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Eva Agustina, Eva
Fakultas Peternakan-Perikanan, Universitas Muhammadiyah Malang
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Formulation and Characterization Tests of SCOBY Kombucha Gel Agustina, Eva; Nafisah, Raihana Frika; Fiddaroini, Achmad Farchan; Tania, Clarista Eka; Purnamasari, Risa; Andiarna, Funsu; Hidayati, Irul
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.143-151

Abstract

The wound is the damage to body tissues caused by various factors such as accidents, sharp object scratches, etc. Proper care is necessary to accelerate the wound healing process. Topical medications, such as gel preparations, are commonly used for wounds. Kombucha SCOBY is believed to contain bioactive compounds that can accelerate the wound healing process, making it a potential active ingredient in gel preparations. This research aims to determine the characteristics of kombucha SCOBY gel with various concentration variations. SCOBY is obtained from kombucha fermentation using green tea, sugar, and kombucha culture. Preparing kombucha SCOBY gel involves using carbomer 940, methyl paraben, triethanolamine, glycerin, and SCOBY kombucha. The concentration variations of kombucha SCOBY gel include 0%, 2%, 4%, 8%, 10%, and 12%. Each formula undergoes physical characteristic tests including an organoleptic test, hedonic test, homogeneity test, pH test, spreadability test, and adhesiveness test. Data obtained after physical characteristic tests of the gel preparations are analyzed descriptively. Based on the conducted research, it was found that kombucha SCOBY gel preparations with concentration variations of 0%, 2%, 4%, 8%, 10%, and 12% exhibited different characteristics. The higher the gel concentration, the more acidic the gel's aroma, the more liquid the texture, and the more intense the color. SCOBY added to the gel preparation can increase the pH value, adhesiveness, and spreadability of the gel.
Effect of Different Concentration of SCOBY Kombucha as an Anti-acne Against Staphylococcus aureus in The Ear of Mice (Mus musculus) Ferdyanti, Anggun Putri; Agustina, Eva; Faizah, Hanik; Purnamasari, Risa
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1143-1150

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) results from symbiosis between yeast and bacteria during fermentation. SCOBY kombucha gel combined with orange peel extract has antibacterial compounds that can inhibit the growth of S. aureus bacteria. The method in this study was to make variations in the concentration of SCOBY in the gel and inject S. aureus bacteria into the ears of mice. After 2x24 hours, the diameter of the inflammation caused was observed and SCOBY kombucha gel was applied (0%, 2%, 4%, 8%, 10%, and 12%), positive control (Mediclin 1%), and negative control (without gel application). The results showed that the higher the concentration of SCOBY, the faster the healing activity of the gel against S. aureus. SCOBY kombucha gel has the fastest healing activity in inhibiting acne pathogen S. aureus 12% SCOBY gel. Inflammation in the ears of mice treated with 12% SCOBY gel had healed by the 90th hour, while in other groups of mice it had only healed at the last observation hour, namely the 114th hour.
Co-Authors Abdul Rokhim Adelia Wulandari Agustin, Azlinda Mitha Anastasya, Atira Salma Arini Nurul Mahmudah Arsyntha Permatasari, Rizka Auvaria, Shinfi Wazna Burhana, Nanda Azizah Butarbutar, Marisi Christiana, Indah Darwin Lie Dedy Suprayogi Dwi Ariyani, Anita Esti Tyastirin Fadhiel, Muhammad Ihsan Faidah, Nurul Ilmi Faizah, Hanik Fantastic, Chevy Farida Yulianti Febryanti, Eka Ferdyanti, Anggun Putri Ferri, M. Fiddaroini, Achmad Farchan Firanita, Setiya Ririn Funsu Andiarna Gani Jawak Haqiqiansyah , Gusti Hasimi, Muhammad Hasbi Hidayatin, Nur Irawanto, Roni Irfan Hadi Irul Hidayati Juliani Kholifah, Rifa Ayu Nur Kusumawati, Diana Laminem, Laminem Linda Prasetyaning Widayanti Ma'arif, Muhammad Maria Susanti, Eka Meylani Yopi, Rezita Misbakhul Munir Miskiah, Nur Yohaniz Moch. Irfan Hadi Muammar Khaddafi Muhibbuddin Abdillah, Muhibbuddin Nabila, Salsa Nabila, Syafa Putri Nadia Nadia Nadlir Nadlir, Nadlir Nafisah, Raihana Frika Nana Triapnita Nainggolan Nilandita, Widya Nova Lusiana NR Khoirun Nazazillah, NR Khoirun Pratama, Hendi Yoga Rini Setiyowati Risa Purnamasari Rony Irawanto Rosyada, Fanny Fajrin Aulia Sanabila, Annisa Inda Situmorang, Rostiar Sulistiya Nengse Surfiyanti, Oktarina Tania, Clarista Eka Teguh Taruna Utama Vida Nofrianinda, Vida