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All Journal ODONTO Dental Journal JURNAL PENGABDIAN KEPADA MASYARAKAT Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Odonto dental journal Surabaya Biomedical Journal Indonesian Journal of Community Empowerment Surabaya Biomedical Journal
Rima Parwati Sari, Rima Parwati
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : ODONTO Dental Journal

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In Vitro Study of Calcium Hydroxide Microencapsulation with Stichopus hermanii as Pulp Capping Material Rochyani, Linda; Sari, Rima Parwati; Rizal, Moh Basroni
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.198-207

Abstract

Background: Calcium hydroxide is a material that is widely used in pulp capping treatment. However, the use of this material causes tunnel defects due to the large absorption of calcium. This can be manipulated by creating an encapsulation formula. Stichopus hermanii is a marine biota that contains lots of hyaluronic acid and can be used as an encapsulation material which also plays a role in cell regeneration. The characteristics of the microencapsulated calcium hydroxide preparation with Stichopus hermanii as pulp capping material through an in vitro study.Method: Stichopus hermanii was made freeze dry and dissolved with 1% acetic acid to obtain a gel concentration of 0.2%, 0.4%, and 0.8%. Next, calcium hydroxide powder is added with a ratio of 1: 6 and tripolyphospate (TPP) is added as a cross-linker agent and the mixture is stirred until homogeneous. The test material formed was subjected to SEM, PSA and cytotoxicity tests.Result: The cytotoxicity test showed that all of the test materials (0.2%; 0.4%; 0.8%) were not toxic, especially at a concentration of 0.2%. The PSA test results indicate that this encapsulation shows the micro particle size formed. The SEM test shows a rough and stringy surface shape, where at a concentration of 0.2%, a smoother morphological formation is found.Conclusion: The in vitro study showed that the microencapsulation of calcium hydroxide with Stichopus hermanii as a pulp capping material showed the characteristics of the non-toxic material, the micro size and the rough and fibrous surface shape.
Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Prananingrum, Widyasri; Caroline, Grace; Rizal, Mohammad Basroni; Prabowo, Puguh Bayu; Pratama, Afif Fahwi; Resaldi, Muhammad Firdan; Annisa, Nindya Yuanita; Fadhilah, Yusti; Sari, Rima Parwati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Abstract

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200˚C for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ø6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ø7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.
Co-Authors Agustia, Lita Annisa, Nindya Yuanita Aprilia Aprilia, Aprilia Apsari, Anindita Ariestania, Vivin Arya Brahmanta, Arya Caroline, Grace Chiquita Prahasanti Chondro, Ronaldo Triputra Damaiyanti, Dian Widya Dian Mulawarmanti, Dian Dwi Andriani, Dwi Dwi Budi Santoso Dwi Setianingtyas Eddy Hermanto Elidasari, Monica Elidasari, Monika Endah Wahjuningsih, Endah Fadhilah, Yusti Fauzia, Bunga Fitri, Karimatul Fitria Rahmitasari Hadinata, Yongki Handayani, Budi Hollanda, Ghita Hadi Indarti, Caecilia Ira Arundina Karsini, Isidora Kharinna Widowati, Kharinna Kristanti Parisihni Kurniawan, Hansen Kusumawardani, Chaterina Diyah Nanik Kusumawardani, Chaterina Dyah Nanik Laihad, Fanny M. Lestari, IGAM Oka Linda Rochyani, Linda Matsuyama, Miwa Megantara, Rizko Wira Artha Meinar Nur Ashrin Nafi'ah Nanik, Chaterina Diyah Nanik, Chaterina Diyah Nike Hendrijantini Parasihni, Kristanti Pargaputri, Agni Febrina Pratama, Afif Fahwi Pratiwi, Safira Agnes Priambodo, Nur Tsurayya Puguh Bayu Prabowo, Puguh Bayu Ramanindia, Andiani Resaldi, Muhammad Firdan Retno Pudji Rahayu Rizal, Moh Basroni Rizal, Mohammad Basroni Rizka, Yoifah Saptaswari, Dianty Sari, Maharani Dewinta Soedjarwo, Sri Agoes Soesilowati, Pratiwi Sugiarto Budijono, Stevanus Candra Susanto, Michelle Rosalind Widaningsih Widyasri Prananingrum WIDYASTUTI Yufita Fitriani Yulie Emilda, Yulie Yusuf, Diajeng Ayu Permatasari