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Jurnal Medik Veteriner
Published by Universitas Airlangga
ISSN : 26157497     EISSN : 2581012X     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Veterinary
urnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry and animal welfare. Food animals, companion animals, equine medicine, aquatic animal, wild animals, herbal medicine, acupuncture, epidemiology, biomolecular, forensic, laboratory animals and animal models of human infections are considered. Jurnal Medik Veteriner (JMV) published two times a year: April and October.
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Articles 350 Documents
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Post-Natal Indonesian Garut Sheep Performance Fed with Sorghum-Indigofera-Mixed Feed and Stimulated with Pregnant Mare Serum Gonadotropin Hormone Somanjaya, Rachmat; Fuah, Asnath Maria; Rahayu, Sri; Abdullah, Luki; Setiadi, Mohamad Agus
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.63-73

Abstract

This study aimed to determine the effect of sorghum-indigofera (SI) mixed feed and pregnant mare serum gonadotropin (PMSG) hormone stimulation on ewe’s reproductive performance and Garut lamb’s pre-weaning growth. Twenty-eight multiparous Garut ewes were included into four treatment groups (2 ´ 2) with a factorial-Completely Randomized Design (CRD). The first factors were the types of feed local forage (LF) and SI, while the second factor was PMSG hormone stimulations (with and without PMSG stimulation). Synchronized estrous used twice injections with PGF2α, 11 days apart, and 500 IU PMSG was adminstrated at the second PGF2α injection intramuscularly. The observed variables included ewes' performance after parturition and post-natal lamb's growth. The obtained data were analyzed by multivarians of analysis (MANOVA) with α = 0.05 and continued with Tukey's Test. The result showed that feeding ewes with SI without PMSG stimulation resulted in the highest number of pregnancies, litter size, and lactation compared to other treatments. Feed type and PMSG stimulation factors has no any interaction effect (p > 0.05) on birth weight, weaning weight, and milk production. However, birth weight and milk production were affected (p < 0.05) by the feed, while weaning weight was affected (p < 0.05) by the PMSG stimulation. The fastest lamb growth rate was found in the groups of ewes fed by SI without PMSG stimulation (120 g/head/day). It can be concluded that SI could improve the ewe’s reproductive performance and the post-natal growth of Garut lambs. Meanwhile, the PMSG hormone could beneficially with sorghum-indigofera mixed feed.
Phenotypic and Genotypic Detection of Staphylococcus aureus and Escherichia coli in Subclinical Mastitis Goat Milk in Lumajang, Indonesia Khasanah, Himmatul; Putra, Nalendra Gigih Wibawanto; Hariyadi, Nanang Tri; Widodo, Nur; Widianingrum, Desy; Yulianto, Roni; Cruz, Joseph F. Dela; Pratiwi, Nurul
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.167-174

Abstract

This study aimed to identify the Staphylococcus aureus and Escherichia coli bacteria that cause subclinical mastitis in dairy goats in the Senduro sub-district of Lumajang regency, Indonesia. A total of 116 milk samples from 58 dairy goats were tested for the California Mastitis Test (CMT). CMT-positive samples were used for bacterial identification through phenotypic and genotypic analyses. Phenotypic analysis was conducted by culturing bacteria on natrium agar media and followed in selective media. The genotypic analysis employed a specific primer of S. aureus using the 23SrRNA gene and E. coli using the 16SrRNA gene as confirmation of the gold standart. The results of this study showed positive subclinical mastitis in dairy goat samples, which consisted of 21 samples of CMT 1 and a sample of CMT 2. Bacterial identification with phenotypic assays documented 16 samples having S. aureus and one sample having E. coli. The validated genotypic assays showed from 16 samples, validated 5 samples of S. aureus infection and one E. coli infection. In conclusion, bacterial identification of S. aureus and E. coli predominantly infects the SCM dairy goats in the Senduro sub-district as evidenced by the phenotypic analysis and followed by genotypic using the PCR method.
Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression Shabira, Zahra; Widjiati, Widjiati; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Safitri, Erma; Mafruchati, Maslichah; Hendrawan, Viski Fitri
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.175-182

Abstract

This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Sweet Potato Leaf Extract as a Protective Antioxidant: Improving Hematological Health in Ammonia-Exposed Mahseer Fish Anggreani, Shovia Finny; Imlani, Ainulyakin Hasan; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono, Suciyono
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.89-98

Abstract

The Mahseer fish (Neolissochilus soroides) is a highly valuable aquaculture species due to its economic potential. Currently, this species is being developed in intensive aquaculture systems. As a result, ammonia levels are increasing, which could potentially disrupt the growth and survival of the fish. This study aimed to investigate the effects of sweet potato (Ipomoea batatas) leaf extract supplementation as an antioxidant on the hematological profile of Mahseer fish exposed to NH4Cl. Four supplementation treatments were employed: 0%, 2.5%, 5%, and 7.5%, each with five replicates. The fish were supplemented with the extract for 40 days and exposed 10 ppm NH4Cl for 48 hours. Blood samples were collRected before rearing, after 40 days of supplementation and NH4Cl exposure. The results demonstrated that supplementation with sweet potato leaf extract (SPLE) positively influenced the hematological profile of mahseer. Specifically, higher doses of the extract enhanced immunity across all treatments. Notably, only the highest doses of 7.5% and 5% effectively mitigated fish stress induced by ammonia exposure for 24 hours. Furthermore, there were no significant differences observed among treatments in response to NH4Cl exposure throughout the study period. These findings underscore the potential of SPLE as an antioxidant supplement to bolster immune function and alleviate oxidative stress in mahseer under ammonia exposure conditions. Further research could focus on optimizing the dosage of SPLE to maximize its antioxidant benefits in aquaculture setting.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
First Record of Anguillid Herpesvirus 1 Linked to a Mass Mortality Event in Shortfin Eel (Anguilla bicolor) in Indonesia Romadhona, Ekky Ilham; Widantara, Handang; Aslia, Aslia; Megawati, Novi; Ardiansyah, Arif Rahmat; Larassagita, Annisa Fitri; Dewi, Kiki Mariya; Farman, Aditia; Chaidir, Iding; Sujatmiko, Wisnu; Yaniharto, Dedy; Budiardi, Tatag; Aliah, Ratu Siti; Sutanti, Sutanti
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.134-143

Abstract

Anguillid herpesvirus 1 (AngHV-1), a member of the Alloherpesviridae family, is known to cause high mortality in both wild and farmed eels. Notably, no cases of AngHV-1 infection in Indonesia until June 2023, when a significant mortality rate exceeding 75% among cultured glass eels was documented in Bogor, Indonesia. This study investigated the outbreak by collecting 30 diseased fish from multiple cultured tanks to examine clinical symptoms, histopathological changes, and viral presence through PCR targeting the viral DNA polymerase gene. Hemorrhagic lesions in the abdomen and anal regions were the primary clinical symptoms. Histopathological examination revealed hyperplasia, fusion, and epithelial lifting of the gill secondary lamellae. PCR, using 394 bp primer specific for AngHV-1, confirmed 100% infection among the collected samples, indicating rapid viral transmission within the rearing environment. Phylogenetic analysis of partial DNA polymerase amino acid sequences showed that Indonesian AngHV-1 isolate is genetically diverse and shares similarities with strains from China, Taiwan, Canada, and several European countries, suggesting the emergence of a novel strain. This study highlights the urgent need for enhanced biosecurity measures to curb AngHV-1 spread in the Indonesian eel aquaculture sector.
Genomic Characterization of Vibrio spp. in Asian Seabass (Lates calcarifer, Bloch, 1790) Following Field Vaccination Using a Feed-Based Inactivated Vaccine against Vibriosis Tahir, Nur Diyana Mohamad; Yap, Sing Yee; Nor, Norhariani Mohd.; Yasin, Ina-Salwany Md.; Azmai, Mohammad Noor Amal; Zahli, Nurul Izzati Uda; Gan, Han Ming
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.99-113

Abstract

Vibriosis outbreaks pose a significant threat to the productivity of Asian seabass culture, causing substantial losses. Nevertheless, the excessive utilization of antimicrobials exacerbates the issue by fostering the development of antimicrobial resistance (AMR). Consequently, exploring alternative disease management strategies, such as the introduction of oral vaccines into Asian seabass culture, has become a subject of ongoing investigation. This study aims to compare the genomic characteristics of different Vibrio species isolated from both orally vaccinated and unvaccinated Asian seabass populations. Archived samples of vaccinated and unvaccinated Asian seabass from one site in Selangor, Malaysia, were utilized in this sample. Briefly, the vaccinated group was administered the feed-based vaccine on week 0 (prime vaccination), 2 (booster), and 6 (second booster) at 4% body weight. At the same time, the non-vaccinated fish were fed with a commercially formulated pellet without the vaccine. Vibrio isolates identified from the gut samples were used in this study. The samples were stored at -80°C before being subjected to genomic DNA extraction, PCR, gel electrophoresis, and sequencing using Illumina and Nanopore platforms. Universal 16s primer and pyrH primer were used to identify Vibrio species. Bioinformatic analysis was done using NCBI BLAST, QUAST, BUSCO 5, CGE, and J Species. The isolates of Vibrio species exhibited smooth, convex, round, and entire colonies on TCBS agar plates, which were yellow and green. Twenty-two isolates were sent for 16s rRNA sequencing and revealed Vibrio alginolyticus (54.54%), followed by V. diabolicus (13.63%) and V. parahaemolyticus and V. harveyi (9.09% respectively). Of the 22 samples, 7 were selected for further Illumina sequencing. The whole genome sequences of the six Vibrio species isolated exhibited good coverage percentage, N50 value, Average Nucleotide Identity (ANIb), single-copy percentage, and GC content, while one sample showed low single-copy percentage and high duplicated percentage, which suggested contamination during DNA extraction. Eight novel alleles were discovered, three from the vaccinated group and five from the unvaccinated group, including the Rec, atpA, gyrB, and pyrH. A virulence factor database analysis search revealed 58 virulent genes from the unvaccinated samples and 39 virulent genes from the vaccinated samples. Overall, this research provides valuable insights into the genomic characteristics between orally vaccinated and unvaccinated cultured Asian seabass in the locality.
Update of Hulse´s Technique for the Surgical Correction of Cranial Cruciate Ligament Rupture and Its Long-Term Postoperative Evaluation in a Group of Canines de Aurrecoechea, Claudia; Pinhas, Álvaro
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.183-198

Abstract

Rupture of the cranial cruciate ligament is the oldest and most prevalent cause of osteoarthrosis (OA) of the patellofemoral joint (PFJ) in canines, being 75% of all PFJ surgical pathologies. To assess the long term clinical, radiological and orthopaedic results of a modification of Hulse’s technique for intracapsular surgical correction of cranial cruciate ligament rupture (CrCLR). A heterogeneous population of 12 canines who had undergone surgery for CrCLR was called for the study, of which 3 underwent surgery on both PFJs. The technique was modified by using a pre folded crochet needle and a tiny lateral arthrotomy performed just under the lateral collateral ligament. This study was done after 20 months on average from de surgery (range 8 to 36 months) and all the surgeries were performed by the same surgeon. All animals were monitored during the study period, which lasted 4 months. A blind radiological assessment of 3 observers was performed using the Bioarth scale, an orthopaedic assessment to quantify the degree of claudication and PFJ angles (goniometry) and two scales were also used for the assessment of chronic pain and animal welfare i.e., Helsinki's scale and CPBI (canine pain brief inventory). The radiographic assessment of OA yielded a mean of 9.13, with SD of 5.50 and a SE of 1.42. The average subjective assessment of OA according to the Bioarth scale was moderate. The qualitative orthopedic assessment showed that 7 patients did not present claudication, 4 patients showed grade 1 claudication and one grade 2. The CPBI and Helsinki animal welfare scales showed an excellent subjective assessment by the tutors. The Hulse technique, modified with a crochet needle, was sufficient to surgically correct the CrCLR in an heterogeneous population of 12 canines, achieving that all patients functionally used the operated limb or limbs, possessing a very good to excellent quality of life according to their owners, despite having evidenced a moderate degree of OA with an average of 9.13 on the Bioarth scale.

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