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Impact of Dietary Coconut Oil (Cocos nucifera L.) on Aeromonas hydrophila Colonization and Lactic Acid Bacteria Populations in Zebrafish (Danio rerio): Implications for Aquaculture Health Qosimah, Dahliatul; Iwanegbe, Izuwa; Efendi, Achmad; Nasution, Angellica Christine; Hardiana, Ameilia; Zahrany, Naura Nisrina; Satria Putra, Pramadhita Wika; Daniswara, Respati Satya
Journal of Agriprecision & Social Impact Vol. 2 No. 1 (2025): March: JAPSI (Journal of Agriprecision & Social Impact)
Publisher : CV. Komunitas Dunia Peternakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62793/japsi.v2i1.44

Abstract

The Aeromonas hydrophila (A. hydrophila) Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen causing significant losses in aquaculture. The overuse of antibiotics has led to antimicrobial resistance, necessitating alternative control strategies. This study evaluates the potential of coconut oil to inhibit A. hydrophila colonization in gills, brain, and gut microbiota of zebrafish while enhancing lactic acid bacteria (LAB) populations. A completely randomized design was used with five groups: a negative control (healthy), a positive control (A. hydrophila-infected), and three treatment groups (1000 mg/kg, 2000 mg/kg, 4000 mg/kg coconut oil) for 60 days. Bacterial load in gills and brain was quantified using the pour plate method on Rimler-Shotts agar, while LAB in the gut was assessed using de Man, Rogosa, and Sharpe Agar (MRSA). 1000 mg/kg coconut oil significantly reduced A. hydrophila in the gills (0.3 ± 0.09 × 10⁷ CFU/ml) compared to the positive control (138.13 ± 11.26 × 10⁷ CFU/ml). 4000 mg/kg was most effective in reducing A. hydrophila in the brain (1.78 ± 0.51 × 10⁷ CFU/ml vs. 4.53 ± 0.40 × 10⁷ CFU/ml in the positive control). LAB populations significantly increased (p < 0.0001), with the highest count at 1000 mg/kg (9.20 ± 0.87 × 10⁷ CFU/ml), followed by 2000 mg/kg (3.16 ± 0.13 × 10⁷ CFU/ml), and 4000 mg/kg (1.78 ± 0.35 × 10⁷ CFU/ml). Coconut oil effectively enhances LAB populations, inhibits A. hydrophila colonization, and acts as a natural antimicrobial and probiotic agent. The 1000 mg/kg dose is optimal for gut microbiota improvement, while 4000 mg/kg protects brain tissues, highlighting coconut oil’s potential as an eco-friendly alternative to antibiotics in aquaculture.
Edukasi Perawatan Anjing dan Pencegahan Zoonosis di Kawasan Hutan dengan Tujuan Khusus (KHDTK) UB Forest : Dog Care Education and Zoonosis Prevention in the Special Purpose Forest Area (KHDTK) of UB Forest Dahliatul Qosimah; Mochammad Roviq; Siti Azizah; Ahmad Reiki Darmawan; Gresia Ivena; Khairunnisa Syadza Wally; Salsabila Dzakiyar Rusda; Ardian Febriansyah; Nelly Nur Agustina; Salsabila Putri Rahmadhanti; Aisya Luna Weningati; Aurevia Putri Ardi
Jurnal Kolaboratif Sains Vol. 8 No. 9: September 2025
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v8i9.8371

Abstract

Kawasan Hutan dengan Tujuan (KHDTK) UB Forest di Dusun Sumberwangi, Karangploso, dihuni komunitas petani yang memelihara anjing semi?liar sebagai penjaga rumah dan ladang. Literasi perawatan anjing dan pencegahan penyakit zoonosis yang rendah meningkatkan risiko penularan penyakit. Program pengabdian (Maret–Agustus 2025) oleh tim dosen–mahasiswa FKH UB bekerja sama dengan UPT KHDTK UB Forest bertujuan meningkatkan pengetahuan dan praktik warga melalui pendekatan kontekstual?partisipatif berbasis One Health dan nilai agama. Kegiatan mencakup penyuluhan interaktif; pelatihan mengenali karakter/bahasa tubuh anjing; perawatan dasar dan grooming; edukasi vaksinasi; simulasi penyucian najis; distribusi vitamin hewan serta serta pelatihan pembuatan gel antiseptik berbasis sereh–pinus. Sasaran utama adalah kelompok ibu?ibu, pemilik maupun non?pemilik anjing. Evaluasi melalui pre–post test, observasi, wawancara, dan Indeks Kepuasan Masyarakat menunjukkan peningkatan skor pengetahuan, sikap lebih positif terhadap anjing, serta adopsi praktik perawatan. Hambatan utama ialah keterbatasan akses layanan veteriner. Kesimpulannya, model edukasi komunitas ini layak, dapat diterima; berkontribusi pada SDGs 3 dan 15 serta memperkuat peran perguruan tinggi dalam kesehatan masyarakat pedesaan.
Review Peran suplemen probiotik dan herbal sebagai Antimikrobial, Antioksidan, and Imunomodulator terhadap Aeromonas hydrophila : Review artikel suplemen herbal dan probiotik untuk melawan Aeromonas hydrophila Qosimah, Dahliatul; Laminem; Mandasari, Candra; Setyawati , Dyah
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3621

Abstract

Aeromonas hydrophila (A. hydrophila) are classified as zoonotic and opportunistic bacteria. Natural immunostimulants such as herbs are biodegradable and safe for the environment and host health. There has been no systematic review of using these natural ingredients to treat or prevent A. hydrophila infection. This review aims to demonstrate the role of herbs and probiotics as antibacterial, antioxidant, and immunostimulatory against A. hydrophila infection. This study deployed systematic and comprehensive literature from PubMed, Google Scholar, and Scopus from 2012 to 2022, with keywords: A. hydrophila, herbal, probiotics, antioxidant, immunomodulator, antibacterial. Data are obtained from journals, proceeding of research results, excluding article reviews. The review results indicate that the utilization of these two ingredients (herbs and probiotics) is potential to prevent A. hydrophila infection in animal models with variation action mechanisms and dose administration. However, an inappropriate combination of herbs and probiotics leads to the development of resistance and toxicity profile, thereby requiring further research to standardize such combination prior to application for humans or animals.