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All Journal e-Journal Pustaka Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Jurnal Kesehatan Gigi Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Health Notions Meditory : The Journal of Medical Laboratory SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Medica Hospitalia Jurnal Kesehatan Denta: Jurnal Kedokteran Gigi Odonto dental journal Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember Dental Agromedis ABDIGI: Jurnal Pengabdian Masyarakat Kesehatan Gigi
Agustin Wulan Suci Dharmayanti
Bagian Biomedik, Fakultas Kedokteran Gigi, Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Uji aktivitas antibakteri dan cemaran mikroba daun biduri (Calotropis gigantea l.) sesuai standar mutu bahan baku obat herbal: studi eksperimental laboratoris Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Dharmayanti, Agustin Wulan Suci; Ratna Dewanti, I Dewa Ayu; Yunianti, Ervisya Nandya
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.58528

Abstract

Pendahuluan. Daun biduri (Calotropis gigantea) digunakan oleh masyarakat sebagai obat tradisional khususnya mengobati sakit gigi. Ekstrak daun biduri diketahui memiliki kandungan senyawa flavonoid, triterpene glycosides, abrin, dan alkaloids. Salah satu syarat untuk menjadikan tanaman obat sebagai sediaan farmasi diperlukan standarisasi cemaran mikroba dan uji antibakteri. Tujuan penelitian ini untuk menganalisis aktivitas antibakteri dan cemaran mikroba daun biduri. Metode: Uji cemaran mikroba menggunakan metode angka lempeng total bakteri dan kapang. Sedangkan uji aktivitas antibakteri menggunakan metode difusi cakram dengan konsentrasi ekstrak daun biduri 15%, 20%, 25% dan 30% terhadap bakteri Streptococcus mutans dan Porphyromonas gingivalis. Data  untuk uji aktivitas antibakteri dianalisis menggunakan Anova One Way sedangkan untuk uji cemaran mikroba menggunakan metode deskriptif kuantitatif dibandingkan dengan standar cemaran mikroba berdasarkan Peraturan Kepala Bidang Pengawas Obat dan Makanan (BPOM). Hasil: Uji antibakteri terhadap S. mutans menunjukkan hasil zona hambat kategori sedang, dengan rata-rata diameter zona hambat dari kontrol negatif (0 mm), konsentrasi 15% (8,94 mm), 20% (9,05 mm), 25% (9,65 mm), 30% (9,79 mm), dan kontrol positif (22,43 mm). Sedangkan pada P. gingivalis tidak ada zona hambat. Hasil uji statistik didapatkan nilai signifikansi p=0,00 (p<0,05). Hasil uji cemaran mikroba menunjukkan hasil perhitungan jumlah bakteri 13.300 cfu/gram dan tidak ditemukan pertumbuhan kapang. Simpulan: Ekstrak daun biduri memiliki daya hambat terhadap Streptococcus mutans, tetapi tidak memiliki daya hambat terhadap Porphyromonas gingivalis. Cemaran ekstrak daun biduri masih memenuhi syarat batas cemaran sesuai standar bahan baku obat herbal.Antibacterial Activity Test and Microbial Contamination of Biduri Leaves (Calotropis gigantea L.) according to Quality Standards for Herbal Medicine: Experimental Laboratory StudiIntroduction. Biduri leaves (Calotropis gigantea) are used by the community as traditional medicine, especially to treat toothache. Biduri leaf extract is known to contain flavonoid, triterpene glycosides, abrin, and alkaloids. One of the requirements for making medicinal plants as pharmaceutical preparations requires standardization of microbial contamination and antibacterial test. Objective: to analyze the antibacterial activity and microbial contamination of biduri leaves. Method: The microbial contamination test uses the total bacterial and fungal plate count method. While the antibacterial activity test uses the disc diffusion method on 15%, 20%, 25% and 30% biduri leaf extract against Streptococcus mutans and Porphyromonas gingivalis. Data for antibacterial activity test were analyzed using Anova One Way while for microbial contamination test using quantitative descriptive method and compared with microbial contamination in accordance with Regulation of Head of Drug and Food Supervisory Division (BPOM). Results: Antibacterial test against S. mutans showed moderate inhibition zone result, with average diameter of inhibition zone from negative control (0 mm), concentration 15% (8,94 mm), 20% (9,05 mm), 25% (9,65 mm), 30% (9,79 mm), and positive control (22,43 mm). Meanwhile, in P. gingivalis there is no inhibition zone. Statistical test result obtained significance value p=0.00 (p<0.05). Microbial contamination test result showed bacterial count result 13,300 cfu/gram and no mold growth was found. Conclusion: Biduri leaf extract has inhibition against S. mutans, but has no inhibition against P. gingivalis. The contamination of Biduri leaf extract still meets the contamination limit according to the standard of herbal medicinal raw materials.
Uji efektivitas antibakteri daun Biduri (Calotropis gigantea) terhadap pertumbuhan Streptococcus mutans: studi eksperimental laboratoris Widodo, Alaya Dwi Salvahira; Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Suci Dharmayanti, Agustin Wulan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.56148

Abstract

Pendahuluan: Karies merupakan penyakit jaringan keras pada gigi yang disebabkan oleh asam hasil bakteri plak yang menumpuk pada permukaan gigi. Salah satu bakteri plak adalah Streptococcus mutans. Penggunaan obat kumur Chlorhexidine gluconate 0,2% terbukti mampu menghambat S.mutans. Namun penggunaan Chlorhexidine gluconate 0,2% dalam jangka panjang dapat menimbulkan efek samping. Oleh karena itu, diperlukan bahan alami seperti tanaman biduri yang memiliki efek antibakteri untuk menghindari efek samping tersebut. Penelitian ini bertujuan menganalisis ekstrak daun biduri (Calotropis gigantea) dapat menghambat pertumbuhan S.mutans. Metode: Jenis penelitian eksperimental laboratorium. Penelitian ini menggunakan 6 kelompok perlakuan yaitu kelompok perlakuan ekstrak daun biduri dengan konsentrasi 15%, 20%, 25%, 30%, K(-) akuades, dan K(+)Chlorhexidine gluconate 0,2%. Kelompok perlakuan dan kelompok kontrol dipaparkan S.mutans untuk melihat efek antibakterinya dan menggunakan metode spektrofotometri UV-Vis dan hitung koloni bakteri (total plate count). Hasil: Hasil penelitian menunjukkan, masing-masing konsentrasi menunjukkan hasil laju penghambatan sebesar 0 pada kelompok K(-), 45,78% pada kelompok 15%, 34,93% pada kelompok 20%, 76,5% pada kelompok 25%, 64,45% pada kelompok 30%, dan 89,45% pada kelompok K(+). Hasil  hitung koloni bakteri penghambatan bakteri terbesar yaitu pada konsentrasi 25%. Hasil yang diperoleh dilakukan analisis data. Data sebelum dan sesudah inkubasi dilakukan uji Paired T Test dan menunjukkan perbedaan yang signifikan. Analisis data laju penghambatan dilakukan dengan uji Kruskal Wallis dilanjutkan uji Mann Whitney Ekstrak daun biduri konsentrasi 15% dibandingkan dengan konsentrasi 20% dan 30% dibandingkan dengan 25% memiliki nilai p>0,05 sehingga tidak memiliki perbedaan yang bermakna. Simpulan: Daun biduri efektif menghambat pertumbuhan S.mutans.Antibacterial effectiveness test of biduri leaf (Calotropis gigantea) against Streptococcus mutans: experimental laboratory studyIntroduction: Caries is a disease that affects the hard tissue of the teeth, caused by acid deposits produced by plaque bacteria that accumulate on the tooth surface. One of the plaque bacteria is Streptococcus mutans. Chlorhexidine gluconate 0.2% mouthwash has been shown to inhibit S. mutans. However, prolonged use of Chlorhexidine gluconate 0.2% mouthwash can cause side effects. To avoid these side effects, natural ingredients like biduri plant that have antibacterial effects are needed. This research aims to analyze the inhibitory effects of biduri (Calotropis gigantea) leaf extract on the growth of S. mutans. Methods: This research is experimental laboratory study. Six treatment groups were used, four groups treated with biduri leaf extract at concentrations of 15%, 20%, 25%, 30%; the K(-) using distilled water,; and K(+) using Chlorhexidine gluconate 0.2%. The treatment and control group were exposed to S. mutans to evaluate its antibacterial effect and assessed using the UV-Vis spectrophotometric and total plate count method. Results: In the research conducted, each concentration showed varying inhibition rates. The inhibition rates observed were as follows: 0% for the K(-), 45,78% for the 15% group, 34,93% for the 20% group, 76,5% for the 25% group, 64,45% for the 30% group, and 89,45% for the K(+). In bacterial colony count, the greatest bacterial inhibition was observed at concentration of 25%. Statistical analysis was performed on the obtained data. A Paired T-Test demonstrated a significant difference between the data before and after incubation. Further analysis using the Kruskal Wallis and Mann Whitney tests revealed no significant difference in inhibition rates between 15% and 25% concentrations, or between 25% and 30%. Conclusion: Biduri leaf extract effectively inhibits the growth of S. mutans. 
Examining the Impact of Porphyromonas gingivalis Infection on Ovarian Health: A Reduction in Primordial Follicles in a Female Periodontitis Model Meilawaty, Zahara; Galau, Argha Sirna; Permatasari, Sabania Dinar; Setyaningsih, Sari; Astuti, Pudji; Setiawatie, Ernie Maduratna; Hendarto, Hendy; Suci Dharmayanti, Agustin Wulan
Jurnal Kesehatan Gigi Vol 12, No 1 (2025): Juni 2025
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v12i1.12547

Abstract

Periodontitis is a chronic inflammation of the periodontal tissues caused by periodontal pathogens, such as Porphyromonas gingivalis (P. gingivalis). P. gingivalis possesses several virulence factors that enable it to invade and spread through the bloodstream to other organs, including the ovaries. It triggers inflammation, which may impact the number of primordial follicles. The aim of this study was to assess the effects of P. gingivalis infection on the number of primordial follicles in the ovaries. This study was a laboratory experiment with a post-test control group design. The rats were divided into control and periodontitis groups. The periodontitis group consisted of rats induced with P. gingivalis in their sulcus gingiva of the first mandible molar every three days for 19 days. Therefore, control groups were without P. gingivalis induction. Seven days after the last induction of P. gingivalis, the rats were euthanized, and their ovarian tissues were collected for histological preparation. The primordial follicles were counted under 400x magnification. The results indicated that the treatment group had a significantly lower number of primordial follicles (2.2 ± 2.61) compared to the control group (12.4 ± 2.41). The study concludes that P. gingivalis infection led to a reduction in the number of ovarian primordial follicles in periodontitis models.
The effect of cassava (Manihot esculenta) leaf extract on COX-2 expression in the neutrophil cell culture exposed to the lipopolysaccharide of Escherichia coli (in-vitro study) Meilawaty, Zahara; Dharmayanti, Agustin Wulan Suci; Prafitasari, Dinar
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.311 KB) | DOI: 10.24198/pjd.vol31no1.16950

Abstract

Introduction: Neutrophils are cells which played an initial role in the inflammation area and can be induced by lipopolysaccharide (LPS). Activated neutrophils will secrete the COX-2 enzyme which is involved in arachidonic acid synthesis to produce inflammatory mediators. However, excessive inflammation can cause tissue destruction; thus, the body needs anti-inflammation agents. Cassava leaf contains biochemical component which has an anti-inflammation effect. This study was aimed to determine the expression of COX-2 in neutrophils administered with cassava leaf extract and the LPS of E. coli. Methods: This study was experimental laboratories (in vitro study) using human neutrophil cells culture. The samples of this study were divided into 4 groups, administered with 12.5% cassava leaf extract (T1); 25% cassava leaf extract (T2); control group which contained only neutrophils cells (C1); and control group contained neutrophils and LPS (C2). Human neutrophil cells culture was incubated in the 12.5% and 25% cassava leaf extract before exposed with the LPS of E. coli. COX-2 expression was observed with immunochemistry methods. Results: The COX-2 expression in the 12.5% cassava leaf extract group was significantly higher than the 25% cassava leaf extract group (p < 0.05). Conclusion: Cassava leaf extract can inhibit the COX-2 expression and can be used as an alternative anti-inflammation agent.Keywords: Anti-inflammation, cassava leaf extract, COX-2 expression, neutrophils.
The potential of cassava leaves extract in gingival fibroblasts of periodontitis of rats with ovarian dysfunction Shita, Amandia Dewi Permana; Meilawaty, Zahara; Rothschildi, Dhesyarmani Putri; Dharmayanti, Agustin Wulan Suci; Hamzah, Zahreni
Padjadjaran Journal of Dentistry Vol 33, No 3 (2021): November
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no3.29611

Abstract

Introduction: The prevalence of periodontitis has reached 42.8% in Indonesia. One contributing factor is Porphyromonas gingivalis. The ovarian dysfunction caused by ovariectomy procedures may also cause periodontitis. Post ovariectomy conditions resemble menopause, lowering the production of estrogen and progesterone hormones, leaving patients vulnerable to periodontitis. To prevent the side effects of the treatment, herbal ingredients are commonly used. The extract of cassava leaves is known to have pharmacological effects as an antioxidant and, thus, can be applied as a medicine for periodontitis.      Methods: A total of 21 rats were divided into three groups, (1) control: healthy rats; (2) Group P.1: P. gingivalis-induced rats; (3) Group P.2: ovariectomized rats. Groups P.1 and P.2 were further divided into three subgroups that were administered cassava leaves extract at a dosage of 179.2 mg/ kg BW, aquades and vitamin C. Rats were euthanized at day eight after the initial treatment to collect left mandibular first molar. The mandibular sections were immunohistochemically stained to quantify SOD expression using light microscope while the Image J software was used. Fibroblast cells expressing SOD are characterized by brown coloration in the cytoplasm and darker nucleus. Results: In periodontitis conditions, either due to P.gingivalis induction or ovarian dysfunction, fibroblast cells in rats given cassava leaves extract expressed higher SOD than rats given aquades, but still lower than rats given vitamin C. The result of the one way ANOVA test showed p value of 0,00, which means that there is a significant difference in all groups. Conclusion: Cassava leaves extract demonstrated the potential to increase the antioxidant expression in P. gingivalis-induced and ovarian dysfunction rats.
Correlation between general health, nutrition, and oral health status of elderly in a typical retired community-house Kusumawardani, Banun; Dharmayanti, Agustin Wulan Suci; Robin, Dwi Merry Christmarini; Rahayu, Yani Corvianindya; Fitriyana, Nurul Isnaini
Padjadjaran Journal of Dentistry Vol 33, No 2 (2021): July 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no2.18950

Abstract

Introduction: Indonesia is in an aging population era marked by an increase in elderly population above 7%. An enhancement of elderly population presents health problems due to organ function deterioration and requires extraordinary attention. General health, nutrition, and oral health status are the most problems claimed by elderly, particularly retired. Objective of this study was to analyze the correlation between the general health, nutrition, and oral health status of elderly. Methods: Analytical descriptive research with cross sectional method was carried out on 36 respondents in Paguyuban Purnabakti, Patrang, Jember, East Java Province. The sampling was done by purposive sampling. Information about social-demography and general health status of respondents were obtained from the questionnaire. The social-demography investigation comprised sex, age, education level and status of residence. While general health observation, it comprised smoking status, general check-up, presence of systemic diseases, blood pressure and blood biochemical parameters.[CS1]  Nutritional status was examined by body mass index (BMI). Oral examination for identifying oral health status included the number of the teeth remaining in the oral cavity, use of dentures, oral hygiene index (OHI-s), and periodontal tissue health (CPITN). Results: The result exhibited that most of the respondents presented good general health status. However, there was no significant correlation between routine general health observation and age (p=0.906). Moreover, there was no significant relationship between blood biochemical profile (blood glucose, total cholesterol, and blood pressure level) with nutrition status (p=..., p=..., and p=... respectively), and oral health status (p=...), except with oral hygiene (p=...). Conclusion: This study is limited to Paguyuban Purnabakti, Patrang, Jember, East Java Province. Although this study could not be generated in all communities, this study concluded no relationship between general health (smoking, systemic disorders, and routine general health observation), nutritional status, and oral health in the elderly. [CS1]Disesuaikan dengan table 2
Edukasi Pemanfaatan Rimpang Kunyit untuk Meningkatkan Imunitas Tubuh di Pandemi COVID-19 Syafira Az Zahro; Agustin Wulan Suci Dharmayanti; Dwi Warna Aju Fatmawati; Lusi Hidayati
Dental Agromedis Vol. 2 No. 1 (2024): Mei
Publisher : Fakultas Kedokteran Gigi, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/da.v2i1.1463

Abstract

In 2019, a new type of corona virus (SARS-CoV-2) has spread, whose disease is called Coronavirus disease 2019 (COVID-19). This virus was discovered in Wuhan, China for the first time and has infected 90,308 people as of March 2, 2020. The number of deaths has reached 3,087 people or 6%, the number of patients who have recovered is 45,726 people. Common symptoms include fever, cough and difficulty breathing. Until the beginning of 2021, Covid 19 was still a pandemic that occurred in Indonesia. One year on, there are still many people who do not understand how to properly implement the COVID 19 protocol. Indonesia is among the top 25 countries in the world with the highest number of positive cases of coronavirus disease (Covid-19). East Java is one of the provinces in Indonesia which accounts for the most positive cases of corona. The number of confirmed positive patients in East Java continues to increase due to the lack of public awareness in implementing health protocols as an effort to prevent transmission of the coronavirus. In times of apandemic it is not known when it will end, anticipation such as strengthening immunity also needs to be done. The curcumin found in the turmeric plant can be used as a drink to increase immunity. Besides being easy to find, turmeric also has a cheap price. The identical taste of herbal medicine that some people don't like causes people to think that increasing body immunity in the midst of the COVID -19 pandemic will be less effective. So that a tea extract from turmeric is made that is easy for the community to practice on a daily basis. The method used is direct education. The education was carried out to help the community by distributing information about the COVID-19 health protocol and also increasing immunity to prevent exposure to COVID-19.Keywords: COVID-19; Curcumin; Housewife.
Pelatihan Pembuatan Video Animasi untuk Guru Paud Miftahul Ibad sebagai Media Edukasi Kesehatan Interaktif Dian Nurjannah; Agustin Wulan Suci Dharmayanti
Dental Agromedis Vol. 2 No. 1 (2024): Mei
Publisher : Fakultas Kedokteran Gigi, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/da.v2i1.1464

Abstract

The COVID-19 pandemic, declared to end in 2023 through Presidential Decree Number 17 of 2023, teaches us always to live clean and healthy. The rapid spread of the virus that causes COVID-19 is related to society's inadequacy in practising clean and healthy living. Early childhood children are unique because they prefer interactive learning systems, especially those that involve their audio-visual abilities. Their cognitive development tends to explore and learn through direct experience. Visuals full of images, colours and movement will attract the attention of young children and make it easier for them to describe abstract concepts that are difficult to understand, including how to live a clean and healthy lifestyle. The target audience for this service activity is the Miftahul Ibad Integrated Preschool teacher in Mojoparon Village. This basic cognitive ability is a strong basis for the community service team to teach PAUD teachers how to make animated videos to make it easier to convey material to young children. This service activity was carried out through several methods or stages, namely a) the the preparation and design stage, b) the the implementation stage, and c) the the monitoring and evaluation stage. These three stages were carried out at the Miftahul Ibad Integrated Early Childhood Foundation, Mojoparon Village, Rembang District, Pasuruan Regency, East Java. Community service activities at the Miftahul Ibad Integrated Preschool run smoothly. This activity was carried out in the teacher's room. Community service activities through training in making animated videos about living and healthy behaviour for PAUD teachers via laptop and cellphone devices can be followed well. Hopefully,g will be useful for students in improving clean and healthy behaviour.Keywords: audiovisual; Covid-19; early childhood; video making
Inovasi Pembuatan Handsanitizer dan Pencegahan Penyakit Menular Melalui Pemberdayaan Wanita Dharmayanti, Agustin Wulan Suci; Tsalis, Dina; Indriana, Tecky; Fatmawati, Dwi Warna Aju; Permatasari, Yulita Indah
Dental Agromedis Vol. 3 No. 1 (2025): Mei
Publisher : Fakultas Kedokteran Gigi, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/da.v3i1.5969

Abstract

In recent years, the Covid-19 pandemic has highlighted the importance of personal hygiene to prevent the spread of infectious diseases. This article examines the innovation of natural ingredient-based hand sanitizer production and the empowerment of women through training in Dusun Krajan, Lengkong Village, Mumbulsari District, Jember. By providing education and practical training in making hand sanitizer and moringa leaf tea, this initiative aims to enhance women's capacity to maintain family and community health. The training results indicate an increase in participants' knowledge and skills in producing safe sanitation and strengthening women's participation in public health. In conclusion, the empowerment of women through this initiative can enhance the well-being of families and communities as a whole.Keywords: battle leaves, hand sanitizer, infectious diseases, moringa tea, women empowerment
Ekstrak daun biduri (Calotropis gigantea) sebagai anti inflamasi dalam menurunkan jumlah neutrofil pada model tikus inflamasi: studi eksperimental Permata, Dea; Meilawaty, Zahara; Astuti, Pudji; Dharmayanti, Agustin Wulan Suci; Setyaningsih, Sari; Prasetya, Rendra Chriestedy
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 37, No 2 (2025): Agustus 2025
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v37i2.61210

Abstract

Pendahuluan: Inflamasi adalah respons imun terhadap rangsangan berbahaya yang bertujuan untuk menghilangkan kerusakan dan memulai penyembuhan. Proses inflamasi dimulai dengan pelepasan neutrofil yang mengatur dan memperkuat respon inflamasi namun. Obat Anti-Inflamasi Non-Steroid (NSAID), seperti aspirin, sering digunakan sebagai anti inflamasi tetapi memiliki efek samping. Ekstrak daun biduri (Calotropis gigantea)  yang kaya akan flavonoid, tanin, dan saponin, yang memiliki sifat anti-inflamasi dan penyembuhan luka dengan efek samping yang lebih sedikit dapat digunakan sebagai obat alami alternatif. Penelitian ini bertujuan untuk menganalisis perbedaan kemampuan ekstrak daun biduri (Calotropis gigantea) dalam menurunkan jumlah neutrofil pada inflamasi akut dan subakut pada model tikus inflamasi. Metode: Penelitian ini adalah studi eksperimental in vivo menggunakan desain serial group design. Daun biduri diekstrak menggunakan metode maserasi dengan pelarut etanol 70%. Dosis ekstrak daun biduri yang digunakan adalah 100 mg/kg BB, 200 mg/kg BB, dan 300 mg/kg BB. Tikus wistar diinjeksi dengan karagenan 2% pada punggungnya secara subkutan sebagai model inflamasi. Sampel darah diambil pada interval tertentu untuk membuat hapusan darah, selanjutnya dilakukan penghitungan jumlah neutrofil. Analisis menggunakan Two-way Anova yang dilanjutkan dengan uji LSD. Hasil: Ekstrak daun Calotropis gigantea pada dosis 100 mg/Kg BB, 200 mg/Kg BB, dan 300 mg/Kg BB terbukti dapat mengurangi jumlah neutrofil, terutama pada hari ke-3, dengan dosis 300 mg/Kg BB memberikan hasil terbaik (P=0,001). Simpulan: Pemberian ekstrak daun biduri (Calotropis gigantea) dosis 300 mg/Kg BB memberikan hasil terbaik dalam menurunkan jumlah sel neutrofil pada inflamasi akut dan subakut pada model tikus inflamasi.Calotropis gigantea leaf extract as an anti-inflammatory agent in reducing neutrophil count in an inflammatory rat model: an experimental studyIntroduction: Inflammation is an immune response to harmful stimuli that serves to eliminate tissue damage and initiate healing. The inflammatory process begins with the release of neutrophils, which regulate and amplify the inflammatory response. Non-steroidal anti-inflammatory drugs (NSAIDs), such as aspirin, are commonly used to treat inflammation but may cause side effects. Biduri leaf (Calotropis gigantea)extract which is rich in flavonoids, tannins, and saponins, possesses anti-inflammatory and wound-healing properties with fewer side effects, making it a potential natural alternative medicine. This study aims to evaluate the effectiveness of biduri leaf extract (Calotropis gigantea) in reducing the number of neutrophils during acute and subacute inflammation in an experimental rat model. Methods: This study is an in vivo experimental study using a serial group design. Biduri leaves were extracted using the maceration method with 70% ethanol as the solvent. The biduri leaf extract was administered at doses of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Wistar rats were injected subcutaneously with 2% carrageenan on the dorsal region to induce inflammation. Blood samples were collected at predetermined intervals to prepare blood smears, followed by neutrophil counting. Data were analyzed using two-way ANOVA followed by the LSD post-hoc test. Results: Calotropis gigantea leaf extract at doses of 100 mg/Kg BW, 200 mg/Kg BW, and 300 mg/Kg BW significantly reduced neutrophil counts, particularly on day 3, with the 300 mg/Kg BW dose showing the most pronounced effect (p=0.001). Conclusion: Administration of biduri leaf (Calotropis gigantea) extract at a dose of 300 mg/Kg BW demonstrated the highest effectiveness in reducing neutrophil count during acute and subacute inflammation in an inflammation rat model.
Co-Authors Adriyanti Ailsa Luthfie Ahmad Bagus Prayoga Amandia Dewi Permana Shita Amandia Dewi Permana Shita Amelia Nur Ilahi Anggun Kharisma Putri Anya Tania Larasati Arifin, Grandissyaikhu Kamila Asadi, Ahmad Ahya Bangun Febrianto Banun Kusumawardani Banun Kusumawardani Budi Yuwono Deasy Kusuma Ardiani Desy Putri Dwi Fitriana Dhea Ayu Dewanti Dian Nurjannah Dina Tsalis Wildana Dina Tsalis Wildana Dwi Aji Nugroho, Dwi Dwi Kartika Apriono Dwi Kartika Apriono, Dwi Kartika Dwi Merry Christmarini Robin Dwi Warna Aju Fatmawati Dwi Warna Aju Fatmawati Dwi Warna Aju Fatmawati Edwyn Saleh Eka Risky Fitria Fauziah Endah Aryati Eko Ningtyas Erfin Ramadana Pratama Erlina Sih Mahanani, Erlina Sih Ernie Maduratna Setiawatie Fajar Risqi Zaenulloh Farizan Zata Hadyan, Farizan FEBRIANTO, BANGUN Fina Khoirun Nisa’ Fiqih Haikal Fitriyana, Nurul Isnaini Galau, Argha Sirna Hendy Hendarto Hidayatur Rofi’ah I Dewa Ayu Susilawati Ita Kurniawati Kosasih, Jane Kusuma Ardiani, Deasy Kusuma Ardiani, Deasy Lusi Hidayati Lusi Hidayati Lusi Hidayati, Lusi Lutfi Meiga Sari Lutfia Siti Hanifatunnisa Mei Syafriadi Mochamad Nofal Abdulloh Muhammad Nurul Amin Mukti Aji Nugrah Ramadhan Nadza Qur’rotun A’ini Nanda Afnita Nugraha Agung Marda Nur Nafisah Wulandari Octaviana Putri Puspita Sari Oktavia Nur Vitasari Paramudibta Lungit Kuncaraningtyas Peni Pujiastuti Permata, Dea Permatasari, Sabania Dinar Permatasari, Yulita Indah Prafitasari, Dinar Pudji Astuti PUDJI ASTUTI Ratna Dewanti, I Dewa Ayu Ratu Millenia Dua Rendra Chriestedy Prasetya Rido Tri Andika Firdyansyach Riska Dwi Fajariyanti Roedy Budirahardjo Rothschildi, Dhesyarmani Putri Rudi Budirahardjo, Rudi Safira Niza Ulfita Safitri, Rizkiya Agnes Sari Setyaningsih Sartika Puspita Selvia Magdalena Setyaningsih, Sari Sri Lestari Suhartini Suhartini Suhartini Suhartini Syafira Az Zahro Tarmizi, Thariq Ibnu Tecky Indriana Tsalis, Dina Widodo, Alaya Dwi Salvahira Yani Corvianindya Rahayu Yunianti, Ervisya Nandya Zahara Meilawaty Zahara Meylawaty Zahreni Hamzah Zain, Adi Pradana Rahmatullah Zainul Cholid