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Hirowati Ali, Hirowati
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The Effect of Basil Leaves Extract (Ocimum basilicum) on The Triglyceride Level in Atherosclerosis Rats Atmi, Serly Rahma; Mahata, Liganda Endo; Rahmatini, Rahmatini; Rofinda, Zelly Dia; Ali, Hirowati; Aliska, Gestina; Khaira, Fathiyyatul
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.94222

Abstract

Atherosclerosis is the main cause of cardiovascular disease (CVD) characterized by fat accumulation and transformation, inflammation, smooth muscle cell proliferation, and necrotic cell remnants in the tunica intima of blood vessels. Basil leaves are known to contain active biochemical compounds that have the potential to reduce lipids. This is an experimental study that investigated the effect of basil leaves on rat triglyceride levels in atherosclerotic models. This research consisted of five groups of rats that were induced atherosclerosis by partial carotid ligation and a high-fat diet for 7 days. The treatment group was given basil leaves extract ethanol with 100 mg/kgBW, 200 mg/kgBW dose, and simvastatin 1,5mg/rat/day. Examination of triglyceride levels by enzymatic calorimetry GPO-PAP method using Micro Lab 300. The data were analyzed using One-way ANOVA and Post Hoc Bonferroni. The results showed that there was an average decrease in triglyceride levels in rats given basil leaves extract with 100 mg/kg BW dose and indicated that the Ocimum basilicum leaves extract affected triglyceride levels in rats induced by atherosclerosis.
Exploring the Effects of Bone Marrow Mesenchymal Stem Cells on Amyloid Plaque Reduction in a Rat Model of Alzheimer's Disease Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Majalah Kedokteran Andalas Vol. 47 No. 1 (2024): MKA Januari 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i1.p79-86.2024

Abstract

Objective: This study aimed to evaluate the therapeutic potential of Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) in mitigating amyloid plaque formation in Alzheimer's disease induced by Aluminum Chloride (AlCl3) in rats. Methods: Rats were divided into three groups: negative control, positive control (AlCl3-induced), and treatment (AlCl3 + BM-MSCs). Cognitive function was assessed five days post-induction, and amyloid plaques were quantified using Congo Red staining in the cortex and hippocampus. Results: A significant reduction in amyloid plaque was observed in rats treated with BM-MSCs. The proportion of stained areas decreased from 1.88 to 1.73 in the cortex and from 1.61 to 1.47 in the hippocampus, compared to AlCl3-only controls. Conclusion: BM-MSCs demonstrated a moderate but statistically significant reduction in amyloid plaque in a rat model of Alzheimer's disease, indicating their therapeutic potential.
Differences in Scube1 and Scube2 Gene Expression in Type II Diabetes Mellitus Patients with Dyslipidemia Saputri, Rahmi Agu; Ali, Hirowati; Tjong, Djong Hon; Anggraini, Debie; Yarni, Sisca Dwi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9875

Abstract

The Scube1 gene is expressed in atherosclerosis (plaque buildup in blood vessels), while Scube2 is expressed in DIT (Diffuse Intimal Thickening) which is used to describe diffuse thickening of the intima of blood vessels. This study aims to determine the differences in the expression of the scube1 gene in blood samples of type II DM patients with dyslipidemia and type II DM with non-dyslipidemia and differences in the expression of the scube2 gene in blood samples of type II DM patients with dyslipidemia and type II DM with non-dyslipidemia. With a comparative cross-sectional research method, the sample size of this study was determined based on the Lameshow hypothesis test formula which obtained 18 blood samples of type II DM with dyslipidemia, and 18 blood samples of type II DM with non-dyslipidemia. Blood samples of type II DM patients with dyslipidemia and blood samples of type II DM patients without dyslipidemia showed significantly different expression of the scube1 gene, with a p-value of 0.000. Similarly, blood samples from type II DM patients with dyslipidemia and those from type II DM patients without dyslipidemia showed a significant difference in scube2 gene expression (p = 0.001). Thus, it can be said that the expression of the Scube1 and Scube2 genes is significantly influenced by the complications of diabetes mellitus with dyslipidemia. This happens because it is believed that hyperglycemia in type II DM contributes to endothelial cell dysfunction, which occurs prior to blood vessel damage and results in progressively more severe blood vessel problems. Along with hyperglycemia, type II diabetes also results in dyslipidemia, an alteration in the normal lipid profile. Both dyslipidemia and hyperglycemia contribute to macrovascular and microvascular problems in type II diabetes.
Differential Levels and Biological Roles of eNOS and SCUBE1 in Endothelial Dysfunction: A Systematic Review Siregar, Muhammad Abi Ghoffari; Ali, Hirowati; Rahmadian, Rizki; Rasya, Salsabila Faiha Wiendra
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10226

Abstract

Endothelial dysfunction (ED) is a systemic disorder marked by impaired nitric oxide (NO) availability, which is essential for maintaining vascular tone and managing oxidative stress. This dysfunction contributes to vascular pathologies such as atherosclerosis and coronary artery disease (CAD) by fostering vasoconstriction, increased permeability, and inflammatory responses, thereby highlighting the urgent need for biomarkers and interventions aimed at mitigating endothelial dysfunction and its associated risks. The relationship between eNOS and SCUBE1 relates to endothelial function and oxidative stress. This study seeks to clarify the benefits and drawbacks of each biomarker in its capacity as a preventive strategy against endothelial dysfunction. This systematic review, adhering to the PRISMA Checklist, examines evidence on the role of endothelial function and oxidative stress in endothelial dysfunction, with emphasis on the biomarkers eNOS and SCUBE1. Literature searches were conducted in PubMed, ScienceDirect, and Google Scholar, and study quality was appraised based on reported biomarker values and relevance. There were 8 articles with increased eNOS and decreased ROS in several treatments, associated with prevention of endothelial dysfunction. There were 6 articles with increased SCUBE1 in several diseases (breast cancer, STEMI, hashimoto's thyroiditis, peripheral ischemic, pulmonary arterial hypertension, atherosclerosis) being markers of endothelial dysfunction. Both biomarkers can work well according to their release pathways, they can be novel biomarkers of endothelial dysfunction in various diseases. Their main role is on endothelial function, SCUBE1 controls BMPR2 signaling (angiogenic, proliferation, apoptosis) and eNOS focuses on endothelial health through NO production (vasodilation, maintaining vascular tone).
Efek Ekstrak Daun Kemangi (Ocimum Basilicum) Terhadap Kadar LDL Tikus (Rattus Norvegicus) Model Aterosklerosis Kencana, Gita; Ali, Hirowati; Noverial, Noverial; Kadri, Husnil; Mahata, Liganda Endo; Pertiwi, Dian
Innovative: Journal Of Social Science Research Vol. 5 No. 3 (2025): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v5i3.19552

Abstract

Penelitian ini bertujuan untuk mengetahui efek ekstrak daun kemangi (Ocimum basilicum) terhadap kadar LDL pada tikus (Rattus Norvegicus) model aterosklerosis. Desain yang digunakan adalah post-test only control group design dengan 30 ekor tikus jantan yang dibagi menjadi lima kelompok: kontrol negatif (K-), kontrol positif (K+), kontrol statin (KS), perlakuan I (P1; 100 mg/kgBB ekstrak daun kemangi), dan perlakuan II (P2; 200 mg/kgBB). Induksi aterosklerosis dilakukan melalui ligasi parsial arteri karotis dan pemberian diet tinggi lemak selama 7 hari. Pemberian simvastatin dan ekstrak kemangi dilakukan secara oral selama 7 hari. Kadar LDL diukur menggunakan metode CHOD-PAP dengan alat Microlab 300. Hasil analisis statistik menunjukkan perbedaan bermakna kadar LDL antara kelompok K-, KS, dan P2 dibandingkan dengan kelompok K+ (p<0,05). Rerata kadar LDL tertinggi ditemukan pada kelompok K+ (64,56 mg/dL) dan terendah pada kelompok KS (31,62 mg/dL). Dapat disimpulkan bahwa ekstrak daun kemangi, khususnya dosis 200 mg/kgBB, berpotensi menurunkan kadar LDL pada tikus model aterosklerosis.
Pengaruh Ekstrak Kemangi terhadap Ekspresi Gen Interleukin-1β pada Tikus Diabetes Melitus Gestasional Medison, Sultan Rafli Putra; Decroli, Eva; Ali, Hirowati
Jurnal Ilmu Kesehatan Indonesia Vol. 7 No. 1 (2026): Maret 2026
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v7i1.1464

Abstract

Latar Belakang: Istilah Diabetes Melitus Gestasional (DMG) mengacu pada adanya intoleransi glukosa yang ditemukan pertama kali pada kehamilan, menyebabkan terjadinya inflamasi yang ditandai dengan peningkatan ekspresi gen Interleukin-1β (IL-1β) dan dapat memicu terjadi stres oksidatif. Kemangi (Ocimum basilicum) merupakan tanaman herbal dengan kandungan zat aktif yang mempunyai efek antidiabetes, antiinflamasi maupun antioksidan. Objektif: Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak daun kemangi (Ocimum basilicum) terhadap ekspresi gen IL-1β pada tikus model diabetes melitus gestasional. Metode: Penelitian ini adalah eksperimental dengan menggunakan metode post test only control group design. Penelitian ini dilakukan di Laboratorium Biomedik Fakultas Kedokteran Universitas Andalas. Penelitian dilakukan selama bulan September 2023 sampai Januari 2024. Besar sampel yang digunakan ditentukan dengan rumus Federer. Jumlah sampel setiap kelompok adalah 6. Penelitian ini terdiri dari 4 kelompok yaitu 2 kontrol dan 2 perlakuan sehingga jumlah total sampel adalah sebanyak 24 serum. Hasil: Rerata hasil ekspresi gen IL-1β yang pada kelompok K- (kontrol negatif), K+ (kontrol positif), P1 (perlakuan 1), dan P2 (perlakuan 2) adalah masing masing 1,16, 1,24, 0,86, dan 0,81. Terdapat perbedaan bermakna pada setiap kelompok dengan nilai p = 0,000 (p<0,05). Hasil penelitian ini memperlihatkan  terdapat perbedaan bermakna penurunan ekspresi IL-1β antara K+ dengan P1 dan P2 (p<0,001) namun tidak terdapat perbedaan yang bermakna antara P1 dan P2. Kesimpulan: Pemberian ektrak kemangi dapat mempengaruhi ekspresi gen IL-1β pada tikus DMG.
Effect of Kawa Gambir (Uncaria gambir Roxb.) Leaves on Serum LDL in Diabetic Model Rats Siregar, Muhammad Abi Ghoffari; Rita, Rauza Sukma; Reza, Mohamad; Ali, Hirowati; Rofinda, Zelly Dia; Welan, Rahmani
Jurnal Kedokteran Meditek Vol 32 No 2 (2026): MARET
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v32i2.4012

Abstract

Background: Kawa gambir leaves, dried in a furnace and brewed as tea, are rich in flavonoids, phenolics, steroids, and tannins and have antioxidant, antihyperglycemic, and antidyslipidemic properties. In diabetes, elevated low-density lipoprotein (LDL) is a dyslipidemic abnormality that increases atherogenic risk. However, the effect of Kawa gambir on LDL in diabetic conditions remains unclear. Objective: To evaluate the effect of Kawa gambir leaf administration on LDL in alloxan-induced diabetic rats. Methods: This experimental study used a post-test-only control group design in 36 Wistar rats randomized into six groups: K−, K+, MET, P1, P2, and P3. Diabetes was induced with alloxan in K+, MET, and P1–P3. MET received metformin, whereas P1, P2, and P3 received Kawa gambir infusions at 1, 2, and 4 g/100 mL once daily for 4 weeks. Serum LDL was estimated using the Friedewald formula. Data were analyzed by one-way ANOVA followed by Least Significant Difference post hoc testing. Results: Mean±SD LDL levels (mg/dL) were 37.16±3.03, 59.90±8.91, 41.30±6.74, 40.60±5.75, 38.95±10.15, and 40.33±7.01 in K−, K+, MET, P1, P2, and P3, respectively. Group differences were significant (p=0.001). The largest numerical reduction versus K+ was found in P2, with a difference of 20.95 mg/dL (95% CI 8.64–33.26). Conclusion: Kawa gambir infusion significantly reduced LDL compared with the untreated diabetic control. Although the 2 g/100 mL group showed the lowest LDL numerically, no significant differences were observed among active treatment groups. These findings support Kawa gambir as an antidyslipidemic agent in diabetic dyslipidemia.
In Vitro Analysis of SCUBE1 Expression in Aortic Vascular Smooth Muscle Cells on Atherosclerosis Progression Rasya, Salsabila Faiha Wiendra; Ali, Hirowati; Yanni, Mefri; Rita , Rauza Sukma; Asri , Aswiyanti; Mahata , Liganda Endo; Putri , Biomechy Oktomalio; Yarni , Sisca Dwi
Jurnal Kedokteran Meditek Vol 32 No 3 (2026): MEI
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v32i3.4015

Abstract

Background:  Signal peptide-CUB-EGF domain-containing protein-1 (SCUBE1) is a platelet–endothelial glycoprotein implicated in thrombosis and vascular inflammation, and has been detected within atherosclerotic lesions. Whether vascular smooth muscle cells (VSMCs) upregulate SCUBE1 under inflammatory stimuli remains unclear. Objective:  To quantify SCUBE1 mRNA expression in rat aortic VSMCs following lipopolysaccharide (LPS) exposure in an organ-culture model of atherogenesis. Methods: This study aimed to analyze SCUBE1 expression in aortic VSMCs in atherosclerosis using real-time PCR. This study was a true experimental-post test control group design using organ culture. The aorta from six wistar rats were divided into two groups, consisting of five samples that were not induced with LPS and five samples exposed to LPS at a concentration of 10 µg/mL for 48 hours. Expression of the SCUBE1 gene was then analyzed using real-time PCR. The data was then processed using the dependent T-Test. Results: The result showed LPS increased SCUBE1 transcript levels compared with control (mean ± SD: 0.78 ± 0.22 vs 0.44 ± 0.35), representing a non-significant trend toward induction (p=0.07). These preliminary data are consistent with SCUBE1’s inducibility in inflammatory states and its presence in atherosclerotic tissue. Conclusion: This study revealed increased SCUBE1 gene expression in aortic VSMCs in the LPS-treated group. These data suggest that SCUBE1 has the potential to be a marker of endothelial dysfunction in atherosclerosis.
Co-Authors Aizah, Havina Nurul Almurdi Almurdi Amel Yanis Anggraini, Debie Annita ., Ansharina, Hafiznie Asri , Aswiyanti Atmi, Serly Rahma Basyir, Vaulinne , Beni Indra, Beni Cimi Ilmiawati, Cimi Desmawati Desmawati Dessy Arisanty Dian Pertiwi Dina Arfiani Rusjdi Djong Hon Tjong Eryati Darwin Eva Decroli Fathiyyatul Khaira, Fathiyyatul Febrina, Widia Finny Fitry Yani Gestina Aliska Gusti Revilla Hasmiwati Husnil Kadri Julita, Ema Kencana, Gita Lydia Aswati, Lydia Mahata , Liganda Endo Mahata, Liganda Endo Mahendika, Devin Mazara, Fitria Medison, Sultan Rafli Putra Muhammad Abi Ghoffari Siregar Muhammad Arif Nora Harminarti Noverial, Noverial Nur'aisyah, Shafira Putri , Biomechy Oktomalio Putri, Biomechy Oktomalio Putri, Nabila Priscilla Rahmadian, Rizki Rahmatini . Rasya, Salsabila Faiha Wiendra Rauza Sukma Rita Restu Susanti Reza, Mohamad Rita , Rauza Sukma Sahputra, Roni Eka Saputri, Rahmi Agu Silviam, Nelmi Siti Aisyah Sriyanti, Roza Sukri Rahman Tofrizal Wydya, Satya Yanni, Mefri Yarni , Sisca Dwi Yarni, Sisca Dwi Yusrawati Yusrawati Yusri, Elfira Zelly Dia Rofinda Zurayya Fadila