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All Journal Journal of Tropical Biodiversity and Biotechnology BIOEDUSCIENCE Jurnal Biologi Tropis Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jambura Edu Biosfer Journal Agroteknika Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Hydrogen: Jurnal Kependidikan Kimia Bioscientist : Jurnal Ilmiah Biologi BIOVALENTIA: Biological Research Journal Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Journal of Applied Community Engagement (JACE) Filogeni: Jurnal Mahasiswa Biologi Journal of Tropical Mycorrhiza Jurnal Abdimas Terapan Biodik: Jurnal Ilmiah Pendidikan Biologi Science: Indonesian Journal of Science Damhil: Jurnal Pengabdian kepada Masyarakat Bioma : Jurnal Biologi dan Pembelajaran Biologi Inventa: Journal of Science, Technology, and Innovation
Devi Bunga Pagalla
Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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In vitro germination of lemon cui (Citrus microcarpa Bunge) Pagalla, Devi Bunga; Kandowangko, Novri Youla; Ahmad, Jusna; Febriyanti, Febriyanti
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3952

Abstract

Lemon cui (Citrus microcarpa Bunge) is widely utilized by the people of Gorontalo as a cooking ingredient, traditional medicine, cleaning agent, and cosmetic ingredient. This diversity of uses indicates the potential for further development of lemon cui. The initial stage of development that can be undertaken is the mass propagation of high-quality lemon cui seedlings. This study aims to produce lemon cui seedlings using in vitro culture techniques through seed germination.The experimental design used in this study is a Completely Randomized Design (CRD) with four treatments, namely:  A: MS0 (without the addition of plant growth regulators and young coconut water); B: MS + 50 mL young coconut water + 0.5 ppm 2,4-D; C: MS + 50 mL young coconut water + 1 ppm 2,4-D; and D: MS + 50 mL young coconut water + 1.5 ppm 2,4-D. The culture medium used was Murashige and Skoog (MS) supplemented with young coconut water and 2,4-dichlorophenoxyacetic acid (2,4-D). The study's results showed that using young coconut water and 2,4-D significantly accelerated the germination of lemon cui seeds. The best result was observed in treatment D, where the citrus seeds exhibited a maximum germination rate of 100%. In addition, the combination of young coconut water and 2,4-D is capable of inducing callus formation during the germination process. The combination of 50 mL of young coconut water and 1.5 ppm 2,4-D is the most effective concentration for inducing germination of lemon cui seeds and callus formation.
Pengaruh Model Pembelajaran Project Based Learning Terhadap Keterampilan Kolaborasi Dan Kreativitas Siswa Pada Materi Struktur Dan Fungsi Jaringan Tumbuhan Di Kelas Xi Sma Negeri 1 Kabila: (The Influence of the Project Basic Learning Model on Students' Collaboration and Creativity Skills on the Material of Plant Tissue Structure and Function in Class XI of SMA Negeri 1 Kabila) Menanti, Menanti; Ahmad, Jusna; Usman, Nurul Fajryani; Dama, Lilan; Febriyanti, Febriyanti; Pagalla, Devi Bunga
BIODIK Vol. 11 No. 02 (2025): June 2025
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biodik.v11i02.44609

Abstract

The purpose of this research is to determine the effect of the Project-Based Learning model on Collaboration and Creativity Skills in XI grade students of SMA Negeri 1 Kabila. This research is an experimental quantitative study with a (Nonequivalent Control Group Design). The sample in this study consisted of 20 students. The data analysis technique was complemented by statistical data processing methods. The average pretest score for Collaboration Skills in the experimental class was 37.60, while in the control class it was 35.20. The average pretest score for Creativity Skills in the experimental class was 30.20, and in the control class, it was 28.20. Meanwhile, the average posttest score for Collaboration Skills in the experimental class was 44.25, and in the control class, it was 37.20. The average posttest score for Creativity Skills in the experimental class was 37.05, and in the control class, it was 29.00. These results indicate that there is a significant effect before and after the treatment. Based on the t-test results with a t-table value of 2.086, the influence value for collaboration skills is 2.548 and the influence value for creativity skills is 14.209. In other words, t calculated > t table, thus H0 is rejected and H1 is accepted. There is an effect of the Project Based Learning Model on Collaboration and Creativity Skills of Students in the Material of Structure and Function of Plant Tissues in Class XI of SMA Negeri 1 Kabila. Abstrak. Tujuan penelitian ini adalah untuk mengetahui pengaruh model Project Based Learning terhadap Keterampilan Kolaborasi dan Kreativitas pada Siswa Kelas XI SMA Negeri 1 Kabila. Penelitian ini merupakan penelitian eksperimen kuantitatif dengan jenis penelitian (Nonequivalent Control Group Design). Sampel dalam penelitian ini sebanyak 20 siswa. Teknik analisis data dilengkapi dengan metode pengolahan data statistik. Rata-rata pretest Keterampilan Kolaborasi kelas eksperimen sebesar 37,60 dan kelas kontrol sebesar 35,20. Rata-rata pretest Keterampilan Kreativitas kelas eksperimen sebesar 30,20 dan kelas kontrol sebesar 28,20. Sedangkan rata-rata posttest Keterampilan Kolaborasi kelas eksperimen sebesar 44,25 dan kelas kontrol sebesar 37,20. Sedangkan rata-rata posttest Keterampilan Kreativitas kelas eksperimen sebesar 37,05 dan kelas kontrol sebesar 29,00. Hasil tersebut menyatakan bahwa terdapat pengaruh yang signifikan sebelum dan sesudah perlakuan. Berdasarkan hasil uji-t dengan t tabel yaitu sebesar 2,086, diperoleh nilai pengaruh untuk keterampilan kolaborasi sebesar 2,548 dan nilai pengaruh untuk keterampilan kreativitas sebesar 14,209. Dengan kata lain t hitung > t tabel, maka H0 ditolak dan H1 diterima. Terdapat Pengaruh Model Project Based Learning terhadap Keterampilan Kolaborasi dan Kreativitas Siswa pada Materi Struktur dan Fungsi Jaringan Tumbuhan Kelas XI SMA Negeri 1 Kabila.
Pengembangan Modul Keanekaragaman Genetik Jagung Lokal Gorontalo Untuk Pembelajaran Biologi SMA: (Development of a Genetic Diversity Module for Gorontalo Local Corn for High School Biology Learning) Hidayat, Ahmad; Kandowangko, Novri Youla; Latjompoh, Masra; Katili, Abubakar Sidik; Pagalla, Devi Bunga
BIODIK Vol. 11 No. 02 (2025): June 2025
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biodik.v11i02.44614

Abstract

The lack of contextual teaching materials that highlight local potential is a challenge in biology learning in schools. This study aims to determine the validity and practicality of the learning module on genetic biodiversity in local Gorontalo corn plants. This study uses the Research and Development method. The learning module was developed using the ADDIE model, up to the limited trial stage. The research subjects were 25 grade X high school students. The learning module was validated by material experts and media experts, and the practicality of using the module in learning biodiversity material was tested. The validation results showed that the module was very valid with an average percentage of 98% from material experts and 93.77% from media experts. The practicality of the module was also very high based on teacher responses of 97.77% and student responses of 98.06%. From the results of the validity and practicality tests, it can be concluded that the learning module on genetic biodiversity in local corn plants can be used in learning Biology for grade X high school, biodiversity material in schools. Abstrak. Kurangnya bahan ajar kontekstual yang mengangkat potensi lokal menjadi tantangan dalam pembelajaran biologi di sekolah. Penelitian ini bertujuan untuk mengetahui validitas dan kepraktisan modul pembelajaran keanekaragaman hayati tingkat genetik pada tanaman jagung lokal Gorontalo. Penelitian ini menggunakan metode Research and Development.  Modul pembelajaran dikembangkan dengan menggunakan model ADDIE, hingga tahapan uji coba terbatas. Subjek penelitian adalah 25 peserta didik kelas X SMA. Modul pembelajaran divalidasi oleh ahli materi dan ahli media, serta diuji kepraktisan penggunaan modul dalam pembelajaran materi keanekaragaman hayati.  Hasil validasi menunjukkan bahwa modul sangat valid dengan rata-rata persentase 98% dari ahli materi dan 93,77% dari ahli media. Kepraktisan modul juga sangat tinggi berdasarkan respons guru sebesar 97,77% dan respons peserta didik sebesar 98,06%. Dari hasil uji validitas dan kepraktisan, maka dapat disimpulkan bahwa modul pembelajaran keanekaragaman hayati tingkat genetik pada tanaman jagung lokal dapat digunakan dalam pembelajaran Biologi kelas X SMA, materi  keanekaragaman hayati  di sekolah.
A In Vitro Callus Induction from (Antidesma bunius L.) Leaves Adju, Fitria Y.Hs.; Ahmad, Jusna; pagalla, Devi Bunga; Kandowangko, Novri Youla; Husain, Indriati; Isima , Lindawaty
BIOVALENTIA: Biological Research Journal Vol. 11 No. 1 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.1.2025.451

Abstract

Antidesma bunius L. is a tree species that has ecological and economic benefits, and has many bioactivities. Propagation of this tree can be done in several ways, one of which is callus induction. Callus is a collection of undifferentiated cells that can develop into new plants with the help of ZPT. The purpose of this study was to determine the effect and optimum combination of ZPT 2,4-D and BAP on the induction of leaf callus of A. bunius L. The research design was a non-factorial Completely Randomised Design (CRD) with 4 treatments namely P0: MS without the addition of zpt; P1: MS + 1 ppm BAP + 3 ppm 2,4-D; P2: MS + 2 ppm BAP + 2 ppm 2,4-D; P3: MS + 3 ppm BAP + 1 ppm 2,4-D. Parameters observed included callus emergence time, percentage of callus explants, and callus morphology. The results showed that P2 is the best concentration for callus induction, by looking at the observed effect on callus emergence time of 6 HST, percentage of callus explants 56% and callus morphology which is white with crumb texture, which can develop into embryogenic callus
Analisis Tahapan Perkembangan Mikrospora Citrus hystrix Pagalla, Devi Bunga; Ahmad, Jusna; Ramlah, Ramlah
Bioma : Jurnal Biologi dan Pembelajaran Biologi Vol. 10 No. 1 (2025): BIOMA: JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v10i1.2592

Abstract

Setiap mikrospora berpeluang untuk berkembang menjadi individu baru apabila dikulturkan dalam medium yang sesuai karena setiap sel tersebut memiliki sifat totipotensi. Penelitian ini bertujuan untuk menganalisis tahap perkembangan mikrospora berdasarkan panjang kuncup bunga tanaman jeruk purut yang beragam. Tahap ini merupakan langkah awal yang sangat penting sebelum memulai program pemuliaan tanaman menggunakan teknik kultur antera atau mikrospora. Dalam penelitian ini, tahap perkembangan mikrospora diamati pada 13 kuncup bunga yang telah diukur panjangnya. Mikrospora diamati dengan metode sederhana, yaitu menghancurkan dua antera dalam cawan petri berisi lima tetes air steril, kemudian campuran tersebut dipindahkan ke kaca preparat untuk pengamatan mikroskopis. Selain itu, dilakukan juga pengamatan morfologi antera, penghitungan jumlah antera, dan kelopak bunga pada setiap kuncup bunga. Hasil penelitian menunjukkan bahwa di antara 13 kuncup bunga yang diamati, tidak semua tahap perkembangan mikrospora teramati. Tahap serbuk sari matang (mature pollen) lebih sering teramati. Hasil penelitian ini dapat digunakan sebagai acuan untuk mengoptimalisasi proses identifikasi kondisi morfologi kuncup bunga jeruk purut yang mengandung mikrospora untuk memulai kultur mikrospora.
Comparison of Anatomical Structure of Maize (Zea mays L.) Plant of Gorontalo Local Variety Pulut and Momala Ahaya, Cindi; Kandowangko, Novri Youla; Febriyanti, Febriyanti; Ahmad, Jusna; Pagalla, Devi Bunga
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8483

Abstract

One of the primary agricultural commodities that contributes significantly to global food security is maize (Zea mays L.), a monoecious cereal plant of the Poaceae family with distinct but identical male and female blooms. Local cultivars like Pulut and Momala in Gorontalo have exceptional promise that requires further study, particularly with regard to their anatomical makeup. This study Comparing the two types' stem and leaf anatomical features, including vascular tissue (xylem and phloem), epidermis, and stomata, is the goal of this study. A qualitative descriptive approach was used to make the observations, which were subsequently evaluated and shown as a comparison table. The results showed that at the age of 30 and 40 HST, the network of vessels (xylem and phloem) and epidermis on the stems and leaves of Pulut and Momala corn varieties reflected a development pattern that supported the transportation of water, minerals, and nutrients. The number of stomata in Pulut varieties decreased due to less than optimal growth, while Momala varieties showed an increase in the number of stomata which was influenced by the growth period with environmental conditions suitable for corn plants.  
Pemanfaatan Hasil Tangkapan Ikan Dengan Pelatihan Pembuatan Sossis Ikan Di Desa Bilungala Kecamatan Bonepantai Kabupaten Bone Bolango Mustaqimah, Nur; Usman, Nurul Fajryani; Pagalla, Devi Bunga; Nurhayati, Nurhayati
Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Vol 12, No 3 (2023): Jurnal Sibermas (Sinergi Bersama Masyarakat)
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/sibermas.v12i3.17910

Abstract

One of the areas in Gorontalo Province that has fishery potential from Tomini Bay is Bilungala Village, where the most commonly caught fish is Cakalang (Katsuwonus pelamis). Cakalang has a fairly high economic value and is much preferred because it has good meat density, especially to be used as a source of protein. But so far, the community does not have much knowledge about the management of Cakalang, which is the main catch. One of the processed products that can be made with Cakalang is fish sausage. Sausage is a food product that has nutritional value and meets the needs of the body. This processed product is also more practical to serve. The resulting product can also be an alternative business to improve the community's economy. Therefore, a fish processing training activity was carried out in the form of cakalang fish sausage. The aim of this training is to equip housewives in the village with skills in processing fish catches into nutritious and durable food products. The location of this community service activity was carried out in Bilungala Village, Bonepantai District, Bone Bolango Regency. The target is housewives in the village. The activity was carried out on November  which consisted of the preparation stage by conducting a survey and the implementation stage by conducting training with direct demonstrations and together with the participants making the product. This activity went smoothly. Participants were very enthusiastic about participating in the activity from the beginning of the activity to the end of the activity. This activity requires follow-up in the form of marketing training for processed fish products in the form of fish sausage. The training can be an asset for participants consisting of housewives to market their products which in the end are able to increase their income and help the community's economy.
Calcium Assay of Tuna Bone Waste with Atomic Absorption Spectrophotometer (AAS) Najmah, Najmah; Ramadhani, Andi Indra Wulan Sari; Pagalla, Devi Bunga; Arviani, Arviani; Sangkota, Vivi Dia Afrianti
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 1 (2025): February 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i1.14705

Abstract

Yellowfin tuna in Gorontalo is very abundant and is often exported outside the region and even abroad as fillets, so yellowfin tuna bones are also very plentiful. The community frequently processes yellowfin tuna bones into kuah asam or namely ilahe. One of the sources of ilahe is Averrhoa bilimbi L., but the leftover tuna bones are only a source of pollution. This research focuses on processing the waste into a product of high nutritional value and highlights the novelty of a more environmentally friendly fish bone calcium extraction method. One of the main novelties of this research is the use of natural materials, namely star fruit, as an alternative for calcium extraction, which reduces dependence on harmful chemicals and supports the principle of sustainability. The extraction method used heat, followed by drying to reduce moisture content, pulverizing (blender) to reduce particle size and sieving. The calcium content in tuna bone meal was then analyzed using the Atomic Absorption Spectroscopy (SSA) method. The results showed that the tuna bone meal was white in colour, and the calcium content was 184.4890 mg/g or 18.45%. This product has the potential to be applied in various fields, such as food, health, animal feed, pellets, and organic fertilizer, as well as a solution to reduce fishery waste and support environmental sustainability.
Induksi Kalus pada Tangkai Daun dan Batang Muda Tepurang (Momordica Cochinchinensis) dengan Kombinasi Bap dan Iaa Secara In Vitro Fadlia Dunggio; Devi Bunga Pagalla; Jusna Ahmad; Novri Youla Kandowangko; Febriyanti Febriyanti
Science: Indonesian Journal of Science Vol. 1 No. 6 (2025)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/science.v1i6.246

Abstract

Tepurang (Momordica cochinchinensis) memiliki potensi besar dalam bidang farmasi dan pangan, namun keterbatasan bibit dan rendahnya tingkat perkecambahan biji menghambat perbanyakannya. Kultur jaringan menjadi alternatif dalam pelestarian dan perbanyakan tanaman ini, terutama melalui induksi kalus. Penelitian ini bertujuan untuk mengevaluasi pengaruh kombinasi Benzylaminopurine (BAP) dan Indole Acetic Acid (IAA) terhadap induksi kalus dari batang muda M. cochinchinensis secara in vitro. Eksplan berupa batang muda ditanam pada media Murashige & Skoog (MS) dengan kombinasi BAP dan IAA dalam berbagai konsentrasi. Hasil penelitian menunjukkan bahwa perlakuan dengan kombinasi ZPT mampu menginduksi pembentukan kalus dengan warna putih dan tekstur friable setelah 2 minggu subkultur. Temuan ini diharapkan dapat berkontribusi dalam pengembangan metode perbanyakan M. cochinchinensis secara in vitro untuk mendukung konservasi dan pemanfaatannya dalam bidang bioteknologi.
Efektivitas Pupuk Organik Cair Daun Dumbaya (Momordica cochinchinensis) Terhadap Hasil Panen Kedelai Edamame Restiyani Widia Putri; Jusna Ahmad; Devi Bunga Pagalla; Chairunnisah J. Lamangantjo; Hayati Ningsih Gubali
Science: Indonesian Journal of Science Vol. 2 No. 1 (2025)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/science.v2i1.276

Abstract

Dumbaya (Momordica cochinchinensis) merupakan tanaman herbal lokal gorontalo yang berpotensi sebagai bahan baku pupuk organik cair (POC). Penelitian ini bertujuan mengevaluasi efektivitas POC daun Dumbaya terhadap hasil panen kedelai edamame (Glycine max (L.) Merr.). Penelitian menggunakan Rancangan Acak kelompok (RAK) dengan lima perlakuan dan tiga ulangan. Parameter yang diamati meliputi jumlah buah buah, berat buah, dan jumlah polong per tanaman. Hasil menunjukkan bahwa perlakuan R4 (POC 45%) menghasilkan rata-rata jumlah buah 20,92 per pohon, mendekati perlakuan R5 (NPK 2 gr/tanaman) yaitu 22,42 buah. Berat buah tertinggi diperoleh pada R5 (46,7 gr), diikuti R4 (45 gr). Jumlah polong tertinggi juga ditemukan pada R5 (44,41), disusul R4 (41,08). Meskipun hasil menunjukkan tren positif pada POC 45%, uji ANOVA menunjukkan tidak ada perbedaan signifikan antar perlakuan (p>0,05). Secara deskriptif, POC daun Dumbaya menunjukkan potensi sebagai alternatif pupuk ramah lingkungan.
Co-Authors Abubakar Sidik Katili Adelia Salsabila Rahman Adilah Nidaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Aisyah Ahmad Andi Nursanti Ari Indrianto Arviani Arviani Aulia Rahmadani Chairunnisah J Lamangantjo Chairunnisah J Lamangantjo Endang Semiarti Evita Moni Damayanti Fadlia Dunggio Febriyanti Febriyanti febriyanti febriyanti Fitria H.Ys. Adju Haerani Haerani Hartono D Mamu Hartono D. Mamu Hayati Ningsih Gubali Hayatiningsih Gubali Herinda Mardin Indayani Tandililing Indiriati Husain Indriati Husain Isima , Lindawaty Jusna Ahmad Lilan Dama M. Irfan Magfirahtul Jannah Maryani Maryani Masra Latjompoh Masrid Pikoli Menanti, Menanti Miftahul F. Adudu Muhammad Isra Mustaqimah, Nur Najmah Najmah Najmah, Najmah Nazwa S. Lamante Novri Youla Kandowangko Novri Youla Kandowango Nur Mustaqimah Nurhayati Nurhayati Nurhayati Nurhayati Nurman Nurman Nurul Fajryani Usman Nurul Iqraini D Rahmah, Mufti Hatur Ramadhani, Andi Indra Wulan Sari Ramlah Ramlah Ramlah Ramlah Rasydah Nur Tuada Restiyani Widia Putri Retno Sari Rika Eyato Risna A. Onu Sangkota, Vivi Dia Afrianti Sintia S. Ahmad St Hijrah Alfiani Tri Vinesa Moha Walangadi, Farhana Rahmatia Widyawati S. Isima Wirnangsi Din Uno Wirnangsih D. Uno Yuliana Retnowati