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Contact Name
I Gusti Ngurah Dwija Putra
Contact Email
dwijaputra07@gmail.com
Phone
+6285772042829
Journal Mail Official
meditoryjournal@gmail.com
Editorial Address
Jalan Sanitasi No 1 Denpasar Selatan
Location
Kota denpasar,
Bali
INDONESIA
Meditory : The Journal of Medical Laboratory
ISSN : 23381159     EISSN : 25491520     DOI : https://doi.org/10.33992/meditory
Core Subject : Science, Social,
Meditory : The Journal of Medical Laboratory secara khusus berfokus pada masalah utama dalam pengembangan ilmu di bidang laboratorium medis yang mencakup patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi.
Arjuna Subject : -
Articles 165 Documents
OVERVIEW OF BACTERIAL PATTERNS IN TABANAN GENERAL HOSPITAL Dewi, Sri Ratna; Diah witari, Diah; pradnyadari, Ayu; kahari, Siska
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.3097

Abstract

AbstractBackground: Resistant bacterial infections will affect the results of therapy, costs, spread of disease, and duration of illness. To minimize the losses incurred, it is necessary to monitor resistant bacterial and the use of antibiotics. Objective: To be able to identify, monitor, map and overcome antibiotic resistance and restrict bacterial transmission in hospitals, especially at the Tabanan General Hospital. Method: This research was conducted using a cross-sectional method using samples of inpatients and outpatients sent by clinicians to the Clinical Pathology Laboratory, Microbiology Sub Lab in period of January to December 2022. Results: Of the 590 positive culture samples in 2022, the majority were caused by Escherichia coli (15.25%) and 62.22% were ESBL. Apart from that, 8.31% were found to be Staphylococcus aureus and 53.06% were MRSA. Conclusion: The percentage of Escherichia coli bacteria that produce the ESBL enzyme is still high (62.22%). The percentage of Staphylococcus aureus bacteria that produce the MRSA enzyme is still high (53.06%). The antibiotics Meropenem, Amikacin, and Tigecycline are the antibiotics with the highest sensitivity to ESBL. The antibiotics Nitrofurantoin, Linezolid, and Quinupristin/Dalfopristin are the antibiotics with the highest sensitivity to MRSA. Keywords: bacterial patterns, description of bacterial distribution, antibiotic resistance
EXAMINATION FOR TYPHOID FEVER USING IMMUNO MAGNETIC BEADS INHIBITION (TUBEX TF) METHOD AT RSUD SITI FATIMAH SOUTH SUMATERA PROVINCE 2024 Meilani, Selly Asti
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3553

Abstract

Background: Typhoid fever was a systemic infectious disease caused by the Salmonella typhi bacteria. The symptoms included increased body temperature, headache, nausea, loss of appetite, weakness, diarrhea, liver and spleen damege and death. Usually occurs within 1 to 3 weeks after exposured to Salmonella typhi bacteria. Aims: This research aimed to find out how to check for typhoid fever using the Immuno Magnetic Beads Inhibition (Tubex TF) method at RSUD Siti Fatimah South Sumatra Province in 2024. Methods: The methodology used in this research was quantitative descriptive with a research sample of 4 serum samples from patients with suspected typhoid fever collected by purposive sampling. The results: The results showed one possitve sample with a score of 4 on Tubex TF and three negative samples with a score 2 on Tubex TF. Conclusion: This showed that the Tubex TF method can differentiate the severity of thyphoid fever effectively. 
ANALYSIS OF HEMATOLOGICAL PROFILES IN TUBERCULOSIS PATIENTS IN THE WORKING AREA OF THE KENDARI CITY HEALTH SERVICE Usman, Julianti Isma Sari; Hasan, Aswiro; musrifah, Sari; Fauzi, Ahmad Zil
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3037

Abstract

Background: Pulmonary tuberculosis is still one of the main health problems in the world. Treatment of TB cases is one of the main strategies for controlling TB because it can break the chain of transmission. Anti-tuberculosis drugs can kill and inhibit Mycobacterium tuberculosis bacteria, but the use of these drugs can have side effects on different organs, especially the hematological system. Aims: This study aims to determine the hematological profile of tuberculosis patients undergoing treatment. Methods: The type of research used is descriptive quantitative with a cross-sectional approach. The independent variable is pulmonary TB patients undergoing treatment, the dependent variable is the hematological profile with a sample of 70 tuberculosis patients whose specimens were taken in the form of venous blood with K3EDTA anticoagulant. The results: frequency of anemia in male TB 32 people and female 14 people, followed by a decrease in hemoglobin levels, hematocrit and erythrocyte count. The absolute value of the erythrocyte index in tuberculosis patients was found to be a normal MCV value (77%) and decreased by 23%. MCH values were normal in tuberculosis patients (89%) and 11% experienced a decrease. For MCHC and RDW CV, it was found that there was an increase in values above the reference values, however, TB patients (83%) had normal MCHC values and 17% had increased MCHC values. Likewise, 87% of RDW CV had normal RDW CV values and 13% of tuberculosis patients had increased RDW CV values. The erythrocyte sedimentation rate in TB patients increased in men by 63% and women by 50%. Conclusions: TB patients undergoing treatment have normal leukocyte and platelet counts. From the results of leukocyte examination, 85% of patients had normal leukocyte counts, 12% leukocytosis and 3% leukopenia. The number of platelets in tuberculosis patients is 93% normal, 1% thrombocytopenia and 6% thrombocytosis.Keywords: Hematology, Tuberculosis Patients, Pulmonary TB
ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA FROM GAMBUT TAPE Hanasia, Hanasia; Martani, Natalia Sri; Turnip, Oktaviani Naulita; Putri Welmart, Devina Vania; Puspita Sari, Dewi Marta
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4040

Abstract

Background: Lactic acid bacteria (LAB) are Gram-positive bacteria with probiotic properties, contributing to digestive health by balancing gut microflora. These bacteria are commonly found in fermented products, including gambut tape, a traditional Indonesian fermented food from Kalimantan. Gambut tape is known for its distinctive green color derived from katuk and pandan leaves.Objective:  This study aims to isolate and identify LAB from the gambut tape. Methods: LAB were isolated using Man, Rogosa, and Sharpe (MRS) agar and incubated at 37°C for 24 hours. Identification was performed through biochemical tests, including Gram staining, catalase, citrate, H2S, motility, indole, carbohydrate fermentation, methyl red, and Voges-Proskauer tests.Results:  Three isolates were obtained, exhibiting similar morphological features: milky white color, round shape, intact edges, convex elevation, and smooth surface. Biochemical results suggested that the isolates are identical to Lactococcus lactis, Lactobacillus plantarum, and Lactobacillus fermentum.Conclusions: The LAB isolated from the gambut tape shows potential as an antibacterial agent for disease prevention. Further research is needed to evaluate their antibacterial activity and characterize bacteriocins produced by these LAB.
CORRELATION BETWEEN BLOOD LEAD EXPOSURE, BLOOD PRESSURE, AND HEMOGLOBIN LEVELS AMONG ONLINE MOTORCYCLE TAXI WORKERS Prihatiningsih, Diah; Agus Ferry Sutrisna Putra, I Gusti Putu
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.2522

Abstract

Lead or Pb is a byproduct of fuel combustion in vehicle engines used as an anti-knock additive. Lead exposure can worsen the quality of air contaminated by motor vehicle emissions, which can affect the performance of online motorcycle taxi drivers. This study aims to assess the relationship between blood lead levels, blood pressure, and hemoglobin levels in online motorcycle taxi drivers in Dauh Puri Kelod Village. This study is an observational study using a cross-sectional design. The sample consisted of 30 respondents selected based on their length of work, age, and daily cigarette consumption. Data were collected through questionnaires, direct measurements, and lead examinations using the AAS method. The data were analyzed using Spearman rank correlation and multiple linear regression. The results showed a significant relationship between age and blood lead levels (p=0.000 and r=0.989); there was a significant relationship between length of work and blood lead levels (p=0.000 and r=0.683); there was a relationship between the amount of cigarettes consumed and blood lead levels, although the relationship was not very strong with a correlation of (p=0.038 and r=0.381); there was no significant relationship between blood lead levels and hemoglobin levels in online motorcycle taxi drivers (p=0.369 and r=-0.170); there was a significant relationship between blood lead levels and systolic blood pressure (p=0.000 and r=0.975) and diastolic blood pressure (p=0.000 and r=0.810). It is recommended to use personal protective equipment (masks) when working in gas stations and to regularly monitor health at least once a year.
IDENTIFICATION OF HUMAN HAIR USING DIRECT PCR TARGETING THE CYTOCHROME B GENE Tri Widodo, Wimbuh; Masjkur, Indah Nuraini; Huda, Qurrota A’yunil; Yudianto, Ahmad; Kristianto, Sonny; Putri, Rury Erina
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3784

Abstract

Background: Hair is one of the human parts that is easily scattered and easy to obtain for testing. Direct PCR which is PCR without going through the DNA extraction process has been widely used in various fields. The cytochrome b gene is one of the genes located in mitochondrial DNA. The gene is unique in that it has a small variation within one species organism but has a large variation between species. Aims: c. Methods: Hair was heated at 600C for 10 minutes then the sample was used for the PCR template. PCR was performed using the cytochrome b gene. The results: The analysis showed that human hair could still be detected on day 30 using direct PCR using the cytochrome b gene. Conclusions: This result will simplify and save time in analysis in various fields related to hair shafts.
THE EFFECT OF EDTA BLOOD STORAGE TIME AND ROOM TEMPERATURE ON THE EXAMINATION OF ERYTHROCYTE SEDIMENTATION RATE Nabila, Lazulpa; Durachim, Adang; Hayati, Eem; Noviar, Ganjar
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3517

Abstract

Background: Erythrocyte Sedimentation Rate (ESR) is an examination that aims to determine the speed of erythrocytes settling in the blood measured for 1 hour. The examination of ESR performed within 2 hours at most. However, the examination is often not performed immediately due to several reasons that cause delays for several hours. The room temperature during the examination affects the examination. The temperature in West Java has the highest average reaching 32°C. Aims: To determine an effect of EDTA blood storage time and room temperature on the examination of ESR. Method: This research is included in the type of quasi eksperimental research by involving variations in blood storage time immediately, 4 hours, and 5 hours and room temperature during examination is room temperature 20-25oC and temperature 29-32oC. Result: The result of this study showed that there was a significant effect of the variable EDTA blood storage time and room temperature on the examination of ESR which is indicated by the value of Sig. 0,00 (Sig. 0,05). This is because EDTA blood stored more than 2 hours after blood collection will affect the shape of the cells and the temperature can affect the settling speed. Conclusion: Based on the results of statistical tests, it can be concluded that there is an effect of EDTA blood storage time and room temperature on the examination of ESR
COMPARISON OF IMMEDIATE AND TWO-HOUR DELAYED BLOOD GLUCOSE RESULTS IN PREGNANT WOMEN Marini, Meta Nur'Izzu; Yansen, Aturut; Wijayanti, Dian Rachma
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.2911

Abstract

Background: Synthetic media for microbial growth are expensive, thus opening the way for making bacterial growth media using local raw materials at low prices. Jicama is one of the local raw materials that can be used as natural media for the growth of microorganisms. It contains a source of carbohydrates needed for the development of microorganisms. Aims: This study aims to determine the effect of variations in the concentration of Jicama, which has the potential to grow Staphylococcus aureus bacteria. Methods: This study used experimental research with the Static Group Comparison method. In this study, the experimental group Staphylococcus aureus was instilled in Jicama solid media with various concentrations (2%, 3%, 4%, 5%, 6%) with five repetitions. The results: The results showed that the average number of Staphylococcus aureus bacteria colonies in jicama flour with a concentration of 2% was 0, a concentration of 3% was 0, a concentration of 4% was 0.8, a concentration of 5% was 1.4, and at a concentration of 6% was 2.2. The results of the Kruskal-Wallis test with a degree of confidence of 0.05 obtained a p-value = 0.000 where the p-value 0.05 so that it can be concluded that there is a significant effect of variations in the concentration of jicama flour on the growth of Staphylococcus aureus bacteria. Conclusions: For future researchers, it is necessary to conduct further research using higher concentrations of jicama flour and by adding other nutrients, such as animal protein, so that the growth of Staphylococcus aureus bacteria is more optimal.
ISOLATION OF ESCHERICHIA COLI FROM PREGNANT WOMEN’S URINE AT BINANGA HEALTH CENTER MAMUJU Mahmud, Abbas; Haninggar, Rizki Dyah; Ahmady, Ahmady
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.3779

Abstract

Background: Urinary tract infections (UTIs) and asymptomatic bacteriuria during pregnancy can have detrimental effects on both maternal and fetal health. These conditions are associated with complications such as acute maternal illness, low birth weight, preeclampsia, preterm delivery, and intrauterine growth restriction. The causative agents of UTIs are Escherichia coli, Klebsiella sp, and Staphylococcus spp.Objective:  to isolate   and identify E. coli in the urine samples of pregnant womenMethods: This study employed a descriptive observational design. The research sample included pregnant women attending the Binanga Community Health Center in Mamuju. Urine was cultured in BHIB and incubated at 37°C for 24 hours. Subsequent bacterial isolation was performed by subculturing onto EMBA media and MacConkey agar. Colonies that developed were then analyzed using Gram staining and examined microscopically.Results: Of 25 sample urine samples from pregnant women, 25 were culture-positive on MacConkey Media and EMBA Media.  14 (56%) isolate colored bacteria metallic green on EMBA media that EMBA media is specifically for the growth for   E. coli bacteriaConclusions: 56% of the bacterial isolates from the urine samples of pregnant women were indicated as Escherichia coli, based on the presence of metallic green colonies observed on EMBA media
POTENTIAL COMBINATION OF VIRGIN COCONUT OIL (VCO) AND AZADIRACHTA INDICA EXTRACT FOR ACNE CONTROL: A COMPOSITION PHYTOCHEMICAL AND IN VIVO ANTIBACTERIAL EVALUATION karta, i wayan; Burhannuddin, Burhannuddin
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.3062

Abstract

Background: Treatment of acne can be done with topical and systematic therapy. There is the bacterial resistance to drugs, so other alternatives are needed that can utilize natural ingredients so that side effects can be reduced. Aims: The objective of this study was to determine the phytochemical content of a blend of VCO and Intaran (Azadirachta indica) leaf extract (VCO-I) at concentrations of 5%, 10%, and 20%, and to assess its effect on the in vivo inflammation induced by P. acnes. Methode: This study has a posttest-only control group design and is experimental. Rats in the experimental group were smeared with VCO-I at concentrations of 0% (P0), 5% (P1), 10% (P2), and 20% (P3); clindamycin (Cindala®) was used as the positive control (K+); and distilled water was used as the negative control (K-). The results: The results showed that VCO-I contained flavonoids, tannins, and alkaloids. The level increases as the mixture's concentration increases. Each VCO-I treatment was able to treat wounds or lesions that appeared due to the induction of P.Acne bacteria. There are differences in each treatment for healing the lesions. Based on the Post Hoc Bonferroni test P2 and P3 were not significantly different from K+, and K0 was significantly different from P0.. Conclusion: The addition of the extract can increase the activity of speeding up the healing of lesions and the effective mixture is at P2 and P3.