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All Journal PROSIDING SEMINAR NASIONAL CommIT (Communication & Information Technology) agriTECH EduChemia: Jurnal Kimia dan Pendidikan PEMBELAJAR: Jurnal Ilmu Pendidikan, Keguruan, dan Pembelajaran Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Journal of Information System Indonesian Journal of Chemistry PROSIDING SEMINAR NASIONAL PROGRAM PASCASARJANA Molekul: Jurnal Ilmiah Kimia Jurnal Biomedika International Journal of Business, Humanities, Education and Social Sciences (IJBHES) Berkala Penelitian Hayati COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Indonesia Sosial Teknologi Jurnal Cakrawala Bahari
Tri Joko Raharjo
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Gadjah Mada, Sekip Utara, 55281 Yogyakarta, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Journal : PROSIDING SEMINAR NASIONAL

 1 
UJI STABILITAS ENZIM LIPASE TERIMOBILISASI PADA KITOSAN SERBUK MELALUI TEKNIK TAUT SILANG Fandhi Adi Wardoyo; Tri Joko Raharjo; Respati Tri Swasono
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Lipase has a great catalytic power, but easily influenced by environments and difficult to separate at the ends of the reaction, so it can not be re-used. Based on that, one solution to overcome that problem is through the enzyme immobilization. Immobilization of lipase on chitosan powder was performed via cross linking technique. Chitosan was prepared from crab shell with deacetylation degree 82,34% and crosslinked with glutaraldehide. The aims of this research is to determine the thermal stability and multiple-use stability through the hydrolysis reaction of palm oil. Immobilized lipase on chitosan powder was able to maintain its activity at 50 oC, and still can produce free fatty acid as much as 48,55%. The immobilized lipase was also able to maintain its activity after multiple-uses up to five reaction cycles, and still can produce free fatty acid as much as 40,82%.Keywords: lipase, immobilization, chitosan, cross linking
Co-Authors Agus Zulkarnain, Agus Alfiraza, Ery Nourika Andika, Rio Ani Setyopratiwi Arif Mulyanto, Arif Ariyani Setyo Widhiyati Arwansah, Arwansah Atmadja, Wiedjaja Bambang Sutriyanto Chairil Anwar Chairil Anwar Condrobimo, Andreas Raharto Dendang, Adrianto Deni Pranowo Deni Pranowo Dilin Rahayu Nataningtyas Diptyo Hisnuaji, Diptyo Dwi Hudiyanti Eka Widjaja, Henry Antonius Elly Rustanti Endah Mulya Asih Endang Semiarti Endar Marraskuranto Esti Enjelina Fandhi Adi Wardoyo Garnis Putri Erlista Gilang Aji Pratama Harisman Jaya, Harisman Hasnur, Juliandri Hidayat, Panca Nur Iqmal Tahir Irma Nuryanti Istini Istini Istini Istini Jaka Widada Jatmiko, Fauzi Kurniawan, Fika L, Bagus Budhi Bowo L. Hartanto Nugroho Laily Nurliana Mahargyani, Wikan Mai Anugrahwati Markus Asta Patma Nugraha Maulidiyawan, Robby Meyliana Mudasir Mudasir Mudasir Narsito Narsito Narsito Narsito Naseer Ahmed Nugroho, Edi Setyawan Nuniek Herdyastuti Nurul Hidayat Aprilita Oedjijono Oedjijono Ogbuadike Eucharia Patricia Lubis, Patricia Puji Lestari Puji Lestari Raden Tambunan Rarastoeti Pratiwi Respati Tri Swasono Rina Sri Kasiamdari Robert Verpoorte Rosyida Azis Rizki Rusmiati Suprihatin S, Aryo Adi Sabirin Mastjeh Sabirin Matsjeh Sablan, Bruno Santi Nur Handayani Saputra, M. Ferry Sari Edi Cahyaningrum Shalehuddin, Hasnan Habib Sismindari Sismindari Siti Nuryanti Siti Nuryanti Slamet Raharjo Sonny Ridwanto, Sonny Sri Noegrohati Sri Noegrohati Stalis Norma Ethica Suhirwan, Suhirwan Sumiaty Sumiaty Sunusi, M. Syafril Sunusi, Sahabuddin Surajiman Surajiman Surajiman Surajiman Surjandy Susan Primadevi Tri Rini Nuringtyas Tri Wahyudi Tutik Dwi Wahyuningsih Wen-Te Chang Winarno Winarno Winarto Haryadi Winda Cahyaningtyas Yohannes Kristanto, Yohannes