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The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Abstract

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups
The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Abstract

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups
The Cytotoxicity of Daruju Mangrove (Acanthus ilicifolius) Leaf Extract as Root Canal Irrigation Ratna Putri; Twi Agnita Cevanti; Henu Sumekar
Denta Journal Kedokteran Gigi Vol 12 No 1 (2018): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. Purpose: The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. Materials and Methods: The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml, 6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is > 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. Results: The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. Conclusions: Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.