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All Journal Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Indonesian Journal of Pharmaceutical Science and Technology Pro-Life Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Media Akuakultur Molekul: Jurnal Ilmiah Kimia Hanifiya: Jurnal Studi Agama-Agama Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Madihah Madihah

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran

Author-ID : 604982

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Veterinary

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PENGARUH RENDAMAN KAYU ANGIN (Usnea baileyi) DALAM NIRA SEGAR DAN NIRA REBUS TERHADAP HATI TIKUS (Rattus norvegicus) YANG TERPAPAR TIMBAL ASETAT Desak Made Malini; Iin Supartinah; Madihah Madihah; Andi Perdana
Jurnal Pro-Life Vol. 6 No. 3 (2019): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Lead acetate inhaled caused oxidative damage of liver due to the formation of free radicals. Ones of the plant thathas a high antioxidant content is Usnea baileyi. This study aims to examine the potency of fresh and boiled U.baileyi to reducing rats liver damage induced by lead acetate. This study used randomized complete design withnine treatments and three replications. Twenty seven male rats were randomly divided into 9 groups namely onepositive control group (given 100 mg/kb bw lead acetate), two negative control groups (given 1 ml fresh sap and 1ml boiled sap), three groups given U. Baileyi immersion treatment in fresh sap and three group treated U.Baileyi immersion in boiled sap with dose of 3,500 mg/kg BW, 6,000 mg/kg BW and 10,500 mg/kg BW afterinduced by 100 mg/kg BW lead acetate. Treatment was given orally for 15 consecutive days The results showedthat the morphology of rats treated with U. baileyi dosages of 6,000 mg/kg bw and U. baileyi 10,500 mg/kg b wboth in fresh sap and boiled sap had morphology that was not significantly different from negative controls.Whereas in the histological structure only the dose U. baileyi 10,500 mg/kg Bw did not show a significantdifference with negative cotrol. It can be concluded that the dose of U. baileyi 10,500 mg/kg Bw rats in boiled sapgave the best effect in reducing damage to liver histology of rats induced by lead acetate. Key words: lead acetate, liver, sap, Usnea baileyi,

Subchronic Toxicity of Methanol Extract From Erythrina Variegata (Leguminosae) Leaves on Male Wistar Rats (Rattus Norvegicus) Tati Herlina; Madihah Madihah; Deden Deni; Suseno Amien
Molekul Vol 12, No 1 (2017)
Publisher : Universitas Jenderal Soedirman

Erythrina variegata is a herbal plant commonly used in Indonesia traditional medicine for treatment malaria disease. Acute toxicity test results showed that the methanol extract of E. variegata leaves categorized as practically nontoxic. In this study, we investigated sub chronic toxicity of the extract on male Wistar rats and the procedure based on guidelines of OECD 408 (1998) and EPA OPPTS 870.3100 (1998) for 90 constitutive days. The rats are classified into four dose groups were 0 (control), 250, 500, and 1000 mg/kg body weight with each group consist of five test animals. The results do not show toxic signs either behavior or body weight changed. Hematological analysis and clinical blood chemistry revealed slightly changes but were within the normal limits, except for BUN and SGPT values. Histopathological examination showed an increased damage of liver and kidney cells in form hydropic degeneration, fatty degeneration, as well as necrosis along with increased of extract doses that significantly different with control (p<0.05). However, the damage is reversible and was assumed had not has relations with the treatment. Thus, orally administration of E. variegata extract by dosage 250, 500, and 1000 mg/kg BW did not cause subchronic toxicity on male Wistar rats.

INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Ekayanti Mulyawati Kaiin; Muhammad Gunawan; Madihah Madihah; Ghina Nafisah
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.14±12.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 100±0.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.

Ethnobotanical Study of Medicinal Plants in Karangwangi, District of Cianjur, West Java Desak Made Malini; Madihah Madihah; Joko Kusmoro; Fitri Kamilawati; Johan Iskandar
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.5756

The knowledge and usage of plant as medicinal remedy by current generation are not as extensive as previous; therefore, many rural communities with restricted modern medical access still rely on traditional medicine. This paper provides significant ethnobotanical information on medicinal plants in Karangwangi Village of Cianjur District, West Java Indonesia. This study aimed to identify plants collected for medical purposes by the local people as well as to document the local names, uses, preparation, and location of these plants. Ethno botanical data was recorded by opting people participation and key informant approach involving semi-structured interviews, group discussions and filling of questionnaires. The results showed a total of 114 medicinal plants belonging to 50 families were identified. Zingiberaceae was the most-frequently cited (nine species), followed by Asteraceae, Euphorbiaceae, and Fabaceae (seven species each). The most-used plant parts were leaves (51.8%), followed by stems (22.9%) and the most common preparations were decoction, poultice and squeezed. Most of the plants were obtained from the house-yard and total of 30 medicinal uses were recorded. The ethnobotanical result documented in this study showed that this area is rich in medicinal plants and these plants are still commonly used for medicinal purposes among the people in their daily lives. Ethnobotanical heritage should be preserved, however, there is a gradual loss of traditional knowledge about these plants in new generation. Further, the findings can be used as baseline information for further scientific investigation for analyzing phytochemical, pharmaceutical and other biological activities for future drug discovery.

OPTIMALISASI DOSIS HORMON OVASPEC™ SEBAGAI INDUSER UNTUK PEMIJAHAN BUATAN IKAN NILEM BETINA GALUR PADJADJARAN Madihah Madihah; Anisa Muthia Fakhira; Fasya Nadhifa; Vanya Asdiqaputri Nugraha
Media Akuakultur Vol 18, No 1 (2023): Juni, 2023
Publisher : Pusat Riset Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.18.1.2023.1-7

Ikan nilem merupakan salah satu ikan air tawar asli di Indonesia dan berpotensi untuk dikembangkan dalam industri akuakultur. Dalam penelitian ini digunakan ikan nilem galur Padjadjaran yang dibudidayakan di Kawasan Perikanan Darat Ciparanje, Unpad. Penelitian ini bertujuan untuk mendapatkan dosis hormon Ovaspec™ yang optimal sebagai induser dalam pemijahan buatan ikan nilem betina melalui pengamatan peningkatan performa reproduksinya. Induk betina disuntik Ovaspec™ dosis 0, 0,3, 0,5, atau 0,7 ml/kg bobot badan (BB) secara intramuskular, dilanjutkan dengan pengalinan (stripping) untuk mengeluarkan telur dari ikan betina dan sperma dari ikan jantan. Sebagian telur dan sperma kemudian dicampurkan secara in vitro. Performa reproduksi untuk setiap kelompok perlakuan diamati pada lima ekor induk betina sebagai ulangan. Hasil penelitian menunjukkan bahwa perlakuan Ovaspec™ memengaruhi waktu laten pemijahan, kematangan telur, diameter telur, persentase fertilisasi, penetasan dan kesintasan larva hingga umur 3 hari pascatetas, namun tidak memengaruhi indeks gonadosomatik dan fekunditas absolut. Penggunakan Ovaspec™ dosis 0,7 ml/kg BB menghasilkan waktu laten paling singkat dan tingkat kematangan telur tertinggi, sedangkan dosis 0,5 ml/kg BB menghasilkan persentase penetasan dan kesintasan larva tertinggi. Disimpulkan bahwa dosis Ovaspec™ 0,5 dan 0,7 ml/kg BB dapat digunakan sebagai induser untuk pemijahan buatan yang juga meningkatkan kinerja reproduksi ikan nilem, khususnya galur Padjadjaran.Bonylip barb is one of the native freshwater fish in Indonesia and has the potential to be developed in the aquaculture industry. In this study, the Padjadjaran strain was cultivated in the Ciparanje inland fishery area, Unpad. This study aimed to obtain an optimal dose of Ovaspec™ hormone to aid the artificial spawning of female bonylip barbs based on the improvement of their reproductive performance. The female broodstocks were injected intramuscularly with Ovaspec™ doses of 0, 0.3, 0.5, or 0.7 ml/kg body weight (BW), followed by stripping to collect eggs from the female and milt from the male fish, which then proceeded to in vitro fertilization. Reproductive performance for each treatment group was observed in five females as a replication. The results showed that Ovaspec™ treatment affected the latency time of spawning, egg maturity level, egg diameter, fertilization rate, hatching rate, and postlarval survival rate at 3 days post-hatching but did not affect the gonadosomatic index (GSI) and absolute fecundity. Ovaspec™ dose of 0.7 ml/kg BW resulted in the shortest latent time and the highest egg maturity stage, while doses of 0.5 ml/kg BW resulted in the highest hatching rate and larval survival rate. It was concluded that Ovaspec™ doses of 0.5 to 0.7 ml/kg BW can be used as an inducer for artificial spawning and improve the reproductive performance of bonylip barb fish, particularly the Padjadjaran strain.

BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Mia Miranti; Hikmat Kasmara; Nurullia Fitriani; Melanie Melanie; Inas Qurrata A'yun; Yolani Syaputri; Febri Doni; Madihah Madihah; Sri Rejeki Rahayuningsih; Nabilah Sekar Azizah; Wawan Hermawan
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation. The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product.

Arab Descendant Identity in Media: A Discourse Analysis of Suara al-Irsyad Madihah, Madihah; Sarbini, Ahmad; Muhyiddin, Asep; Wibisono, M. Yusuf
Hanifiya: Jurnal Studi Agama-Agama Vol. 8 No. 1 (2025): Hanifiya: Jurnal Studi Agama-Agama
Publisher : Program Studi Studi Agama-Agama Pascasarjana UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/hanifiya.v8i1.44702

This study examines the representation of Arab descendants’ identity in Indonesia through the community-based media Suara Al-Irsyad (MSA). Media play a crucial role in shaping and preserving community identity, particularly within diasporic groups facing globalization and social transformation. This research aims to explore how MSA contributes to maintaining Arab descendants’ cultural identity and the strategies employed to remain relevant in the digital era. Employing a qualitative approach with a descriptive-analytical method, data were collected through direct observation, in-depth interviews with MSA administrators, and document analysis of its publications. Data analysis followed the Miles and Huberman model, encompassing data reduction, data display, and conclusion drawing. The findings indicate that MSA functions not only as an information platform but also as a medium for cultural preservation. Beyond reporting news and Islamic education, MSA constructs an inclusive narrative that highlights the contributions of Arab descendants to national development. To address digital challenges, MSA has implemented modernization strategies by leveraging social media platforms to engage younger audiences. Despite declining interest in community-based media and increasing competition from mainstream platforms, MSA sustains its relevance by balancing traditional values with technological innovation. This research contributes to media and identity studies by demonstrating the critical role of community media in constructing minority group representations in the public sphere. It enriches the discourse on diaspora identity dynamics in the context of media, particularly in navigating globalization and social change. Moreover, the study provides practical implications for media practitioners, scholars, and policymakers in formulating sustainable strategies for ethnic-based media in the digital era.

Learning Through "ARBOR Exhibition": Utilizing the Arboretum of Universitas Padjadjaran for Innovative Learning in Higher Education Rohmatullayaly, Eneng Nunuz; Irawan, Budi; Madihah, Madihah
Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Vol 13 No 1 (2025): June
Publisher : Program Studi Ilmu Perpustakaan UIN Alauddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/v13i1a15

The Arboretum Universitas Padjadjaran, as a living collection or museum, is a valuable educational resource for science education, promoting hands-on activities, student-centered learning, and rich social interaction. Aligned with the Outcome-Based Education (OBE) curriculum, the arboretum can be integrated to develop learning innovation, particularly in the “Digitalization of Biological Objects,” which leads to bio-curators’ competencies. Visual arts can be an excellent strategy for learning science, as it combines systematic work with creative thinking. Through this approach, the students can apply theory to real-life experiences. This study aimed to utilize the Arboretum Universitas Padjadjaran to implement innovative strategies for effective learning in higher education through exhibitions. This study used the Research and Development (R&D) method. “ARBOR: The Art and Beauty of Universitas Padjadjaran’s Pollinator” is a photography talk show and exhibition providing information about pollinators, food plants, and host plants in the arboretum and surrounding campus. A total of 135 curated photographs were exhibited digitally. During the exhibition, the students guided over 250 visitors on pollinators and their role in the ecosystem. Our result shows that project-based learning, such as “ARBOR” exhibitions, increased awareness regarding biodiversity and environmental issues and enhanced the students' leadership, communication, creative thinking, innovation, and collaboration skills, demonstrating the inspiring potential projects in higher education.

PERILAKU MAKAN IKAN NILA (Oreochromis niloticus L.) DI KOLAM CIPARANJE UNIVERSITAS PADJADJARAN KAMPUS JATINANGOR Angelica, Irene Teresa; Madihah, Madihah
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 1 (2025): BIOTIKA JUNI 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i1.63387

Ikan nila (Oreochromis niloticus L.) adalah ikan air tawar dengan prospek ekonomi tinggi. Analisis perilaku makan ikan dapat memberikan informasi mengenai komposisi pakan alami ikan yang bermanfaat untuk pengembangan protokol budidaya dan pelestarian populasinya. Perilaku makan ikan berkaitan dengan ketersedian jenis pakan di habitatnya. Penelitian ini bertujuan mengetahui perilaku makan ikan nila dari Kolam Ciparanje, Universitas Padjadjaran, Jatinangor berdasarkan hasil analisis frekuensi kejadian makanan dan rasio panjang relatif usus. Frekuensi kejadian makanan menyatakan jenis pakan dan kuantitasnya, rasio panjang relatif usus menyatakan golongan perilaku makan ikan berdasarkan anatomi usus. Metode penelitian ini adalah survei pada 15 ekor ikan nila berukuran 12,6-23,9 cm. Frekuensi kejadian makanan diamati dengan membandingkan jumlah lambung yang berisi suatu jenis pakan dengan total jumlah lambung yang diamati. Rasio panjang relatif usus ikan diukur dengan membandingkan rata-rata panjang total saluran pencernaan dengan panjang total tubuh ikan. Data dianalisis secara kuantitatif dan diinterpretasikan secara deskriptif. Hasil penelitian menunjukkan 21 jenis fitoplankton dan 1 jenis zooplankton pada lambung ikan nila. Fitoplankton yang mendominasi adalah Schroederia setigera pada 14 dari 15 lambung (93.33%), sementara zooplankton yang ditemukan hanya Filinia sp. pada 6 dari 15 lambung (40%). Nilai rasio panjang relatif saluran pencernaan adalah 5.36±. Hasil ini memperkuat penelitian lainnya yang menyatakan kebiasaan makan ikan nila adalah herbivora dengan preferensi pemakan plankton.

INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Madihah, Madihah; Nafisah, Ghina
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10896

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.1412.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 1000.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.

Co-Authors A'yun, Inas Qurrata Ahmad Sarbini, Ahmad Amalia Shalihah Andi Perdana Angelica, Irene Teresa Anisa Muthia Fakhira Arief Boediono Asep Muhyiddin, Asep Azizah, Nabilah Sekar Budi Irawan Deden Deni Desak M. Malini Desak Made Malin Desak Made Malini Dita Aprila Khoirunnisa Ekayanti Mulyawati Kaiin Eneng Nunuz Rohmatullayaly Fasya Nadhifa Febri Doni Febri Doni Firda Mohammad Firdous Fitri Kamilawati Fitriani Alfina Furqon, Wildanul Ghina Nafisah Hartanti Kusumaningtyas Hikmat Kasmara Hikmat Kasmara Hikmat Kasmara Iin Supartinah Inas Qurrata A'yun Indra A. Rochman Indriani Sasmita Johan Iskandar Johan Iskandar Joko Kusmoro Joko Kusmoro Kaiin, Ekayanti Mulyawati Kamilawati, Fitri Kartiawati Alipin Kusumaningtyas, Hartanti Lisda Mawarni Sihotang M. Yusuf Wibisono, M. Yusuf Malini, Desak M. Melanie Melanie Melanie Melanie, Melanie Mia Miranti Mia Miranti Muhammad Gunawan Muhammad Gunawan Nabilah Sekar Azizah Nafisah, Ghina Nining Ratningsih Nurullia Fitriani Nurullia Fitriani Perdana, Andi Rani Maharani Reina Yulianti Reina Yulianti Yulianti Rochman, Indra A. Sony H. Sumarsono Sri Rejeki Rahayuningsih Sumarsono, Sony H. Supartinah, Iin Suseno Amien Syaputri, Yolani Tati Herlina Tri Mayanti Vanya Asdiqaputri Nugraha Vita Novianti Wawan Hermawan Wawan Hermawan Wawan Hermawan Wildanul Furqon Yasmi Purnamasari Kuntana Yetti Yusri Gani Yolani Syaputri

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