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All Journal Perspektif : Review Penelitian Tanaman Industri Elkawnie Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Pharmascience Journal of Islamic Pharmacy JPSCR : Journal of Pharmaceutical Science and Clinical Research Abditani : Jurnal Pengabdian Masyarakat SRIWIJAYA JOURNAL OF MEDICINE Medika Kartika : Jurnal Kedokteran dan Kesehatan Perspektif, Review Penelitian Tanaman Industri AJAD : Jurnal Pengabdian kepada Masyarakat Proceeding of Mulawarman Pharmaceuticals Conferences Medical Sains : Jurnal Ilmiah Kefarmasian Journal of Applied Agricultural Science and Technology Room of Civil Society Development Riset Informasi Kesehatan AGRIC kawanad Kartika : Jurnal Ilmiah Farmasi Medical Sains : Jurnal Ilmiah Kefarmasian Room of Civil Social Development Agroscript: Journal of Applied Agricultural Sciences
Fahrauk Faramayuda, Fahrauk
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Journal of Applied Agricultural Science and Technology

 1 
Comparison of Polyphenol Levels of Callus and Wild Type of Cat's Whiskers Plant ( Orthosiphon aristatus (Blume) Miq.) Purple Varieties Fahrauk Faramayuda; Soraya Riyanti; Ari Sri Widyaswari; Zaini Alfahmi; Sultan Salahudin Jamal; Totik Sri Mariani; Elfahmi Elfahmi; Sukrasno Sukrasno
Journal of Applied Agricultural Science and Technology Vol. 6 No. 2 (2022): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.608 KB) | DOI: 10.55043/jaast.v6i2.51

Abstract

The cat's whiskers plant ( Orthosiphon aristatus (Blume) Miq) that grows in Indonesia has white flowers, purple flowers, and white flowers with purple patterns. The quality of the secondary metabolite content of each cat's whiskers plant is different because it depends on environmental influences such as rainfall or soil mineral content. Plant tissue culture methods such as callus culture or cell culture are used to overcome these obstacles because these methods do not depend on the environment and can produce secondary metabolites such as those produced by the original plant. Cat's whiskers contain the main secondary metabolite compounds: rosmarinic acid, eupatorin, and sinensetin. Rosmarinic acid belongs to the phenolic group, while sinensetin and eupatorin are flavonoids. This study aimed to determine the total levels of polyphenols and flavonoids in purple cat whiskers and determine the ratio of secondary metabolites of cat whiskers callus with wild-type plants—determination of polyphenol content using Folin Ciocalteau reagent. Determining flavonoid levels is based on colorimetry using AlCl 3 reagent, specific for flavone and flavonol compounds. The levels of polyphenols contained in the ethanolic extract of callus and cat whiskers of the purple variety were 16.056 ± 0.204 mgQE/g and 30.780 ± 0.425 mgQE/g, while the polyphenol content of the callus ethyl acetate extract and the purple variety of cat whiskers leaves was 15,489 ± 0.425 mgQE/g and 13,262 ± 0.044 mgQE/g. The levels of flavonoids contained in the ethanol extract of the cat whiskers leaf were 1.39±0.03 mgQE/g and callus 0.16±0.02 mgQE/g in the ethyl acetate extract of the leaves of the cat whiskers 2.16±0.012 mgQE/g and callus 2.24±0.010 mgQE/g. The polyphenols and flavonoids in the callus ethyl acetate extract were more significant than the ethyl acetate extract of the purple variety ( wild type ) cat whiskers leaf.
Comparative Assessment of Antioxidant Properties Between In-Vitro Cultured Cat's Whiskers Leaves (Orthosiphon stamineus) and Quercetin Faramayuda, Fahrauk; Choirunnisa, Dinda; Karlina, Yenni
Journal of Applied Agricultural Science and Technology Vol. 9 No. 4 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i4.479

Abstract

The present study investigates the antioxidant properties of in-vitro cultured leaves of cat's whiskers (Orthosiphon stamineus) compared to the well-known antioxidant quercetin. Antioxidants are crucial for neutralizing free radicals and play a significant role in protecting cells from oxidative stress, a contributing factor to various diseases and aging processes. The research aims to quantify and compare the radical scavenging activity of extracts from the in vitro cultured leaves and quercetin, using the DPPH method, a widely recognized assay in antioxidant research. Extraction involved macerating in-vitro cultured cat's whiskers leaves with 96% ethanol, which facilitates the dissolution of active phytochemicals. Antioxidant activity was subsequently assessed through the DPPH assay, where colorimetric change indicates the extract’s scavenging ability towards free radicals. The findings revealed that the in vitro cultured leaves extract exhibited a potent antioxidant activity, with an IC50 value of 0.74 μg/mL, significantly lower than quercetin’s IC50 value of 7.51 μg/mL. These results highlight the potential of in-vitro cultured cat's whiskers as a natural antioxidant source, suggesting possible applications in the food and pharmaceutical industries, where combating oxidative stress is essential for health maintenance and disease prevention. Further research is necessary to elucidate the specific biochemical pathways and mechanisms underpinning the antioxidant capacity of the in vitro cultured leaves extracts.
Co-Authors Adella Shindy Pratiwi Adely, Alyaa Muliahati Adinda Fitriani Adriana, Reyhan Akbar, Tzazkia Febriyana Akhirul Kahfi Syam Amedea, Esanda Zulfi Ami Soega Dwigantina Ananda Tunjung Pertiwi Anggita Esa Putri Fitrichia Aprilia, Trialisa Ari Sr Windyaswari Ari Sri Widyaswari Ari Sri Windyaswari Ari Sri Windyaswari Ari Sri Windyaswari, Ari Sri Ayu, Inna Puspa Chandani Nurul Hafizah Choirunnisa, Dinda dessy ratnasari Dewi, Renti Mutiara Dhimas Ariya Wibiksana Dwi Ayu Pratiwi Dwigantina, Ami Soega Dyah Nur Azizah, Dyah Nur Elfahmi Elfahmi Elfahmi Elfahmi, Elfahmi Ema Mutya Endang Kumolowati Esanda Zulfi Amedea Euis Reni Yuslianti Faizal Hermanto Farhan Fikri Alatas Fitriani, Adinda Fitrichia, Anggita Esa Putri Iis Inayati Rakhmat Ismail, Nursafira Khairunnisa Jaka Permana Jovie Mien Dumanauw Julia Ratnawati Julia Ratnawati Khairunisa Harizqi Nurul Husna Limbong, Remare Halomoan Melani, Putri Dwi Muhamad Raihan Maulana Muhammad Irwan Mutya, Ema Niken Tunjung Murti Pratiwi Nira Purnamasari, Nira Nur Achsan Al-Hakim Nur Fauziyyah, Salma Alifia Nurhalizah, Dinda Siti Nurhayati, Viola Aditya Nursafira Khairunnisa Ismail Oktiyas Muzaky Luthfi Oktovia El Shara Paramita, Veronika Santi Pertiwi, Ananda Tunjung Pratiwi, Dwi Ayu Purnama, Uwan Purwoko, Agus Putri, Dhiffa Namira Alifia Ramelan, Riska Sigit Remare Halomoan Limbong Renti Mutiara Dewi Reyhan Adriana Deris Ridwan Ilyas Ridzka Magfirah Rifaz Muhammad Sukma Riska Sigit Ramelan Rizka Khoirunnisa Guntina Rizka Khoirunnisa Guntina RIZKA KHOIRUNNISA GUNTINA, RIZKA KHOIRUNNISA Septiani, Usi Sigid Pamungkas Wicaksono Silvy Julian Siti Muslichah Soraya Riyanti Soraya Riyanti Soraya Riyanti, Soraya Sufyan Zainul Arifin Sugiyanto Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sultan Salahudin Jamal SURYANI Suwidjiyo Pramono Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Tresa Tri Rayani Tresa Tri Rayani, Tresa Tri Trialisa Aprilia Tzazkia Febriyana Akbar Usi Septiani Viola Aditya Nurhayati Weni Widy Astuti Winda Nur Halimah Wulandari, Yesi Yeni Karlina Yeni Karlina Yeni Karlina, Yeni Yenni Karlina, Yenni Yesi Desmiaty, Yesi Yesi Wulandari Yuslianti, Euis Reni Zaini Alfahmi