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All Journal Jurnal Sains Materi Indonesia Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease MEDISAINS Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Chemistry Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology Indonesian Community Journal Prosiding Seminar Nasional Unimus JURNAL REKAYASA KIMIA & LINGKUNGAN

Prihartini Widiyanti

Biomedical Engineering Study Program, Department Of Physics, Faculty Of Science And Technology, Universitas Airlangga Institute Of Tropical Disease, Universitas Airlangga

Author-ID : 508655

Agriculture, Biological Sciences & Forestry Arts Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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THE EFFECT OF GENDARUSSIN A ISOLATES OF Justicia gendarussa Burm.f. LEAF IN REVERSE TRANSCRIPTASE IN HIBITION OF HIV TYPE I INVITRO E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.

PROFILE OF HEMATOCRIT LEVEL CAPTURED BY DIGITAL HEMATOCRIT TEST Widiyanti, Prihartini; Sardjono, Tri Arif
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

The dengue fever is a disease caused by Dengue virus which is transmitted via Aedes aegypti and Aedes albopictus vector. This Dengue Haemorrhagic Fever (DHF) case in Indonesia tend to rise from year to year caused by delayed detection and inadequate handling. The laboratory parameter of hematocrite had regularly been performed using invasive method by taking the blood from the patient. This method is still not been able to monitor patients with DHF by repetitive and accurate measurament. This research project aims is to perform a digital hematocrit test (DHT) with non-invasive accurate sensors. Digital Hematocrit Test (DHT) is needed to presenting fast, exact, economical and accurate detection methods of hematocrit level. Measureable magnitude by the instrumentation is non-absorb intensity electromagnetic waves 560 nm emitted by transmitter captured by receiver. Signal captured by the receiver then converted into electrical signal. The electrical signal from receiver was the levels of hemoglobin. Levels of hemoglobin then converted to hematocrit. Hematokrit is three times the level of hemoglobin. Technology of hematocrit monitoring is aimed to control DHF patient clinical symptoms continuously and acquisitively.

EFFECT OF FREE ALKALOID AND NON-FREE ALKALOID ETHANOL 70% EXTRACT OF JUSTICIA GENDARUSSA BURM F. LEAVES AGAINST REVERSE TRANSCRIPTASE HIV ENZYME IN VITRO AND CHEMICAL COMPOUND ANALYSIS Prajogo, Bambang; Widiyanti, Prihartini; Riza, Hafrizal
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

HIV-AIDS is a global problem and the deadliest disease in the world. One of HIV and AIDS prevention strategy can be done with traditional medicine research program from natural resource that has anti-HIV AIDS activity. It has been found that 70% ethanol extract of Justicia gendarussa Burm.f leaves, alkaloid free and alkaloid non-free, has a strong inhibitory activity against HIV reverse transcriptase enzyme, as an effort to find a solution in the face of HIV AIDS prevalence that is still high with problem of HIV-AIDS treatment such as side effects and resistances. Justicia gendarussa had already known for having an effect anti-HIV and therefore we were looking at the mechanism of inhibition of HIV Reverse Transcriptase enzyme. Both types of extracts were tested in vitro using ELISA technique and analysed chemical content of Gendarusin A as anti-HIV using high performance liquid chromatography. ELISA test results obtained percent inhibition, respectively for 254.2, 254.2, 235.6, and 279.7 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of free alkaloid extract and 169.0, 164.0, 130.5 and 369.5 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of non-free-alkaloid extract. The results of high performance liquid chromatography obtained Gendarusin A in the free alkaloid extract at retention time 8.402 minutes and non-free alkaloid extract at retention time 8.381. Therefore, these results concluded that the Justicia gendarussa Burm.f can be a useful resource for the isolation and development of new anti-HIV.

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL Widiyanti, Prihartini; Prajogo, Bambang; Hikmawati, Ni Putu Ermi
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 Î¼g/ml and 78 Î¼g/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

ANALYSI S ON SECONDARY I NFECTI ON- TRI GGERI NG MICROORGANISMS IN HIV/AIDS PATIENTS AS A MODEL FOR POLICY CONTROL Rahayu, Retno Pudji; Nasronudin, Nasronudin; Indrawati, Retno; Widiyanti, Prihartini; Lukito, Bimo Dwi; Ferdiansyah, Ferdiansyah; Khairunisa, Siti Qomariyah; Mutamsari, Adiana; Kotaki, Tomohiro
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 4 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

HIV infection is associated with immune-compromised and rising in opportunistic infection (secondary infection). Therefore, the number of mortality caused by HIV/AIDS is increasing. The use of ARV and development of HIV/AIDS management are expected to suppress the progress of HIV infection into AIDS and, therefore, the mortality can be diminished, while in fact most of the patients eventually suffer from AIDS due to secondary infection that commonly causes death. There should be a management by analysing microorganisms that trigger secondary infection. The method of this study was observational descriptive with cross sectional design. HIV infected blood samples were using ELISA Antibody (IgG and IgM) and Polymerase Chain Reaction (PCR) on laboratory test. The result showed correlation between HIV/AIDS severity and the amount and types of secondary infection. The most common secondary infections were toxoplasm (96.77%), hepatitis C (22.58%), tuberculosis (19.35%), and hepatitis B (3.22%). Other less frequent secondary infections, which were quite difficult to diagnose and not commonly found in Indonesia, were West Nile Virus (25.81%), Japanese Encephalitis Virus (3.22%), and Enterovirus (3.22%). Due to MDGs (Millenium Development Goals) target and the results above, researchers are highly demanded to contribute in decreasing mortality related to AIDS through early detection of secondary infection,including type of infection which have not been commonly found in Indonesia, such as West Nile Virus and Nipah Virus. The discovery of secondary infection in this study was not enough to suppress the occurrence of infection in HIV/AIDS patients. Antimicrobes and good nutrition are required. Moreover, there should be either a primary or secondary prophylaxis to prevent secondary infection that raises the number of mortality and morbidity of HIV/AIDS patients. The result of this study was to meet the target of MDGs by establishing new policies in handling HIV/AIDS infections and have potential as model for policy control in HIV/AIDS.

A Patient with Suspected Diphtheria Arfijanto, Muhammad Vitanata; Mashitah, Siti Irma; Widiyanti, Prihartini; Bramantono, Bramantono
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 2 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

It was reported that a mature woman, Mrs. S, 42 years old with several complaints and symptoms such as fever, swallowing painweak body, swollen tonsil with beslag, dirty uvula of mouth cavity and tongue, and bullneck. The final diagnosis indicated that thepatient was suspected diphtheria, candidiasis oris, sepsis, and pneumonia. The sudden death of the patient was probably caused bymyocarditis.

The Role of Hyperbaric Oxygen to Platelet Aggregation in Diabetic Patients Type II (NIDDM) Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 2 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Prevalency of Diabetes Mellitus in Indonesia has tendency to be increased from year to year. Hyperbaric Oxygenation (HBO) has been used as treatment of Diabetes Mellitus's complication especially diabetic gangrene. But the effect of HBO to the rheology's disfunction especially platelet's aggregation in the patients of NIDDM was investigated. The randomized pretest-posttest design was used in this study. An experimental laboratory study was performed at Naval Health Institution in Surabaya. 32 patients of NIDDM,women, 40–75 years old, normal physic's diagnosa, normal thorak's photo, normal EKG, normal Ear Nose and Throat, Diabetes Mellitus's family's record, normal weight (BMI), blood glucose level didn't exceed 400 mg/dl (including controlled DM FBG < 120 mg/dl dan 2 H BG < 160 mg/dl), NIDDM, normal level of HbA1c (4–5,9), as long as this research they couldn't take their Oral Hipoglikemic Agent, Oral Anti Trombotic, vitamin C and vitamin E. They are divided into 2 group: group of HBO 100% O 2,4 ATA for 3x30 minutes with interval 5 minutes to inhalate air once a day daily for 5 days subsequently and the extraction of data (PAT) had been held before oxygenation hyperbaric therapy at the first day and the end of fifth days, in control group only giving 20% O2 with the pressure 1 ATA for 90 minutes once a day daily for 5 days subsequently and the extraction of data (PAT) had been held before normoxia normobaric therapy at the first day and the end of fifth days. The results were significant decrease of the platelet's aggregation level especially percent of aggregation after 5 days from 76,56 ± 8,06 become 69,13 ± 6,03. Latent Periode has also decrease from 28,75 ± 3,87 become 25,75 ± 2,82. Speed of Aggregation has also decrease from 66,25 ± 3,17 become 62,50 ± 3,44. Index of Aggregation has also decrease from 0,763 ± 0,071 become 0,581 ± 0,083. Using paired t-test, it could be seen the decrease of Latent Periode (p= 0,001) and index of aggregation (p= 0,000) significantly after exposure of oxygenation hyperbaric HBO 2,4 ATA 100% O 3í—30 minutes with interval 5 minutes inhalate air once a day for 5 days,subsequently. Speed of aggregation (p = 0,022) and percent of aggregation (p= 0,013) are nonsignificantly. The conclusion of this research is that oxygenation hyperbaric 2,4 ATA 100% O2 3í—30 minutes with interval 5 minutes inhalate air once a day for 5 days,subsequently could decrease latent periode, speed of aggregation, index of aggregation and percent of aggregation in NIDDM

CLINICAL MANIFESTATION APPROACH OF DENGUE VIRAL INFECTION Tjahjono, Ganis; Widiyanti, Prihartini; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 2 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Currently by an estimated 50-100 million dengue fever cases per year in worldwide, 500.000 were in the form of a disease is heavy Dengue Hemorraghic Fever (DHF) and Dengue Shock Syndrome (DSS). Survey serology in Indonesia show that DEN-1 and DEN-2 are the dominant serotipe virus until the end of the 1980s but the recent shift has occurred epizoötic where viruses DEN-3 dominant. (Dos Santos, 2004; Malavige, 2004; Stephenson, 2005). Dengue virus infection induces transient immune aberrant activation of CD4/CD8 ratio inversion and cytokine overproduction, and infection of endothelial cells and hepatocytes causes apoptosis and dysfunction of these cells. The aberrant immune responses not only impaire the immune response to clear the virus, but also result in overproduction of cytokines that affect monocytes, endothelial cells, and hepatocytes. Dengue-virus-induced vasculopathy and coagulopathy must be involved in the pathogenesis of hemorrhage, and the unbalace between coagulation and fibrinolysis activation, and prolonged duration of shock increase the likelihood of severe hemorrhage in DHF/DSS. Capillary leakage is triggered by the dengue virus itself or by antibodies to its antigen. To date, there are no effective strategies to prevent the progression of DHF/DSS. The control of dengue will be possible only after an efficient vaccine has been developed.

Polyvinyl Chloride (PVC)-Glycerol with Chitosan Addition for Antibacterial Blood Bag Application Widiyanti, Prihartini; Tarissa Diandra Putri Wibowo; Baskoro, Andhi; Siswanto
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 2 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i2.43104

Blood bag is a medical device that stokes and transports whole blood or blood components. The material that is often used for blood bag membranes is Polyvinyl Chloride (PVC), however the common problem that is bacterial contamination and that material have not antibacterial characteristic. Because of this matter, the aim of this reseach are a blood bag that has antibacterial function is needed and meet the ideal standard as bloodbag. Chitosan as a blood bag membrane material fabrication to get the antibacterial effect. Chitosan is chosen as a blood bag material fabrication to get the antibacterial effect. Chitosan has several specific biocompatibility properties, antibacterial, chelation, and biodegradability. This study used various Chitosan concentrations of 1.5%, 2%, 2.5%, and 3%, and Glycerol was added as a plasticizer. The composition of Chitosan: Glycerol is 1:1. Then, the mixture is added to the PVC solution in a ratio of 1:5 then poured into a petri dish. The results showed characterization that the biocomposite PVC-Glycerol with the addition of 3% concentration of chitosan was the best composition, the tensile strength test result of biocomposite is 21.20 MPa, the absence of membrane pores in the morphology of the blood bag, the hemolytic activity is 0.24%, and the inhibition zones of E. coli and S. aureus, respectively 11.66 mm and 12.66 mm in diameter. Based on the characterization results, the biocomposite PVC-Glycerol membrane with the addition of Chitosan has a very high potential as a candidate for blood bag membranes

Nigella sativa 3% Inhibition Test of NÐ°turÐ°l ToothpÐ°ste CompÐ°red Cetylpyridinum chloride (CPC) ToothpÐ°ste 0.01-0.1% on ÐggregÐ°tibÐ°cter actinomycetemcomitÐ°ns Setiawatie, Ernie Maduratna; Widiyanti, Prihartini; Retno Puji Rahayu; Eprilina, Alifiani; Sandrasari, Desi; Savitri, Irma Yosephina; Bargowo, Lambang; Rihandhita Shinta Meiliana
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 3 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i3.46075

Periodontitis generÐ°lly begins with gingivitis progresses to alveolar bone increÐ°sing the risk of systemic diseÐ°se. The primÐ°ry etiologicÐ°l fÐ°ctor in the etiology of periodontÐ°l diseÐ°se is ÐggregÐ°tibÐ°cter Ð°ctinomycetemcomitÐ°ns bacteria. Cetylpyridinium chloride (CPC) has a bactericidal effect by disrupting the function of bacterial membranes in the cytoplasm. CPC can also absorb negative charges from bacteria, increase bacterial cell wall permeability, decrease cell metabolism, and reduce bacterial attachment to teeth. Use of Ð°ntimicrobiÐ°l toothpÐ°ste, such as Cetylpyridinium chloride (CPC), is one strategy to prevent periodontÐ°l disease, but CPC is hÐ°zÐ°rdous in some quÐ°ntities. Ðs Ð° result, it should be compared to a natural toothpaste, specifically NigellÐ° sÐ°tivÐ° toothpÐ°ste. The objective of this study is to compare the Ð°ntibÐ°cteriÐ°l Ð°ctivity of nÐ°turÐ°l toothpÐ°ste contÐ°ining NigellÐ° sÐ°tivÐ° 3% with toothpÐ°ste contÐ°ining CPC 0.01% - 0.1% on ÐggregÐ°tibÐ°cter Ð°ctinomycetemcomitÐ°ns. ExperimentÐ°l studies are used in this kind of research. The colony count method wÐ°s used to Ð°ssess the nÐ°turÐ°l toothpÐ°stes NigellÐ° sÐ°tivÐ° 3% Ð°nd toothpÐ°ste contÐ°ining CPC 0.01- 0.1% for their cÐ°pÐ°city in inhibiting the ÐggregÐ°tibÐ°cter Ð°ctinomycetemcomitÐ°ns. Natural toothpÐ°ste containing NigellÐ° sÐ°tivÐ° 3% wÐ°s completely inhibiting ÐggregÐ°tibÐ°cter Ð°ctinomycetemcomitÐ°ns, compared to 0.01-0.1% CPC toothpÐ°ste. The significÐ°nce level for the stÐ°tisticÐ°l test results wÐ°s 0.000 (p<0.05). The conclution of this research are the nÐ°turÐ°l toothpÐ°ste contÐ°ining NigellÐ° sÐ°tivÐ° 3% Ð°nd toothpÐ°ste contÐ°ining CPC 0.06%–0.1% can effectively suppress the growth of the microorgÐ°nisms ÐggregÐ°tibÐ°cter Ð°ctinomycetemcomitÐ°ns.

Co-Authors - Aminatun - Siswanto Achmad Ardianto, Achmad Adanti Wido Paramadini Adiana Mutamsari Witaningrum Adiana Mutamsari, Adiana Adri Supardi Agung Prabowo Ahmad Fadli Akfiny Hasdi Aimon Akhmad Jayadi Alfian Pramudita Putra Aminatun Amma, Fadli Anggraini Barlian Anika Rahma Putri Arie Wibowo Azizia Kanya Fathiarachman Bambang Prajogo Baskoro, Andhi Bella Wahyu Sukma Wati Bimo Aksono Bimo Dwi Lukito, Bimo Dwi Bramantono Bramantono, Bramantono Budi Utomo Chandra Satria Arisgraha, Franky Cindy Narita Kusuma Astuti Deni Noviana Desi Sandrasari Dio Nurdin Setiawan, Dio Nurdin Dita Ayu Mayasari, Dita Ayu Djony Izak Rudyardjo Dyar Sasmito, Dyar Eko Budi Kuntjoro, Eko Budi Endah Purwanti Eprilina, Alifiani Ernie Maduratna Setiawatie Fathan Aditya Sanjaya Fatkhunisa Rahmawati, Fatkhunisa Fa’ilal Chusna Fendy Purwanda, Fendy Ferdiansyah Ferdiansyah Firdhausi Wardhani, Inten Fitriyatul Qulub Franky Arisgraha, Franky Franky Chandra Satria Arisgraha Gadis Meinar Sari Gadis Meinar Sari Hafrizal Riza, Hafrizal Hariyono Helmi Majid Ar Rasyid Heni Ismawati Heri Purnobasuki, Heri Herry Wibowo Herry Wibowo Indrio, Ludita Woro Isfandiary, Andini Jabbar, Hajria Jan Ady Jan Ady Julieta Kartikasari Juniastuti Juniastuti Khoirunnisa Suhandarini Kholifatul Wanda Putri, Kholifatul Wanda Khusnul Ain Kirana Anugerah Putri Lambang Bargowo M. Qushay, M. Maria Inge Lusida Maulida, Hendita Nur Mirzaq Hussein Anwar, Mirzaq Hussein Muhammad Bagas Ananda Muhammad Vitanata Arfijanto, Muhammad Vitanata Nabila Safira Khairina Nasronudin Nasronudin Nastiti, Helena Putri Ni Putu Ermi Hikmawati, Ni Putu Ermi Nilna Faizah Fiddarain, Nilna Faizah Nur Rahma, Osmalina Pramudita, Alfian Putri, Dina Kartika Radicha Arnanda Putri Indrawati Rehman, Saifur Retna Apsari Retno Indrawati Roestamadji Retno Pudji Rahayu Retno puji Rahayu Rihandhita Shinta Meiliana Riries Rulaningtyas Rozykulyyeva, Lale Satrio Adhitioso Savitri, Irma Yosephina Sherina Pradita Eka Wahyudi Shifa Fauziyah Siswanto Siswanto Siswanto Siti Irma Mashitah, Siti Irma Siti Qamariyah Khairunisa, Siti Qamariyah Siti Qomariyah Khairunisa, Siti Qomariyah Solly Aryza Suryani Dyah Astuti Tami Bachrurozy Tarissa Diandra Putri Wibowo Tasya Amalia Dwiyanti Teguh Hari Sucipto Tjahjono, Ganis Tomohiro Kotaki, Tomohiro Tri Arif Sardjono, Tri Arif Tria Refi Indriani Veren Audia Nurmansya Wijayanti, Tia Rahayu Wulandari Prima Devi Yufan Fibriawan, Yufan Yurituna Firda

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