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All Journal Jurnal Ilmu Pertanian Indonesia Microbiology Indonesia Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Prosiding Seminar Biologi Elkawnie Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati JPBIO (Jurnal Pendidikan Biologi) JKPK (Jurnal Kimia dan Pendidikan Kimia) Jurnal Biotek Jurnal Pengabdian Masyarakat Khatulistiwa Jurnal Triton Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Filogeni: Jurnal Mahasiswa Biologi
Sukmawaty, Eka
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Characterization of Soil Actinomycetes From Malino Pine Forest Rhizosphere of South Sulawesi Eka Sukmawaty; Sitti Rahmah Sari; Mashuri Masri
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 2 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.5383

Abstract

Abstract: Actinomycetes are a group of Gram-positive bacteria that produce active compounds with a wide distribution range in nature especially in soil. The purpose of this study was to isolate actinomycetes from the rhizosphere soil of the Malino pine forest, South Sulawesi. This research is a descriptive qualitative study of 15 actinomycetes isolated from the rhizosphere soil of the Pine Forest. These 15 isolates have been successfully identified to the genus level. Actinomycetes were isolated by direct dilution method and further morphological identification was carried out. Actinomycetes were isolated on yeast malt agar (YMA) medium. The growing isolates showed colony colors brown, gray, and white. Produces yellow, brown, and beige color pigments. Based on the characterization carried out, all isolates were identified into the genus Streptomyces.Abstrak: Aktinomisetes merupakan kelompok bakteri gram positif yang menghasilkan senyawa aktif dengan rentang distribusi yang luas di alam, terutama di tanah. Tujuan penelitian ini untuk mengisolasi aktinomisetes dari tanah rizosfer hutan pinus Malino Sulawesi Selatan. Penelitian ini merupakan penelitian kualitatif deskiptif terhadap 15 isolat aktinomisetes yang diisolasi dari tanah rizosfer Hutan Pinus Malino Sulawesi Selatan. 15 isolat ini telah berhasil didentifikasi sampai tingkat genus. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan dilakukan identifikasi morfologi lebih lanjut. Aktinomisetes diisolasi pada media yeast malt agar (YMA). Isolat yang tumbuh memperlihatkan warna koloni coklat, abu-abu dan putih. Menghasilkan pigmen warna kuning, coklat dan krem. Berdasarkan karakterisasi yang dilakukan keseluruhan isolat teridentifikasi ke dalam genus Streptomyces.
SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETIL ASETAT CENDAWAN ENDOFIT Aspergillus sp. Eka Sukmawaty; Hafsan Hafsan; Mashuri Masri; Inna Shintia; Sinar Wahyuni; Ulfa Nur Alfriani Amir
Biotik Vol 8, No 2 (2020): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v8i2.8194

Abstract

Cendawan endofit telah diketahui menghasilkan banyak senyawa bioaktif salah satunya senyawa antioksidan. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan  aktivitas antioksidan cendawan endofit Aspergillus sp. secara in vitro dan in vivo. Penentuan aktivitas antioksidan secara in vitro dilakukan dengan metode DPPH. Aktivitas antioksidan secara in vivo dilakukan dengan melihat kemampuannya menurunkan kadar MDA serum darah mencit yang diberi stress oksidatif. Hasil penelitian menunjukkan aktivitas antioksidan Ekstrak etil asetat Aspergillus sp. tergolong sangat kuat dengan IC50 sebesar 38,64, dan mampu menurunkan kadar MDA mencit pada konsentrasi 45 ppm/kgBB. Hasil uji fitokima menunjukkan terdapat kelompok senyawa Flavonoid, Alkaloid, Terpenoid dan Tanin yang dihasilkan dari ekstrak etil asetat cendawan endofit Aspergillus sp.
PENAMBATAN MOLEKULER SENYAWA CENDAWAN ENDOFIT Trichoderma sp. SEBAGAI INHIBITOR PROTEIN LOW DENSITY LIPOPROTEIN, ENZIM LANASTEROL 14 DEMETILASE DAN LIPASE YANG BERTANGGUNG JAWAB DALAM DERMATITIS SEBOROIK Eka Sukmawaty; Susi Susanti; Masriany Masriany Masriany
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 1 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.054 KB) | DOI: 10.36387/jiis.v6i1.636

Abstract

Seborrheic dermatitis (SD) occurs due to excessive sebum production resulting in the growth of the fungus Mellasezia sp. on the skin becomes uncontrollable. Some of the receptors that play a role in SD regulation are Low Density Lipoprotein (LDL) protein, Lanasterol 14-α Demetylase enzyme, and Lipase enzyme. This study aims to determine the potential compounds of the endophytic fungi Trichoderma sp. as an antiseborrheic and knowing the interaction between ligands as inhibitors in SD regulation. The research was conducted with computer-based molecular docking. The results showed that there were two inhibitor candidate compounds based on the selection by binding affinity value, namely Chloromycetin and Benzyl benzoate. Based on the results of docking with VINA autodock, the potential compounds were Benzyl benzoate on LDL protein with binding affinity values of -7.3 kcal/mol, compounds were Chloromycethin on Lipase enzyme with binding affinity values of -7.3 kcal/mol and Lanasterol 14-α demethylase enzyme with a binding affinity value of -7.6 kcal/mol. These compounds form hydrogen bonds and hydrophobic bonds. Based on the prediction of Lipinski's rule of five, the two compounds are effective orally used as anti seborrheic dermatitis drugs
DISKUSI ONLINE : MANAJEMEN REFERENSI (APLIKASI MENDELEY) DALAM PENULISAN KARYA ILMIAH Eka Astuty; Elpira Asmin; Eka Sukmawaty
Jurnal Pengabdian Masyarakat Khatulistiwa Vol 4, No 1 (2021): APRIL
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpmk.v4i1.866

Abstract

ABSTRAKSalah  satu  syarat yang harus dipenuhi oleh mahasiswa untuk mencapai gelar sarjana yaitu harus menulis karya  tulis  ilmiah  berupa  skripsi. Daftar pustaka dan sitasi menjadi elemen penting dalam penulisan karya ilmiah.  Penyusunan dua hal tersebut dapat dilakukan secara manual ataupun dengan bantuan aplikasi. Mendeley merupakan salah satu perangkat lunak sitasi yang cukup mudah penggunaannya bagi orang awam sehingga menjadi favorit penulis. Kegiatan ini dilakukan secara daring memakai aplikasi zoom sebab di masa pandemi Covid- 19,  kegiatan sosialisasi ataupun penyuluhan secara langsung tidak bisa dilakukan karena adanya anjuran Physical distancing. Selama berlangsungnya pemaparan materi, peserta diskusi dipersilahkan untuk mengemukakan pertanyaan. Peserta diskusi sangat antusias dan ada banyak pertanyaan yang disampaikan, diantaranya adalah sumber referensi yang bisa digunakan dalam penulisan karya ilmiah dan jenis-jenis gaya penulisan yang terdapat dalam aplikasi mendeley. Kegiatan pengabdian masyarakat dalam bentuk diskusi online tentang manajemen referensi (Aplikasi Mendeley) dalam penulisan karya ilmiah diharapkan dapat memberikan pengetahuan dan informasi terkhusus bagi peserta yang merupakan mahasiswa tingkat akhir. Diskusi online ini diharapkan juga menjadi wadah untuk me-refresh pengetahuan tentang manajemen referensi bagi peserta yang berprofesi sebagai dosen.Kata Kunci: Diskusi, Daring, Manajemen Referensi, Mendeley ABSTRACTOne of the requirements for students to achieve a bachelor's degree is to write scientific papers. Bibliography and citations are important elements in writing scientific papers. The arrangement of these two things is done manually or by application. Mendeley is a commonly used citation software. This activity was carried out online using the zoom application because during the Covid-19 pandemic, direct socialization or outreach activities could not be carried out because of the suggestion of Physical distancing. During the presentation of the material, discussion participants were invited to ask questions. The discussion participants were very enthusiastic and there were many questions submitted, including reference sources that could be used in writing scientific papers and the types of writing styles found in the Mendeley application. Community service activities in the form of online discussions about reference management (Mendeley Application) in writing scientific papers are expected to provide knowledge and information specifically for participants who are final year students. This online discussion is expected to also become a forum to refresh knowledge about reference management for participants who are lecturers. Keywords: Discussion, Online, Refference Management, Mendeley
KERAGAMAN MIKORIZA ARBUSKULA INDONESIA DAN PERANANANNYA DALAM EKOSISTEM Eka Sukmawaty; Asriani Asriani
Jurnal Biotek Vol 3 No 1 (2015): PROSIDING SEMINAR NASIONAL 22 Desember 2015
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7248.403 KB) | DOI: 10.24252/jb.v3i1.1901

Abstract

Cendawan Mikoriza Arbuskula (CMA) adalah salah satu kelompok cendawanyang hidup di dalam tanah, termasuk golongan endomikoriza yang mempunyaistruktur hifa yang disebut arbuskula sebagai tempat kontak dan transfer haramineral antara jamur dan tanaman inangnya pada jaringan korteks akar. Mikoriza terbentuk karena adanya simbiosis mutualisme antara cendawan atau fungi dengan sistem perakaran tumbuhan. Mikoriza arbuskula membentuk asosiasi simbiotik tertentu antara spesies tanaman dalam skala luas termasuk Angiosperm, Gymnosperm, Pterydopyta, dan beberapa Bryopyta, dan skala cendawan terbatas termasuk dalam ordo tunggal, Glomales. Cendawan ini dapat bersimbiosis dengan akar dan mempunyai peranan yang penting dalam pertumbuhan tanaman, baik secara ekologis maupun agronomis. Peran tersebut diantaranya adalah meningkatkan serapan fospor (P) dan unsur hara lainnya, seperti N, K, Zn, Co, S dan Mo dari dalam tanah, meningkatkan ketahanan terhadap kekeringan, memperbaiki agregasi tanah, meningkatkan pertumbuhan mikroba tanah yang bermanfaat bagi pertumbuhan tumbuhan inang serta sebagai pelindung tanaman dari infeksi pathogen akar. Selain itu mikoriza juga digunakan untuk remediasi lahan yang tercemar. Genus yang banyak dijumpai di Indonesia diantaranya yaitu Glomus, Gigaspora, dan Acaulospora.
ARBUSCULAR MYCORRHIZAL FUNGI (AMF) CHARACTERIZATION IN RHIZOSPHERE of Gigantochloa atter Fatimah Mansir; Hafsan Hafsan; Eka Sukmawaty; Masriany Masriany
Jurnal Biotek Vol 9 No 1 (2021): JUNI
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v9i1.20984

Abstract

Mycorrhizae are typical structures found in a plant's root system found symbiotic mutualism between fungi (myces) and roots (rhiza). Mycorrhizae have an essential role for plants because they can increase water and nutrient uptake, especially phosphorus absorption. The purpose of this study was to determine the mycorrhizal genera on the roots of parring bamboo plants (Gigantochloa atter) in Sabantang Hamlet, Toddopulia Village, Tanralili District, Maros Regency because there is no scientific information regarding this issue. This research was conducted with the wet filter method or sieving. The isolation of spores from the rhizosphere of Gigantochloa atter samples was carried out by referring to the pouring method and wet sieving using a stratified filter set. Staining techniques were used to observe the colonization of arbuscular mycorrhizal structures in the roots of the sample plants. The results showed that 27 spores were found, consisting of 15 spores of the Glomus genera, three spores of the Gigaspora genera, and nine Acaulospora genera. Observation of the root structure has not shown the presence of vesicular and arbuscular. However, only hyphae and spore structures were found because the mycorrhizal hyphae in Gigantochloa atter have not yet reached the infection stage to form arbuscular or vesicular structures
UJI FITOKIMIA EKSTRAK ETANOL BUNGA POHON HUJAN (Spathodea campanulata) SECARA IN VITRO Widyawati jafar; Masriany Masriany; Eka Sukmawaty
Prosiding Seminar Nasional Biotik Vol 8, No 1 (2020): PROSIDING SEMINAR NASIONAL BIOTIK VIII 2020
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh, Aceh, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.827 KB) | DOI: 10.22373/pbio.v8i2.9658

Abstract

Pohon hujan (Spathodea campanulata) merupakan tumbuhan yang tersebar diberbagai Negara tropis salah satunya ialah Indonesia. Selama ini tumbuhan Spathodea campanulata digunakan sebagai pengobatan tradisional oleh masyarakat. Berdasarkan tinjauan literatur tumbuhan ini mengandung banyak senyawa aktif yang berpotensi sebagai bioaktivitas. Penelitian ini bertujuan untuk mengetahui senyawa metabolit sekunder yang terdapat pada bunga pohon hujan (Spathodea campanulata) yang sudah mekar dan bunga pohon hujan (Spathodea campanulata) yang masih kuncup. Sampel pada penelitian ini diperoleh dari Kota Malino, Kab. Gowa yang kemudian dilakukan skrining fitokimia. Hasil yang diperoleh dari penelitian yaitu ditemukannya senyawa metabolit sekunder flavonoid, alkaloid, terpenoid dan tanin pada kedua sampel yang diujikan. Adanya kandungan metabolit sekunder terhadap bunga pohon hujan (Spathodea campanulata) yang sudah mekar dan Bunga pohon hujan (Spathodea campanulata) yang masih kuncup menunjukkan bahwa bunga ini berpotensi dikembangkan untuk pengujian bioaktivitas, yang mencakup anti bakteri, anti jamur, anti kanker dan anti oksidan.
Antibacterial effect of ethanol extract of nutmeg against foodborne disease pathogens Eka Astuty; Eka Sukmawaty
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1856

Abstract

Foodborne disease is a disease that occurs as a result of food contamination by microbes or harmful chemicals. Nutmeg (Myristica fragrans Houtt.)  is widely used as a spice and flavor enhancer for food and beverages. In addition, it has traditionally been used to treat diarrhea and kidney disease. Several pharmacological studies have reported the antioxidant, antimicrobial, antidiarrheal and anti-inflammatory activity of this plant. Antibacterial effectiveness test of nutmeg against several pathogens in this study was carried out using the disc diffusion method. The results of this study indicated that the ethanol extract of flesh of fruit (concentration of 5%, 10%, 15%, 20%, and 25%) had antibacterial activity against all tested pathogenic bacteria. Different results were shown by the ethanol extract of seed and mace. All the ethanol extract concentrations of seed were not able to inhibit the growth of Shigella sp. and Clostridium sp., while the ethanol extract of mace in all concentrations was unable to inhibit the growth of Shigella sp. Various factors may affecting this extract and compound activity when used in complex biological systems such as in vivo and human studies. Future studies must also focus on aspects of pharmacokinetics and toxicological plant extracts and phytochemicals.
Molecular Docking of Shallot (Allium ascalonicum) Active Compounds to Lanosterol Enzym 14-Alpha Demethylase and Squalene Monooxygenase for Antifungi Potential Activity Eka sukmawaty; Nur Alfisah; Masriany Masriany; Hafsan Hafsan
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 7, No 3 (2022): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v7i3.45999

Abstract

Shallots (Allium ascalonicum) are one of the important horticultural export commodities in Indonesia, but the productivity of shallots has decreased due to the attack of pathogenic fungi. This study was aimed to determine the potential of active shallot compounds as ligands for inhibiting the activity of the enzyme lanosterol 14-alpha demethylase (ID: 4LXJ) and squalene monooxygenase (ID: 6C6R) enzymes which play an important role in the biosynthesis of ergosterol or fungal cell membranes. The molecular docking used the Autodock Vina (PyRx) program, an analysis of molecule interaction used PyMol, and Discovery Studio 2019 to analyze the types of bonds between molecules. Thirty-nine ligands from shallot compounds are docked to the anti-fungal target protein. The results showed ascalonicoside A1, ascalonicoside A2, ascalonicoside B, quercetin, isorhamnetin, quercetin di glucoside, quercetin tri glucoside, ethyl palmitate and benzyl salicylate have the potential to be anti-fungal to the enzyme lanosterol 14-alpha demethylase and squalene monooxygenase enzyme that responds to the synthesis of fungi cell wall. These ligand compounds bind to the target protein's amino acid residues with hydrogen and hydrophobic bonds. This research showed that shallot was the potential to be replicated as an anti-fungus for many purposes.
Anti Fungal Activity of Chitinolytic Bacteria Lysinibacillus fusiformis and Brevibacillus reuszeri Against The Fungal Pathogens Rhizoctonia solani and Fusarium oxysporum Mashuri Masri; Eka Sukmawaty; As Awalia Amir
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.924 KB) | DOI: 10.5454/mi.15.4.3

Abstract

Abstract. Chitinolytic bacteria can produce chitinase, reported as a biocontrol agent against plants. This research aims to see chitinolytic activity in inhibiting the growth of Rhizoctonia solani and Fusarium oxysporum. Anti fungal testing in dual culture test by growing each of the chitinolytic bacteria, Lysinibacillus fusiformis and Brevibacillus reuszeri, with the pathogenic fungi, F. oxysporum and R. solani, in Petri dishes containing Chitin Agar Media facing a distance of 3 cm. The results showed that chitinolytic bacterial isolates were capable inhibit the fungus by having the activity of each index inhibition of L. fusiformis isolates (30%), B. reuszeri (77%) against F. oxysporum, and R. solani fungi isolates (100%) for each chitinolytic bacterial isolate. Keywords : Anti fungal, Chitinolytic bacteria, Pathogenic fungi.
Co-Authors Abdul Karim Ahsan, Andi Widya Aulia Ahyar Ahmad Annisa, Andi Bunga Sari As Awalia Amir Asmuddin Natsir Asriani A Asriani Asriani Cut Muthiadin Devi Armita dwyana, Zarsawaty Eka Astuty Elpira Asmin Fatimah Mansir Fatmawati Nur Fuadi, Ni'ma Ainul Hafsan, Hafsan Harningsih Karim Hasnah Natsir Inna Shintia Isbatullah, Muhammad Isna Rasdianah Aziz Laily Agustina Mashuri Masri Mashuri Masri MASHURI MASRI Mashuri Masri Mashuri Masri Mashuri Masri - Maslan Maslan Masriany Masriany Masriany Masriany Masriany Masriany Masriany Masriany, Masriany Meriem, Selis Musdalifah Musdalifah Nur Alfisah Nurhidayah Nurhidayah Nurhikmah Nurhikmah Nurul Afriani Arif NURZAKIYAH NURZAKIYAH Pratama, Bayu Arief Rani, Awalia Ramadani Rezki Ramadani Djamaluddin Risman Rifaldi Rusli, Jumriani Sakinah Sakinah Samsuar Manyyulei Sinar Wahyuni Siti Patima Sitti Rahmah Sari SRI UTAMI PUTRI Suriani Suriani Susi Susanti Ulfa Nur Alfriani Amir Ulfa Triyani A. Latif Venny Dwi Cahyani Wahab, Andi Rizki Berliana Zahra Wamaulana, Farlinda Widyawati jafar Yuniar Harviyanti ZURAIDAH ZURAIDAH