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All Journal Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Indonesian Journal of Pharmaceutical Science and Technology Pro-Life Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Media Akuakultur Molekul: Jurnal Ilmiah Kimia Hanifiya: Jurnal Studi Agama-Agama Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Madihah Madihah
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Veterinary

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BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Miranti, Mia; Kasmara, Hikmat; Fitriani, Nurullia; Melanie, Melanie; A'yun, Inas Qurrata; Syaputri, Yolani; Doni, Febri; Madihah, Madihah; Rahayuningsih, Sri Rejeki; Azizah, Nabilah Sekar; Hermawan, Wawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2947

Abstract

The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation.  The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of  contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product.
Treatment of PLGA Nanoparticles Ointment-Ethanol Extract of Archidendron pauciflorum in the Wound Healing in Diabetic Mice Malini, Desak Made; Kuntana, Yasmi Purnamasari; Madihah, Madihah; Furqon, Wildanul; Hermawan, Wawan
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9256

Abstract

Diabetic wounds lead to severe tissue damage and are diffi-cult to cure. Jengkol (Archidendron pauciflorum) is a plant commonly used by local Indonesian communities to treat diabetic wounds. The efficiency of herbal medicine still has a deficiency of its ability to reach the target organs, therefore nanotechnology is applied in the hope that all drug concentrations can reach the target organs successfully. This study aimed to evaluate the effectiveness of PLGA nanoparticle ointment-ethanol extract jengkol fruit peel (EEJFP) to accelerate the wound healing process in the skin of streptozotocin-induced diabetic mice. The research method used was experimental with a completely randomized design using six treatments and four replications. Diabe-tes was induced by intraperitoneal injection of streptozotocin 180 mg/ kg BW. Mice with a blood glucose level of ≥150 mg/dL were used for diabetic mice models. The incision wound created at the dorsolateral region of shaven skin at ±1 cm2 using sterile scissors. The treatments given were vaseline for Control Negative (CN) and Control Positive (CP), Betadine ointment (PB), 10% EEJFP ointment (P1), 5% PLGA nanoparticle ointment-EEJFP (P2), and 2.5% PLGA nanoparticle ointment-EEJFP (P3). The results showed that the administration of PLGA nanoparticles ointment-EEJFP with a concentration of 5% PLGA nanoparticle ointment-EEJFP (P2) resulted in the shortest wound length on day 3, 7 and 14; narrower granulation tissue; a larger number of blood capillaries; and denser collagen fibers (α <0.05) compared to CP and PB treatments. The administration of PLGA nanoparticle ointment-EEJFP with a concentration of 5% was the most effective concentration in accelerating wound healing in the skin of diabetic mice. 
Co-Authors A'yun, Inas Qurrata Ahmad Sarbini, Ahmad Amalia Shalihah Andi Perdana Angelica, Irene Teresa Anisa Muthia Fakhira Arief Boediono Asep Muhyiddin, Asep Azizah, Nabilah Sekar Budi Irawan Deden Deni Desak M. Malini Desak Made Malin Desak Made Malini Dita Aprila Khoirunnisa Ekayanti Mulyawati Kaiin Eneng Nunuz Rohmatullayaly Fasya Nadhifa Febri Doni Febri Doni Firda Mohammad Firdous Fitri Kamilawati Fitriani Alfina Furqon, Wildanul Ghina Nafisah Hartanti Kusumaningtyas Hikmat Kasmara Hikmat Kasmara Hikmat Kasmara Iin Supartinah Inas Qurrata A'yun Indra A. Rochman Indriani Sasmita Johan Iskandar Johan Iskandar Joko Kusmoro Joko Kusmoro Kaiin, Ekayanti Mulyawati Kamilawati, Fitri Kartiawati Alipin Kusumaningtyas, Hartanti Lisda Mawarni Sihotang M. Yusuf Wibisono, M. Yusuf Malini, Desak M. Melanie Melanie Melanie Melanie, Melanie Mia Miranti Mia Miranti Muhammad Gunawan Muhammad Gunawan Nabilah Sekar Azizah Nafisah, Ghina Nining Ratningsih Nurullia Fitriani Nurullia Fitriani Perdana, Andi Rani Maharani Reina Yulianti Reina Yulianti Yulianti Rochman, Indra A. Sony H. Sumarsono Sri Rejeki Rahayuningsih Sumarsono, Sony H. Supartinah, Iin Suseno Amien Syaputri, Yolani Tati Herlina Tri Mayanti Vanya Asdiqaputri Nugraha Vita Novianti Wawan Hermawan Wawan Hermawan Wawan Hermawan Wildanul Furqon Yasmi Purnamasari Kuntana Yetti Yusri Gani Yolani Syaputri