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Journal : Medical Laboratory Technology Journal

Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte Puspitasari Puspitasari; Evi Rinata; Rohman Dijaya; Siska Aprilia; Dina Trikumalasari; Livia Nur Azzah; Qilmia Fanani; Miftahul Mushlih; Andika Aliviameita; Dian Delta
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.348 KB) | DOI: 10.31964/mltj.v5i2.229

Abstract

Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p
Co-Authors Abdillah, David Fahmi Achmad Alfi Fakhresi Achmad Ariq Rivaldhi Ade Eviyanti Agung Novianto, Putra Agung Wijaya Aini, Della Swastika Nur Ali Akbar Ambarwati , Rita Andika Aliviameita Ardhian Ramadhan Arif Senja Fitrani Bagus Dwi Yulianto Bakta , Nabila Ayu Budi, Bagus Setyo Busono, Suhendro Cholifah Darlis Herumurti Deni Sutaji Dian Delta Dimas Ayu Putri Risky Nofa Dina Trikumalasari Diva Putri Anasya Eka Fatra Arif Hidayatullah Eko Agus Suprayitno Erfina, Idha Maharani Evi Rinata Fatona Kholifatun Nisa' Febrian Endi Pranyoto Fiolitta , Anggi Firdaus Margo Utomo Firman Milani Gracia, Aisyah Silvia Hamzah Setiawan Harunur Rosyid Hatuwe, Razif Zulvikar Hibatullah Putra, Dimas Radito Idha Maharani Erfina Ika Ratna Indra Astutik Ilham Basri K Irwan Alnarus Kautsar Julianata, Akhsan Maula Khotiah, Titiek Kusuma, Selvia Ferdiana lambang , Yanuangga Galahart Livia Nur Azzah M. Ryas Hakim Mauliana, Metatia Intan Miftahul Mushlih Mochamad Alfan Rosid Mudji Astuti Muhammad Fachruddin, Muhammad Muhammad Iqbal Al Mulki Nanik Suciati Nanik Suciati Ningsih, Maudina Fatika Novia Ariyanti Nurfirmansyah, Agustin Nuril Lutvi Azizah Nurwijayanti Octavia, Elga Prastiyanti, Erika Priyo Wahyu Setiyo Aji Puspitasari Puspitasari Putra, Riski Firmansyah Wilis Putri, Adelia Kharisma Qilmia Fanani Rahmawati, Indah Agustinah Ratri Enggar Pawening Retna Fetty Idamayanti Rhomadiniyah, Dewi Rina Safitri Rita Ambarwati Rivaldhi, Achmad Ariq Rizky Bagas Prasetyo, Achmad Rizky Bayu Wardana S Suprianto Sahera, Charisa Nur Sandi Wahyu Maulana Setiawan, Hamzah Shovi Firdaus Siska Aprilia ST, M. Atho'illah Suhendro Busono Sumarno . Sumarno Sumarno Suprianto Suprianto Suprianto Suprianto Suprianto Suprianto Suroiyah, Lailatul Surur, Moch Shobachus Syahgita, Mico Hari Tasya Gusti Amalia Taurusta, Cindy Uce Indahyanti Umami, Nur Reza Umi Khoirun Nisak Wahyu Nur Islamudin Wahyu Setia Bintara Wardana, Rizky Bayu Wardani, Dwi Sukma Yansah, Muhammad Kahfi Yulian Findawati Yunianita Rahmawati, Yunianita Yunita Setiyaningsih Yusuf Raharja Yusuf, Farid Maulana Yuwanto, Mahmud Adi