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All Journal Jurnal Pendidikan Kewarganegaraan Undiksha Media Penelitian dan Pengembangan Kesehatan Dentika Dental Journal Jurnal Komunikasi Hukum Elkawnie The Indonesian Biomedical Journal Science and Technology Indonesia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Biota Oto Rhino Laryngologica Indonesiana Kosmik Hukum Ganesha Law Review BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Daniel Joko Wahyono
Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Jawa Tengah, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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JURIDICAL REVIEW OF LEGAL PROVISIONS FOR THE IMPLEMENTATION AND UTILIZATION OF THE CONVERSION OF FOREST LAND INTO CAPITALISTS-ORIENTED PLANTATIONS Elly Kristiani Purwendah; Daniel Joko Wahyono
Ganesha Law Review Vol 6 No 2 (2024): November
Publisher : Program Studi Ilmu Hukum, Jurusan Hukum dan Kewarganegaraan, Fakultas Hukum dan Ilmu Sosial, Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/glr.v6i2.4166

Abstract

This research aims to find out how the legal provisions for the implementation and use of land conversion into plantation forests are capitalistic oriented. This research uses a normative juridical research method with a type of approach, namely a statutory and regulatory approach and a conceptual approach. The legal materials used are primary, secondary and tertiary legal materials, obtained by conducting literature studies. The results of the research show that the conversion of forest land into plantation land is stated in Government Regulation Number 64 of 1957 concerning Forestry Deregulation, Law Number 5 of 1967 concerning Forestry and was later replaced by Law Number 41 of 1999. In its implementation the existing regulations It turns out that this has not been rooted in the legal ideology, social system and political system of the constitution, and the Basic Agrarian Principles regulations. It seems that the use of forests that is managed and oriented towards capitalists cannot be used as a measure of the government's success in economic development, considering that there are still many inequalities and problems that have occurred so far. Considering the negative impacts rather than the function of land which is increasingly expanding and wasted.
The Effect of Pretreatment Temperature in The Mycobacterium tuberculosis Extraction Method on DNA Quality Setianingsih, Tri Yuli; Fitri, Safira; Wahyono, Daniel Joko; Setiawaty, Vivi
Elkawnie: Journal of Islamic Science and Technology Vol 10, No 2 (2024)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v10i2.23062

Abstract

Abstract: The existing DNA extraction process for Mycobacterium tuberculosis employs a pretreatment step that may not function at its full potential. This inefficient process led to complications in subsequent analysis; thus, it is necessary to determine the successful extraction method with or without adding a pretreatment step. This study aims to determine the effect of temperature pretreatment on the extraction method of M. tuberculosis on the quality of DNA for WGS examination. The research design was analytically descriptive with a kit-based extraction method from ExiPrepTM Dx Mycobacteria Genomic DNA Kit (K-4418), Bioneer. The results of this study were that DNA purity did not differ (p = 0.959) between the pretreatment heating at 950 °C (2.88) and no heating at 950 °C (2.46). DNA concentration did not differ (p-value = 0.111) between pretreatment heating 950C (0.416 ng/µL) and no heating 950C (0.653 ng/µL). The relatively low DNA concentration resulted in the DNA bands not being visualized on agarose gel electrophoresis (0.8%). In conclusion, our study showed that the pretreatment heating step on M. tuberculosis DNA extraction does not affect DNA purity, concentration, or integrity. This inefficiency step can be discarded from the M. tuberculosis DNA extraction process to simplify the extraction processing. However, other extraction kit approaches need to be explored in future studies.Abstrak: Proses ekstraksi DNA Mycobacterium tuberculosis yang saat ini dilakukan memerlukan pretreatment yang mungkin tidak berpengaruh secara optimal. Proses yang tidak efisiensi berpengaruh terhadap analisis selanjutnya, sehingga perlu untuk mengetahui keberhasilan metode ekstraksi dengan penambahan proses pretreatment. Tujuan penelitian ini adalah untuk mengetahui pengaruh pretreatment suhu pada metode ekstraksi M. tuberkulosis terhadap kualitas DNA untuk pemeriksaan WGS. Desain penelitian adalah deskriptif analitik dengan metode ekstraksi berbasis kit dari ExiPrepTM Dx Mycobacteria Genomic DNA Kit (K-4418), Bioneer. Hasil penelitian adalah kemurnian DNA tidak berbeda (p=0,959) antara pretreatment pemanasan 950C (2,88) dengan tanpa pemanasan 950C (2,46). Konsentrasi DNA tidak berbeda (p value = 0,111) antara pretreatment pemanasan 950C (0,416 ng/µL) dengan tanpa pemanasan 950C (0,653 ng/µL). Konsentrasi DNA yang relatif rendah mengakibatkan pita DNA tidak tervisualisasi pada elektroforesis gel agarosa (0,8%). Kesimpulan dari penelitian  ini, bahwa langkah pemanasan awal pada ekstraksi DNA M. tuberkulosis tidak berpengaruh terhadap kemurnian, konsentrasi, dan integritas DNA. Langkah ini dapat dihilangkan dari proses ekstraksi DNA M. tuberkulosis untuk menyederhanakan proses ekstraksi. Namun, pendekatan kit ekstraksi lainnya perlu dieksplorasi dalam penelitian selanjutnya
Mitigation Effort for Sustainable Mangrove Management Towards a Low Emission Indonesia: Study at Arboretum Mangrove Kolak Sekancil Purwendah, Elly Kristiani; Triana, Ikama Dewi Setia; Pudyastiwi, Elisabeth; Kusumaningsih, Oti; Wahyono, Daniel Joko
Kosmik Hukum Vol. 25 No. 1 (2025)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/kosmikhukum.v25i1.24595

Abstract

Krida Wana Lestari Farmer Group supports the Indonesian government's policy to reduce carbon emissions as a community obligation to participate in managing the environment, as the purpose of the ratification of the Paris Agreement ( Law Number 16 of 2016 on the Ratification of Paris Agreement to the United Nations Framework Convention on Climate Change). The farmer group successfully built an edu-tourism area, the Segara Anakan Lagoon Conservation Mangrove Arboretum (Kolak Sekancil). This farmer group cultivates and utilizes mangrove products for life and has succeeded in increasing mangrove density in the Cilacap Sea Village Area with an area of approximately 6,2126.28 Ha. Mangrove farming by farming communities is a form of community participation regulated in Article 65 Paragraph (4) Law Number 32 of 2009 on Environmental Protection and Management The legal institution of this community is formally supported by the village government with Village Regulation No. 7 of 2022 concerning Mangrove Ecosystem Management in the Segara Anakan Region of Cilacap.
Advancements in Real-Time PCR Technologies: A Comprehensive Review of Probe-Based and Non-Probe-Based Assays for Molecular Diagnostics Malau, Jekmal; Zahro, Aurora Fatimatuz; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Meilani, Nanda Diva; Damara, Dandy Satria; Lestari, Agatha Nabilla; Saryono; Wahyono, Daniel Joko
Science and Technology Indonesia Vol. 10 No. 3 (2025): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.3.660-677

Abstract

The decision between probe-based and non-probe-based qPCR assays is crucial, influenced by diagnostic goals and sample characteristics. This review provides an in-depth evaluation of these two assay types, analyzing their principles, strengths, drawbacks, and applications. A thorough review of the literature, primarily sourced from PubMed, was undertaken to explore prominent assay systems, including TaqMan, KASP, rhAmp, HRM, and SYBR Green. Probe-based qPCR assays, exemplified by TaqMan and rhAmp, are distinguished by their high specificity, aptitude for multiplex analysis, and reduced risk of false positives, making them highly suitable for SNP genotyping and pathogen detection. However, their elevated costs and intricate design requirements remain significant challenges. Conversely, non-probe-based assays, such as SYBR Green and HRM, present cost-effective alternatives with straightforward designs. HRM, in particular, is effective in identifying genetic variations like SNPs with remarkable sensitivity. Nonetheless, these methods are susceptible to non-specific amplifications, requiring careful optimization to maintain reliability. The selection of a suitable qPCR assay depends on various factors, including precision, affordability, and multiplexing capabilities, with applications spanning infectious disease detection and genetic disorder analysis. This review emphasizes the indispensable role of qPCR in molecular diagnostics while showcasing recent technological advances that aim to mitigate existing constraints and enhance diagnostic precision and accessibility.
Regional Variations in rpoB Gene Mutations and Their Association with Rifampicin Resistance in Mycobacterium tuberculosis Widodo, Widodo; Wahyono, Daniel Joko; Oedjijono, Oedjijono; Pramono, Hendro
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.13215

Abstract

ABSTRACT. The increasing incidence of tuberculosis (TB) remains a global challenge, driven by the emergence of Mycobacterium tuberculosis resistance to antituberculosis drugs. This study investigated the relationship between rpoB gene mutations, rifampicin resistance levels, and the geographic origin of multidrug-resistant M. tuberculosis (MDR-TB) isolates. A total of 30 MDR-TB isolates were collected from the Central Java Provincial Health and Calibration Testing Laboratory between January and December 2023. Rifampicin resistance levels ranged from 5 to 80 µg/mL and were assessed using the microculture method, while DNA sequencing identified rpoB mutations. The results showed that rifampicin exposure significantly influenced rpoB mutation frequencies, with Ser531Leu (50%), His526 (16.7%), Leu511 (6%), Leu533 (6%), and Gln513 (6%) being the most common. Geographic variations were observed, with isolates from the ex-residencies Pekalongan, Pati, and Banyumas Residencies harboring seven, six, and four distinct mutations, respectively. Mutations such as Leu511Arg+His526Ser and Asp516Val were detected at low rifampicin concentrations (5–10 µg/mL), whereas Ser531Leu mutations dominated at moderate levels (20–80 µg/mL). These findings confirmed that rpoB mutations were influenced by both the region of origin and rifampicin resistance levels, providing critical insights for improving TB diagnosis and optimizing MDR-TB treatment strategies. Keywords: Mycobacterium tuberculosis, rifampicin resistance, rpoB gene mutation
WASTE BANK AS AN ALTERNATIVE TO COMMUNITY-BASED WASTE MANAGEMENT Elly Kristiani Purwendah; Daniel Joko Wahyono
Jurnal Komunikasi Hukum Vol 8 No 2 (2022): Agustus, Jurnal Komunikasi Hukum
Publisher : Program Studi Ilmu Hukum Fakultas Hukum dan Ilmu Sosial Universitas Pendidikan Ganesha Singaraja

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jkh.v8i2.47084

Abstract

This study aims to (1) find out and analyze how the waste bank program is managed, and (2) find out and analyze how the benefits of the waste bank program are as an effort to empower the community. The type of research used is normative legal research. The data obtained from this study were secondary data obtained through a literature study, then the data obtained were analyzed qualitatively juridically through a statutory and conceptual approach. The results of this study indicate that (1) the Waste Bank in its implementation can reduce the high number of waste in the community and in the final disposal site (TPA). Thus, through the Waste Bank, it is an alternative solution for the government and the community in reducing the increasing volume of waste. (2) Community-based waste management as an approach to waste management based on the active participation of the community. Environmental management requires the facilitation and implementation of community-based efforts as a strategy for empowering and increasing their access to environmental resources. The benefit of the Waste Bank for the community is that it can increase people's income because when they exchange their waste they will get a reward in the form of money collected in the account they have.
Co-Authors AGUS HERY SUSANTO Ahsanal Kasasiah Andreanne Poppy Estania Anton Budhi Darmawan Arie Ardiansyah Nugraha Ashar, Fadli Bambang hermani Damara, Dandy Satria Dewi Setia Triana, Ikama Dodi Safari Dodi Safari Dody Novrial, Dody Elly Kristiani Purwendah ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH Fitri, Safira Fuadi, Muflih Gumilas, Nur Signa Aini Haris Budi Widodo Hendro Pramono Hendro Pramono Hidayat Sulistyo Irfan Dwiandhono Jekmal Malau Korrie Salsabila Kusumaningsih, Oti Leader Alfason Lestari, Agatha Nabilla Lilis Mulyani Maretra Anindya Puspaningrum Meilani, Nanda Diva Miranti Oviani Nawangtantrini, Gita Nuniek Ina Ratnaningtyas Nur Signa Aini Gumilas Oedjijono Oedjijono, Oedjijono Oviani, Miranti Prayitno, Priskila Agnesia Priskila Agnesia Prayitno Pudyastiwi, Elisabet Purnomo Soeharso Reinhard Simbolon Rokhmani Rokhmani Rokhmani Rokhmani, Rokhmani Roni Afriansya Safari, Dodi Saryono Setianingsih, Tri Yuli Setyawati, Endang Ariyani Siwi Pramatama Mars Wijayanti Sulistiyawati, Indah Tri Yulia Ningsih Vivi Setiawaty Vivi Setiawaty Wahyu Siswandari Widodo Widodo Wisiva Tofriska Paramaiswari Zahra, Aliya Azkia Zahro, Aurora Fatimatuz