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RESPONS PERTUMBUHAN MISELIA Coprinus comatus AKIBAT PEMBERIAN BEBERAPA JENIS AN KONSENTRASI EKSTRAK TUMBUHAN SECARA IN VITRO Amelia, Rizki Nurfitrah; Lestari, Ani; Adhi, Satriyo Restu
Jurnal Ilmiah Hijau Cendekia Vol 11 No 1 (2026): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v11i1.5616

Abstract

Coprinus comatus merupakan jamur yang merugikan dalam budidaya jamur merang karena memiliki kemampuan menghambat pertumbuhan jamur merang. Coprinus comatus bersifat kompetitif sehingga menyebabkan penurunan pasokan nutrisi yang optimal untuk pertumbuhan jamur merang. Tujuan penelitian ini untuk mendapatkan ekstrak tanaman yang dapat menekan pertumbuhan miselia jamur kompetitor Coprinus comatus secara in vitro. Penelitian ini dilaksanakan di Laboratorium Bioteknologi dan Pemuliaan Tanaman Fakultas Pertanian Universitas Singaperbangsa Karawang dimulai dari Desember 2023 sampai Februari 2024. Dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) Faktor tunggal terdiri dari 10 perlakuan yaitu, A (PDA 100%), B (PDA + EDP 2%), C (PDA + EDP 4%), D (PDA + EDP 6%), E (PDA + EDP 8%), F (PDA + ESW 2%), G (PDA + ESW 4%), H (PDA + ESW 6%), I (PDA + ESW 8%), J (PDA + Karbendazim) diulang sebanyak 3 kali. Data dianalisis menggunakan analisis ragam dan apabila uji F Taraf 5% signifikan, maka untuk mengetahui perlakuan yang terbaik dilakukan dengan uji lanjut Duncan Multiple Range Test (DMRT) taraf 5%. Hasil penelitian menunjukkan bahwa ekstrak daun pepaya 8% memberikan respons penghambatan terbaik pada laju pertumbuhan miselia sebesar 0,94 cm/hari dan penambahan bobot miselia terkecil 0,70 g/hari.
Uji Aplikasi Kitosan Sebagai Edible Coating dalam Pengendalian Penyakit Antraknosa (Colletotrichum spp.) Pada Buah Pisang Cavendish (Musa acuminata L.) di Laboratorium Erica Margaretha; Satriyo Restu Adhi
JURNAL PERTANIAN Vol 17, No 1 (2026): Jurnal Ilmiah Respati
Publisher : Universitas Respati Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/jir.v17i1.6240

Abstract

Anthracnose (Colletotrichum spp.) is a major postharvest disease that reduces the quality of Cavendish banana (Musa acuminatata L.) fruit. This study aims to evaluate the effect of chitosan application as an edible coating material on anthracnose disease of banana fruit, with a focus on symptom diameter and weight loss. The research was conducted in vitro at the Laboratory of Plant Disturbing Organisms, Faculty of Agriculture, Singaperbangsa University of Karawang, in November-December 2024, using a completely randomized design with five concentrations of 0%, 0.5%, 1%, 2%, and 3% chitosan, with five replications. The results showed that the application of 2% chitosan effectively suppressed the development of anthracnose disease symptoms, with an average symptom diameter of 2.77 cm and produced a percentage reduction in weight loss of 10.42%. Meanwhile, 3% chitosan concentration produced the best percentage of weight loss suppression, which was only 7.07%. In contrast, the control treatment showed the highest severity with a symptom diameter of 5.13 cm and a percentage of weight loss of 14.61%. These findings indicate that chitosan, especially at 2% concentration, is effective as an anthracnose control agent on Cavendish banana during storage
Uji Formulasi Bahan Pembawa Jamur Antagonis Gliocladium sp. Terhadap Penyakit Layu Fusarium (Fusarium oxysporum f.sp. cepae) Tanaman Bawang Merah (Allium ascalonicum L.) Siska Luthfiah; Satriyo Restu Adhi; Lutfi Afifah
JURNAL PERTANIAN Vol 17, No 1 (2026): Jurnal Ilmiah Respati
Publisher : Universitas Respati Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/jir.v17i1.6439

Abstract

Fusarium wilt disease caused by the fungus Fusarium oxysporum is one of the main diseases affecting shallots and can cause losses of up to 50%. Gliocladium sp. has the potential to be used as a biological agent because it is able to control pathogens and suppress the development of fusarium wilt disease. This study aims to determine which carrier material is most effective in producing the highest spore density of Gliocladium sp. and its antagonistic ability in reducing the intensity of Fusarium wilt disease in red onions. The study was conducted experimentally using a completely randomised single-factor design to test the spore density of Gliocladium sp. on four types of carrier materials: U₁ (rice bran), U₂ (rice), U₃ (corn cobs), and U₄ (sorghum seeds) with six replications, and a single-factor Randomised Block Design to test Gliocladium sp. against Fusarium oxysporum wilt disease with seven treatments: G₀ (control), G₁ (fungicide), G₂ (Gliocladium sp. from rice bran), G₃ (Gliocladium sp. from rice), G₄ (Gliocladium sp. from corn kernels), G₅ (Gliocladium sp. from sorghum seeds), G₆ (commercial Gliocladium sp.), and four replications. The results of the study indicate that the use of various carrier materials significantly affects the spore density of Gliocladium sp. and in suppressing Fusarium wilt disease in red onions (Fusarium oxysporum f.sp. cepae). Treatment U₂ (rice) produced the highest spore density, 2.70 x1011 spores/ml. However, treatment G₃ (Gliocladium sp. on rice) yielded suboptimal results in terms of germination rate (20.0%) and disease severity (100%), thus failing to effectively suppress Fusarium wilt disease.
Pertumbuhan Trichoderma sp. pada Beberapa Bahan Pembawa dan Kemampuannya Mengendalikan Penyakit Hawar Pelepah Padi Sofi Kamilah Rahmatika; Satriyo Restu Adhi; Tatang Surjana
JURNAL PERTANIAN Vol 17, No 1 (2026): Jurnal Ilmiah Respati
Publisher : Universitas Respati Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/jir.v17i1.6248

Abstract

Sheath blight (Rhizoctonia solani) is an important disease of rice plants that can cause yield losses of up to 20% and will continue to increase if the severity is high. Excessive use of fungicides has a negative impact on living things and the environment. The solution that can be found is to utilize biological agents such as Trichoderma sp. to control plant diseases. The purpose of this study was to determine the effect of using several carrier materials on the growth of Trichoderma sp. and its ability to suppress rice sheath blight. This study used an experimental method with a Completely Randomized Design (CRD) Single Factor for the growth test of Trichoderma sp. on several carrier materials consisting of 4 treatments (B1, B2, B3, B4) and 6 replications, as well as a Single Factor Randomized Block Design (RBD) for the test of the ability of Trichoderma sp. to suppress sheath blight with 7 treatments (F0, F1, F2, F3, F4, F5, F6) and 4 replications. The results of the study showed that Treatment B4 (Parboiled Rice + Dry Chicken Manure + Sugar) produced the best growth of Trichoderma sp., and each Trichoderma sp. treatment had no significant effect on seed germination and the incidence of leaf sheath blight disease
Kitosan Sebagai Bahan Edible Coating Buah Stroberi (Fragaria x ananassa) Terhadap Jamur Rhizopus Stolonifer Penyebab Penyakit Busuk Lunak Anita Dewi; Satriyo Restu Adhi; Fauzia Mustikasari
JURNAL PERTANIAN Vol 16, No 3 (2025): Jurnal Ilmiah Respati
Publisher : Universitas Respati Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/jir.v16i3.6429

Abstract

The soft rot disease caused by the pathogen Rhizopus stolonifer is one of diseases that causes significant losses during the storage and distribution of post-harvest fruit. The purpose of this study is to determine the effectiveness of using chitosan and its various concentrations in reducing soft rot disease in strawberries. The study was conducted in vitro at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Singaperbangsa Karawang, from February to March 2025. The experiment use randomized complete block design with five treatments: control (K0), chitosan 0.5% (K1), chitosan 1% (K2), chitosan 1.5% (K3), fungicide (F) as the positive control, and each treatment was repeated five times with four samples each. The results showed that the 1.5% chitosan treatment (K3) had a significant impact on the disease severity treatments, and significantly affected the weight loss parameter, with the lowest weight loss value compared to other treatments. These findings suggest that chitosan, particularly at the consentration of 1.5%, is effective in controlling soft rot disease in strawberries during storage.