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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Dasar AL KAUNIYAH BERKALA SAINSTEK REKAYASA UNEJ e-Proceeding REINWARDTIA Jurnal Biodjati BIOEDUKASI Jurnal Malikussaleh Mengabdi Life Science and Biotechnology Biosaintifika: Journal of Biology & Biology Education Reinwardtia Jurnal Penelitian Sains dan Teknologi Indonesia
Fuad Bahrul Ulum
Jurusan Biologi, FMIPA, Universitas Jember, Jl. Kalimantan No.37, Jember, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Evaluasi Lokus Potensial matK dan ITS2 Untuk DNA Barcoding Anggrek Bulbophyllum lobbii Lindl. Su'udi, Mukhamad; Ulum, Fuad Bahrul; Ardiyansah, Muhammad; Fitri, Nurfajri Eka
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.33897

Abstract

AbstrakBulbophyllum lobbii Lindl. merupakan anggrek dari famili Orchidaceae yang berpotensi sebagai bahan baku obat herbal. Identikasi morfologi anggrek B. lobii memiliki keterbatasan karena kemiripan spesies dengan anggrek lain. Alternatif identifikasi secara molekuler menggunakan sekuen matK dan ITS2 sebagai barcode dalam DNA barcoding diharapkan menjadi salah satu lokus pembeda spesies anggrek B. lobbii secara akurat dan efisien. Penelitian ini bertujuan untuk mengidentifikasi sekuen matK dan ITS2 sebagai penanda molekuler yang efektif untuk anggrek B. lobbii. DNA genom B. lobbii diisolasi dengan metode Cethyl Trimethyl Ammonium Bromide (CTAB) dan amplifikasi DNA dengan PCR. Hasil penelitian menunjukkan sekuen matK dari B. lobbii memiliki tingkat homologi tinggi dengan dua spesies B. lobbii (KY966747.1 dan KY966691.1) dari China dengan nilai Per. Ident sebesar 99,20%, sedangkan sekuen ITS2 memiliki homologi tertinggi dengan nilai Per. Ident sebesar 99,76% pada spesies B. lobbii (MG253848.1) dari Polandia. Hasil analisis menunjukkan sekuen ITS2 dapat mengidentifikasi spesies dari tingkatan subspesies atau diatasnya (ordo atau genus) dan juga meningkatkan resolusi filogenetik yang baik pada hasil BLAST, sedangkan sekuen matK memberikan sedikit kontribusi dalam pengelompokkan hubungan kekerabatan antara spesies B. lobbii dengan spesies pembanding lainnya. Sekuen ITS2 dapat direkomendasikan sebagai penanda molekuler yang paling baik untuk identifikasi sampel anggrek Bulbophyllum, khususnya Bulbophyllum lobbii.AbstractBulbophyllum lobbii Lindl. is an orchid from the Orchidaceae family which has potential as a raw material for herbal medicine. Morphological identification of the B. lobii orchid has limitations due to the species' similarity to other orchids. The alternative molecular identification using matK and ITS2 sequences as barcodes in DNA barcoding is expected to be one of the loci for distinguishing the B. lobbii orchid species accurately and efficiently. This study aims to identify matK and ITS2 sequences as effective molecular markers for the orchid B. lobbii. Bulbophyllum lobbii genomic DNA was isolated using the Cethyl Trimethyl Ammonium Bromide (CTAB) method and DNA amplification by PCR. The results showed that the matK sequence from B. lobbii has a high level of homology with two B. lobbii species (KY966747.1 and KY966691.1) from China with a value of Per. Ident is 99.20%, while the ITS2 sequence has the highest homology with a Per. Ident value of 99.76% with the species B. lobbii (MG253848.1) from Poland. The results of the analysis show that the ITS2 sequence can identify species from the subspecies level or above (ordo or genus) and also improves good phylogenetic resolution in BLAST results, while the matK sequence makes little contribution in grouping the relationship between the B. lobbii species and other comparison species. The ITS2 sequence can be recommended as the best molecular marker for identifying Bulbophyllum orchid samples, especially Bulbophyllum lobbii. 
Community Structure of Avifauna in The Rehabilitation Zone at Wonoasri Resort, Meru Betiri National Park Siddiq, Arif Mohammad; Sulistiyowati, Hari; Ulaa, Munaa Aqidatul; Ulum, Fuad Bahrul; Fadri, Firda
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30033

Abstract

The rehabilitation zone of Meru Betiri National Park (MBNP) needs to be measured in relation to the success of ecosystem restoration. It can be used as a bioindicator, such as the bird community structure, hence their sensitivity to environmental changes. Therefore, the aims of this study are to determine the community structure of avifauna in the rehabilitation zone at Wonoasri Resort, MBNP including bird diversity, richness, dominance, feeding guilds, and their correlation with forest cover. The research was conducted in January 2023 in the Bonangan Block at three observation points, namely low vegetation cover, medium vegetation cover, and high vegetation cover. The ecological data, such as species and abundance, were collected using the point count method, while additional information related to conservation statuses were collected by web browsing on the IUCN Red List, the CITES appendix, and Indonesian government regulation (LHK No.106/2018). Data analysis used the dominance index, species diversity index (Shannon Wiener), and evaluated for statistical data using a Kruskal-Wallis (KW) test in R version 3.2.1. We recorded 38 bird species belonging to 31 genera and 21 families during this study. Among the feeding guilds, the insectivorous birds (14 species) were recorded as the higher species richness, followed by omnivorous (eight species), frugivorous (five species), granivorous (five species), carnivorous (four species), and nectarivorous (two species). The Kruskal-Wallis test shows there is a significant correlation among the feeding guilds (value of 11.644, with p-value = 0.040). Furthermore, referring to species richness, high vegetation cover areas have the highest species richness (30 species) compared to medium vegetation cover areas (27 species) and low vegetation cover areas (26 species). The dominance of avifauna species at this location falls into the low category (C = 0.07). According to the diversity index, avifauna in Bonangan Block is classified as a high category (H'=3.01).
Diversity of Hoya Species across Three Plantation Landscapes in Jember Regency and the Phytochemical and Antioxidant Potential of Hoya lacunosa Blume Ulum, Fuad bahrul; Hayyinah, Asa Hanifatul; Sulistiyowati, Hari; Ratnasari, Tri; Su'udi, Mukhamad; Dwinianti, Edia Fitri; Setyati, Dwi
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.22001

Abstract

Jember Regency encompasses several plantation areas characterized by diverse ecological conditions, notably the Gumitir, Garahan, and Tancak plantations. Among the flora in these regions, the genus Hoya is notable for its medicinal potential. This study aimed to characterize the diversity of Hoya species, provide detailed morphological documentation, and evaluate the phytochemical and antioxidant properties of a medicinally used species. Field exploration employed a systematic plot-based design consisting of 150 plots (5 × 5 m²) with 3 m spacing. The most vegetatively dominant species, Hoya lacunosa, was subjected to GC–MS phytochemical analysis and DPPH-based antioxidant evaluation. A total of eleven Hoya species were recorded across the three plantation sites. The Gumitir plantation exhibited the highest diversity, containing ten species., i.e. Hoya burtoniae, H. camphorifolia, H. carnosa, H. cinnamomifolia, H. dennisii, H. fuscomarginata, H. lacunosa, H. micrantha, H. purpureofusca, and two unidentified Hoya spp. In contrast, the Garahan plantation supported only H. dennisii and H. fuscomarginata, while H. lacunosa was exclusively found in Tancak indicating a highly localized distribution. GC-MS profiling on H. lacunosa leaves revealed 26 compounds, comprising 16 secondary metabolites (12 phenolics, 2 alkaloids, and 2 terpenes) and 5 primary metabolites. The methanol extract exhibited strong antioxidant activity (IC₅₀ = 50.89 ppm), reflecting its potential as a natural source of bioactive compounds. These findings demonstrate substantial Hoya diversity in Jember Regency and underscore the potential of H. lacunosa as a valuable source of bioactive metabolites for pharmaceutical development.
Co-Authors Afiatri Putrika AFIATRI PUTRIKA AFIATRI PUTRIKA, AFIATRI Afidah, Yusfi Angraeni, Devi Silvia Ardiyansah, Muhammad Arif Mohammad Siddiq Arsetyo Rahardianto ‘Ain, Salsabila Qurrotu DIAN APRIANI DIAN APRIANI DWI INDAHNING ROHMAH Dwi Setyati Dwinianti, Edia Fitri Eny Yuniati Fadri, Firda FADZILAH AG FADZILAH AG. KANAK FADZILAH AG. KANAK, FADZILAH AG. Farhan, Abdillah Maulana Fitri, Edia Fitri, Nurfajri Eka Gilani, Noor Azizah Hayyinah, Asa Hanifatul Hidayat Teguh Wiyono Hidayatullah, Alhabsy Indah Wahyuni INDAH WAHYUNI KANAK KANJANA WONGKUNA KANJANA WONGKUNA KANJANA WONGKUNA, KANJANA Khoirunnisa, Gita Ayu Kurniawan, Ludfi Laili, Alvina Nur LESLEY C. LUBOS LESLEY C. LUBOS LESLEY C. LUBOS, LESLEY C. LUONG THIEN TAM LUONG THIEN TAM LUONG THIEN TAM, LUONG THIEN Miatin Alvin Septianasari Mika Rizki Puspaningrum MIKA RIZKI PUSPANINGRUM MIKA RIZKI PUSPANINGRUM, MIKA RIZKI MOHD RAWIYANI PG. HJ. SERUDIN MOHD RAWIYANI PG. HJ. SERUDIN MOHD RAWIYANI PG. HJ. SERUDIN, MOHD RAWIYANI PG. MONICA SULEIMAN MONICA SULEIMAN MONICA SULEIMAN, MONICA Mudinillah, Adam Mukhamad Su'udi Musyarofah Zuhri MUSYAROFAH ZUHRI Musyarofah Zuhri NG AIK MIN NG AIK MIN NG AIK MIN, NG AIK NURLISMA JUNITA NURLISMA JUNITA NURLISMA JUNITA, NURLISMA Nursahara Pasaribu NURSAHARA PASARIBU Nursahara Pasaribu Ratnasari, Tri Rendy Setiawan Rizqoni, Moh Ikmal Alfi ROBBERT GRADSTEIN ROBBERT GRADSTEIN ROBBERT GRADSTEIN, ROBBERT Rosida, Waki'atil Rosida, Wakiatil SATRIYAS ILYAS SOONTHREE KORNOCHALERT SOONTHREE KORNOCHALERT SOONTHREE KORNOCHALERT, SOONTHREE Su'udi, M. Su'udi, Mukhamad Sudarmadji, S. Sulistiyowati, Hari Su’udi, Mukhamad Ulaa, Munaa Aqidatul Veren Yuliana Saputri Wulandari, Dyah Retno YONG KIEN–THAI, YONG YONG KIEN–THAI YONG KIEN–THAI