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All Journal HAYATI Journal of Biosciences Perspektif : Review Penelitian Tanaman Industri Indonesian Journal of Pharmaceutical Science and Technology Elkawnie Majalah Obat Tradisional Abditani : Jurnal Pengabdian Masyarakat Jurnal Ilmiah Farmasi Farmasyifa Perspektif, Review Penelitian Tanaman Industri Jurnal Abdimas Kartika Wijayakusuma Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Medical Sains : Jurnal Ilmiah Kefarmasian Riset Informasi Kesehatan AGRIC kawanad Jurnal Medika: Medika Kartika : Jurnal Ilmiah Farmasi Medical Sains : Jurnal Ilmiah Kefarmasian Pharmacon: Jurnal Farmasi Indonesia Jurnal Farmasi Galenika
Akhirul Kahfi Syam
Fakultas Farmasi Universitas Jenderal Achmad Yani
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : AGRIC

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THE GROWTH OF PIMPINELLA ALPINA HOST CALLUS AT VARIOUS TREATMENTS OF PLANT GROWTH REGULATOR CONCENTRATIONS OF NAA. 2,4 D AND ITS COMBINATION WITH BAP Fahrauk Faramayuda; Muhammad Irwan; Akhirul Kahfi Syam
Agric Vol. 34 No. 2 (2022)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2022.v34.i2.p171-182

Abstract

Purwaceng (Pimpinella alpina) is an herbal plant that has aphrodisiac, diuretic, and tonic properties. The imbalance between the growth and the exploitation of this plant makes it included in Appendix I (endangered), so its conservation will be crucial. One method of the conservation of this plant is tissue culture. This method is considered one way to induct the callus. The research objective is to find the effect of various treatments of plant growth regulator concentrations of NAA. 2,4 D and its combination with BAP on the growth of purwoceng callus. Concentration of plant growth regulator NAA (0.5 mg L-1, 1 mg L-1, 2 mg L-1), 2,4-D (0.4 mg L-1, 0.8 mg L-1, 1.2 mg L-1 ), a combination of NAA with BAP (0.5 mg L-1 NAA: 1 mg L-1 BAP, 1 mg L-1 NAA: 2 mg L-1 BAP, mg L-1 NAA: 3 mg L-1 BAP) and a combination of 2, 4- D with BAP (1 mg LL 2,4-D : 0.5 mg L BAP, 1 mg L-1 2,4-D : 1 mg L-1 BAP, 2 mg L-1 2,4-D : 1 mg L-1 BAP) into Murashige Skoog medium. Then purwaceng leaf explants were planted on the medium until the growth was seen. This research shows that the plant growth regulator concentration has a different influence on the growth of callus that is recognized by the day of callus induction, colour and callus texture. MS media with growth regulator 1.2 mg L-1 2,4-D can grow callus faster (17 days) with friable texture.
Co-Authors Adinda Fitriani Adinugraha, Irham M. Akbar, Tzazkia Febriyana Amedea, Esanda Zulfi Ami Soega Dwigantina Ananda Tunjung Pertiwi Anggita Esa Putri Fitrichia Anna Choirunnisa, Anna Aprilia, Trialisa Ari Sri Windyaswari Ari Sri Windyaswari Ari Sri Windyaswari Ari Sri Windyaswari, Ari Sri Azhar, Farah Salsabilla Saidah Dadan Suryasaputra Desti Hermawati Dewi, Renti Mutiara Dewilestari, Meyra Pratami Dwi Ayu Pratiwi Dwigantina, Ami Soega Elfahmi Elfahmi, Elfahmi Ema Mutya Esanda Zulfi Amedea Fahrauk Faramayuda Fahrauk Faramayuda, Fahrauk Faizal Hermanto Farah Salsabilla Saidah Azhar Faramayudha, Fahrauk Farhan Febrinella, Catellya Fitriani, Adinda Fitrichia, Anggita Esa Putri Florence Meliawaty Handayani, Dewi Ratih Hermawati, Desti Hestiary Ratih Ismail, Nursafira Khairunnisa Jaka Permana Julia Ratnawati Julia Ratnawati Julia Ratnawati Julia Ratnawati, Julia Juliastuti, Henny Khaerunnisa, Rahmadaniah Komar Ruslan Wirasutisna Lahagu, Helga Nitulo Berliana Limbong, Remare Halomoan Mardiana, Grace Selly Meyra Pratami Dewilestari Muhamad Insanu Muhammad Irwan Mutya, Ema Nandi Agustian Pertiwi, Ananda Tunjung Pratiwi, Dwi Ayu Purwoko, Agus Puspita Satriyani Permatasari Surasa Putri, Dhiffa Namira Alifia Rahaju, Asih Rakhmat, Iis Inayati Ramdhani, Amalia Kusuma Remare Halomoan Limbong Renata Ananda Marthasedana Renti Mutiara Dewi Sabilia, Syahra Asfi Sabirin, Indah Puti R. Septiani, Usi Septiani, Vina Sheila Nur Hasanah Sheila Nur Hasanah Sigid Pamungkas Wicaksono Soraya Riyanti Soraya Riyanti Soraya Riyanti Soraya Riyanti, Soraya Sri Restu Andriyani Suci Nar Vikasari, Suci Nar Sufyan Zainul Arifin Sukrasno Suntana, Mutiara Sukma Surasa, Puspita Satriyani Permatasari SURYANI Totik Sri Mariani Trialisa Aprilia Usi Septiani Vina Septiani Wulan Anggraeni Wulandari, Yesi Yeni Karlina Yeni Karlina Yeni Karlina, Yeni Yesi Wulandari Yuslianti, Euis Reni