad Mukhlis Kamal Muhammad Hambal Muhammad Raihan Pambudi Noviara, Sherly Nugraha, Arifin Budiman Nugraha, Budiman Nurlisa Alias Butet Nursa Rima Putri Putri Indah Ningtias Razali Daud Razali Daud Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno D. Soejoedono Retno Damayanti Soejoedono Ridi Arif Ridwan Affandi Ripta Mustafa Nugraha Riyan Wahyu Setiadi Rizal Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizky Diyu Purnama Samadi Samadi Siagian, Tetty Barunawati Simon He Sonja Kleinertz Subangkit, Mawar Sulistiono Suryaningtyas Kusumadewi Susi Soviana Tri Isyani Tungga Dewi Umi Cahyaningsih Ummu Balqis Unita Pratiwi Upik Kesumawati Hadi Vici E.H.S. Susilowati Yusli Wardiatno Yusuf Ridwan Yusuf, Muhammad Baharudin ZK Abdurahman Baizal Zulfikri Mustakdir Zulfiqri Z Zulfiqri Z, Zulfiqri