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All Journal MPI (Media Pharmaceutica Indonesiana) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan JCPS (Journal of Current Pharmaceutical Sciences) JURNAL FARMASIMED (JFM) Jurnal Pengabdian Al-Ikhlas Jurnal Pengabdian Kepada Masyarakat (Mediteg) Jurnal Jejaring Matematika dan Sains Borneo Journal Of Pharmascientech Jurnal Pengabdian UNDIKMA Jurnal Insan Farmasi Indonesia Jurnal Ilmiah Kesehatan (JIKA) Tropical Health and Medical Research Journal Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal AFAMEDIS Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Bakti Untuk Negeri Benzena Pharmaceutical Scientific Journal Jurnal Pengabdian Masyarakat Panacea Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Anna Khumaira Sari
Universitas Lambung Mangkurat
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Journal : JURNAL FARMASIMED (JFM)

 1 
Antimicrobial Activity of Ethanol Extract of Sea Pandan Leaves (Pandanus odorifer) Against Staphylococcus aureus and Escherichia coli Rizki, Muhammad Ikhwan; Sari, Anna Khumaira; Rahma, Salsabila Fadiya; Rahmatullah, Satrio Wibowo; Izma, Hayatun
Jurnal FARMASIMED (JFM) Vol 7 No 1 (2024): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v7i1.2280

Abstract

Sea pandan (Pandanus odorifer) is used in traditional medicine in India. Sea pandan is efficacious in treating pain, parasites, digestive, liver, and cognitive disorders. Sea pandan is found in coastal areas in South Kalimantan. Sea pandan contains flavonoid compounds that have the potential to be antimicrobials. This study aimed to determine the antimicrobial activity of sea pandan leaf extract. The study began with sample preparation, which included washing the leaves, drying them using an oven, refining the particle size, extracting 70% ethanol, and evaporating the solvent until a thick extract was obtained. Total flavonoid levels in sea pandan leaf extract were determined using the colourimetric method using quercetin standards. Antimicrobial testing was conducted using the diffusion method at concentrations of 30%, 40%, and 50% against Staphylococcus aureus and Escherichia coli. The positive control used was chloramphenicol. The determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values ??was carried out using the dilution method. The results showed that sea pandan leaf extract contains phenolics, flavonoids, saponins, steroids, and tannins. The total flavonoid content in sea pandan leaf extract is 10.01% w/w equivalent of quercetin. The antimicrobial activity of sea pandan leaf extract against Staphylococcus aureus bacteria is 10.15 - 25.36 mm, while against Escherichia coli in the range of 8.15 - 23.16 mm. Ethanol extract of sea pandan leaves at a concentration of 50% is included in the very strong category in inhibiting Staphylococcus aureus and Escherichia coli. Ethanol extract of sea pandan leaves has an MIC value of 7.5% on Staphylococcus aureus and 15% on Escherichia coli. MBC of sea pandan extract is 15% on Escherichia coli bacteria. This study concludes that the ethanol extract of sea pandan leaves has antimicrobial activity against Staphylococcus aureus (bacteriostatic) and Escherichia coli (bactericidal).
Co-Authors Ahmad Ahmad Ainun Mardiah Aisyah, Noor Akhsanul Rahmatullah Alfandani, Gina Amalia Khairunnisa Amalia Khairunnisa, Amalia Amalia Khirunnisa Amilia, Jariyah Anisa Desriyanti Aryzki, Saftia Atikah Andria Saputri Ayu Soraya Ayuchecaria, Noverda Azhara, Desiya Damayanti Buih, Putri Helena Junjung Desriyanti, Anisa Devi Eka Pratama Devia Pratiwi Dewi Kartika Dewi, Anita Ratna Difa Intannia Eka Kumalasari Elisya Fatmawati Erna Prihandiwati Erna Prihandiwati Fadlilaturrahmah Fadlilaturrahmah, Fadlilaturrahmah Fadlillaturahmah Fadlillaturahmah Febrianti, Dwi Rizki Gina Alfandani Herningtyas Nautika Lingga Isnaini, Nazhifah Izma, Hayatun Jariyah Amilia Khairunnisa Khairunnisa Khairunnisa Khairunnisa Khoerul Anwar Khoirunnisa Muslimawati Liling Triyasmono Mahdjati Fitria Mawaddah Mawaddah Meilisa Eka Pratiwi Mia Fitriana Mochammad Maulidie Alfiannor Saputera muhammad fikri Muhammad Ikhwan Rizki Muhammad Itqan Mazdadi Muhammad Rusydi Taufik Nazhipah Isnani Noor Cahaya Normaidah, Normaidah Normila Hayati Novia Ariani Nurul huda Prasdianto Prasdianto Rahma, Salsabila Fadiya Rahmah, Indah Noor Rahmah, Maslia RAHMANIAH RAHMANIAH Rahmatullah, Satrio Wibowo Rahmawati, Nanda Hesti Rahmi Rahmi Rakhmadhan Niah Ramadhani, Muhammad Irfan Renny Renny Risma Ayati Riza Alfian Saftia Aryzki Saftia Aryzki Sandi, Dita Ayulia Dwi Saputra, Adryan Maulana Saputri, Atikah Andria Sari, Fitri Novita Shela Puzi Dina Silviana, Mega Siska Musiam Syifa Auliani Syifa Auliani Vitalika Regitasari Wahyuni, Amaliyah Winsa Wira Wijaya Yusrinie Wasiaturrahmah