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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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Effect of Synthetic Fertilizer on Diazotrophic Bacteria in Secondary Forest and Oil Palm Soils in Central Kalimantan Agustina, Delia; Purnamasari, Maria Indah; Karmawan, Listya Utami; Suwanto, Antonius
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.106-116

Abstract

Soil microorganisms, especially diazotrophic bacteria, are vital for ecosystem sustainability, significantly contributing to nitrogen cycling and biodiversity conservation. Understanding the impact of different land-use practices on soil microorganisms, especially synthetic fertilizer addition, is essential for sustainable agriculture. This study compares soil properties, bacterial densities, and responses to fertilization in secondary forest soils and adjacent oil palm plantation soils located in PT Kerry Sawit Indonesia, Central Kalimantan. A greenhouse experiment was conducted using both secondary forest and oil palm plantation soils to evaluate the impacts of different synthetic fertilizers on nitrogen-fixing bacteria and soybean agronomic performance. Total soil bacteria and diazotrophic bacteria, essential for nitrogen fixation, were analyzed through microbiological assays and qPCR focusing on the nifH gene. Our findings show that oil palm plantation soils had higher levels of nitrogen, phosphate, and nitrate, while secondary forest soils had a higher abundance of diazotrophic bacteria. Furthermore, excessive nitrogen fertilization was found to reduce microbial density, negatively impacting plant sustainability, highlighting the importance of customized fertilizer management. The study highlights the intricate connections between land-use practices and microbial populations, providing valuable insights for balancing agricultural productivity with ecological sustainability.
Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris Puspitasari, Esti; Rustam, Yepy Hardi; Satya, Andreas Adhi; Suwanto, Antonius; Wahyudi, Aris Tri; Astuti, Rika Indri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.310-319

Abstract

Bacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in Pichia pastoris and it was first performed on lipase from Geobacillus stearothermophilus T1.2. In this study, the T1.2RQ lipase gene from Geobacillus stearothermophilus T1.2 was expressed in Pichia pastoris GS115 through a strategy involving multiple copies of lipase, resulting in increased lipase activity. Three copies of the lipase gene in pPIC9K_T1.2RQ(3x) recombinant plasmid were integrated into the genome of Pichia pastoris GS115, and quantitative analysis using qPCR technique confirmed that the GS115 transformant strain contained six copies of T1.2RQ gene, indicating two integration events. Lipase activity measurement showed that the GS115/T1.2RQ(6x) strain exhibited a 111% increase compared to that containing a copy of the T1.2RQ gene. SDS-PAGE and Zymogram results showed a protein band with a size of 43kDa. Qualitative analysis in LA+TBN media of all strains containing the T1.2RQ gene showed clear zones. Lipase production in flask fermentation took at least 120 hours to produce the best lipase activity. Thus, strategies with multiple copy numbers of gene lipase have significantly increased the expression of the bacterial lipase gene in Pichia pastoris GS115.
Characterisation of ACE2-like Enzyme from Bacillus cereus sp. as an Alternative Treatment for COVID-19 Patients Maulida, Alya Sendra; Anggraeni, Novi Arie; Fibriani, Azzania
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.47-54

Abstract

SARS-CoV-2 infection can lead to downregulation of ACE2, raising pro-inflammatory Angiotensin II levels. Recombinant human ACE2 protein therapy can restore homeostasis but is costly. An alternative is an ACE2-like enzyme with similar effects. The previous research identified a carboxypeptidase protein from Bacillus cereus sp. (rBceCP) as a potential ACE2-like enzyme, but it has not been characterized well. This study characterizes, expresses, and tests the activity of the rBceCP). rBceCP structure and properties were predicted using Prabi and Expasy. The in vitro approach included protein expression optimization, hydrolysis tests, and inhibition tests. In silico analysis revealed the protein is a homodimer with 53.66% α-helix and a molecular weight of 58.99 kDa. The protein is stable, hydrophilic, and has an isoelectric point at pH 4.93, indicating it can be expressed using the E. coli system. Expression of rBceCP showed no significant differences across IPTG concentrations (p-value >0.05). The protein hydrolysis activity of rBceCP was similar to the control, though purified samples had lower activity than crude samples. Inhibition activity in crude and purified samples showed no significant differences (p-value >0.05) and was higher than the control. Thus, rBceCP has potential as an ACE2-like enzyme and a therapeutic candidate for COVID-19.
Bird Diversity and Existence at Pringtali Block: Implications for Conservation in Meru Betiri National Park, Indonesia Mohammad Siddiq, Arif; Sulistiyowati, Hari; Aprilia Palupi, Annisaa; Firmansyah, Puji
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.561-570

Abstract

Meru Betiri National Park (MBNP) is one of the conservation areas with an essential bird habitat in East Java. In the conservation monitoring and evaluation framework, we studied the diversity and existence of birds in the bird hotspot (Pringtali Block) as an implication for bird conservation in the MBNP. Bird observations were collected during January-February 2023 at the Pringtali Block using the point count method at six representative sites. The primary data recorded included bird species, abundance of each species, and frequency of encounters. Meanwhile, the secondary data were obtained from web queries covering bird conservation status and distribution through https://www.iucnredlist.org/. Data analysis was conducted in three stages: determining species composition, species diversity index (H'), and species existence value (Ef). The results recorded 70 bird species, consisting of 57 genera and 33 families, in the Pringtali Block of MBNP. The diversity index of birds was included in the high category (H' = 3.31). The average existence value (Ef) of the bird is 48.57 or x̄=3 s, which are included in the medium category. This reveals that birds in the Pringtali block have important structural and functional values in the MBNP ecosystem. This value also illustrates the importance of the presence of a bird species in the ecosystem and represents the uniqueness of the bird species. The birds with the highest Ef values were the Javan Hawk-eagle Nisaetus bartelsi (86.67) and White-rumped Woodpecker Meiglyptes tristis (86.67). Both species have low frequencies, are an endangered category, and are endemic to Java. Therefore, conservation efforts associated with MBNP have been successful.
Optimization of Protease Activity of Endophytic Bacteria EUA-136 and EUA-139 from Bruguiera gymnorrhiza Using Response Surface Methodology Agustien, Anthoni; Zovia, Miftahul; Dwitaviani, Rima; Marlida, Yetti
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.426-435

Abstract

Protease is a vital enzyme used in industries such as detergents, pharmaceuticals, and animal feed, with a growing demand in the enzyme market. Endophytic microorganisms can produce stable proteases with a rapid synthesis process. This study optimized conditions of temperature, pH, salinity, agitation, and nutrient sources for protease production by EUA-136 and EUA-139 bacterial isolates. The research used Response Surface Methodology (RSM) with a Central Composite Design (CCD) in Design Expert Software 13.1 to identify optimal conditions and the bacterial isolates. The optimum conditions for the EUA-136 bacterial isolate to produce protease were 3% inoculum at 30 ºC, pH 7, 28.5 ppt salinity, and 150 rpm agitation. For the EUA-139 bacterial isolate, the optimum conditions were a carbon source of 1% (v/v) maltose, a nitrogen source of 1% (v/v) KNO3, casein as the inducer, and an inoculum concentration of 7.5% (v/v). Molecular identification of isolates EUA-136 and EUA-139 revealed similarities to Bacillus cereus strain 3TC-3 and Bacillus paramycoides 3665, respectively.
Characterization and Enzymatic Assay of Cellulase-Producing Probiotic Bacteria Isolated from Traditional Fermented Bamboo of Bangladesh Marma, Mauching; Chakroborty, Koushik; Lee, Jong Min; Rahman, Zinia; Rafiquzzaman, S. M.
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.547-560

Abstract

Fermented bamboo (also known as Ngwopo) is a traditional food item among the indigenous population living in the south-east of Bangladesh. Though fermented foods harbour a significant number of probiotics and enzyme-producing bacteria, there is a lack of research on isolating beneficial bacteria from native fermented food products in Bangladesh. Thus, considering the extensive utilisation of cellulase enzymes and probiotics, our study was conducted with the objective of isolating and characterizing native cellulase-producing bacteria from fermented bamboo and performing crude enzyme activity assay. The sample was collected from Khagrachari hill tracts district of Bangladesh. Three isolated bacteria, namely FB-1, FB-2, and FB-3, screened based on the carboxymethyl cellulose medium, were tested for biochemical and probiotic properties, and the best isolate was genetically identified by molecular characterization through 16S rRNA gene sequencing and evolutionary analysis. All three gram-positive isolates were observed to be positive for starch hydrolysis and fermentation of sucrose, suggesting their ability to breakdown carbohydrates while testing negative for gelatinase, indole, and H2S test. The isolates demonstrated moderate bile salt and pH tolerance and low to medium autoaggregation. FB-2 had 75% susceptibility to the tested antibiotics, while other isolates were susceptible to 50% antibiotics. All of the bacteria were non-pathogenic, showing γ-haemolysis. FB-2 strain showed an enzyme production capacity of 1.56 units/ml. The best performing isolate was identified as Bacillus cereus. Therefore, the study discovered a native bacteria potentially suitable as probiotic and as a source of cellulase for industrial processes, highlighting the enriched indigenous cuisines of Bangladesh.
Sustaining Biodiversity and Ecological Roles in a Heritage Landscape: The Role of Coffee Agroforestry in Kluncing, Indonesia Kurnianto, Agung Sih; Dewi, Nilasari; Sulistyowati, Hari; Siddiq, Arif Mohammad; Ratnasari, Tri; Khowatini, Husnul; Yulianto, Roni; Firdaus, Anggun Sausan
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.459-471

Abstract

This study investigates the role of coffee agroforestry in sustaining biodiversity and ecosystem functions in Kluncing, Ijen, Indonesia. By quantifying bird, butterfly, and coffee insect diversity using the Shannon-Wiener index, it evaluates ecological dynamics and their implications for sustainable land management. Bird communities demonstrated the highest diversity (2.911), indicating ecosystem stability, while butterflies (2.481) and coffee insects (1.841) exhibited lower diversity, reflecting habitat and resource limitations. Trophic network modeling using NetworkX identified keystone species like Collocalia linchi (21.9% relative abundance) among birds and the Formicidae family (29.8% relative abundance) among coffee insects, emphasizing their critical roles in ecosystem balance. Canonical Correspondence Analysis (CCA) highlighted the influence of environmental factors on species distribution. Species such as Ariadne ariadne and Delias belisama were sensitive to humidity and temperature, while Collocalia linchi favored areas with higher light intensity. The findings underscore the importance of tailored management practices to address species-specific responses to microclimatic variations. Sustainable agroforestry management is vital for preserving biodiversity, maintaining ecosystem stability, and ensuring agricultural productivity. This study also highlights challenges posed by habitat degradation and climate change, emphasizing the need for adaptive strategies to safeguard this unique agroforestry landscape.
Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21 Taufik, Intan; Fanany, Rizal; Manjaswari, Agika; Pratama, Fenryco
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.155-163

Abstract

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (PCR) method. The growing demand for Bst DNA polymerase highlights the need for cost-effective in-house production, as a commercial option is economically challenging. For that purpose, this study aims to construct and optimize the expression of Bst DNA polymerase from Geobacillus stearothermophilus in Escherichia coli. The expression constructs pET16b.BstHF vector was constructed using Gibson Assembly and expressed in E. coli BL21 (DE3). Optimal expression was achieved with 1 mM IPTG, induction at OD600 0.8 and 6-hour induction time. The purified enzyme was achieved with a protein yield of 2,175 mg/L culture and demonstrated effective polymerase activity for LAMP.
The Invasive Plant Maeopsis eminii: A Key Dietary Resource for Primates in the Tropical Rainforests of West Java, Indonesia Noer, Mohamad Isnin; Lestari, Alvita Dwi; Ramadhini, Kiky; Prasetya, Priya Yuga; Sedayu, Agung; Komala, Ratna
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.528-536

Abstract

Animal-plant interactions are crucial for understanding the ecological dynamics and predicting ecosystem changes. This study explores the role of the invasive plant Maesopsis eminii in the Bodogol Nature Reserve, Gunung Gede National Park, West Java, Indonesia, focusing on its interaction with diurnal vertebrate species. Primates, including Javan gibbon and Javan langur, were identified as primary consumers of Maesopsis eminii, with Javan gibbon showing the highest consumption rates. Dietary analysis revealed that both primate species utilized M. eminii fruits and leaves, whereas Javan gibbons relied more on Maesopsis eminii than Javan langurs. Other vertebrates, such as squirrels and birds, are occasional visitors with minimal fruit consumption. This study highlights that Maesopsis eminii is a primary resource of Javan gibbon, Javan langur, and Javan surili. This nearly year-round fruiting plant is crucial for supporting primate populations, especially given its contribution to their diet. Considering the mutualistic interaction between Maesopsis eminii and its visitors, the Javan gibbon was the only species that probably dispersed seeds through endozoochory, making this primate species a crucial part of the dispersal of Maesopsis eminii in Gunung Gede National Park. Because invasive plants can threaten native flora and fauna, future studies must emphasize balancing the beneficial and detrimental effects.
Ecological Impact on Stingless Bees: Evaluating Pollen Load and Body Size Post-Introduction Suhri, Andi Gita Maulidyah Indraswari; Kahono, Sih; Riendriasari, Septiantina Dyah; Syahribulan; Salatnaya, Hearty; Rahmah, Mufti Hatur
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.278-286

Abstract

Changes in foraging behavior of stingless bees are caused by their introduction to new environments with varying biotic and abiotic factors. These bees struggle to adapt due to the shift from their original range. Biotic factors, particularly food source abundance, are crucial for their survival. Limited resources and reduced competition can decrease hive pollen, affecting larval nutrition and adult morphological growth. This study examines the physical effects of introducing stingless bees from Sulawesi to Jawa, Indonesia, using PCA to analyze 35 morphological traits and a haemocytometer to calculate pollen load. Morphometry was performed on ten separate colonies and ten different individual worker bees. Research findings indicate that the most significant size changes were observed in the forewing and hindwing lengths of worker bees at the introduction site. The smaller body size in the new environment indicates less available food in the nest. The lower pollen collection in the new environment compared to their natural habitat confirms this. The decline in pollen load is attributed to biotic factors like competition and food availability. When stingless bees become non-native, they face competition with existing species, including natives. Abiotic factors do not significantly influence the reduction in worker bees' foraging activity in the new environment. Changes in environmental factors significantly influenced the morphometric measurements of worker bees, following their introduction to lowland areas.

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