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All Journal Perspektif : Review Penelitian Tanaman Industri Elkawnie Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Pharmascience Journal of Islamic Pharmacy JPSCR : Journal of Pharmaceutical Science and Clinical Research Abditani : Jurnal Pengabdian Masyarakat SRIWIJAYA JOURNAL OF MEDICINE Medika Kartika : Jurnal Kedokteran dan Kesehatan Perspektif, Review Penelitian Tanaman Industri AJAD : Jurnal Pengabdian kepada Masyarakat Proceeding of Mulawarman Pharmaceuticals Conferences Medical Sains : Jurnal Ilmiah Kefarmasian Journal of Applied Agricultural Science and Technology Room of Civil Society Development Riset Informasi Kesehatan AGRIC kawanad Kartika : Jurnal Ilmiah Farmasi Medical Sains : Jurnal Ilmiah Kefarmasian Room of Civil Social Development Agroscript: Journal of Applied Agricultural Sciences
Fahrauk Faramayuda, Fahrauk
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : AGRIC

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ANALISIS METABOLIT SEKUNDER DARI KALUS RAMBUTAN Nephelium lappaceum L Fahrauk Faramayuda; Elfahmi Elfahmi; Weni Widy Astuti
Agric Vol. 33 No. 1 (2021)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2021.v33.i1.p13-22

Abstract

Rambutan plant (Nephelium lappaceum L.) is a member of the Sapindaceae family. The rambutan plant is one of the natural ingredients that can be developed as traditional medicine. Rambutan peel has the potential for good antioxidant and anticancer activity. Rambutan fruit does not grow every time it needs efforts to produce the active substance in rambutan, using plant tissue culture techniques. The use of the correct variety of mediums and hormones at the right concentration is the key to thriving tissue culture. Explants derived from rambutan leaves were planted precisely on solid media Murashige and Skoog (MS) and WoddyPlant Medium (WPM) containing Indole-3-Butyric Acid (IBA) and Kinetin. After seven days, the callus was subcultured, then after 35 days, the subculture callus was collected and dried. Dry callus and rambutan leaves (Wild type) were macerated with n-hexane, ethyl acetate, and ethanol. The concentrated extract was then applied to a GF 254 silica gel plate with the mobile phase Toluene-Acetone (7: 3) and n-hexane-EthylAsetate (3: 7). The results showed that the concentration of IBA 2 ppm and kinetin three ppm was the best combination because it produced callus. TLC results of rambutan leave with plant tissue culture containing flavonoids and triterpenoids. This study provides new information regarding the induction of rambutan callus and can become the basis for producing active metabolites in rambutan with cell suspension culture development.
THE GROWTH OF PIMPINELLA ALPINA HOST CALLUS AT VARIOUS TREATMENTS OF PLANT GROWTH REGULATOR CONCENTRATIONS OF NAA. 2,4 D AND ITS COMBINATION WITH BAP Fahrauk Faramayuda; Muhammad Irwan; Akhirul Kahfi Syam
Agric Vol. 34 No. 2 (2022)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2022.v34.i2.p171-182

Abstract

Purwaceng (Pimpinella alpina) is an herbal plant that has aphrodisiac, diuretic, and tonic properties. The imbalance between the growth and the exploitation of this plant makes it included in Appendix I (endangered), so its conservation will be crucial. One method of the conservation of this plant is tissue culture. This method is considered one way to induct the callus. The research objective is to find the effect of various treatments of plant growth regulator concentrations of NAA. 2,4 D and its combination with BAP on the growth of purwoceng callus. Concentration of plant growth regulator NAA (0.5 mg L-1, 1 mg L-1, 2 mg L-1), 2,4-D (0.4 mg L-1, 0.8 mg L-1, 1.2 mg L-1 ), a combination of NAA with BAP (0.5 mg L-1 NAA: 1 mg L-1 BAP, 1 mg L-1 NAA: 2 mg L-1 BAP, mg L-1 NAA: 3 mg L-1 BAP) and a combination of 2, 4- D with BAP (1 mg LL 2,4-D : 0.5 mg L BAP, 1 mg L-1 2,4-D : 1 mg L-1 BAP, 2 mg L-1 2,4-D : 1 mg L-1 BAP) into Murashige Skoog medium. Then purwaceng leaf explants were planted on the medium until the growth was seen. This research shows that the plant growth regulator concentration has a different influence on the growth of callus that is recognized by the day of callus induction, colour and callus texture. MS media with growth regulator 1.2 mg L-1 2,4-D can grow callus faster (17 days) with friable texture.
Co-Authors Adella Shindy Pratiwi Adely, Alyaa Muliahati Adinda Fitriani Adriana, Reyhan Akbar, Tzazkia Febriyana Akhirul Kahfi Syam Amedea, Esanda Zulfi Ami Soega Dwigantina Ananda Tunjung Pertiwi Anggita Esa Putri Fitrichia Aprilia, Trialisa Ari Sr Windyaswari Ari Sri Widyaswari Ari Sri Windyaswari Ari Sri Windyaswari Ari Sri Windyaswari, Ari Sri Ayu, Inna Puspa Chandani Nurul Hafizah Choirunnisa, Dinda dessy ratnasari Dewi, Renti Mutiara Dhimas Ariya Wibiksana Dwi Ayu Pratiwi Dwigantina, Ami Soega Dyah Nur Azizah, Dyah Nur Elfahmi Elfahmi Elfahmi Elfahmi, Elfahmi Ema Mutya Endang Kumolowati Esanda Zulfi Amedea Euis Reni Yuslianti Faizal Hermanto Farhan Fikri Alatas Fitriani, Adinda Fitrichia, Anggita Esa Putri Iis Inayati Rakhmat Ismail, Nursafira Khairunnisa Jaka Permana Jovie Mien Dumanauw Julia Ratnawati Julia Ratnawati Khairunisa Harizqi Nurul Husna Limbong, Remare Halomoan Melani, Putri Dwi Muhamad Raihan Maulana Muhammad Irwan Mutya, Ema Niken Tunjung Murti Pratiwi Nira Purnamasari, Nira Nur Achsan Al-Hakim Nur Fauziyyah, Salma Alifia Nurhalizah, Dinda Siti Nurhayati, Viola Aditya Nursafira Khairunnisa Ismail Oktiyas Muzaky Luthfi Oktovia El Shara Paramita, Veronika Santi Pertiwi, Ananda Tunjung Pratiwi, Dwi Ayu Purnama, Uwan Purwoko, Agus Putri, Dhiffa Namira Alifia Ramelan, Riska Sigit Remare Halomoan Limbong Renti Mutiara Dewi Reyhan Adriana Deris Ridwan Ilyas Ridzka Magfirah Rifaz Muhammad Sukma Riska Sigit Ramelan Rizka Khoirunnisa Guntina Rizka Khoirunnisa Guntina Septiani, Usi Sigid Pamungkas Wicaksono Silvy Julian Siti Muslichah Soraya Riyanti Soraya Riyanti Soraya Riyanti, Soraya Sufyan Zainul Arifin Sugiyanto Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sultan Salahudin Jamal SURYANI Suwidjiyo Pramono Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Tresa Tri Rayani Tresa Tri Rayani, Tresa Tri Trialisa Aprilia Tzazkia Febriyana Akbar Usi Septiani Viola Aditya Nurhayati Weni Widy Astuti Winda Nur Halimah Wulandari, Yesi Yeni Karlina Yeni Karlina Yeni Karlina, Yeni Yenni Karlina, Yenni Yesi Desmiaty, Yesi Yesi Wulandari Yuslianti, Euis Reni Zaini Alfahmi