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Jurnal Medik Veteriner
Published by Universitas Airlangga
ISSN : 26157497     EISSN : 2581012X     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Veterinary
urnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry and animal welfare. Food animals, companion animals, equine medicine, aquatic animal, wild animals, herbal medicine, acupuncture, epidemiology, biomolecular, forensic, laboratory animals and animal models of human infections are considered. Jurnal Medik Veteriner (JMV) published two times a year: April and October.
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Articles 350 Documents
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Effects of Colostrum Probiotics on ACE-2 Expression and Hematology in Murine Models Immunized Against Canine Coronavirus Hamid, Iwan Sahrial; Solfaine, Rondius; Supriyadi, Supriyadi
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.343-349

Abstract

In modern society, the human-animal bond has significantly strengthened, with pets evolving into integral family members providing psychological support to their owners. In the current landscape of heightened focus on immune health and the widespread utilization of probiotic advancements globally, this study investigates the potential of colostrum probiotics to influence hematological profiles and ACE-2 expression in a murine model of canine coronavirus (CCV) infection. Twenty-four mice were categorized into four treatment groups: (C) placebo, (T1) CCV induction + isoprinosine, (T2) CCV induction + probiotic-based, and (T3) CCV induction + colostrum-based probiotics. CCV induction was performed via subcutaneous injection of a live-attenuated CCV vaccine at 60 mg/kg body weight for 7 consecutive days. Probiotics were administered via oral gavage for 14 consecutive days. On the 15th day post-treatment, euthanasia was performed, and blood samples were collected to examine hematological profiles and ACE-2 enzymes in the intestinal tissue sections. Data analysis, conducted using ANOVA followed by Duncan's test (p < 0.05), revealed a significant improvement in the T3 group, particularly in leukocytes, hemoglobin, lymphocytes, neutrophils, and ACE-2 expression. In conclusion, this study suggests that probiotics, specifically colostrum-based, enhance immune parameters and ACE-2 expression in the intestinal milieu of murine model subjected to CCV immunization.
Gastrointestinal Parasite Infections in Sheep from Torghondi, Islam Qala, and the Herat Slaughterhouse Tookhy, Nazir Ahmad; Bui, Dung Thi; Haidari, Esmatullah; Shakhes, Shoaib Ahmad; Nasiry, Zabihullah; Mohmand, Noor Ali; Pooyan, Abdul Qader; Haqmal, Mohammad Aman
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.253-261

Abstract

Gastrointestinal parasites (GIPs) pose significant health and productivity challenges to livestock, especially in regions with limited veterinary care and extensive farming practices. This study evaluated the prevalence of GIPs in sheep at the Torghondi and Islam Qala borders, as well as the Herat Slaughterhouse. A total of 450 faecal samples were examined microscopically, and 200 gastrointestinal tract samples (including stomach and intestinal contents) were analysed by dissection. The overall faecal infection rate was 77.3%, with prevalence rates of 76.7% at Torghondi, 90% at Islam Qala, and 65.3% at Herat Slaughterhouse. Eimeria spp. was the most prevalent parasite, detected in 73.7% of faecal samples. Nematodes (Nematodirus spp., Trichuris spp., Strongyle spp.) and trematodes (Amphistome spp.) were also identified. Gastrointestinal tract analysis revealed Trichuris spp. in 40.5% and Moniezia spp. in 37.5% of the samples. These findings highlight the roles of climate, livestock management, and environmental conditions on the distribution of GIPs. The high prevalence of parasites underlines the urgent need for effective control measures, including regular deworming, improved sanitation, and enhanced biosecurity practices. Future research should identify specific risk factors to inform targeted interventions and improve regional livestock health.
Osteogenic Differentiation Potential of Equine Dental Pulp vs. Periodontal Ligament Stem Cells: A Comparative In Vitro Study Purwaningrum, Medania; Haryanto, Aris; Kayanaveda, Yohanna; Sawangmake, Chenphop
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.262-272

Abstract

Equine mesenchymal stem cells (MSCs) are promising for bone tissue engineering (BTE) because of their capabilities of differentiating into osteoblasts. Cell therapy using equine MSCs has been introduced. Recently, dental-derived MSCs have gained significant attention due to their capabilities and ease of collection with minimally invasive collection methods. Dental stem cells show high plasticity, accessibility, and applicability for regenerative medicine and are thus considered alternative sources of MSCs. This study evaluated the characterization, osteogenic differentiation potential, and migration assay of equine dental pulp stem cells (eDPSCs) as compared with equine periodontal ligament stem cells (ePDLSCs). Equine dental stem cells from eDPSCs and ePDLSCs (n = 4) were isolated and expanded to passage 3. The morphology, colony-forming capability, cell proliferation assay, stemness and surface markers, trilineage differentiation potential, and migration assay were investigated in vitro. Both eDPSCs and ePDLSCs exhibited a fibroblast-like morphology; showed a colony-forming capability; were able to proliferate based on the results of the cell proliferation assay; expressed stemness and surface markers (NANOG, CD29, CD44, CD90, CD18); maintained the ability to differentiate into osteocytes, chondrocytes, and adipocytes and demonstrated migration capacity based on the migration assay. Surprisingly, ePDLSCs showed significant differences in matrix mineralization, quantification of Alizarin Red staining by cetylpyridinium chloride, and mRNA expression of the osteogenic marker RUNX2. ePDLSCs and eDPSCs may be better alternative MSCs than dental stem cells for the further design of therapeutic regimens for BTE and wound-healing therapy.
Fresh Semen Characteristics, Biochemical and Mineral Composition of Seminal Plasma from Different Age Groups of Balinese Cattle Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.273-285

Abstract

Age is among the factors affecting reproductive organs, particularly semen characteristics. Semen characteristics are also influenced by composition of seminal plasma that is ejaculated with the sperm. Therefore, this study aimed to investigate characteristics of fresh semen and seminal plasma composition from Balinese cattle aged 3, 8, and 13 years. Semen characteristics observed were concentration, progressive motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Biochemical composition of seminal plasma analyzed was fructose, malondialdehyde (MDA), total antioxidant capacity (TAC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), total protein (TP), albumin, cholesterol, and triglycerides. Mineral compositions observed were Na, K, Ca, Mg, Zn, Cu, Fe, and Se. The results showed that there were no significant differences (p > 0.05) in semen characteristics across age groups, meaning that semen of Balinese cattle can still be processed into frozen semen even at an older age, provided it meets the SNI 4869-1:2024 standards. Furthermore, the concentrations of each seminal plasma biochemical component were as follows: fructose (377.5–615.4 mg/dL), MDA (9.7–10.36 nmol/mL), TAC (66.22–67.22%), GOT (203–265 U/L), GPT (19–35 U/L), TP (4.3–5.5 g/dL), albumin (1.7–1.9 g/dL), cholesterol (11–35 mg/dL), and triglycerides (822–1.078 mg/dL). Surprisingly, the concentration of triglycerides in semen plasma in this study was very high compared to those reported in previous studies on several cattle breeds. Seminal plasma mineral concentrations included Na (1.500–1.900 mg/kg), K (600–700 mg/kg), Ca (600–800 mg/kg), Mg (38.85–45.16 mg/kg), Zn (5.33–9.64 mg/kg), Cu (0.06–0.12 mg/kg), Fe (11.85–15.88 mg/kg), and Se (0.92–1.48 mg/kg). These findings indicate that age did not affect semen characteristics, biochemical, and mineral composition of seminal plasma in Balinese cattle.
Modulatory Effect of Electroacupuncture on Isoflurane Anaesthesia in Cats Wahyuni, Sri; Soehartono, R Harry; Siswandi, Riki
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.297-307

Abstract

This study aimed to evaluate the combination of electroacupuncture (EA) and isoflurane as an anesthetic regimen in domestic cats. A total of 15 cats were divided into two groups, namely tiletamine-zolazepam (TZ) (n = 5) and EA (n = 10). Atropine was used as a premedication, injected 5 min before the induction of TZ. Approximately 10 min after induction, endotracheal intubation was applied, and cats were connected to isoflurane anesthesia. EA was applied to the EA group during the first 10 min of the total 30 min under isoflurane maintenance. Furthermore, the acupuncture points ST-36 and SP-6 were used at a frequency of 80 Hz and an intensity of 4 mA. The quality of anesthesia was evaluated by several parameters, including isoflurane requirements, anesthesia quality, cardiovascular function, and respiratory function. Data were collected every 10 min for a total of 30 min from the time of isoflurane application, followed by analysis using a t-test with Welch’s correction. The results showed that there was a significant reduction in isoflurane requirement in EA group, as indicated by the area under the curve (AUC) (p = 0.0003). Isoflurane concentration at 10 min was significantly lower in EA group (1.25 ± 0.71) than in TZ group (2.1 ± 0.22). It was also significantly lower at 20 min in EA group (1.05 ± 0.79) than in TZ group (1.8 ± 0.27). In conclusion, the addition of EA effectively reduced isoflurane requirements while maintaining a similar quality and safety of anesthesia in both groups.
Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice Tjahjono, Yudy; Wedharga, I Gede Putu Adhi; Novita, Bernadette Dian; Tahalele, Paul; Wijaya, Hendy; Soediono, Endang Isbandiati; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.308-317

Abstract

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.
Optimization of Primer Candidate Design for Toxocara cati Identification Using PCR Targeting the COX1 and ND5 Genes in Cats Yesica, Reza; Novita, Ni Kadek; Kusumarini R, Shelly; Sabri, Jasni
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.318-328

Abstract

Toxocara cati is an obligate extracellular parasite within the phylum Nematoda. This species is responsible for toxocariasis, a zoonotic disease. The disease transmission occurs via infective eggs, earthworms, cockroaches, birds, and rodents that contain larvae in their tissues. In cats, infection with this parasite can lead to symptoms such as diarrhea, hypoalbuminemia, anorexia, and a distended abdomen. This research aimed to identify an optimal candidate primer design through in silico analysis using BLAST primers from the National Center for Biotechnology Information (NCBI). This study targeted the mitochondrial genes COX1 and ND5 for the molecular identification of T. cati. Using in silico methods, primer candidates were designed and evaluated based on key parameters, including primer length, melting temperature (Tm), GC content, potential for secondary structure formation, and specificity. Candidate primers were screened using the NCBI primer-BLAST tool and validated through BLAST analysis to ensure sequence specificity. The primer pair that met all the criteria for an optimal candidate primer design comprised the forward primer TCND5F (5′-ACTGCTGGCCTTGTATTGGT-3′) and the reverse primer TCND5R (5′-ACACAGAACGCCTAAACCTCA-3′), both targeting the ND5 gene region.
Effects of Anchovy By-Product-Based Diets Supplemented with Soy Lecithin on the Reproductive Performance of Red Claw Crayfish (Cherax quadricarinatus) Majiun, Meccarren; Shapawi , Rossita; Yong, Annita Seok Kian; Tuzan, Audrey Daning; Lim, Leong Seng; Aznan, Alia Syafiqah
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.329-342

Abstract

The present study aimed to determine the growth and reproductive performances of red claw crayfish (Cherax quadricarinatus) fed formulated diets based on anchovy by-product meal, supplemented with varying levels of lecithin. The SL0 diet, containing 0% soy lecithin, was used as the control, and four experimental feeds were included with different lecithin levels: SL1: 1% soy lecithin, SL2: 2% soy lecithin, SL3: 3% soy lecithin and SL4: 4% soy lecithin. Five broodstock (four females and one male) were stocked in the square fiberglass tank (105 cm ´ 105 cm ´ 36 cm; 400L) and fed twice daily (0900h and 1600h) until satiation for 11 weeks. The data were analyzed using one-way ANOVA followed by Tukey’s test at p < 0.05. Growth and survival rates showed no significant differences among treatments. Notably, SL4 exhibited the highest gonadosomatic index (GSI) at 2.50 ± 1.99%, while SL3 had the highest hepatosomatic index (HSI) at 6.20 ± 1.18%. The spawning rate peaked in SL1 (63.89%), followed by SL0 (47.22%), SL2 (41.67%), SL3 (41.67%), and SL4 (25.00%). SL4 also had the highest fecundity (14.94 eggs/g female), with SL3 (10.72 eggs/g female) and SL0 (9.50 eggs/g female) following. Lipid content in muscle varied from 1.14% (SL4) to 1.46% (SL3), with no significant differences. However, lecithin inclusion notably affected lipid content in hepatopancreas and gonad, with SL1 exhibiting the highest hepatopancreas lipid (61.04%) and SL2 the highest gonad lipid (27.78%). The data suggest that dietary lecithin plays an important role in the reproductive performance of red claw crayfish. Overall, anchovy by-product meal has high potential to serve as a dietary ingredient in the feed formulation for both juvenile and broodstock of red claw crayfish.
Dietary Chlorella vulgaris Improves Growth and Modulates Gut Microbiota in Giant Freshwater Prawn (Macrobrachium rosenbergii) Norazman, Ammar; Tahir, Nur Diyana Mohamad; Salleh, Annas; Nor, Norhariani Mohd; Azmai, Mohamad Noor Amal; Ikhsan, Natrah
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.350-367

Abstract

The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially vital species in global aquaculture due to its rapid growth and high market demand, and Chlorella vulgaris is a nutrient-rich microalga lauded for its potential as a sustainable feed supplement. In this preliminary study, the effects of C. vulgaris supplementation on the growth and gut microbiota of giant freshwater prawn was investigated. Prawns were randomly assigned to three groups and fed diets supplemented with C. vulgaris at 0 g/kg (control), 30 g/kg (T1), or 60 g/kg (T2) for 14 days. For growth performance analysis, body weight was measured in a randomly selected 10% of the population at the beginning, midpoint, and end of the experiment. For gut microbiota analysis, faecal samples were taken from M. rosenbergii and used for DNA extraction and amplification via PCR. The PCR product was sequenced using 16S rRNA sequencing on Illumina platform. Results showed significantly improved growth rates in the supplemented groups, with T2 (highest growth rate) having 55% higher weight gain compared to the controls. Gut microbiota analysis revealed increased diversity, with the alpha biodiversity indices showing higher indices in supplemented groups compared to the controls although non-significant (p = 0.196, 0.136, 0.532 and 0.304 for Ace Chao1, Shannon and Simpson respectively). Specifically, the T1 group (highest alpha biodiversity) exhibited the following increases compared to the control: Ace by 53.55%, Shannon by 61.16%, Chao1 by 55.94%, and Simpson by 55.90%. In β-biodiversity analysis, there were shifts in bacterial community composition, with a notable increase in beneficial bacteria such as Synechocystis spp. and decrease in potentially pathogenic ones such as Chitinibacter spp. in the supplemented groups. PICRUSt2 analysis predicted enhanced metabolic pathways related to vitamin synthesis and energy metabolism, suggesting improved gut health and nutrient utilization. Although some results lacked statistical significance due to the short study duration, the findings suggest that long-term C. vulgaris supplementation may yield more pronounced benefits in M. rosenbergii aquaculture, potentially improving growth performance.

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