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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana SIMBIOSIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Mikologi Indonesia
Meitini Proborini Wahyuni
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana. Jl. Raya Kampus UNUD, Jimbaran, Kuta Selatan, 80361, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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EFEKTIVITAS EKSTRAK KULIT BATANG AMPUPU (Eucalyptus alba Reinw. Ex. Blume) DALAM MENGHAMBAT PERTUMBUHAN JAMUR Fusarium sp. PENYEBAB BUSUK TONGKOL JAGUNG (Zea mays L.) Bernadina Metboki; Ni Putu Adriani Astiti; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p01

Abstract

The fungal-related diseases were responsible as one of many reasons for low production of maize in Indonesia. Controlling fungal contaminants using chemical is hazardous for human nowadays. Thus the need of environmental friendly vegetative-based fungicide such as from the extract of Ampupu tree bark is important. The objective of this study was to investigate the antifungal effect from Ampupu tree bark crude extract in response to growth of Fusarium sp, which responsible as the causal agent of corncob rot. The inhibition test on growth of Fusarium sp. by Ampupu tree bark crude extract was performed with diffusion well method on PDA as media. The process involved the infusion of crude extracts at 1.5%, 3.0% and 4.5% concentration, respectively, into diffusion well on each Petri disk. The result showed that by in vivo treatment, crude extract of Ampupu tree bark with concentration of 1.5%, 3,0% and 4.5%, were able to inhibit the fungal growth of Fusarium miniliforme as the causal agent of corncob rot with diameter of inhibition zone 0.18 mm, 1.85 mm and 2.01 mm, respectively. The higher the concentration of the crude extract given, the larger the diameter of inhibition zone formed.
Isolasi, Identifikasi dan Persentase Keberadaan Hifa Jamur Endofit pada Tanaman Gemitir (Tagetes erecta L.) di Beberapa Daerah di Bali Bekti Revi Rahayu; Meitini Wahyuni Proborini; Ida Bagus Gede Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p12

Abstract

Endophytic fungi are found in roots, stems, leaves, flowers, fruit, until rhizosphere in soil area, where the fungus is widely used as an antifungal, biostimulan, antibacterial, and many more. Gemitir is one plant which widely cultivated in some areas in Bali. In Bali this plants use as a material for upakara, ornamental plants and herbs. This study was conducted to determine the type and different kind of endophytic fungi and to see the percentage of endophytic fungal hyphae in the roots, stems, and leaves on gemitir which taken at different locations. Sampling was taken at the Pangsan-Badung, Angseri-Tabanan Regency and Temesi-Gianyar. The sampling method which used is the diagonal method with five sample points. Isolation and identification process was carried out in the Laboratory of Plant Taxonomy (Mycology), State University of Udayana in January-March 2017. In this study of 15 species successfully identified to the genus level that belongs to the six genera namely Alternaria, Aspergillus, Botrytis, Trichoderma, Penicillium and Syncepalastrum. Total of fungal colonies in most samples were found at research location A (Pangsan-Badung Village) and percentage of highest endophytic fungal hypha existed at location C (Temesi-Gianyar Village).
EKSPLORASI DAN IDENTIFIKASI JENIS-JENIS JAMUR KLAS BASIDIOMYCETES DI KAWASAN BUKIT JIMBARAN BALI Meitini W. Proborini
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Ekplorasi jamur-jamur klas Basidiomycetes menggunakan metode jelajah selama musim penghujan telah dilakukan di kawasan Bukit Jimbaran selama enam bulan (Desember 2005 - Mei 2006).  Hasil penelitian telah ditemukan sebanyak 30 spesimen jamur-jamur makroskopis.  Hasil identifikasi dan karakterisasi diperoleh 18 spesies dan dua isolat spesimen yang termasuk klas Basidiomycetes dan 10 spesimen merupakan  Ascomycetes. Jamur-jamur yang ditemukan banyak terdapat pada tanah serasah dan pohon-pohon atau kayu-kayu yang telah lapuk.
Aplikasi fungi mikoriza arbuskula Glomus sp. dan Trichoderma sp. sebagai pupuk hayati dan biostimulator pertumbuhan tanaman tomat (Solanum lycopersicum L.) Anak Agung Yulia Anggiani; Meitini Wahyuni Proborini; I Ketut Muksin; Inna Narayani
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p02

Abstract

Tomato (Solanum lycopersicum L.) is a horticultural crop that has many benefits and potential to be developed. Market demand for tomatoes is increasing, but tomato production in Bali has decreased due to biotic and abiotic factors. One of efforts to increase tomato production is by using the Mycorrhizal Arbuscular Fungi (FMA) Glomus sp. and Trichoderma sp. which act as a biofertilizer and biostimulator. The study was conducted in November 2020 until February 2021 and aims to analyze the effect of AMF, Trichoderma, and combinations on the growth of tomato plants. Research site was at the Greenhouse and the Laboratory of Plant Taxonomy in the Department of Biology Faculty of Mathematics and Natural Sciences Udayana University. The experiment used factorial Randomized Block Design (RBD) with two factors, three replicates. First factor was the dose of AMF Glomus (0, 100, 150, and 200 spores per plant) and the second factor was the dose of Trichoderma (0, 10, and 20 mL per plant). Data of this study was analyzed using Analysis of Variance (ANOVA) followed by Duncan Multiple Range Test (DMRT). The result showed that AMF Glomus 150 spores increased plant height, root length, number of flower bunches, dry plant mass, and obtained the highest AMF colonization percentage at 35% (medium category).
TOTAL BAKTERI PADA JAMU TRADISIONAL DI PASAR KEDONGANAN KELURAHAN JIMBARAN KABUPATEN BADUNG PROVINSI BALI Putu Ayu Sukmawati; Meitini W. Proborini; Retno Kawuri
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis cendawan, total koloni bakteri, dan mengetahui keberadaan bakteri pencemar Escherichia coli yang terdapat pada jamu tradisional di Pasar Kedonganan. Sampel diambil dari 4 pedagang jamu, setiap pedagang diambil 4 sampel jamu yaitu jamu beras kencur, sirih, kunyit dan sambiloto. Perhitungan Total koloni cendawan dan total koloni bakteri pada jamu dilakukan dengan menggunakan metode pengenceran dan total koloni bakteri secara statistik dianalisa dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa 6 jenis cendawan yaitu: Aspergillus niger, A. flavus, Penicillium citrinum, P. digitatum, P. brevicompactum, dan Acremonium sp ditemukan pada jamu. Total jumlah koloni cendawan tertinggi ditemukan pada jamu beras kencur (107x105 CFU/ml) sedangkan terendah ditemukan pada jamu kunyit (20,5 x 105 CFU/ml). Batas standar kandungan jamur pada makanan yang direkomendasikan oleh Departemen Kesehatan RI adalah sebesar < 104 CFU/ml. Total bakteri tertinggi ditemukan pada jamu beras kencur (267,6x108 CFU/ml), sedangkan terendah ditemukan pada jamu kunyit (39x108CFU/ml). Kandungan bakteri juga melampaui standar dari Departemen Kesehatan RI Keseluruhan jamu telah diuji telah melampaui ambang batas Departemen Kesehatan RI yaitu sebesar < 106 CFU/ml. E.coli di temukan jamu sambiloto, jamu kunyit, jamu beras kencur.  Oleh karena itu kehati-hatian perlu dilakukan jika meminum jamu.
PENGARUH MEDIA TERHADAP PERTUMBUHAN DAN BIOMASSA CENDAWAN Alternaria alternata (Fries) Keissler Pinki Prahara Taurisia; Meitini W Proborini; Irsan Nuhantoro
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Alternaria alternata merupakan salah satu cendawan patogen. Pertumbuhan dan biomassa cendawan A. alternata sangat dipengaruhi oleh pH dan nutrisi yang terkandung pada media tumbuh. Jenis media yang paling sesuai dapat memacu pertumbuhan cendawan. Penelitian ini bertujuan untuk mengetahui pengaruh media yang berbeda terhadap pertumbuhan dan biomassa cendawan A. alternata. Penelitian dilaksanakan di Laboratorium Taksonomi Tumbuhan (Mikologi) Jurusan Biologi Fakultas MIPA, Universitas Udayana dan Balai Karantina Pertanian Kelas I Denpasar. Cendawan ditumbuhkan menggunakan tujuh media yang berbeda (PDA, PCA, SDA, CDA, CMA,NA, MEA). Rancangan yang digunakan adalah Rancangan Acak Lengkap, setiap perlakuan diulang sebanyak empat kali. Pengukuran dilakukan pada hari keempat, kelima, keenam, dan ketujuh. Pengukuran berat biomassa dilakukan dengan pengovenan selama 5 hari. Hasil pertumbuhan koloni A. alternata pada media CDA (5,58 mm) berbeda nyata dengan media PDA (5,50 mm) dan PCA (5,28 mm), sedangkan pengukuran berat biomassa terbesar yaitupada media PCA (0,470 mg) dan terendah pada media NA (0,047 mg).
Arbuscular mycorrhizal fungi (AMF) Glomus spp. as bio-control of Fusarium oxysporum Schlecht et Fr. infection in chilli plants (Capsicum frutescens L.) Ni Putu Muni Raisani; Meitini Wahyuni Proborini; Ni Luh Suriani; Eniek Kriswiyanti
Jurnal Biologi Udayana Vol 24 No 1 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.014 KB) | DOI: 10.24843/JBIOUNUD.2020.v24.i01.p05

Abstract

Fusarium oxysporum Schlecht et Fr. merupakan patogen tular tanah yang dapat menyebabkan layu fusarium pada cabai rawit. Penggunaan Fungi Mikoriza Arbuskula (FMA) sebagai simbion dapat meningkatkan ketahanan tanaman terhadap infeksi jamur pathogen F.oxysporum. Penelitian ini bertujuan untuk mengetahui pengaruh FMA sebagai biokontrol pertumbuhan tanaman cabai rawit terhadap infeksi F. oxysprum serta efektivitas pemberian FMA untuk menurunkan intensitas serangan penyakit layu fusarium. Bibit cabai rawit ditumbuhkan pada media tanah steril (kontrol negatif), tanah steril dan 10 ml patogen (kontrol positif), tanah steril dan FMA 100 g, FMA 100 g dan 10 ml patogen, tanah steril dan 200 g FMA, FMA 200 g dan 10 ml patogen, tanah steril dan 300 g FMA dan 10 ml patogen. Hasil penelitian menunjukkan bahwa pemberian 200 g spora FMA menunjukkan pengaruh berbeda nyata pada tinggi tanaman, jumlah daun, berat kering akar dan berat kering tajuk tanaman cabai rawit berumur tiga bulan. Sedangkan inokulasi 300 g FMA memberikan hasil terbaik pada buah cabai dan persentase kolonisasi FMA sebesar 84, 45%. Inokulasi 300 g FMA menurunkan infeksi F. oxysporum saat 30 Hari Setelah Tanam (HST) sebesar 3.36%. Kesimpulan dari riset ini menunjukkan inokulasi FMA Glomus spp. mampu meningkatkan tinggi tanaman, jumlah daun, berat kering akar dan berat kering tajuk, kecepatan berbunga dan ketahanan tanaman terhadap infeksi jamur F. oxysporum.
ISOLASI DAN IDENTIFIKASI JAMUR PENYEBAB PENYAKIT LAYU DAN ANTAGONISNYA PADA TANAMAN KENTANG YANG DIBUDIDAYAKAN DI BEDUGUL, BALI Ida Ayu Putu Suryanti; Yan Ramona; Meitini W. Proborini
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.07 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui jamur penyebab penyakit layu pada tanaman kentang dan mengisolasi antagonisnya dari daerah rhizosphere tanaman tersebut yang dibudidayakan di Desa Candikuning, Bedugul, Bali. Untuk mendeteksi patogen penyebab penyakit tersebut, pada penelitian diterapkan Postulat Koch. Hasil penelitian menunjukkan bahwa Fusarium oxysporum isolat A dan B merupakan dua isolat yang terindikasi sebagai penyebab penyakit layu pada tanaman kentang. Pada penelitian ini tiga isolat jamur antagonis (Trichoderma spp. isolat A, Trichoderma spp. isolat B, dan Aspergillus niger) berhasil diisolasi dari daerah rhizosphere tanaman kentang. Uji antagonis (in vitro) dengan menggunakan dual culture assay menunjukkan semua antagonis menghambat pertumbuhan jamur patogen Fusarium dengan persentase hambatan yang bervariasi antara 36,57 - 75,76%. Trichoderma spp. isolat A menunjukkan hasil terbaik dalam menghambat pertumbuhan jamur patogen, dengan persentase hambatan sebesar 75,76%. Sementara itu, persentase hambatan terkecil (36,57%) pada patogen teramati pada Aspergillus niger.
IDENTIFICATION OF MICROBES ANTAGONISTIC AGAINST Fusarium oxysporum ISOLATED FROM RHIZOSPHERE ZONE OF WATERMELON Anak Agung Ngurah Nara-Kusuma; Yan Ramona; Meitini Wahyuni Proborini
Jurnal Biologi Udayana Vol 22 No 2 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.834 KB) | DOI: 10.24843/JBIOUNUD.2018.v22.i02.p04

Abstract

This research was aimed to isolate and identify microbes which antagonistic against Fusarium oxysporum, the causative agent of vascular wilt in watermelon plants. The antagonistic microbes were isolated from soil samples collected from rhizosphere of watermelon farm located at west Sanur village, South Denpasar, Bali. Isolation of fungi and bacteria were conducted on potato dextrose agar medium (PDA) and nutrient agar medium (NA), respectively. Fungal isolates were then observed under light microscope for its morphological characteristics before identification using a reference book. Bacterial isolates were characterized using various tests, such as gram stain reaction, existence of endospores, catalase reaction, and ability to ferment various sugars. Their characteristics were then compared with those described in a reference book. Two fungal species (Trichoderma harzianum and Trichoderma viride) and two bacterial antagonists (Pseudomonas sp. and Bacillus sp.) were found to have potential to be developed as biocontrol agents to inhibit the growth of F. oxysporum.
UJI KEMAMPUAN SPORA CENDAWAN MIKORIZA ARBUSKULA (CMA) LOKAL BALI PADA PERTUMBUHAN TANAMAN KEDELAI (Glycine max L.) Risha Masfufah; Meitini W. Meitini W. Proborini; Retno Kawuri
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Necessity of soybean in Indonesia increase every year along with population growth. The goverment need to importthe soybeans from abroad. Soybean in Indonesia are generally planted in ricefield and upland (dryland) which have potentialof deficiency water. Water deficiency can caused the reduction of soybean production. Therefore some alternatives are neededto increase the yield of soybean productivity, one of them is applying arbuscular mycorrhizal fungi (AMF). The aim of thisresearch was to know the dosage of isolates AMF to increase the growth of soybean. The experiments conducted over 2months at Agriculture Faculty’s Greenhouse, Udayana University. The research use Completely Randomized Design whichconsists of 5 treatments, which are : without inoculation/negative control (M0), 50 spores AMF (M1), 100 spores AMF (M2),150 spores AMF (M3), and ZA/positive control (M4). The results showed that, the inoculation of spores AMF Bali indigenuswere significantly different (P<0,05) on the number of leaves, leaf length, root fresh weight, and percentage of rootcolonization. The parameter of plant height, leaf width, plant fresh weight, and plant dry weight are no significant different(P>0,05) based on analysis of variance (ANOVA) level 5%. Doses 50 spores AMF (M1) is the dose of inoculants has beenable to increase number of leaves, leaf length, root fresh weight, and percentage of root colonization.Keywords : fungi mycorrhizal arbuscular, mycorrhiza doses, soybean.
Co-Authors Anak Agung Devina Asana Putri Anak Agung Gde Raka Dalem Anak Agung Ngurah Nara-Kusuma Anak Agung Yulia Anggiani Bekti Revi Rahayu Bernadina Metboki Deny Suhermawan Yusup Dewa Ayu Andriastini Dewa Ngurah Suprapta ENIEK KRISWIYANTI Gustu Widi Putra I Gede Sugita Artana I Ketut Junitha I KETUT MUKSIN I Ketut Sundra I MADE ADNYANA IDA AYU ASTARINI Ida Ayu Putu Suryanti Ida Bagus Gede Darmayasa Ida Bagus Gede Daymayasa Inna Narayani Irsan Nuhantoro Job Nico Subagio Junita Hardini Krisdayani, Putu Mita Lila, Kadek Dian Lila Sawitri Kumala Lilis Riana Tambunan Lily Windasari Made Mega Yuliasari Made Ria Defiani MADE SUSUN PARWANAYONI Maghfirotun Nisa Muhamad Masyrur Nadya Treesna Wulansari Naomi Anggi Triarta Ni Luh Desy Wulandari Ni Luh Suriani Ni Made Dewi Wahyuni Ni Putu Adriani Astiti Ni Putu Muni Raisani Ni Wayan Desi Bintari Pinki Prahara Taurisia Putu Anggan Pradipta Utama Putu Ayu Sukmawati Putu Shinta Devi Retno Kawuri Risha Masfufah Riswanda Dwiky Priyanta Sukmawati Sukmawati Sukmawati Sukmawati Tadzkia Hanifah Akbar Wijayanti, Febri Eka Wira Purnama Santi Yan Ramona