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Journal : Biospecies

Aktivitas Ekoenzim Nanas (Ananas comosus L. Merr.) Var. Queen Sebagai Antimikosis Dermatofita (Trichophyton rubrum): Ecoenzyme Activity of Queen Variety Pineapple (Ananas comosus L. Merr.) as Antimycosis Dermatophytes (Trichophyton rubrum) Zahira, Saroci Dorratul; Ihsan, Mahya; Maritsa, Hasna Ul
Biospecies Vol. 16 No. 1 (2023): Januari 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i1.21096

Abstract

Ecoenzyme is a type of product made from fermented fruit/vegetable waste that can be used as an antimycosis. chemical compounds and acetic acid has the ability as an antimicrobial. Trichophyton rubrum is a type of dermatophyte fungus that can cause chronic dermatophysis. This fungus is very easy to grow in humid conditions. The purpose of this study was to determine the ability of pineapple ecoenzymes from Tangkit as antimycosis against Trichophyton rubrum fungus, to determine the antimycosis activity of pineapple ecoenzymes from Tangkit in inhibiting T. rubrum fungi and to determine the content of compounds in pineapple ecoenzymes from Tangkit. The test of the inhibition of this ecoenzyme was carried out by the disc diffusion method. The treatments in the test were positive control (ketoconazole), negative control (aquadest), and ecoenzyme concentrations of 50%, 75% and 100%. Data analysis used ANNOVA and continued with Duncan's test. The results showed that the ecoenzyme could be used as an antimycotic against T. rubrum. 50% ecoenzyme concentration was the best test concentration in inhibiting the growth of T. rubrum with a large inhibition zone formed of 14,822. The compounds contained in the ecoenzyme are tannins, saponins and bromelain Keywods: Trichophyton Rubrum, Pineapple Ecoenzyme, Antimycosis, Tangkit Pineapple, Dermatophytes   Abstrak Ekoenzim adalah jenis produk yang terbuat dari limbah dari buah/sayur yang difermentasi yang dapat digunakan sebagai antimikosis. Trichophyton rubrum merupakan salah satu jenis jamur dermatofita yang dapat menyebabkan dermatofisis kronis. Jamur ini sangat mudah tumbuh pada kondisi yang lembab. Kandungan senyawa kimia dan asam asetatnya memiliki kemampuan sebagai antimikroorganisme. Tujuan dari penelitian ini adalah Mengetahui kemampuan ekoenzim nanas dari Tangkit sebagai antimikosis terhadap jamur Trichophyton rubrum, mengetahui nilai aktivitas antimikosis ekoenzim nanas dari Tangkit dalam menghambat jamur Trichophyton rubrum dan mengetahui kandungan senyawa pada ekoenzim nanas dari Tangkit. Pengujian daya hambat ekoenzim ini dilakukan dengan metode difusi cakram. Perlakuan dalam uji yaitu kontrol positif (ketoconazole), kontrol negatif (akuades), serta konsentrasi ekoenzim 50%, 75% dan 100%. Analisis data menggunakan ANNOVA dan dilanjutkan dengan uji Duncan.Hasil menunjukkan bahwa ekoenzim dapat digunakan sebagai antimikosis terhadap T. rubrum. Konsentrasi ekoenzim 50% merupakan konsentrasi uji terbaik dalam menghambat pertumbuhan T. rubrum dengan besar zona hambat yang terbentuk yaitu 14,822. Senyawa yang terdapat pada ekoenzim yaitu tannin, saponin dan bromelin. Kata Kunci: Trichophyton rubrum, Ekoenzim Nanas, Antimikosis, Nanas Tangkit, Dermatofita
AKTIVITAS EKOENZIM KULIT NANAS (Ananas comosus (L). MERR) VARIETAS TANGKIT SEBAGAI ANTISEPTIK ALAMI TERHADAP Escherichia coli DAN Staphylococcus aureus Pratama, Hefzi Prasetyo Azri; Syah, Putri Indriaty; Royhan, Ibnu; Maritsa, Hasna Ul; Yusuf, Ashif Irvan
Biospecies Vol. 18 No. 1 (2025): Januari 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i1.36701

Abstract

Bacteria are one of the causes of disease in humans, there are various types of bacteria in the environment that can infect humans either directly or by vectors. Escherichia coli and Staphylococcus aureus bacteria are bacteria that are often found in everyday life. One effective effort to fight these bacteria is to wash hands with cleaning agents. One of the natural cleaners that can be used as hand soap is ecoenzyme from pineapple skin because it contains antibacterial substances such as vitamin C, Carotenoids and Flavonoids. In addition, pineapple skin also contains tannins, saponins, phenols, carbohydrates, terpenoids, alkaloids, phenols, anthraquinones, and amino acids. In addition to having a rich content of secondary metabolite compounds, pineapple skin is also not utilized so that pineapple skin waste is widely found. This makes pineapple skin waste very suitable to be processed into ecoenzymes and used as antibacterial cleaning agents. The purpose of this study was to determine the ability of pineapple ecoenzyme to inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria and to determine the effectiveness value of pineapple ecoenzyme in inhibiting the growth of Escherichia coli and Staphylococcus aureus bacteria. The method used in this study was the test of ecoenzyme inhibition activity against Escherichia coli and Staphylococcus aureus bacteria using discs. There were 4 treatments, namely 25%, 50%, 75%, 100% ecoenzyme and 4 replications were made. Based on the results of the study, it is known that the effectiveness of pineapple peel waste ecoenzyme has a weak category with the highest concentration inhibiting Escherichia coli bacteria of 4.75 mm at a concentration of 100%, while Staphylococcus aureus bacteria had 2.75 mm at a concentration of 75%. The 70% alcohol control has an inhibitory power against both bacteria which is still higher than Escherichia coli and Staphylococcus aureus bacteria.