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All Journal Jurnal Pendidikan Kewarganegaraan Undiksha Media Penelitian dan Pengembangan Kesehatan Dentika Dental Journal Jurnal Komunikasi Hukum Elkawnie The Indonesian Biomedical Journal Science and Technology Indonesia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Biota Oto Rhino Laryngologica Indonesiana Kosmik Hukum Ganesha Law Review BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Daniel Joko Wahyono
Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Jawa Tengah, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java Rokhmani Rokhmani; Daniel Joko Wahyono; Lilis Mulyani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3924

Abstract

Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.
Ekspresi Relatif mRNA BRLF1 Epstein-Barr Virus dari Biopsi Jaringan Tumor dalam Blok Parafin sebagai Petanda Biologi Patogenesis Karsinoma Nasofaring Andreanne Poppy Estania; Hidayat Sulistyo; Daniel Joko Wahyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.409

Abstract

Karsinoma nasofaring (KNF) merupakan tumor ganas pada sel epitel nasofaring dan merupakan penyakit multifaktor yang bersifat endemik. Tipe KNF tidak berdiferensiasi (KNF WHO-3) telah terbukti konsisten dengan infeksi EBV. Gen litik BRLF1 berperan sebagai mediator transisi dari fase laten menjadi litik. Pengukuran aktivitas mRNA EBV di lokasi tumor primer perlu dilakukan karena lebih mencerminkan patogenesis KNF yang  sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi darah. Penelitian ini bertujuan untuk mengetahui nilai ekspresi relatif mRNA BRLF1 Epstein-Barr Virus pada beberapa tingkat stadium tumor penderita KNF sebagai  petanda biologi potensial dalam patogenesis KNF. Metode penelitian yang digunakan adalah metode deskriptif yang berbentuk studi seran lintang. Sampel penelitian ini adalah biopsi jaringan tumor dalam blok parafin penderita KNF sebanyak 24 sampel. Sampel tersebut telah didiagnosis pasti sebagai KNF WHO-3 dari hasil pemeriksaan Patologi Anatomi pada Poli Patologi Anatomi, RSUD Prof. Dr. Margono Soekarjo, Purwokerto serta memenuhi kriteria inklusi sampel. Analisis statistik dilakukan untuk membandingkan ekspresi relatif mRNA BRLF1 stadium yang berbeda. Nilai ekspresi relatif mRNA BRLF1 EBV pada 24 sampel KNF berkisar 99,04415959-1097,496026. Hasil analisis statistik menunjukkan nilai ekspresi relatif mRNA BRLF1 EBV pada stadium awal (n = 5; 544,72420 + 142,614733) lebih tinggi 1,8 kali dibandingkan dengan stadium lanjut (n = 17; 395,68612 + 293,172201), namun peningkatan  ekspresi relatif mRNA BRLF1 EBV tidak bermakna secara statistik karena didapatkan nilai p=0,130 (p>0,05). Oleh karena itu, ekspresi relatif mRNA BRLF1 EBV dari biopsi jaringan tumor dalam blok parafin tidak berpotensi sebagai petanda biologi molekul patogenesis KNF, khususnya progresivitas tumor pada stadium lanjut KNF.
Deteksi Gen Litik BRLF1 Epstein-Barr Virus pada Penderita Karsinoma Nasofaring Tri Yulia Ningsih; Daniel Joko Wahyono; Nur Signa Aini Gumilas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.517

Abstract

Rosenmuller fossa. Epithelial malignancy is often found in Chinese populations and Southeast Asia including Indonesia. Undifferentiated nasopharyngeal carcinoma (NPC WHO-3) type is 100% associated with Epstein-Barr virus (EBV) infection. Bam-HI R Leftward Reading Frame 1 (BRLF1) lytic gene has an important function as a transition mediator of latent phase to the lytic phase in EBV cycle. Detection of BRLF1 gene by PCR can be used for NPC diagnosis. The aim of this study is to identify BRLF1 lytic genes as molecular markers of Epstein-Barr virus in nasopharyngeal carcinoma patients with conventional PCR method and to determine the sensitivity of conventional PCR method to detect BRLF1 gene. The research design was cross sectional study. A total of 22 DNA samples were isolated from venous blood of NPC patients from RSUD Prof dr Margono Soekarjo, Purwokerto with informed consent. BRLF1 gene identification is done with conventional PCR technique. The results of this research showed that BRLF1 genes as molecular markers lytic cycle of Epstein-Barr virus in nasopharyngeal carcinoma patients can be identified conventional PCR technique that will produced DNA 157 bp. BRLF1 gene was detected in 16 samples (72.73%) of 22 samples of this study.
Ekspresi mRNA LMP2A Epstein-Barr Virus dari Biopsi Jaringan dalam Blok Parafin Berpotensi sebagai Biomarka dalam Diagnosis Karsinoma Nasofaring Roni Afriansya; Hendro Pramono; Hidayat Sulistyo; Daniel Joko Wahyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.323

Abstract

Karsinoma nasofaring (KNF) merupakan tumor epitel yang terletak di nasofaring dan merupakan penyakit genetik multifaktor yang endemik. Penyebab utama KNF adalah infeksi oleh Virus Epstein-Barr (EBV). Keberadaan EBV pada penderita KNF dapat diketahui dengan ditemukannya DNA EBV dalam spesimen biopsi jaringan penderita KNF. Genom EBV dan ekspresi sebagian dari produk gen laten virus secara konsisten terdeteksi hampir di setiap sel dalam kanker ini, salah satunya adalah gen Latent Membrant Protein (LMP). Aktivitas mRNA EBV lebih mencerminkan patogenesis KNF yang  sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi. Penelitian dilakukan di Laboratorium PA RSUD Margono Seokarjo, Purwokerto/Lab PA Fakultas Kedokteran dan laboratorium genetika/molekuler Universitas Jenderal Soedirman Purwokerto. Analisis Ekspresi mRNA LMP2A EBV dengan teknik one step RT-PCR dan produk RT-PCR (amplikon cDNA) divisualisasi dengan elektroforesis gel agarosa 1%. Hasil ekspresi mRNA LMP2A EBV adalah 27,3% (6 dari 22 sampel). Kesimpulan, metode one step RT-PCR dapat digunakan untuk menganalisis ekspresi mRNA LMP2A EBV dari sampel biopsi jaringan KNF dalam blok paraffin dan hasil positivitas ekspresi mRNA LMP2A EBV sedang, sehingga berpotensi digunakan sebagai petanda biologi molekul diagnosis KNF.
Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae Sulistiyawati, Indah; Wahyono, Daniel Joko; Siswandari, Wahyu
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.10134

Abstract

Porin messenger RNA (mRNA) expression significantly influences porin permeability and reduces antibiotic penetration. Klebsiella pneumoniae has developed resistance to several antibiotics. Decreased expression levels of OmpK35 and OmpK36 porins result in changes in the porin profile and even functional loss of porins. This research aimed to analyze the relative expression of the outer membrane porins, OmpK35 and OmpK36. The research methodology involved MIC assays and quantification of OmpK35 and OmpK36 mRNA using RT-qPCR. The relative expression of OmpK35 and OmpK36 in clinical isolate K. pneumoniae is 0.8925 and 0.5877, respectively. The porin permeability of OmpK35/K36 mRNA positively correlated with the MIC values of OmpK35 (p-value = 0.029) and OmpK36 (p-value = 0.016), respectively. Keywords: Ciprofloxacin, Klebsiella pneumoniae, OmpK35/K36, porin permeability
Identifikasi Serotipe dan Sensitivitas Antibiotik S. pneumoniae yang Dibawa Nasofaring Penderita Oma di Kabupaten Banyumas Oviani, Miranti; Wahyono, Daniel Joko; Safari, Dodi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1779

Abstract

The aims of this study are to detect S. pneumoniae carried by nasopharnyx of children in primary school (aged 6-12 years) that diagnosed with AOM, to identify sensitivity of S. pneumoniae to antibiotics. The design of this study is nonexperimental survey with the descriptive analysis. Sampling was conducted in September - December 2018 in Banyumas district primary schools. Detection of S. pneumoniae was performed with microbiology methods. Meanwhile, serotype was determined by multiplex PCR and sensitivity to antibiotics was deduced using disc diffusion. The result of this study showed that carriage rate of S. pneumoniae carried by nasopharynx in children aged over 5 years that diagnosed with AOM in the Banyumas district was 35%. Serotype of S. pneumoniae obtainend from this study were 6A/6B, 6C/6D, 17F, 3, 13, 14, 23B, and untypeable. In addition, isolates of S. pneumoniae were highly susceptible to clindamycin (100%), erythromycin (100%), sulphametaxazole/trimethoprim (100%), chloramphenicol (88%), oxacillin (77%), and tetracycline (66%).
DETEKSI MOLEKULER VIRUS DENGUE SEROTIPE 3 PADA NYAMUK Aedes aegypti DI WILAYAH PURWOKERTO TIMUR Prayitno, Priskila Agnesia; Kusmintarsih, Endang Srimurni; Wahyono, Daniel Joko
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1826

Abstract

Penyakit Demam Berdarah Dengue (DBD) adalah penyakit menular yang disebabkan oleh virus Dengue dan ditularkan melalui gigitan nyamuk Aedes aegypti. Nyamuk tersebut merupakan vektor utama dalam penularan virus Dengue (DENV) dengan ciri khas tubuh dan tungkainya ditutupi sisik dengan garis-garis putih keperakan. Persebarannya luas di daerah tropis dan subtropis, Purwokerto termasuk daerah endemis DBD dan sekaligus ditemukan nyamuk sebagai vektornya. Kecamatan Purwokerto Timur menempati urutan tertinggi dari banyaknya kejadian DBD di wilayah Banyumas, bahkan kejadian luar biasa (KLB) yang terjadi di Kelurahan Sokanegara pada tahun 2016 hingga menyebabkan kematian. Penelitian sebelumnya melaporkan bahwa virus Dengue yang paling banyak ditemukan di Purwokerto adalah serotipe 3. Oleh karena itu, deteksi molekuker nyamuk Ae. aegypti perlu dilakukan berkaitan dengan prediksi penularan Dengue untuk memperoleh informasi awal dalam pencegahan dan pengendalian DENV.Tujuan penelitian ini adalah untuk mendeteksi virus Dengue serotipe 3 pada nyamuk dewasa Ae. aegypti sebagai vektornya. Metode penelitian yang digunakan yaitu metode survei dengan pendekatan secara cross sectional dan teknik pengambilan sampel purposiveAnalisis data survei dilakukan dengan melihat positivitas DENV serotipe 3 pada nyamuk. Hasil penelitian menunjukkan DENVserotipe 3 tidak terdeteksi pada nyamuk yang di sampling di Purwokerto Timur. Kata kunci : Aedes aegypti, Demam Berdarah Dengue, DENV
Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java Rokhmani, Rokhmani; Wahyono, Daniel Joko; Mulyani, Lilis
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3924

Abstract

Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.
ENFORCEMENT OF ENVIRONMENTAL LAW ON WASTE MANAGEMENT AS A FORM OF IMPLEMENTING THE PRINCIPLES OF GOOD ENVIROMENTAL GOVERNANCE (GEG) BASED ON CHARACTER Elly Kristiani Purwendah; Daniel Joko Wahyono
Ganesha Law Review Vol 6 No 1 (2024): May
Publisher : Program Studi Ilmu Hukum, Jurusan Hukum dan Kewarganegaraan, Fakultas Hukum dan Ilmu Sosial, Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/glr.v6i1.3409

Abstract

This research aims to find out how environmental law enforcement regarding waste management is implemented as a form of implementing the principles of good environmental governance (GEG) based on character values. This research uses a normative juridical research method with a type of approach, namely a statutory approach and a conceptual approach. The legal materials used are primary, secondary and tertiary legal materials, obtained by conducting literature studies. The research results show that waste management is currently still an unresolved problem. There are several laws and regulations that have a correlation with waste management in Indonesia, namely Law no. 32 of 2009 concerning Environmental Protection and Management and several other laws. Law enforcement in waste management refers to 3 legal systems which are a combination of components, namely structure, substance and culture. Regulations regarding environmental law enforcement regarding waste must also apply character values, so that the goal of environmental law enforcement can be achieved, namely creating a sustainable environment in order to achieve a just, orderly, prosperous and characterized society. Apart from that, related to law enforcement in waste management, it can be studied from 2 sides, namely preventive and repressive law enforcement. Law enforcement in waste management is also an embodiment of the government and local governments in implementing the principles of Good Environmental Governance with the aim of raising public awareness of a good and healthy environment.
Relative Expression of mRNA BARF1 Epstein-Barr Virus from Tumor Tissue Biopsy in Formalin-Fixed Paraffin-Embedded in Nasopharyngeal Carcinoma Wahyono, Daniel Joko; Fuadi, Muflih; Susanto, Agus Hery; Sulistyo, Hidayat
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.413

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from nasopharyngeal epithelial cells. NPC is endemic in some regions, especially in Southeast Asia countries. NPC is also a multifactorial disease involving environmental factors, genetic factors, and infection from Epstein-Barr virus (EBV). According to WHO classification, Undifferentiated NPC is histopathologically associated with EBV infection and categorized into WHO subtype 3. EBV has two phases in its infection cycle: the lytic and latent phases. The BARF1 gene is a mediator of the transition from the latent phase to the lytic phase. Previous studies suggest measurement of EBV mRNA activity at the primary tumor site in the nasopharyngeal reflects the pathogenesis of NPC rather than measuring circulating EBV DNA or serological diagnosis. This study aimed to determine the relative expression potential of BARF1 mRNA at different tumor stages in NPC patients as a predictor of NPC pathogenesis. This research design was a descriptive research method in the form of a cross-sectional study. The samples used were 22 patients diagnosed as NPC WHO class III at the Anatomical Pathology Section of Prof. Dr. Margono Soekarjo, Purwokerto, who met the inclusion criteria. The relative expression of BARF1 mRNA was carried out using the one-step real-time RT-PCR technique and then calculated using a formula of 2-ΔCt. The T-test was used to compare the relative expression of early and late-stage BARF1 mRNA. The relative expression of BARF1 mRNA in the late-stage advanced stage (n = 6; 0.708292-0.840177; med = 0.7164655) was increased compared to the early stage (n = 2; 0.708841-0.712423; med = 0. 710632).
Co-Authors AGUS HERY SUSANTO Ahsanal Kasasiah Andreanne Poppy Estania Anton Budhi Darmawan Arie Ardiansyah Nugraha Ashar, Fadli Bambang hermani Damara, Dandy Satria Dewi Setia Triana, Ikama Dodi Safari Dodi Safari Dody Novrial, Dody Elly Kristiani Purwendah ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH Fitri, Safira Fuadi, Muflih Gumilas, Nur Signa Aini Haris Budi Widodo Hendro Pramono Hendro Pramono Hidayat Sulistyo Irfan Dwiandhono Jekmal Malau Korrie Salsabila Kusumaningsih, Oti Leader Alfason Lestari, Agatha Nabilla Lilis Mulyani Maretra Anindya Puspaningrum Meilani, Nanda Diva Miranti Oviani Nawangtantrini, Gita Nuniek Ina Ratnaningtyas Nur Signa Aini Gumilas Oedjijono Oedjijono, Oedjijono Oviani, Miranti Prayitno, Priskila Agnesia Priskila Agnesia Prayitno Pudyastiwi, Elisabet Purnomo Soeharso Reinhard Simbolon Rokhmani Rokhmani Rokhmani Rokhmani, Rokhmani Roni Afriansya Safari, Dodi Saryono Setianingsih, Tri Yuli Setyawati, Endang Ariyani Siwi Pramatama Mars Wijayanti Sulistiyawati, Indah Tri Yulia Ningsih Vivi Setiawaty Vivi Setiawaty Wahyu Siswandari Widodo Widodo Wisiva Tofriska Paramaiswari Zahra, Aliya Azkia Zahro, Aurora Fatimatuz