Meitini Proborini Wahyuni
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana. Jl. Raya Kampus UNUD, Jimbaran, Kuta Selatan, 80361, Bali

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Journal : SIMBIOSIS

UJI KEMAMPUAN SPORA CENDAWAN MIKORIZA ARBUSKULA (CMA) LOKAL BALI PADA PERTUMBUHAN TANAMAN KEDELAI (Glycine max L.) Risha Masfufah; Meitini W. Meitini W. Proborini; Retno Kawuri
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Necessity of soybean in Indonesia increase every year along with population growth. The goverment need to importthe soybeans from abroad. Soybean in Indonesia are generally planted in ricefield and upland (dryland) which have potentialof deficiency water. Water deficiency can caused the reduction of soybean production. Therefore some alternatives are neededto increase the yield of soybean productivity, one of them is applying arbuscular mycorrhizal fungi (AMF). The aim of thisresearch was to know the dosage of isolates AMF to increase the growth of soybean. The experiments conducted over 2months at Agriculture Faculty’s Greenhouse, Udayana University. The research use Completely Randomized Design whichconsists of 5 treatments, which are : without inoculation/negative control (M0), 50 spores AMF (M1), 100 spores AMF (M2),150 spores AMF (M3), and ZA/positive control (M4). The results showed that, the inoculation of spores AMF Bali indigenuswere significantly different (P<0,05) on the number of leaves, leaf length, root fresh weight, and percentage of rootcolonization. The parameter of plant height, leaf width, plant fresh weight, and plant dry weight are no significant different(P>0,05) based on analysis of variance (ANOVA) level 5%. Doses 50 spores AMF (M1) is the dose of inoculants has beenable to increase number of leaves, leaf length, root fresh weight, and percentage of root colonization.Keywords : fungi mycorrhizal arbuscular, mycorrhiza doses, soybean.
BIOKONTROL ENDOMYCORRHIZA AGAINST FUNGUS Curvularia sp. THE CAUSE OF DISEASE ON CORN (Zea mays L.) Lily Windasari; Meitini Wahyuni Proborini; Made Ria Defiani
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.257 KB) | DOI: 10.24843/JSIMBIOSIS.2019.v07.i02.p03

Abstract

In Indonesia, production of corn are used for fodder (55%), food (30%), and seed (15%). The growth of corn plants is limited by the presence of pests, weeds, and plant diseases. Curvularia sp. causing leaf spot and could decrease corn production. Use of mycorrhiza can be used as a biological agent in preventing pathogens outbreaks. Isolates of Curvularia sp. fungal was sprayed on corn seed. This research was conducted in laboratorium and green house. Corn seedlings were grown on sterile soil media (control), mycorrhizal treatments (100g of mycorrhizal + Curvularia sp., 200g of mycorrhizal + Curvularia sp., 300g of mycorrhizal + Curvularia sp.) and Curvularia sp. The observed variables were plant height, number of leaves, rate of disease progression, fresh weight and dry weight of plant canopy. The results showed that application of 100g inoculum tend to increase plant growth to 26-27% and percentage of mycorrhizal colonization to 58,66% thereof can decrease pathogen infection on plant at 28 day after planting.
EKSPLORASI SPASIAL CENDAWAN TANAH PADA SEKITAR RHIZOSFER TANAMAN JAMBU METE (Anacardium occidentale L.) DI KARANGASEM DAN BULELENG-BALI Ni Luh Desy Wulandari; Meitini W. Proborini; I Ketut Sundra
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Research have been carried out to calculate total colony of fungi, determine fungispecies, and the rate spread of the fungi in rhizosphere a cashew plantations (Anacardiumoccidentale L.) at Sukadana Karangasem and Sendang Buleleng for 4 months (October 2012- January 2013). Samples were taken from two different areas in which five point weresampled. Results data were analyzed qualitatively by identifying the species of fungi that is adescription. The quantitative data was taken to calculate total colony of fungi. The researchfound 11 species of fungi taken from two different locations, namely: Aspergilus flavus, A.niger, A. parasiticus, Botrytis cinera, Cladosporium sphaeospermum, Fusariumsporotrichioides, P. brevicompactum, P. citrinum, P. chrysogenum, Rhizopus stolonifer, andSynchephalastrum racemosum. Total fungal colony in Sukadana Karangasem was : 33.6 x103 - 35 x 103 CFU /g, in Sendang Buleleng 22.6 x 103 – 37.6 x 103 CFU/g. Abundance offungi in Sukadana Karangasem is relatively uniform (0.85) and in Sendang Buleleng belongto cluster (1,03).Keywords: Cashew rhizosphere, abudance of fungal species, Karangasem, Buleleng-Bali
DAYA HAMBAT EKSTRAK KASAR DAUN KALIANDRA (Calliandra calothyrsus Meissn.) TERHADAP JAMUR KONTAMINAN PADA PAKAN KONSENTRAT AYAM RAS PEDAGING I Gede Sugita Artana; IB.G Darmayasa; Meitini W. Proborini
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

ABSTRACT This aims of the study were to determine the species of fungi contaminated chicken broiler consentrate and to find out the inhibition of crude extract's leaves of caliandra. The samples were taken in Selat, Karangasem. Maceration method was conducted to extracted leaves of caliandra, disk diffusion agar method was conducted to find out the inhibition of crude extract leaves of caliandra. Result showed that there were six types of fungi on chichken broiler consentrate such as Aspergilus flavus, Aspergilus niger, Aspergillus fumigatus, Penicillium brevicompactum, Penicillium candidum dan Trichoderma viridae. The result also showed that 100.000 ppm extract concentrate inhibit Aspergillus flavus growth on 12.72 ± 0,27 mm. Keywords : fungi, inhibition strength, crude extract’s, caliandra, Aspergillus flavus
EKSPLORASI SPATIAL DAN IDENTIFIKASI CENDAWAN ENDOFIT PADA TANAMAN KAKAO (Theobroma cacao L.) DI BALI Lilis Riana Tambunan; Meitini Proborini; Putu Adriani Astiti
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.428 KB) | DOI: 10.24843/JSIMBIOSIS.2018.v06.i01.p01

Abstract

Cocoa plant (Theobroma cacao L.) can grow well in the highlands and lowlands, a plantation and industrial plantwhich is known as one of the export commodities, as raw material for the food and pharmaceutical industries which cancontribute to an increase in foreign exchange endophytic fungus is a fungus living on a live network of plants such asseeds, leaves, flowers, twigs, stems and roots. Truffles are generally mutualistic endophyte and is known to stimulate thegrowth of plants, capable of producing antibiotics and plant growth regulators (hormones). This study aims to determinethe diversity and identify endophytic fungi on Kakao.yang found in two different locations in Bali, in the village of KajaBuahan District of Payangan, Gianyar and the Village of Tua, Marga, Tabanan regency subdistricts. Research carried outin the field with the exploration methods, isolation, identification and diversity analysis conducted at the Laboratory ofPlant Taxonomy (Mycology), Department of Biology, Faculty of Mathematics, University of Udayana. Laboratoryresearch conducted by isolating the roots, leaves, plant stems and content of the soil (rhizosphere) cocoa. Once isolated,grown on PDA medium subsequently incubated at room temperature 250C. Isolates that grow back isolated and purifiedon a PDA medium for further identified to the genera or species level. A total of six isolates of endophytic fungi identifiedin the rhizosphere and cocoa plant that is where four of these isolates were identified to species, two isolates were notidentified until genus. Fungi were identified namely Aspergillus flavus, Rhizopus stolonifer, Cladosporiumsphaeropermum, Trichoderma viride, isolates 1 and 2 isolates.Keywords: cocoa, endophytic fungi, highlands and lowlands