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All Journal Perspektif : Review Penelitian Tanaman Industri Elkawnie Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Pharmascience Journal of Islamic Pharmacy JPSCR : Journal of Pharmaceutical Science and Clinical Research Abditani : Jurnal Pengabdian Masyarakat SRIWIJAYA JOURNAL OF MEDICINE Medika Kartika : Jurnal Kedokteran dan Kesehatan Perspektif, Review Penelitian Tanaman Industri AJAD : Jurnal Pengabdian kepada Masyarakat Proceeding of Mulawarman Pharmaceuticals Conferences Medical Sains : Jurnal Ilmiah Kefarmasian Journal of Applied Agricultural Science and Technology Room of Civil Society Development Riset Informasi Kesehatan AGRIC kawanad Kartika : Jurnal Ilmiah Farmasi Medical Sains : Jurnal Ilmiah Kefarmasian Room of Civil Social Development Agroscript: Journal of Applied Agricultural Sciences
Fahrauk Faramayuda, Fahrauk
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Elkawnie

 1 
Identification Secondary Metabolites From Callus Piper retrofractum Vahl Fahrauk Faramayuda; Jaka Permana; Akhirul Kahfi Syam; Elfahmi Elfahmi
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.8630

Abstract

Abstract: Javanese chili (Piper retrofractum Vahl) is a traditional medicinal plant originating from Indonesia and has many pharmacological activities, one of which is often used as a base for aphrodisiac herbal medicine. The population of P. retrofractum is limited, so it is necessary to design secondary metabolite production and propagation efforts using plant tissue culture techniques. The materials used in this study were explants of P. retrofractum leaves that were induced in Murashige and Skoog (MS) media and the ratio of growth regulators 2.4-dichlorophenoxyacetis acid (2,4-D): Benzyl Amino Purine (BAP) 0.5: 0.5. The results showed that the callus of P. retrofractum was formed in the growth regulator 2.4D: BAP (0.5: 0.5). TLC and spectrophotometry identified the secondary metabolite content of callus. Secondary metabolite analysis using the thin layer chromatography (TLC) method using the mobile phase ethyl acetate: n-hexane (7: 3) showed a terpenoid compound indicated by purple spots on the visual appearance after spraying 10% spotting vanillin. Identification using infrared spectrophotometry shows functional groups -CH, C = O, C = C, -CH2, and -CH3, characteristic of terpenoid compounds. Based on TLC data and spectrophotometry, callus P. retrofractum is thought to contain terpenoid compounds. This study's results are expected to be the basis for developing secondary metabolite production in P. retrofractum with cell suspension culture and P. retrofractum propagation by micropropagation.Abstrak: Cabai Jawa (Piper retrofractum  Vahl) merupakan tanaman obat tradisional yang berasal dari Indonesia dan banyak memiliki aktivitas farmakologis salah satunya sering digunakan sebagai bahan dasar jamu afrodisiaka. Populasi tanaman cabai Jawa terbatas maka perlu dirancang upaya produksi metabolit sekunder dan upaya perbanyakan tanaman cabai Jawa salah satunya menggunakan teknik kultur jaringan tanaman. Bahan yang digunakan pada penelitian ini adalah eksplan daun tanaman cabai Jawa yang diinduksi pada media Murashige and Skoog (MS) dan perbandingan zat pengatur tumbuh 2.4-dichlorophenoxyacetis acid (2,4-D) : Benzyl Amino Purine (BAP) 0,5 : 0,5. Hasil penelitian menunjukkan bahwa kalus cabai Jawa terbentuk dalam zat pengatur tumbuh 2,4D: BAP (0,5: 0,5). Kandungan metabolit sekunder dari kalus diidentifikasi dengan KLT dan spektrofotometri. Analisis metabolit sekunder menggunakan metode kromatografi lapis tipis (KLT) menggunakan fasa gerak etil asetat: n-heksana (7: 3) menunjukkan adanya senyawa terpenoid yang ditunjukkan dengan adanya bercak ungu pada penampakan visual setelah disemprotkan spotting vanilin 10%. Hasil Identifikasi menggunakan spektrofotometri UV-Vis  menunjukkan isolat mempunyai panjang gelombang maksimum 272,6 nm. Identifikasi menggunakan spektrofotometri inframerah menunjukkan adanya gugus fungsi -CH, C = O, C = C, -CH2, dan -CH3 yang merupakan ciri khas senyawa terpenoid . Berdasarkan data KLT dan spektrofotometri kalus cabai Jawa diduga mengandung senyawa terpenoid. Hasil penelitian ini diharapkan dapat menjadi dasar pemgembangan produksi metabolit sekunder dalam tanaman cabai Jawa dengan kultur suspensi sel dan perbanyakan tanaman cabai Jawa dengan mikropropagasi.
Co-Authors Adella Shindy Pratiwi Adely, Alyaa Muliahati Adinda Fitriani Adriana, Reyhan Akbar, Tzazkia Febriyana Akhirul Kahfi Syam Amedea, Esanda Zulfi Ami Soega Dwigantina Ananda Tunjung Pertiwi Anggita Esa Putri Fitrichia Aprilia, Trialisa Ari Sr Windyaswari Ari Sri Widyaswari Ari Sri Windyaswari Ari Sri Windyaswari Ari Sri Windyaswari, Ari Sri Ayu, Inna Puspa Chandani Nurul Hafizah Choirunnisa, Dinda dessy ratnasari Dewi, Renti Mutiara Dhimas Ariya Wibiksana Dwi Ayu Pratiwi Dwigantina, Ami Soega Dyah Nur Azizah, Dyah Nur Elfahmi Elfahmi Elfahmi Elfahmi, Elfahmi Ema Mutya Endang Kumolowati Esanda Zulfi Amedea Euis Reni Yuslianti Faizal Hermanto Farhan Fikri Alatas Fitriani, Adinda Fitrichia, Anggita Esa Putri Iis Inayati Rakhmat Ismail, Nursafira Khairunnisa Jaka Permana Jovie Mien Dumanauw Julia Ratnawati Julia Ratnawati Khairunisa Harizqi Nurul Husna Limbong, Remare Halomoan Melani, Putri Dwi Muhamad Raihan Maulana Muhammad Irwan Mutya, Ema Niken Tunjung Murti Pratiwi Nira Purnamasari, Nira Nur Achsan Al-Hakim Nur Fauziyyah, Salma Alifia Nurhalizah, Dinda Siti Nurhayati, Viola Aditya Nursafira Khairunnisa Ismail Oktiyas Muzaky Luthfi Oktovia El Shara Paramita, Veronika Santi Pertiwi, Ananda Tunjung Pratiwi, Dwi Ayu Purnama, Uwan Purwoko, Agus Putri, Dhiffa Namira Alifia Ramelan, Riska Sigit Remare Halomoan Limbong Renti Mutiara Dewi Reyhan Adriana Deris Ridwan Ilyas Ridzka Magfirah Rifaz Muhammad Sukma Riska Sigit Ramelan Rizka Khoirunnisa Guntina Rizka Khoirunnisa Guntina RIZKA KHOIRUNNISA GUNTINA, RIZKA KHOIRUNNISA Septiani, Usi Sigid Pamungkas Wicaksono Silvy Julian Siti Muslichah Soraya Riyanti Soraya Riyanti Soraya Riyanti, Soraya Sufyan Zainul Arifin Sugiyanto Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno Sultan Salahudin Jamal SURYANI Suwidjiyo Pramono Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Totik Sri Mariani Tresa Tri Rayani Tresa Tri Rayani, Tresa Tri Trialisa Aprilia Tzazkia Febriyana Akbar Usi Septiani Viola Aditya Nurhayati Weni Widy Astuti Winda Nur Halimah Wulandari, Yesi Yeni Karlina Yeni Karlina Yeni Karlina, Yeni Yenni Karlina, Yenni Yesi Desmiaty, Yesi Yesi Wulandari Yuslianti, Euis Reni Zaini Alfahmi