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All Journal Jurnal Pendidikan Biologi Undiksha Wahana-Bio: Jurnal Biologi dan Pembelajarannya Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Konservasi Hayati BERNAS: Jurnal Pengabdian Kepada Masyarakat Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Medical Chemistry and Bioinformatics Maximus: Journal of Biological and Life Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Journal of Jamu
Khaerunissa Anbar Istiadi
Program Studi Biologi, Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia 35365
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Pendidikan Biologi Undiksha

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Perbedaan Profil Protein Plasma Darah Mencit Menggunakan SDS-PAGE Tanpa Penambahan dan dengan Penambahan 2-Mercaptoethanol Istiadi, Khaerunissa Anbar; Khairani, Iffa Afiqa; Andriani, Silvia
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 2 (2024)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i2.82139

Abstract

SDS-PAGE is a method used to analyze proteins based on differences in molecular weight. Sample preparation in SDS-PAGE with 2-mercaptoethanol will reduce disulfide bonds in proteins. The sulfide bond reduction process will cause differences in protein conformation and profile. The purpose of this study was to compare the pattern of blood plasma protein profiles of mice analyzed using SDS-PAGE, with the addition of 2-mercaptoethanol and without the addition of 2-mercaptoethanol in a mixture of sample buffer solutions. The results of SDS-PAGE showed that the blood plasma protein profile of mice without the addition and with the addition of 2-mercaptoethanol showed differences in the number of protein bands, the molecular weight of several proteins and the thickness of the protein bands. There were nine protein bands with a molecular weight above 52 kDa in the profile without the addition of 2-mercaptoethanol, while there were only five protein bands with a molecular weight above 52 kDa in the profile with the addition of 2-mercaptoethanol. The difference in protein profile patterns shows that mouse blood plasma contains proteins containing disulfide bonds that can be reduced by 2-mercaptoethanol.
Co-Authors Aji Hafidz Talaga Alawiyah, Alawiyah Alfauzia, Nadia Amelia, Tiara Andreas Dwi Nugroho Andriani, Silvia Aniesti, Frigia Rafilia Anisa, Hida Arliani Nur Ariyanto, Dika Ariyanto Astuti, Ayu Widia Desi Budiono Deviany Deviany, Deviany Dewi Chusniasih DINA ANGGRAINI Dini Meilinda Dwi Anita Suryandari Erma Suryanti Fajri Arif Wibawa Fatimatuzzahra, Izza Fatriani, Rizka Febrina, Yane Fita Feerzet Achmad Ferdinanda, Thierry Garbono, Andrian Gres Maretta, Gres Hadi Sunaryo Handayani, Catie Hariyandi, Yopi Hida Arliani Nur Anisa Iffa Afiqa Khairani Imaniar, Lisana Husna Irandi Putra Pratomo Jannah, Umi Nur Luluk Yunaini Muhyi, Abdul Mulyana, Jeane Siswitasari Nur Anisa, Hida Arliani Puji Sari Reni Yuniarti Restiawati, Nawang Sari, Dian Anggria Satria, Arysca Wisnu Siragih, Rajuman S Suryanti, Erma Triya Yuli Andini Yuni Sukmawati Yusuf, Reggina Aulia Zhofiroh, Nawrah Meisyah Muthi'ah