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The Cytotoxicity of Daruju Mangrove (Acanthus ilicifolius) Leaf Extract as Root Canal Irrigation Ratna Putri; Twi Agnita Cevanti; Henu Sumekar
Denta Journal Kedokteran Gigi Vol 12 No 1 (2018): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. Purpose: The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. Materials and Methods: The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml, 6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is > 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. Results: The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. Conclusions: Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.
The effect of low viscous coir fiber resin composite immersion time on composition dissolution Wijaya, Yongki Hadinata; Cevanti, Twi Agnita; Soesilo, Diana; Rochyani, Linda
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.82-89

Abstract

Background: E glass fiber dental is a fiber that is often used in dentistry. The availability of E glass fiber in Indonesia is still very limited. Various types of non-dental glass fiber materials are found in the market as engineering materials with relatively cheap prices so that they are expected to be used as an alternative to E glass fiber dental, which one of is coconut fiber. The composition of non-dental glass fiber is almost the same as that of dental E glass fiber. The composition affects the mechanical properties and chemical properties of the fiber. Glass fiber compositions such as Na2O and K2O will increase water resistance. Method: This study was conducted using a post test only control group design. The material used in this study was a composite of coconut coir fiber. The subjects of the coco fiber composite were divided into 4 groups for solubility test based on ISO 4049. The results obtained were analyzed using one-way ANOVA (p = 0.000). The results showed the lowest mean component solubility (%) was in the coconut fiber composite group A (0.476±0.03) and the highest yield was in the coco coir fiber composite D (0.6±0.01). Results: The results of the one-way ANOVA test showed a significant difference in the coco fiber composite in the solubility of the components (p
PENINGKATAN KEBERSIHAN MULUT  MELALUI EDUKASI BERBASIS TOT BAGI GURU DAN ORANG TUA DALAM KEMITRAAN SEKOLAH Sarianoferni, Sarianoferni; Prameswari, Noengki; Hadinata, Yongki; Sumekar, Henu; Paramita, Ayulistya; Lukisari, Cane; Wedarti, Yoifah Rizka; Nafiah, Nafiah; Cevanti, Twi Agnita; Pargaputri, Agni Febrina; Saptaswari, Dianty; Ashrin, Meinar Nur; Hollanda, Ghita Hadi; Aprilia, Aprilia; Setyaningtyas, Dwi
BESIRU : Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2026): BESIRU : Jurnal Pengabdian Masyarakat, Januari 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/besiru.v3i1.2059

Abstract

Dental caries in children remains a significant public health problem in Indonesia, particularly among primary school-aged children. Limited oral health literacy and insufficient involvement of teachers and parents in oral hygiene guidance contribute to the high prevalence of dental caries. This study aimed to enhance children’s oral hygiene knowledge and practices through Training of Trainers (ToT)-based education for teachers and parents within a partnership between the Faculty of Dentistry, Hang Tuah University, and El Rahma Islamic Boarding School, Surabaya. A pretest–posttest design was applied, involving fifth-grade students as research subjects. Teachers and parents received ToT-based training delivered by dental professionals and subsequently provided oral health education to students. Knowledge levels were assessed using questionnaires administered before and after the intervention and analyzed using the Wilcoxon Signed-Rank Test. The results demonstrated a significant increase in students’ mean knowledge scores, from 58.14 ± 21.30 at pretest to 77.21 ± 18.30 at posttest (p < 0.05). In addition, partner satisfaction questionnaires indicated a very high level of satisfaction with the program implementation. In conclusion, ToT-based oral health education involving teachers and parents is effective in improving children’s oral hygiene knowledge and has the potential to promote sustainable oral health behavior changes within the school environment.
The Secondary Metabolites Screening and the Effectiveness (Ananas comosus (L.) Merr of the Queen Pineapple Stems in Decreasing the Number of Enterococcus faecalis’s Colonies Shinta Nurmaraya Febrianti; Twi Agnita Cevanti; Henu Sumekar
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: A cause of endodontic treatment failure was sterilization process error so that bacterias still left in the root canal. Enterococcus faecalis were resistant to sterilization medicines, it was necessary to develop natural sterilization medicines such as the Queenpineapple stems that had the antibacterial content. Purpose: The aim of this research was to determine the effectivity of the Queen pineapple (Ananas comosus (L.) Merr) stems extract in decreasing the Enterococcus faecalis’s colonies Materials and Methods: This research was true experimental with the post test only control group design. The Queen pineapple stems extract tested its secondary metabolites contents. Enterococcus faecalis ATCC 29212 cultured in Brain Heart Infusion Broth synchronized with Mc Farland 0,5 solution and divided into seven groups (n=7). It used dilution series method, each BHIB tube given by the 100% extract (P1); 50% (P2); 25% (P3); 12,5% (P4) ; 6,25% (P5) ; 3,125% (P6) and without the extract (K(-)), incubated for 24 hours and streaked on Mueller Hinton Agar and incubated for 24 hours, then calculate the colonies’ amount of each group. Datas analyzed by Mann-Whitney U-Test because only 50% and 25% concentration can be calculated. Result: The resultshowed that the Queen pineapple stems contained flavonoids, tannins, alkaloids, saponins, also there were significant differences among the 50% and 25% concentration groups p<0.05. Conclusion: The Queen pineapple stems extract’s 50% concentration was effective to decrease the number of Enterococcus faecalis’s colonies.
The effect of low viscous coir fiber resin composite immersion time on composition dissolution Wijaya, Yongki Hadinata; Cevanti, Twi Agnita; Soesilo, Diana; Rochyani, Linda
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.82-89

Abstract

Background: E glass fiber dental is a fiber that is often used in dentistry. The availability of E glass fiber in Indonesia is still very limited. Various types of non-dental glass fiber materials are found in the market as engineering materials with relatively cheap prices so that they are expected to be used as an alternative to E glass fiber dental, which one of is coconut fiber. The composition of non-dental glass fiber is almost the same as that of dental E glass fiber. The composition affects the mechanical properties and chemical properties of the fiber. Glass fiber compositions such as Na2O and K2O will increase water resistance. Method: This study was conducted using a post test only control group design. The material used in this study was a composite of coconut coir fiber. The subjects of the coco fiber composite were divided into 4 groups for solubility test based on ISO 4049. The results obtained were analyzed using one-way ANOVA (p = 0.000). The results showed the lowest mean component solubility (%) was in the coconut fiber composite group A (0.476±0.03) and the highest yield was in the coco coir fiber composite D (0.6±0.01). Results: The results of the one-way ANOVA test showed a significant difference in the coco fiber composite in the solubility of the components (p
Cinnamomum zeylanicum Essential Oil as an Antibacterial Agent and Dental Caries Prevention Rizal, Moh. Basroni; Cevanti, Twi Agnita; Revianti, Syamsulina; Azmanu, Aulia Alam
Denta Journal Kedokteran Gigi Vol 20 No 1 (2026): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v20i1.2

Abstract

Background: The prevalence of dental caries in Indonesia reaches 88.8%. Chlorhexidine, although effective, has side effects such as tooth discoloration. Cinnamomum zeylanicum is rich in cinnamaldehyde and eugenol, with antibacterial activity that makes it a natural alternative. Objective: To assess the effectiveness of Cinnamomum zeylanicum essential oil against Streptococcus mutans inoculated in Wistar rats (Rattus norvegicus) in preventing dental caries. Method: This study used 20 male Wistar rats, divided into five groups: negative control, positive control, and three treatment groups with Cinnamomum zeylanicum essential oil concentrations of 6.25%, 10%, and 12.5%. Streptococcus mutans (S. mutans) was inoculated orally in 0.2 ml using a pipette for three consecutive days. During the study, rats were fed a diet mixed with a sucrose solution (600 mg of sucrose dissolved in 1 liter of Aquadest) at a 1:500 ml-to-pellet ratio to induce caries. After 20 days of treatment, data were collected using a bacterial inhibitory zone assay by disk diffusion and salivary pH measurement. Data analysis was performed using one-way analysis of variance (ANOVA), Tamhane’s post-hoc test, and the Wilcoxon signed-rank test. Results: One-way ANOVA test showed no significant differences in the inhibitory zone (p>0.05). Post-hoc tests showed that all groups were not significant (p>0.05). The Wilcoxon signed-rank test showed significant differences in salivary pH before and after treatment (p<0.05). Conclusion: Cinnamomum zeylanicum essential oil has potential as an antibacterial agent and natural alternative in preventing dental caries against S. mutans, and is able to change the salivary pH to alkaline.