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HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 1,091 Documents
Genetic Characterization of Sumatran Mirah Chicken Based on Mitochondrial D-loop Region Sequence Silalahi, Parsaoran; Sitorus, Tunggul Ferry; Putra, Widya Pintaka Bayu
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.196-202

Abstract

Mirah chickens are Indonesian indigenous chickens that originate from Simalungun Regency, North Sumatera Province of Indonesia. The study aimed to determine the genetic characterization of Mirah chicken based on the mitochondrial D-loop region (838 bp). Twenty Mirah cocks from Simalungun Regency of Indonesia were used in the present study. The results showed that fourteen haplotypes were found in the studies of birds based on sixteen mutation sites. Therefore, these haplotype and nucleotide diversities in the partial D-loop region of the Mirah chicken were classified as high. The Median-joining tree revealed that the Mirah chickens were classified in a similar cluster with the Red jungle fowl (Gallus gallus). Ten haplotypes of birds were close to G. g. bankiva, and four haplotypes of birds were close to G. g. gallus. In conclusion, Mirah chickens had the genetic introgression from two sub-species of Red junglefowl, i.e., G.g. gallus and G.g. bankiva. Hence, the pure breeding program for Mirah chickens is important to conserve their genetic resources from extinction.
Analysis of Drought Stress Effect on Inpari Germination: Survival Method Rusdiana, Riza Yuli; Sa'diyah, Halimatus; Hadi, Alfian Futuhul
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.223-232

Abstract

Drought stress using mannitol can inhibit the germination of rice variety seeds. These studies typically produce time-to-event data and censored observation. Survival analysis techniques are valuable for accounting for these non-germination events, as they describe how germination probability changes over time based on the likelihood of seed development. Until now, there have not been survival studies regarding rice germination affected by drought stress in Indonesia. Thus, we investigated the germination probability of three rice varieties (Inpari 19, Inpari 32, and Inpari 49) under drought stress using survival analysis. The seeds were germinated in 0%, 2%, 4%, 6%, and 8% concentrations of mannitol and evaluated daily over 14 days. Our results demonstrated that higher mannitol concentrations significantly decreased the germination percentage and delayed germination time. The survival rates varied significantly between different mannitol concentrations, highlighting the adverse effects of drought stress. However, there was no significant difference in the probability of seed germination among the varieties treated with 2% mannitol. Among the varieties studied, Inpari 19 is more likely to be drought-resistant compared to Inpari 32 and Inpari 49. It is based on the highest germination percentage, shortest germination time, and highest probability of germination compared.
The Effectiveness of Insectary Plant that Attracts and Sustains Beneficial Arthropods to Control Spodoptera frugiperda J.E Smith (Lepidoptera: Noctuidae) in Maize Dewi, Vien Sartika; Sjam, Sylvia; Sulaeha, Sulaeha; Sulastri, Elsa
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.70-79

Abstract

Spodoptera frugiperda J.E. Smith, or fall armyworm (FAW), is a significant pest in maize plants and can reduce crop yields. Synthetic insecticides are still used to control S. frugiperda, but their use harms the environment and non-target organisms and can cause pests to become resistant. For this reason, alternative environmentally friendly technologies are needed, such as habitat management by planting insectary plants. This research aims to evaluate the potential of insectary plants to attract beneficial arthropods to suppress the S. frugiperda population and minimize the damage caused. This research activity was carried out using field research. The treatment in this research was the type of insectary plant used, and as a comparison, observations were made on land managed by farmers. The research results show that habitat management by planting insectary plants can increase the population of beneficial arthropods such as Coccinellidae, Formicidae, Miridae, Staphylinidae, and Araneidae. Increasing the population of beneficial arthropods can suppress the population of S. frugiperda so that its damage decreases and yields increase. These results show that planting insectary plants has the potential to prevent outbreaks of S. frugiperda, which can be combined with the application of other environmentally friendly technologies.
Scrutinize the Taxonomical Identity of Green Edible Russula from Sulawesi (Indonesia) Nurhayat, Oktan Dwi; Putra, Ivan Permana; Riffiani, Rini; Taridala, Sitti Aida Adha; Arif, Zulhan
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.436-444

Abstract

Russula Pers. (Basidiomycota) is recognized as one of the most widely distributed macrofungi globally. This genus includes several edible species with distinct color variations, such as R. cyanoxantha. However, in Indonesia, knowledge of this species remains limited as colourful mushrooms are often mistakenly considered poisonous. During a fungus expedition in Southeast Sulawesi, we collected a green edible wild mushroom consumed by locals. Our study aimed to verify the taxonomic classification of our specimens through morphological and molecular analysis. The fresh basidiomata were examined based on their macroscopic and microscopic characteristics. Molecular analysis using ITS 1/2 sequences was conducted to construct a phylogenetic tree. Our findings identified the green mushroom as R. cyanoxantha (Schaeff.) Fr. (Synonym: R. lilacina). Morphologically, our specimens can be distinguished from R. virescens by their smooth cap. They differ from R. aeruginea and R. heterophylla by having flexible, persistent gills. Additionally, our R. cyanoxantha specimen BO24636 can be separated from R. dinghuensis and R. subpallidirosea by their forked gills near the stalk. A BLAST comparison revealed a 96% similarity to R. lilacina from Thailand as the closest match. The phylogenetic tree (Maximum Likelihood) placed our specimens within the R. lilacina group (BS value 99%). Our research contributes to the taxonomic understanding of this edible wild green macrofungi in Indonesia, and future studies should explore its nutritional composition.
Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) Magandhi, Mahat; Sobir; Matra, Deden Derajat; Wahyu, Yudiwanti; Sudarmono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.132-139

Abstract

Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources.
Distribution and Utilization of Musa spp. by Nocte Tribe in Tirap District, Arunachal Pradesh, India Walung Songthing; Pangging, Govinda; Singh, S. Sureshkumar
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.89-105

Abstract

The present study emphasized the distribution and utilization of both wild and domesticated Musa spp. of the Nocte tribe from Tirap district, Arunachal Pradesh. An extensive field survey was conducted from July to October 2023. A total of 62 households were surveyed randomly with the help of a semi-constructed questionnaire and personal interview. About 69% of the informants were from the age group of 31-60 years. About 84% live in a nuclear family type. Maximum informants were male (74.19%) and were married (80.64%). Only 17.75% of the informants were illiterate and were farmers (46.77%). In this study, 13 wild Musa spp. were reported from Tirap district, and 4 Musa spp. were found to be cultivated by the tribe. Of these, 76.47% were wild, and the rest were domesticated. M. itinerens had a widespread distribution range and occurred in various habitats between 155 and 1,711 masl altitudes. The highest use percentage was found in the Edible use category with 33%, followed by Ceremonies and Rituals (19%), Commercial uses (12%), and both Other and Packing purposes (10% each). Among the plant parts, inflorescences had the highest usage with 30%, followed by leaf (24%), pseudo stem (21%), etc. Among all Musa spp., M. itinerens had the highest number of usages (14 uses), followed by M. nagensium (12 uses).
Assessment of Genetic Diversity Using Morphological and Molecular Characteristics of Indonesian Zoysiagrass Genotypes Rahayu; Fatimah; Mo, Yang Geun; Bae, Eun Ji
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.263-277

Abstract

Zoysiagrass is warm-season turfgrass thriving in tropical regions. Despite the adaptive nature, the existence of Indonesian zoysiagrass as well as morphological and genetic characteristics are not available. Therefore, this study aimed to explore Indonesian zoysiagrass from western and eastern parts of the country, as well as its morphological and molecular characteristics. Morphological characteristics was conducted to measure vegetative and reproductive characters while genotyping was performed using 15 simple sequence repeat markers. Morphological characteristics cluster three major groups, namely Group 1 corresponded to short, shorter, and fine leaves. Group 2 corresponded to tall, longer, and fine leaves, fewer seeds, and short spikelet, while Group 3 corresponded to tall, long, and wider leaves, more seeds, and longer spikelet. The results showed that the expected heterozygosity (He = 0.256) was lower than homozygosity (Ho = 0.341). The high level of discriminating capacity, polymorphism, and informativeness of SSR marker was observed (Effective Multiplex Ratio = 4.20, Marker Index = 2.394, and Resolving Power = 1.574). Additionally, population structure generated two subpopulations. Group 1 corresponded to Z. japonica from Sumatera Island and mixed province while Group 2 corresponded to Z. japonica from Central Java and Bali with Z. japonica and Z. matrella from mixed province. In conclusion, the exploration of morphological and genetic diversity from Indonesian zoysiagrass provided useful insight for conservation and future breeding improvement.
Study of Morphology and Growth of Alocasia spp. from Papua, Indonesia Daawia, Daawia; Kartika, Juang Gema; krisantini, krisantini; Rahayu, Megayani Sri; Asih, Ni Putu Sri; Matra, Deden D.; Suhartawan, Bambang
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.367-373

Abstract

Alocasia is a genus of perennial, flowering plants from Araceae, native to tropical and subtropical Asia and eastern Australia. The corms of some Alocasia species are edible, but many Alocasia species are cultivated as ornamentals. Despite the rich biodiversity of Alocasia, there have been limited studies on the morphology and anatomy of these genera. Our study aimed to describe the morphology and growth patterns of two Alocasia species found in Papua, one of the remote regions in Indonesia, A. brancifolia and A. lauterbachiana. The existence of A. lauterbachiana in Papua has never been reported before. The two Alocasia species can be distinguished by their distinct leaf shapes and colors. Alocasia brancifolia has pinnatifid leaves, while A. lauterbachiana has serrated leaves. The leaf of A. brancifolia is thin and predominantly green with faint spots, while the petioles exhibit stripes of brown and green, whereas A. lauterbachiana leaves are thicker and have darker green and reddish color. Due to their attractive leaf shape and colors, both species have the potential to be developed as ornamental potted plants. In terms of growth, A. lauterbachiana exhibited faster growth than A. brancifolia. However, A. brancifolia demonstrated a 50% increase in multiplication over the 16-week study period, while A. lauterbachiana primarily grew taller and larger. This information could prove valuable for future studies to optimize the growth and cultivation techniques of the two Alocasia species for commercial purposes, particularly ornamental foliage plants.
Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 Mat Jalil, Mohd Taufiq; Zakaria, Nurul Aili; Yahya, Mohd Fakharul Zaman Raja; Mohamad, Sharifah Aminah Syed; Ibrahim, Darah
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.445-458

Abstract

The biological activities of ethyl acetate extracts of Lasiodiplodia pseudotheobromae IBRL OS-64 were assessed in the present study. Antioxidant activity was measured using the DPPH scavenging assay, antibacterial activity via broth microdilution, and total phenolic content using the Folin-Ciocalteu method. In vivo toxicity was determined using a brine shrimp lethality assay. The fraction extract exhibited the highest antioxidant activity, with an EC50 of 441.6 µg/ml, surpassing the crude extract's. The EEELP showed a relatively low phenolic content of 13.273 µg GAE/mg extract. Regarding antibacterial activity, both crude and fraction extracts demonstrated MIC values ranging from 62.5 to 250 µg/ml against Gram-positive bacteria, while MBC values ranged from 125 to 500 µg/ml for both extracts. The MIC values for crude and fraction extracts against Gram-negative bacteria were 250-500 µg/ml, whilst the MBC values for both crude and fraction extracts were in the range of 500–2,000 µg/ml. The findings also revealed that Gram-positive bacteria were more susceptible to both extracts (crude and fraction) than Gram-negative bacteria. The crude extract exhibited a non-cytotoxic effect with an LC50 value of 2054.88 µg/ml for acute exposure and a low cytotoxic effect with an LC50 value of 199.69 µg/ml for chronic exposure. Meanwhile, the fraction extract demonstrated a non-cytotoxic effect for both acute and chronic with an LC50 value of 5744.14 µg/ml and 1035.94 µg/ml, respectively. Therefore, the present study suggests that the fraction extract could be an effective pharmaceutical agent and safe for drug development due to its low toxicity.
Artificial Neural Networks Model for Photosynthetic Rate Prediction of Leaf Vegetable Crops under Normal and Nutrient-Stressed in Greenhouse Suharto, Yohanes Bayu; Suhardiyanto, Herry; Susila, Anas Dinurroman; Supriyanto
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.300-309

Abstract

Photosynthesis is one of the essential processes in plant physiology that produces glucose and oxygen to support plant growth. Nutrient stress conditions will affect the photosynthetic rate in plants. The model predicting photosynthetic rates based on environmental conditions, nutrients, and plant types will be highly beneficial for farmers in tweaking these variables to maximize plant photosynthesis. This research focused on assessing the impact of nutrient stress on the photosynthetic rate in leaf vegetable crops and aimed to create a model using artificial neural networks (ANN) to predict photosynthetic rates under nutrient-stress conditions. Leaf vegetable crops were cultivated in a greenhouse using the NFT hydroponic system with eight nutrient conditions. This paper introduces an ANN model featuring nine input variables, ten hidden layers, and a single output. This model aims to elucidate the relationship between these inputs and the output parameter. The statistical analysis revealed a notable disparity in the CO2 assimilation rate among leaf vegetable crops subjected to nutrient stress treatment. The constructed ANN model demonstrated strong performance, achieving an R2 value of 0.9416, an RMSE of 1.5898 during training, and an R2 value of 0.9271 with an RMSE of 1.9649 in validation. A combination of statistical analysis and ANN modeling accurately explained the relationship and influence of input parameters, especially nutrient stress conditions, on the photosynthetic rate of leaf vegetable plants cultivated hydroponically in a greenhouse.

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