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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa ILMU KELAUTAN: Indonesian Journal of Marine Sciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter ASPIRATOR Jurnal Perikanan dan Kelautan BIOMA : Jurnal Biologi Makassar Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Journal of Parasite Science Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine Jurnal Agripet
Risa Tiuria
Divisi Parasitologi Dan Entomologi Kesehatan, Departemen Ilmu Penyakit Hewan Dan Kesmavet, Sekolah Kedokteran Hewan Dan Biomedis, Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Gambaran kepekaan Escherichia coli dari peternakan ayam broiler di Desa Bojongkerta Kabupaten Sukabumi terhadap antibiotik Merlia Andriyani; Usamah Afiff; Risa Tiuria
ARSHI Veterinary Letters Vol. 4 No. 1 (2020): ARSHI Veterinary Letters - Februari 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.934 KB) | DOI: 10.29244/avl.4.1.19-20

Abstract

Masalah resistensi Escherichia coli (E. coli) terhadap antibiotik telah banyak dilaporkan. Peningkatan kasus resistensi bakteri terhadap antibiotik dapat dilihat dari banyaknya laporan bahwa terdapat antibiotik yang sudah tidak efektif lagi terhadap suatu bakteri patogen. Pola penggunaan antibiotik di Indonesia dan negara-negara lain semakin meningkat dan banyak diantaranya masih digunakan secara tidak tepat. Tujuan penelitian ini adalah mempelajari gambaran kepekaan E. coli dari ayam broiler terhadap sefpodoksim, sefoksitin, sulfametoksazol-trimetoprim, kolistin sulfat, seftizoksim, aztreonam, dan nitrofurantoin dari peternakan ayam broiler di Desa Bojongkerta Kabupaten Sukabumi Jawa Barat. Pengujian resistensi antibiotik menggunakan metode difusi cakram menurut Kirby-Bauer. Hasil uji kepekaan isolat E. coli menunjukkan bahwa isolat E.coli dalam penelitian ini telah resisten terhadap sefpodoksim, sefoksitin, sulfametoksazol-trimetoprim, seftizoksim, aztreonam, dan nitrofurantoin. Namun tidak menunjukkan hasil terhadap kolistin sulfat.
Uji resistensi Escherichia coli dari peternakan ayam ras petelur di Desa Rumpin Kabupaten Bogor terhadap antibiotik Nursa Rima Putri; Usamah Afiff; Risa Tiuria
ARSHI Veterinary Letters Vol. 4 No. 2 (2020): ARSHI Veterinary Letters - Mei 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.4.2.35-36

Abstract

Escherichia coli merupakan flora normal yang ada di usus manusia dan hewan. Antibiotik berperan sebagai terapi terhadap infeksi E. coli. Penggunaan antibiotik secara tidak bijak dapat menimbulkan resistensi. Tujuan penelitian ini adalah mengukur tingkat resistensi E. coli yang diisolasi dari ayam ras petelur terhadap sefpodoksim, sefoksitin, seftizoksim, aztreonam, oksasilin, nitrofurantoin, dan sulfametoksazol−trimetoprim. Sampel uji berasal dari swab kloaka ayam ras petelur. Pengujian resistensi E. coli terhadap antibiotik dilakukan dengan metode difusi agar. Hasil yang diperoleh kemudian dibandingkan dengan Clinical Laboratory Standards Institute. Hasil penelitian menunjukkan bahwa 17 isolat E. coli mengalami resistensi terhadap sefpodoksim (100.0%), sefoksitin (58.82%), seftizoksim (100.0%), aztreonam (94.12%), oksasilin (100.0%), nitrofurantoin (58.82%), dan sulfametoksazol−trimetoprim (82.35%).
Kecacingan pada saluran pencernaan monyet ekor panjang (Macaca fascicularis) Rizky Diyu Purnama; Risa Tiuria; Huda Salahudin Darusman
ARSHI Veterinary Letters Vol. 5 No. 2 (2021): ARSHI Veterinary Letters - Mei 2021
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.2.37-38

Abstract

Monyet ekor panjang (Macaca fascicularis) merupakan satwa primata yang sering ditangkarkan dan digunakan sebagai hewan coba terutama dalam pengujian vaksin dan obat. Salah satu gangguan kesehatan yang perlu diantisipasi dalam upaya penangkaran adalah kecacingan. Penelitian ini bertujuan untuk mengetahui jenis telur cacing dan derajat infeksi kecacingan pada monyet ekor panjang. Sampel feses dari 26 monyet ekor panjang diambil dan dianalisa meggunakan metode McMaster dan metode Kato-Katz. Hasil pemeriksaan menunjukkan 4 sampel feses ditemukan telur cacing Trichuris Sp. dan Strongylid dengan kategori derajat infeksi ringan
Aspiculuris tetraptera Infection In Mice: Parasite degree and Differential Leukocyte Zulfikri Mustakdir; Rizal Arifin Akbari; Elok Budi Retnani; Risa Tiuria
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No 2, JULY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i2.20579

Abstract

Aspiculuris tetraptera is one of helminth parasite commonly found in mice. The response of the mice to parasite infection varies depending on various factors. Immune system is one of the factors that affect the occurrence of parasite infection in the host. The purpose of this study were to observe the parasitemia level and the immune respons of mice that infected with Aspiculuris before and after challenge infection. Mice were infected with Aspiculuris twice to see the development of parasite and the immune respon. Feces and blood samples were collected from the mice. Feces samples were examined by floating method to find the parasite egg, whereas blood samples were examined by blood smears to count the differential leukocytes. The result showed that Aspiculuris tetraptera eggs appear in the feces on the 20th day after infected as many 200 eggs/gram and increased on the 26th day as much 300 eggs/gram of feces. The peaks of eosinophils were on the 3rd and 6th days at 2.8% and 3.1% respectively. The level of Neutrophils and monocytes showed fluctuations but the trend decrease from day to day. Lymphocyte levels also showed fluctuations but tended to increase from the first day to the 26th day.
The Development of Ascaridia galli Infective Eggs by In Vitro Culture Ummu Balqis; Darmawi D; Muhammad Hambal; Risa Tiuria
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.372 KB) | DOI: 10.21157/j.ked.hewan.v3i2.3104

Abstract

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli,  embrionated eggs
ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA M. Mirsageri; M. Jamaluddin Assidiqi; Umi Cahyaningsih; Risa Tiuria; Zulfiqri Z
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.462 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2796

Abstract

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.
Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli Ummu Balqis; Risa Tiuria; Bambang Pontjo Priosoeryanto; Darmawi D
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.92 KB) | DOI: 10.21157/j.ked.hewan.v1i2.3129

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost’s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno Damayanti Soejoedoeno; Fachriyan Hasymi Pasaribu; Muhammad Hambal; Razali Daud
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.888 KB) | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno D. Soejoedono; Fachriyan H. Pasaribu
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.081 KB) | DOI: 10.21157/j.ked.hewan.v1i2.3122

Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno D Soejoedono; Fachriyan H Pasaribu
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (863.975 KB) | DOI: 10.21157/j.ked.hewan.v3i1.3074

Abstract

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein  ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100  ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 5–10  L3 dikultur secara in vitro  dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 µg ml-1 streptomisin, 5 µg ml-1 gentamisin dan 0,25 µg ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian  menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.
Co-Authors . Darmawi . Darmawi Adhi Rahmat Hariyadi Adi Winarto Afiff , Usamah Agus Setiyono Akbari, Rizal Arifin Alif Nur Muhamad Muriana April Hari Wardhana Arif Rahman Jabal Arini Ratnasari Aurilia Hemas Adytia Wardaningrum Aviola, Lala Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Chairun Nisa Cut Aldila Febiana Damiana Rita Ekastuti Darmawi D Darmawi D Deri Kermelita Dewantoro, Galuh Indro Didit Triatmojo Dita Pratama Putri Dyah Haryuningtyas Savitri Elok Budi Retnani Elsharkawy, Sara Fachriyan H Pasaribu Fachriyan H Pasaribu Fachriyan H. Pasaribu Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Feri Irawan Fikram, Muhammad Fitri Dinar Rahayu Forcep Rio Indaryanto Forcep Rio Indaryanto Forcep Rio Indaryanto Hadri Latif Hardiyanti, Niken HERA MAHESHWARI hubullah fuadzy Huda Salahudin Darusman Inna Rakhmawati Ismah Atika Salmah Jerica, Monica Silva Jimmy Pangihutan Kusuma, Nurlita Indah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Maria Natasya Mayasari, Ni Luh Putu Ika Merlia Andriyani Mila Karmila Milda Lestari Mohammad Mukhlis Kamal Muhammad Hambal Muhammad Raihan Pambudi Noviara, Sherly Nugraha, Arifin Budiman Nugraha, Budiman Nurlisa Alias Butet Nursa Rima Putri Putri Indah Ningtias Razali Daud Razali Daud Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno D. Soejoedono Retno Damayanti Soejoedono Ridi Arif Ridwan Affandi Ripta Mustafa Nugraha Riyan Wahyu Setiadi Rizal Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizky Diyu Purnama Samadi Samadi Siagian, Tetty Barunawati Simon He Sonja Kleinertz Subangkit, Mawar Sulistiono Suryaningtyas Kusumadewi Susi Soviana Tri Isyani Tungga Dewi Umi Cahyaningsih Ummu Balqis Unita Pratiwi Upik Kesumawati Hadi Vici E.H.S. Susilowati Yusli Wardiatno Yusuf Ridwan Yusuf, Muhammad Baharudin ZK Abdurahman Baizal Zulfikri Mustakdir Zulfiqri Z Zulfiqri Z, Zulfiqri