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YOHANES BARE
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JL. KESEHATAN, NO 03, KELURAHAN BERU, KECAMATAN ALOK TIMUR, KABUPATEN SIKKA, PROVINSI NUSA TENGGARA TIMUR 86111
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Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Published by Universitas Nusa Nipa
ISSN : 2716151X     EISSN : 2722869X     DOI : http://dx.doi.org/10.55241/spibio
Jurnal ini fokus pada bidang kajian biologi sains dan pendidikan biologi. Kami menerima artikel berupa hasil penelitian maupun kajian pustaka yang original dan belum pernah dipublikasi.
Arjuna Subject : Umum - Umum
Articles 180 Documents
Uji Resistensi Bakteri Endofit dari Akar Eceng Gondok (Eichhornia crassipes) terhadap Linear Alkylbenzene Sulfonate Arini Nurul Mahmudah; Agustina, Eva; Faizah, Hanik
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.478

Abstract

Linear Alkylbenzene Sulfonate (LAS) is an anionic surfactant with the potential to cause environmental pollution. One effective bioremediation strategy to mitigate this pollution is the use of endophytic bacteria. This study aims to evaluate the resistance ability of endophytic bacteria isolated from the roots of water hyacinth against LAS. The research was conducted as a laboratory-based experimental study using a Completely Randomized Design with five replications for each isolated endophytic bacterium. Bacterial resistance to LAS was tested in a liquid medium containing 10 ppm LAS and incubated for 14 days. The resistance level was determined based on the bacterial optical density (OD) measured using UV-VIS spectrophotometry. The results identified four endophytic bacterial isolates belonging to the genera Bacillus, Streptomyces, Micrococcus, and Neisseria, all of which exhibited resistance to LAS. The highest resistance level was observed in Streptomyces with an average OD value of 0.728 ± 0.11, while the lowest resistance was found in Bacillus with an average OD value of 0.361 ± 0.04.
Keanekaragaman Gastropoda di Pantai Muko Wujo Kecamatan Larantuka Kabupaten Flores Timur Malik, Margaretha; Laynurak, Yoseph M.; Semiun, Chatarina Gradict
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.480

Abstract

Gastropods act as environmental bioindicators because they are sensitive to changes in the aquatic environment. The purpose of this study was to determine the diversity of gastropod species on Muko Wujo Beach. Determination of sampling points using the purposive sampling method. Samples were taken at two different stations based on human activities. The stages in collecting data on gastropod species include observing gastropods found in a 1x1 m plot, counting the number, and then recording it for identification purposes. In addition, environmental parameters, namely temperature and pH, were measured. Data on gastropod species and environmental parameters were then analysed qualitatively and quantitatively. Qualitative analysis was carried out by describing the morphological characteristics of gastropods, and identification was carried out using the help of scientific articles and internet websites. Quantitative analysis was carried out by calculating the diversity index, dominance index and similarity index of gastropod species. The results showed that 29 types of gastropods were found on Muko Beach, Larantuka District, East Flores Regency, which were divided into 14 families and 7 Orders. The diversity index, dominance index, and similarity index of gastropod species on Muko Wujo Beach were classified as moderate.
Efektivitas Pistia stratiotes dan Salvinia molesta sebagai Agen Fitoremediasi Limbah Sungai Ciliwung Aulya, Nailul Rahmi; Ristanto, Rizhal Hendi; Nurazizah, Meiza; Febryaningrum, Sukma; Muthiah, Anis; Fauziyah, Fina
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.484

Abstract

Rapid development and increasing population growth affect river water quality, including the Ciliwung River. The decline in the water quality of the Ciliwung River is caused by organic waste originating from households, industry, livestock, and agriculture, so efforts are being made to manage the water using phytoremediation. Pistia stratiotes and Salvinia molesta are aquatic plants that can absorb heavy metals in polluted water. This research aims to determine the effectiveness of plants Pistia stratiotes and Salvinia molesta in reducing the Ciliwung River's wastewater level. This type of research is laboratory-scale experimental with RAL in 3 treatments (A, B, and C) and 6 replications. Data were analyzed using the ANOVA test with the DMRT further test. The results of the research showed that the treatment of two types of plants had a significant effect on reducing the level of water turbidity with the greatest reduction rate being found in plants Salvinia molesta. The fastest change in color and reduction in the number of leaves occurs in plants Pistia stratiotes, and the levels of AAS contaminants before and after treatment cannot be detected because the detection limit of the smallest limit test parameter possessed by a tool/instrument for measuring a certain number of analytes is different.
Studi Etnobotani Tumbuhan Obat di Kelurahan Pendahara Kecamatan Tewang Sangalang Garing Kabupaten Katingan Araina, Elga; Rahmi, Ennike Gusti; Neneng, Liswara; Basuki, Bejo; Wijaya, Nuriman; Akhmadi, Akhmadi; Fahrina, Ririn; Adam, Chaidir; Panny, Priscilla; Herniati , Melly
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.491

Abstract

The use of plants by a part of society in the cultural scope is studied in a special branch of biological science, namely ethnobotany. Ethnobotany is a science that studies the relationship between humans and plants and their environment based on the life experienced by society. This study aims to find out what types of plants are used as medicine by the local community in Pendahara Village and to discover how to process and use plants as medicine by the community. The method in this study employs an exploratory survey method, which is research that seeks to explore something new, not widely known by the public, and aims to be studied more deeply by conducting direct interviews with sources and informants, namely the community in Pendahara Village. Data collection techniques are carried out by providing interviewers, observations, interviews and documentation. The results of the study found 16 types of plants that can be used as traditional medicine. The results of the study found 16 types of plants that can be used as traditional medicine. These plant species are Orthosiphon aristatus, petindis plants, Hippobroma longifora, Tinospora cordifolia, Arcangesia flava (L.) Merr, Annona muricate, Hibiscus rosa-sinensis L., Asplenium nidus L., Averrhoa bilimbi L., Areca catechu, Psidium guajava L., Cordyline, Acorus calamus L., Phyllanthus niruri L., Kalanchoe pinnata, and Luvunga sarmentosa. It was concluded that the use of the plant as a traditional medicine has been felt to be effective in external and internal medicine.
Pengaruh Penerapan Model PAIKEM dipadu Media Mind Map pada Materi Sistem Ekskresi terhadap Hasil Belajar Peserta Didik Kelas XI di SMAN 1 Lambu Kabupaten Bima Arbiah, Muslimah; Hasanah, Ummul; Maulana, Andi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.498

Abstract

Education plays a role in the quality of life; therefore, education must create enjoyable learning. A good learning mode is really needed to support active, effective, creative innovative and fun learning. The PAIKEM model is a learning model that reduces unpleasant learning to become enjoyable. The research aims to determine the affective of applying the PAIKEM model combined with mind map media on excretory system material on the learning outcomes of class XI MIA students at SMAN 1 Lambu, BIMA regency. The research population was class XI MIA at SMAN I Lambu, totaling 90 people. The sample consisted of 62 students, using purposive sampling. The instrument used were learning outcomes tests, observation and interviews. The analysis techniques used are descriptive statistical analysis and inferential statistical analysis research, a sig of 0,000 was obtained, indicating that sig a = 0,000 < 0,05, so that H 0 was rejected and H1 was accepted. The results show that there is a significant influence from the application of the PAIKEM model combined with mind map media in the excretion system material on the learning outcomes of class XI MIA students at SMAN 1 Lambu, Bima Regency. The implication of this research is that the PAIKEM model can be applied to increase creative, innovative and enjoyable learning. 
Peralihan dan Regenerasi Pengetahuan Beserta Skema Kebijakan Retensi Etnobotani Pewarna Alami pada Masyarakat Blok Kebon Gedang Cirebon Muhammad Abdul Jabar; Iryani, Riris; Nadifa, Jasmine Augi; Widiatningrum, Talitha
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.506

Abstract

Kebon Gedang Block is one of the few areas of natural dye batik craftsmen in Indonesia. The activity of using natural dyes for batik has been carried out from generation to generation. This study aims to analyze the transfer of ethnobotanical knowledge of natural dyes based on age class (KU), analyze ways to regenerate and analyze the policy scheme for retention of ethnobotanical knowledge of natural dyes in the Kebon Gedang Block. The main methods used in this study were semi-open interviews, observation, and documentation. The selection of respondents was carried out by purposive sampling by considering age (years), namely I (30-39), II (40-49), III (50-59), IV (> 60). The results showed that the level of knowledge of natural dyes and retention was highest in KU III because the people were still healthy, strong, and generally still actively following the development of the batik industry. However, KU III is the group with the highest change in knowledge and is feared to be lost. While at other KU levels, the inheritance of knowledge is still going well. The policy of retaining ethnobotanical knowledge of natural dyes can be carried out in several ways, including by creating books, booklet or electronic sites about knowledge of natural dyes, as well as by registering intellectual property rights. This research can record the current state of knowledge so that efforts can be made to avoid losing this knowledge. 
Uji Bioaktivitas Ekstrak Daun Sirih Keraton (Cissus discolor) sebagai Antioksidan dan Antibakteri terhadap Escherichia coli Salim, Apriliani Eka Syakira; Afdhalia, Sinar; Azikra, Nurul; Bau, Andi Besse Tenri; Marzukah, Marzukah
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.518

Abstract

This study aims to explore and identify the potential of betel palace leaves (Cissus discolor) as a natural antibiotic candidate in overcoming increasing bacterial resistance. Conventional antibiotic resistance has become a serious problem in the treatment of various diseases caused by pathogenic bacteria such as Escherichia coli. Betel palace leaf is one of the traditional plants believed to have antibacterial and antioxidant properties. However, it has not been widely explored scientifically. The research method used was a mixed method involving exploratory and experimental approaches. Exploration was conducted to identify the morphology and characteristics of the betel palace plant. The research design used a completely randomized design (CRD) with three variations of extract concentration, namely 50%, 75%, and 100%, as well as positive control (chloramphenicol) and negative control (distilled water). Antibacterial test was conducted against E. coli bacteria using the disc diffusion method. Antioxidant test with DPPH method was tested in various concentrations of 10%, 20%, 50%, 100%, 150%. Phytochemical screening test was used to identify bioactive compounds in the extract. The results showed that the keraton betel leaf extract had weak to moderate antibacterial activity with the largest zone of inhibition obtained at 100% extract concentration. This research contributes to the development of herbal medicine by providing new scientific data related to the potential of Cissus discolor as a source of antibacterial compounds and antioxidants.
Minat Belajar Peserta Didik terhadap Pembelajaran Biologi Menggunakan Metode Praktikum pada Materi Transpor Membran Sel Adillah, Dzuhrotin; Jamaludin, Didi Nur; Fikri, Achmad Ali; Setyawan, David Ari
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.529

Abstract

Biology learning is often dominated by a theoretical approach because many theories cause students to feel bored and less interested. The research aims to determine the learning interest of MA Mazro’atul Huda Karanganyar Demak students towards learning biology using practical methods of cell membrane transport material. This type of research uses quantitative descriptive methods. The research subjects were 26 students of class XI MIPA. The data collection technique uses an instrument in the form of a questionnaire which aims to obtain information regarding students’ learning interests. The research data were analyzed by calculating student questionnaire scores, then converted into percentages and interpreted into categories of very good, good, poor, and not good. The research results showed that 74% of students had a fairly good interest in learning while following the biology learning process using the practicum method. The indicator of learning interest with the highest average value is student involvement in biology subjects, namely 81% with a very good category because students are directly involved in the practicum process to work together in organizing, communicating, and interpreting practicum results. The implication is that in biology learning, specifically cell membrane transport material, teachers should use practical methods because it can foster students’ interest in learning.
Kualitas Bakteriologi (Fecal Coliform) Mata Air Desa Toobaun Kabupaten Kupang Sarifan, Gracyana Putri; Semiun, Chatarina Gradict; Mamulak, Yulita Iryani
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.530

Abstract

Springs are groundwater that comes out on its own to the surface of the earth. Water sources originating from springs are generally suitable for direct consumption because they undergo natural purification. However, the high demand for clean water causes springs to experience anthropogenic pressure, which can reduce the quality of spring water. The study aimed to determine the Bacteriological quality of spring water in Toobaun village. The method used in this study was the Most Probable Number (MPN), using a series of five tubes with a variety of 5-5-5. The test included three stages, namely the prediction test, the confirmation test, and the complete test. The data obtained were presented in the form of a table, then compared with the MPN table value and analyzed descriptively by comparing the MPN value with the water quality standard, namely PP No. 22 of 2021 (class I). The results of the study showed that the Fecal Coliform value found was 220 MPN/100ml and did not meet the quality standards of PP No. 22 of 2021 (Class I). In the complete test using EMBA media, three types of bacteria were found that had characteristics similar to Escherichia coli and Salmonella sp. Klebsiella sp. Based on the results of water testing at the Toobaun spring, it cannot be consumed directly without first being processed.
Tinjauan Literatur: Penerapan Model Pembelajaran Berbasis Proyek untuk Meningkatkan Kemampuan Berpikir Kreatif Siswa pada Pembelajaran Biologi SMA Miranda, Sonya; Hardinah, Nur Ayu; Melisa, Atika Okta
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.533

Abstract

Creative thinking ability is one of the essential competencies of the 21st century that needs to be instilled in biology learning. however, many studies show that conventional approaches have not been effective in developing students’ creative thinking skills. This study aims to preliminary research data on project-based learning models in improving students’ creative thinking skills in biology learning through a literature review. The research method used a literature study with the help of prism in analyzing 12 relevant scientific articles, published between 2020 and 2025, from various scientific journals/articles, and books. The analysis was conducted based on the implementation, effectiveness, and impact of project-based learning models on creative thinking skills. The results of the review show that the project-based learning model is able to significantly improve students’ creative thinking skills through activities that involve exploration, problem analysis, and application of biological concepts relevant to real life. In addition, the project-based learning model is also be able to encourage collaborative and develop communication skills. The application of project-based learning model is very effective in learning because it provides meaningful and relevant learning experiences by involving students directly in learning. The conclusions obtained from the 12 articles analyzed provide the same statement and evidence that the application of project-based learning models has significant potential in improving creative thinking skills in biology learning.