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Sintasan dan pertumbuhan cacing Polychaeta Nereis sp. dari kawasan pertambakan Desa Jeruklegi Cilacap dengan salinitas media pemeliharaan dan jenis pakan berbeda Eko Setio Wibowo; Endah Sri Palupi; I G A Ayu Ratna Puspitasari; Atang Atang
Depik Vol 8, No 2 (2019): August 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.8.2.12155

Abstract. Polychaeta Nereis sp. is one of the natural foods that can trigger the maturation of shrimp gamete cells up to 70%, but the fulfillment of Nereis sp. still rely on the arrest from nature. This condition encourages the cultivation of Nereis sp., but knowledge of the biological aspects of Nereis sp. still very lacking, so it needs further research. This study aims to determine the survival and growth of Nereis sp. from Cilacap Jeruklegi area with different salinity and feed as basic information for the cultivation of Nereis sp. sustainable. The study was conducted experimentally with a randomized block design (RBD) method with six treatments including: S5PN (maintenance of Nereis sp. with 5 ppt salinity and feed with the main content of vegetable protein), S5PH (maintenance with salinity of 5 ppt and feed with the main content of animal protein), S15PN (maintenance of Nereis sp. With 15 ppt salinity and feed with the main content of vegetable protein), S15PH (maintenance with 15 ppt salinity and feed with the main content of animal protein), S25PN (maintenance of Nereis sp. With 25 ppt salinity and feed with main content of vegetable protein), S25PH (maintenance with 25 ppt salinity and feed with the main content of animal protein). The results showed that optimum salinity to support the survival of Nereis sp. It ranges from 5-15 ppt with animal and vegetable protein feed types. Nereis sp. Optimum growth. in the maintenance of 15 ppt salinity with animal protein feed types.Keywords: Nereis sp., Body weight, number of segments, Salinity, Feed Abstrak. Polychaeta Nereis sp. merupakan salah satu pakan alami yang mampu memicu pematangan sel gamet udang sampai 70%, tetapi pemenuhan kebutuhan Nereis sp. masih mengandalkan penangkapan dari alam. Kondisi ini mendorong adanya usaha budidaya Nereis sp., namun pengetahuan tentang aspek biologi Nereis sp. masih sangat kurang sehingga perlu penelitian lebih lanjut. Penelitian ini bertujuan mengetahui sintasan dan pertumbuhan Nereis sp. dari kawasan Jeruklegi Cilacap dengan salinitas pemeliharaan dan pakan yang berbeda sebagai informasi dasar untuk usaha pembudidayaan Nereis sp. yang berkelanjutan. Penelitian dilakukan secara eksperimental dengan metode rancangan acak kelompok (RAK) dengan enam perlakuan meliputi: S5PN (pemeliharaan Nereis sp. dengan salinitas 5 ppt dan pakan dengan kandungan utama protein nabati), S5PH (pemeliharaan dengan salinitas 5 ppt dan pakan dengan kandungan utama protein hewani), S15PN (pemeliharaan Nereis sp. dengan salinitas 15 ppt dan pakan dengan kandungan utama protein nabati), S15PH (pemeliharaan dengan salinitas 15 ppt dan pakan dengan kandungan utama protein hewani), S25PN (pemeliharaan Nereis sp. dengan salinitas 25 ppt dan pakan dengan kandungan utama protein nabati), S25PH (pemeliharaan dengan dengan salinitas 25 ppt dan pakan dengan kandungan utama protein hewani). Hasil penelitian menunjukkan bahwa salinitas optimum untuk mendukung sintasan Nereis sp. Berkisar 5-15 ppt dengan jenis pakan berprotein hewani dan nabati. Pertumbuhan optimum Nereis sp. pada pemeliharaan salinitas 15 ppt dengan jenis pakan berprotein hewani.Kata Kunci: Nereis sp., berat tubuh, jumlah segmen, salinitas, pakan

Biological aspects of Diopatra sp. (Onuphidae, Polychaeta) collected from mangrove habitats of Jeruklegi, Cilacap Regency Eko Setio Wibowo; IGA Ayu Ratna Puspitasari; Atang Atang; Joko Pamungkas
Depik Vol 11, No 3 (2022): DECEMBER 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.3.25787

Diopatra species are members of the family Onuphidae (Polychaeta, Annelida) that are widely used as fishing bait. These tubicolous worms are common in particular mangrove areas of Cilacap. However, biological aspects of Diopatra sp. inhabiting the areas have not been studied. In the present work, we investigated several biological aspects of the worms, including the segment number, the body weight and the sex ratio, based on Diopatra samples collected from four mangrove habitats of Jeruklegi Village, Cilacap Regency, between June and July 2021. The results show that the average numbers of segments of Diopatra sp. obtained ranged from 195 to 216 segments, whereas the average body weights ranged from 2.03 to 3.12 g, and the average sex ratios ranged from 0.5 to 1.3. In general, the biological aspects of Diopatra sp. between sampling sites were relatively similar; this can be attributed to the similar environmental conditions at all sampling sites, including salinity (25–32 ppt), water temperature (30–32 oC), dissolved oxygen (6.4–8.8 ppm) and pH (7). While Diopatra worm farming is necessary so that local people do not rely on natural catch, a further taxonomic study is required to reveal the identity of this economically important local species.Keywords:Biological aspectDiopatraMangrove habitatPolychaetesTubicolous worms

Sintasan dan pertumbuhan cacing Polychaeta Nereis sp. dari kawasan pertambakan Desa Jeruklegi Cilacap dengan salinitas media pemeliharaan dan jenis pakan berbeda Eko Setio Wibowo; Endah Sri Palupi; I G A Ayu Ratna Puspitasari; Atang Atang
Depik Vol 8, No 2 (2019): August 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.8.2.12155

Abstract. Polychaeta Nereis sp. is one of the natural foods that can trigger the maturation of shrimp gamete cells up to 70%, but the fulfillment of Nereis sp. still rely on the arrest from nature. This condition encourages the cultivation of Nereis sp., but knowledge of the biological aspects of Nereis sp. still very lacking, so it needs further research. This study aims to determine the survival and growth of Nereis sp. from Cilacap Jeruklegi area with different salinity and feed as basic information for the cultivation of Nereis sp. sustainable. The study was conducted experimentally with a randomized block design (RBD) method with six treatments including: S5PN (maintenance of Nereis sp. with 5 ppt salinity and feed with the main content of vegetable protein), S5PH (maintenance with salinity of 5 ppt and feed with the main content of animal protein), S15PN (maintenance of Nereis sp. With 15 ppt salinity and feed with the main content of vegetable protein), S15PH (maintenance with 15 ppt salinity and feed with the main content of animal protein), S25PN (maintenance of Nereis sp. With 25 ppt salinity and feed with main content of vegetable protein), S25PH (maintenance with 25 ppt salinity and feed with the main content of animal protein). The results showed that optimum salinity to support the survival of Nereis sp. It ranges from 5-15 ppt with animal and vegetable protein feed types. Nereis sp. Optimum growth. in the maintenance of 15 ppt salinity with animal protein feed types.Keywords: Nereis sp., Body weight, number of segments, Salinity, Feed Abstrak. Polychaeta Nereis sp. merupakan salah satu pakan alami yang mampu memicu pematangan sel gamet udang sampai 70%, tetapi pemenuhan kebutuhan Nereis sp. masih mengandalkan penangkapan dari alam. Kondisi ini mendorong adanya usaha budidaya Nereis sp., namun pengetahuan tentang aspek biologi Nereis sp. masih sangat kurang sehingga perlu penelitian lebih lanjut. Penelitian ini bertujuan mengetahui sintasan dan pertumbuhan Nereis sp. dari kawasan Jeruklegi Cilacap dengan salinitas pemeliharaan dan pakan yang berbeda sebagai informasi dasar untuk usaha pembudidayaan Nereis sp. yang berkelanjutan. Penelitian dilakukan secara eksperimental dengan metode rancangan acak kelompok (RAK) dengan enam perlakuan meliputi: S5PN (pemeliharaan Nereis sp. dengan salinitas 5 ppt dan pakan dengan kandungan utama protein nabati), S5PH (pemeliharaan dengan salinitas 5 ppt dan pakan dengan kandungan utama protein hewani), S15PN (pemeliharaan Nereis sp. dengan salinitas 15 ppt dan pakan dengan kandungan utama protein nabati), S15PH (pemeliharaan dengan salinitas 15 ppt dan pakan dengan kandungan utama protein hewani), S25PN (pemeliharaan Nereis sp. dengan salinitas 25 ppt dan pakan dengan kandungan utama protein nabati), S25PH (pemeliharaan dengan dengan salinitas 25 ppt dan pakan dengan kandungan utama protein hewani). Hasil penelitian menunjukkan bahwa salinitas optimum untuk mendukung sintasan Nereis sp. Berkisar 5-15 ppt dengan jenis pakan berprotein hewani dan nabati. Pertumbuhan optimum Nereis sp. pada pemeliharaan salinitas 15 ppt dengan jenis pakan berprotein hewani.Kata Kunci: Nereis sp., berat tubuh, jumlah segmen, salinitas, pakan

Morphoanatomy and size of male Alabio ducks (Anas platyrhynchos) reproductive organs of starter period Setiyono, Eko; Wijinindyah, Ayutha; Atang, Atang; Wibowo, Eko Setio; Sugiharto, Sugiharto
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.41342

The testes are vital for spermatogenesis and steroid hormone production, thus serving as a critical biomarker for monitoring testicular function through organ development analysis. Until now, there has been no information on the morphoanatomical development of testicular organs in day-old Alabio ducks (DOD). This knowledge is crucial for optimizing reproductive strategies and manipulations. This study aimed to determine the growth and morphoanatomical development of testes in Alabio ducks during the starter period. A purposive sample of forty DOD male Alabio ducks was observed and measured weekly for eight weeks. Variables included testicular morphoanatomy, weight, liver weight, gonadal-somatic index (GSI), and hepatic-somatic index (HSI). Results indicate that bean-shaped, creamy-white testes are located in the abdominal cavity near the spine, attached dorsally and anterior to the kidneys. Testicular weight, length, reproductive tract weight and length, and GSI peaked at eight weeks (0.768 ± 0.06 g, 1.73 ± 0.20 cm, 0.79 ± 0.02 g, and 13.32 ± 0.65, respectively; p < 0.01). Testicular weight, liver weight, GSI, and HSI differed significantly weekly (p < 0.01). In conclusion, testicular weight in Alabio ducks increases steadily during the first eight weeks, with bean-shaped testes developing bilaterally in the abdominal cavity, attached dorsally and constrained by the mesorchial ligament.

Effect of Batik Wastewater Resulted from Biosorption Process to Blood Glucose Level on Carp (Cyprinus carpio) Oktaviani, Safira Dwi; Lestari, Sri; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1819

Chromium from the process of coloring batik has high toxicity to the organism. The biosorption method is the heavy metal adsorption process using inactive organisms to eliminate or decrease chromium in batik wastewater. In a polluted environment, fish can be in a stressful condition that requires energy to adapt to environmental changes; high energy requirements will stimulate an increase in blood glucose levels. Chromium exposure can increase the blood glucose level on carp (Cyprinus carpio). This study aims to determine the effect of batik wastewater resulted from the biosorption process exposure to the blood glucose levels. This study used 4 concentrations with 6 replications. Carp is placed in a mixed water tank with batik waste resulting from biosorption. The treatment of batik wastewater produced from the biosorption process concentration are of 0%v.v-1; 39,657%v.v-1; 26,438%v.v-1; and 13,219%v.v-1. Research shows that chromium was decreasing the results obtained at K0 are 68 mg/dL, at K1 is 132 mg/dL, K2 is 127 mg/dL, and at K3 is 116 mg/dL Batik wastewater resulted from biosorption process increasing the blood glucose level in fish and accelerates the rate of expenditure on fish The optimaly concentration of batik wastewater resulted from biosorption process are tried can Keyword : Batik wastewater, Biosorption, Blood glucose level, Carp

PENGARUH SUPLEMENTASI Chlorella vulgaris PADA PAKAN UNTUK MENINGKATKAN ALBUMIN DALAM SERUM IKAN NILEM (Osteochilus vittatus) Zakiah, Kiki Siti; Simanjuntak, Sorta Basar Ida; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2144

Nilem fish belongs to freshwater fish commodity that has been widely cultivation. Some factors influencing fish cultivation are good quality of feed, quantity, size, and shape. Quality feed works as a primary energy source as well as improves the digestive system of fish, with the expectation that the fish growth and health are optimal. Information concerning health status in cultivation Nilem has been used as a reference for improving cultivation methods and controlling disease attacks. The population and production of fish cultivation is experiencing serious problems due to infection from various types of pathogens. Therefore, a viable alternative is needed to prevent and overcome diseases by utilizing immunostimulant substances derived from synthetic substances that can increase the activity of non-specific immune defenses and stimulate antibiotic-forming organs in fish body. Immunostimulant is a substance that is capable of increasing the mechanism of non-specific immune response of fish one of them is by adding Chlorella vulgaris extract to the feed. This research objective is to determine the influence of Chlorella vulgaris supplementation on total blood albumin in nilem fish. This research was conducted by sampling method consisting of 5 treatments with further 4 replications. The treatments were feed supplementation of 0 g.kg-1, 2 g.kg-1, 3 g.kg-1, 4 g.kg-1, and 6 g.kg-1. Independent and dependent variables were used in this research; the independent variable was the dose of Chlorella vulgaris in the feed and the dependent variable was blood albumin concentration. The measuring of plasma albumin concentration was done using the Dyasis kit method with a spectrophotometer. The obtained data were then analyzed by ANOVA, followed by the Least Significant Difference (LSD) at the 95% confidence level if there is a real difference then followed by Tukey's test at the same confidence level. The results showed that the average value of serum albumin concentration of nilem fish from all treatments was between 4.2761 – 7.1826 g/dL. The normal albumin concentration was found in the treatment 4 g.kg-1 Chlorella vulgaris supplementation which was 4.77 g/dL. Meanwhile, the highest concentration was found from the supplementation of Chlorella vulgaris at 3 g.kg-1 feed which was 6.677 g/dL.

Aspek-aspek Reproduksi Cacing Diopatra neapolitana Di Cilacap Purwati, Ita; Rachmawati, Farida Nur; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3494

Diopatra neapolitana merupakan polychaeta yang memiliki tubuh dengan struktur tabung besar. D. neapolitanan dapat dimanfaatkan sebagai pakan udang karena memiliki kandungan nutrisi yang cukup tinggi. Penggunaan D. neapolitana sebagai pakan dilakukan dengan mengambil cacing langsung dari alam sehingga dapat mengganggu keberlangsungan populasi cacing D. neapolitana di alam. Kondisi ini memerlukan adanya usaha domestifikasi dan budidaya D. neapolitana, namun informasi tentang kondisi biologis dan reproduksi yang ada masih terbatas, terutama spesies yang ditemukan di Cilacap. Penelitian ini bertujuan untuk mengetahui aspek-aspek reproduksi D. neapolitana yang dapat mendukung domestifikasi dan budidaya cacing D. neapolitana. Hasil penelitian menunjukkan nilai rasio jenis kelamin yang seimbang yaitu 1:1. Ukuran tubuh cacing betina dan jantan tidak berbeda nyata. Hasil pengamatan pada cacing betina menunjukkan rata-rata jumlah segmen 131,96 ± 28,19 buah; rata-rata berat tubuh 2.65 ± 1,15 g dan cacing berada dalam fase mature dan submature dengan diameter telur antara 199-240 µm. Cacing jantan memiliki rata-rata jumlah segmen 136,97 ± 24,51 buah; rata-rata berat tubuh 2.18 ± 1,11 g dan bentuk sperma berupa tetrad spermatid. Hasil analisis hubungan berat tubuh dan tingkat maturasi D. neapolitana menunjukkan hasil korelasi negatif dengan r= 0,009, persamaan regresi linier y= 219.868 – 0,080x; dan nilai t hitung (-0,043) < t tabel (2,07961), dan asil analisis hubungan jumlah segmen dan tingkat maturasi D. neapolitana didapat nilai r = 0,101; r2 = 1%; persamaan regresi linier y = 224.247– 0,035x; dan t hitung (-0,463) < t tabel (2,07961). Hasil analisis regresi linier menunjukkan bahwa %. Berat tubuh tidak berkorelasi positif dengan tingkat maturasi cacing D. neapolitana. Peningkatan berat tubuh tidak selalu dikuti dengan peningkatan tingakat maturasi, dan peningkatan jumlah segmen tidak selalu diikuti dengan peningkatan tingkat maturasi cacing D. neapolitana.

Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami Safitri, Nuridha Audinia; Atang, Atang; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4606

Plastic in Indonesian daily life cannot be avoided anymore. Materials for the manufacture of plastic can cause free radicals to the body, thus affecting the immune system. These materials are including phthalates and bisphenol-A (BPA), which commonly called as plasticizers. Free radicals in the body can be inhibited with antioxidants. Antioxidants can be obtained from endogenous (inside the body) and exogenous (outside the body). The purpose of this study was to determine the damage level of liver histopathological structure as the result of plastic boiled water induction towards natural antioxidants feeding on male mice (Mus musculus). This study used an experimental method with a completely randomized design (CRD) consisting of six treatments and five replications for 21 days. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. The independent variable is natural antioxidants and the dependent variable is the liver histopathology of male mice. The qualitative parameters observed were the liver histopathological structure of male mice is in the state of inflammatory cell infiltration, degeneration, and necrosis. The research data is an overview of the liver histopathological structure of male mice. The obtained data were analyzed using descriptive qualitative analysis method. Oral induction of boiled-plastic water can affect the changes of liver histological structure. Natural antioxidants given in male mice can maintain the tissue structure as the result of boiled-plastic water induction. The results showed the damage level of male mice liver histopathological structure as reversible degeneration.

Diferensial Leukosit Beberapa Spesies Ikan Tangkapan Dari Familia Cyprinidae Di Sungai Banjaran, Kabupaten Banyumas Irwanti, Nabilla Gina; Susatyo, Priyo; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4703

Familia Cyprinidae dapat ditemukan di hampir seluruh sungai Indonesia termasuk Sungai Banjaran, Kabupaten Banyumas. Diferensial leukosit bertujuan mengetahui perbedaan persentase sel leukosit. Penelitian ini untuk mengetahui hubungan antara lokasi pengambilan sampel (hulu, tengah, dan hilir) dengan diferensial leukosit dan mengetahui gambaran apusan darah dari beberapa spesies ikan familia Cyprinidae. Pengambilan sampel menggunakan teknik purposive sampling pada tiga stasiun yang berbeda. Data yang diperoleh dianalisis dengan metode Analysis of Variance (ANOVA) dengan tingkat kesalahan 5% dan gambaran hasil pengamatan mikroskopis jenis-jenis leukosit. Hasil penelitian menunjukkan bahwa perbandingan persentase sel lekosit pada Ikan Nilem (Osteochilus vittatus), Ikan Brek (Barbonymus balleroides), dan Ikan Lunjar (Rasbora argyrotaenia) dengan lokasi Hulu, Tengah, Hilir Sungai Banjaran, Banyumas, berbeda secara signifikan (p<0,05). Persentase sel leukosit yang paling tinggi ada pada zona Hilir di Sungai Banjaran, Banyumas yaitu sel neutrofil dan sel monosit. Presentase sel neutrofil Ikan Nilem didapatkan sebesar 9,5%, Ikan Brek sebesar 8,5% dan Ikan Lunjar sebesar 9,4%. Presentase sel monosit pada Ikan Nilem sebesar 8,9%, Ikan Brek sebesar 8,7% dan Ikan Lunjar sebesar 8,5%. Sel neutrofil memiliki sitoplasma bergranula halus dan ditengahnya terdapat inti bersegmen. Sel eosinofil mempunyai sitoplasma berwarna lebih merah, inti yang terletak memanjang di tepi sel, memiliki granula besar. Sel limfosit memiliki sitoplasma berwarna biru gelap dan inti berbentuk bulat hingga oval. Sel monosit memiliki sitoplasma berwarna biru pucat dan bentuk inti bervariasi ada yang berbentuk seperti ginjal hingga yang berbentuk dua lobus besar.

Efek Ekstrak Etanol Ganoderma lucidum terhadap Kadar Ureum dan Kreatinin pada Tikus Model Inflamasi Terinduksi Completed Freunds's Adjuvant (CFA) Nahdatulia, Yasinta; Ratnaningtyas, Nuniek Ina; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 3 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.3.5875

Jamur Lingzhi (Ganoderma lucidum) mengandung komponen bioaktif seperti terpenoid, polisakarida, nukleotida, sterol, steroid, asam lemak dan protein/peptida yang memiliki potensi obat. Senyawa bioaktif G. Lucidum sebagai anti inflamasi berhubungan dengan supresi ekspresi NF-κB yang merupakan regulator ekspresi gen mediator inflamasi. Pelepasan mediator inflamasi yang tidak terkontrol berdampak negatif pada ginjal sehingga dapat menyebabkan disfungsi ginjal. Peningkatan kadar ureum dan kreatinin merupakan salah satu biomarker disfungsi ginjal. Penelitian ini bertujuan untuk mengetahui pengaruh dan dosis efektif ekstrak G. lucidum terhadap penurunan kadar ureum dan kreatinin darah tikus putih jantan (Rattus norvegicus) galur Wistar yang diinduksi Complete Freund's Adjuvant (CFA). Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Kelompok perlakuan (P1, P2, dan P3) diberi perlakuan ekstrak jamur G. lucidum dengan variasi dosis 250, 500, 750 mg.kg-1 BB dengan lima kali ulangan. Parameter yang diamati adalah kadar ureum dan kreatinin sebagai penanda fungsi ginjal. Data pengukuran kadar ureum dan kreatinin dianalisis dengan uji ANOVA, dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak etanol G. lucidum mampu menurunkan kadar kreatinin dan ureum tikus yang diinduksi inflamasi CFA. Ekstrak etanol G. lucidum dengan dosis 750 mg.kg-1 BB dapat menurunkan kadar ureum dan kreatinin pada tikus dengan rata-rata kadar kreatinin 0,75±0,32 mg.dL-1 dan urea sebesar 57,50±4,82 mg.dL-1 dengan penurunan kadar kreatinin dan ureum dibandingkan kontrol negatif sebesar 60,93% dan 20,16%. Ekstrak etanol G. lucidum dengan dosis 750 mg.kg-1 BB efektif dalam menurunkan kadar ureum dan kreatinin pada tikus yang model inflamasi yang diinduksi CFA

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