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HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 1,091 Documents
High-Resolution Microscopic Images of Mosquito Vectors (Diptera: Culicidae): Proper Identification of Culex and Aedes during Dengue Outbreak Situation in Bangladesh Yesmin, Farzana; Hasan, Md. Fahad; Hossain, Tahera; Akter, Shamme; Uddin, Md Nazim; Md. Hasanuzzaman
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.484-506

Abstract

High-resolution microscopic images of the 8th abdominal segment, comb scales, pectin, tuft of hairs, saddle, head antenna, thoracic hooks and other morphological characteristics of Culex quinquefasciatus, Aedes aegypti and Aedes albopictus have been provided for the first time in Bangladesh. Mosquitoes spread deadly diseases (dengue, chikungunya, Japanese encephalitis, yellow fever, malaria, filariasis) that kill millions of people every year including Bangladesh. During dengue and dengue outbreak situations, it is very much necessary to identify the dengue-occurring mosquito vectors. Proper identification of the mosquito vectors of Bangladesh is the key factor for public health protection and disease prevention. Larval movement patterns and habitat characterization were also observed. Results obtained in this study revealed that dengue virus can also be carried by other Aedes species rather than A. aegypti. Results were discussed with previous study dealing with Aedes mosquito samples of Bangladesh. The findings will be very useful for the proper identification of dengue mosquito vectors in Bangladesh and the knowledge assists in the accuracy of mosquito control activities as well as prevention of mosquito-borne diseases.
Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells Susanto, Hendra; Sholeh, Moch; Tri Endharti, Agustina; Nik Malek, Nik Ahmad Nizam; Amaliyah Firdaus, Sa’diyatul Rizqie; Yuda Handaya, Adeodatus; Taufiq, Ahmad
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.414-425

Abstract

Hepatocellular carcinoma (HCC) with high epidemiological report data. Pathogenesis in HCC also involves several signaling pathways. This study aims to evaluate the in vitro activity of Moringa seed NanoChitosan against Hep G2 liver cancer cells and Co-Treatment with Doxorubicin. Initially, nanoparticles were prepared by extracting Moringa seeds, formulating them into nano chitosan, and then characterizing the compounds and particle sizes. The IC50 dose was investigated using the MTT assay. Then, the IC50 dose was confirmed in more detail through immunofluorescence, betatrophin gene, several genes in the Wnt-βcatenin-CyclinD1 proliferation pathway, and the addition of the apoptotic effector Caspase-3 using RT-qPCR analysis. Each treatment used a single dose of NCH-Mosee and co-treatment or combination with 4 μg/ml doxorubicin. The IC50 dose was 994 μg/ml in single treatment and 649 μg/ml in combined treatment with Dox. Hep G2 showed a decrease in the expression level of each parameter measured with increasing single dose and combination treatment (p < 0.050). Histologically, cells shrank, betatrophin expression was inhibited, and luminescence was seen, which decreased with increasing dose. In conclusion, NCH-Mosee with dose-tracking toxicity combined with Dox can suppress the viability of Hep G2 cells.
In Silico and In Vitro Inhibitory Activity of Indonesian Herbal Compound Extracts against SARS-COV-2 Recombinant Papain-Like Protease Fatiningtyas, Fairuz Andini; Napitupulu, Riswanto; Malik, Amarila; Helianti, Is
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.356-366

Abstract

The SARS-CoV-2 papain-like protease (PLpro) is essential for viral replication and a promising target for drug discovery. This study explored the inhibitory potential of compounds from Indonesia herbals Butterfly pea flower (Clitoria ternatea L), Star fruit leaves (Averrhoa carambola L.), and Java plum leaves (Syzygium cumini (L.) Skeels) against PL pro through molecular docking and in vitro assays. The molecular docking method utilized the target protein PLpro (PDB ID: 7CMD), with the native ligand obtained from compounds identified in these plant extracts. The compounds were identified using the KNApSAcK database and analyzed for drug-likeness based on Lipinski's Rule of Five. The physicochemical characteristics affecting absorption, distribution, metabolism, excretion, and toxicity (ADMET) were determined using the pkCSM descriptor algorithm protocol. Validation was performed using the redocking method, achieving an RMSD score of 0.728 Å, which indicated validity (RMSD <2.0 Å). The results identified four ligands with the lowest binding affinities from these extracts: (-)-Epicatechin 3-O-gallate, folic acid, petunidin 3-glucoside, and ellagic acid, with binding scores of -8.6, -8.3, -7.1, and -7.1 kcal/mol, respectively. Prior to conducting the PLpro in vitro inhibition assay, a fluorescence-based inhibition assay was performed using Z-RLRGG-AMC as the substrate and GRL0617as the control inhibitor. All extracts were subjected to 70% ethanol maceration. The IC50 value of GRL0617 was 3.38 μM, while fluorescence tests showed that Java plum leaf extract exhibited the highest inhibition percentage at 66.10±3.22%. These findings indicate that all three plant extracts contain compounds capable of inhibiting PLpro activity.
Diversity of Indigenous Bacteria from Mangrove Sediments in the Waters of Ambon Bay, Maluku Tuasalamony, Mutia Musdalifah; Widiyanto, Tri; Rusmana, Iman
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.395-404

Abstract

This study aimed to analyze the diversity of indigenous bacteria by comparing culture and non-culture methods and to analyze the physicochemical effects on bacterial diversity in polluted and natural mangrove sediments. The environmental parameter values of mangrove sediments for bacterial growth can change owing to differences in adaptation and tolerance to fluctuations in physicochemical conditions. The number of colonies in natural and polluted areas using the culture method was 6.2 × 104 CFU/g and 5.5 × 104 CFU/g, respectively. A total of 33 isolates were identified, with 17 and 16 isolates from the natural and polluted areas, respectively. The most common isolates found in both areas were Acinetobacter haemolyticus strain FBC636 and Exiguobacterium acetylicum strain IAE17. Using the nanopore sequencing method, the total number of colonies in the natural and polluted areas was 69,761 and 58,412 colonies, respectively. A total of 12,954 bacterial species were identified, with 6,837 species in the natural area and 6,117 in the polluted area. The most common isolate found was Sulfurovum aggregans. Physicochemical conditions influenced the differences in bacterial diversity between the natural and polluted areas in the mangrove areas of Ambon Bay.
Diversity of Rhizospheric Bacterial Community from Kaolin Mining Site and Their Potential as Plant Growth Promoting Bacteria Armanisa, Khafifah; Rusmana, Iman; Astuti, Rika Indri
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.212-222

Abstract

Tailing from mining activities affects soil fertility resulting in poor soil conditions that are challenging for plants to grow. Plants can interact with rhizosphere bacteria to enhance their growth in harsh environments. Rhizospheric bacteria possess numerous mechanisms that promote plant growth and induced resistance to various abiotic stress. This study aims to determine the diversity of rhizobacteria and their potential as plant growth-promoting rhizobacteria (PGPR) agents. Bacterial communities from rhizosphere soil samples from kaolin mining sites in Perawas, Tanjung Pandan district, Belitung Regency, Bangka Belitung Island, Indonesia were analyzed using Next Generation Sequencing based on the V3-V4 region of the 16S rRNA gene, while culturable bacteria were isolated from samples and screened for PGP activity. The results showed that the rhizosphere bacterial community was mostly dominated by Pseudomonadota, Acidobacteria, and Verrumicrobiota. There were 15 bacteria isolated from the sample and RKB-5 bacterial isolate had the potential to be PGP agent. The RKB-5 bacterial isolate was identified as Burkholderia cenocepacia based on its 16S rRNA sequence. The bacterial isolate produced IAA, utilized ACC, dissolved phosphate up to 209,5 mg/L, and formed a high potassium solubilizer index value of 5.00. Therefore, the B. cenocepacia RKB-5 has potential application as the PGPR to support plants growth by obtaining nutrients in ex-mining lands with poor soil conditions.
Habitat Reduction and Population Structure of Endangered Asian Arowana (Scleropages formosus) Golden Red in Riau Sumatra, Indonesia Anthonius Purnama, Arief; Masunaga, Tsugiyuki
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.516-527

Abstract

This research was conducted in the arowana golden red Conservation Area (AgrCA) in Riau Province Rokan Hulu Regency, Sumatra, Indonesia from 2020 to 2023. The primary objective was to elucidate the population structure of arowana golden red in the midst of ongoing habitat degradation resulting from land use changes in AgrCA. Land use changes were analyzed by satellite imagery through the QGIS application. We collected population sampling data for arowana golden red by using 'fish net', 'fish trap', and 'fish scoop-net'. A comparison of satellite images taken in 2017 and 2021 revealed a reduction in swamp area by 664 ha (from 921 to 257 ha) and a decrease in swamp forest by 116 ha (from 264 to 148 ha). The conversion of swamp and swamp forests into oil palm plantations within the AgrCA has significantly diminished the natural habitat of the arowana golden red. Although there has been decreasing in swamp and swamp forests in AgrCA, the water quality in the AgrCA has remained suitable for the life of the arowana golden red. The population of arowana golden red was found to be 14-44 individuals year-1 in 2021-2023 with various sizes. Arowana golden red is most prevalent during the rainy season (July-December). Preserving the remaining swamp and swamp forests is paramount, representing a top priority. Immediate ex-situ conservation efforts are imperative, taking preemptive measures in case the arowana golden red can no longer inhabit its natural habitat.
Drought-Induced Morphophysiological and Metabolite Changes in Oil Palm Seedlings from Jambi, Indonesia Andesmora, Evan Vria; Hamim; Sulistijorini; Muttaqin, Mafrikhul; Triadiati
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.537-546

Abstract

Oil palm requires sufficient water for optimal growth. However, oil palm plantations in Indonesia face the challenge of global climate change, which causes increased temperatures and unpredictable rainy seasons. The research aimed to analyze oil palm accessions from Jambi that are tolerant to drought based on plant morphophysiological characteristics and metabolite profile. The experiment was conducted in the greenhouse using a factorial randomized block design. Five oil palm accessions were evaluated in this study, i.e., Muara Jambi (MJ), Tebo (TB), Tanjung Jabung Barat (TJB), Merangin (MR), and Simalungun (SM). Seedlings were planted in pots containing 20 kg of soil and acclimatized for 14 days before treatment. Drought treatments were carried out for two, four, and six weeks. Observations were made on growth, physiological, and metabolite changes at the end of each drought period. The study showed that the SM accession exhibited a higher photosynthetic rate and fresh and dry biomass than the other accessions. However, the content of proline and MDA increased significantly in oil palm throughout the drought periods. Metabolomic profiling revealed a significant increase in 13 metabolites under drought stress, i.e., citramalate, L-tyrosine, ferulate, and 3-4-dihydroxybenzoate were the four most prevalent metabolites in the leaf. The root produced more L-proline, aspartate, aconitate, kaempferol-3-o-pentoside, oxoadipate, citrate, L-phenylalanine, maltose, phenylacetic acid, and citramalate. These metabolites have roles in plant metabolic pathways such as valine, leucine, and isoleucine biosynthesis, phenylpropanoid biosynthesis, arginine and proline metabolism, citrate cycle (TCA cycle), lysine biosynthesis, phenylalanine metabolism, and starch and sucrose metabolism.
Chemical Components of Essential Oils from Woods of Cinnamomum burmanii (Ness) BL, Santalum album Linn., and Cryptocarya massoia (Oken) Kosterm and Their Application as Antibacterial Additives in Transparent Herbal Soaps Ariani, Sri Retno Dwi; Lestari, Tatik
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.632-641

Abstract

This study aimed to isolate and identify the chemical components of essential oils from woods of cinnamon (Cinnamomum burmanii (Ness) BL), sandalwood (Santalum album Linn.), and massoia (Cryptocarya massoia (Oken) Kosterm) and to determine their effect as additives for transparent herbal soap in terms of their antibacterial activity against Staphylococcus aureus ATTC 25923 and the soap quality. The results showed that cinnamon, sandalwood, and massoia essential oils contained, respectively, 14, 20, and 9 chemical components, all of which had been identified. The chemical components with the highest percentage contained in the essential oils of cinnamon, sandalwood, and massoia were cinnamaldehyde (91.71%), β-bisabolen (37.79%), and massoia lactone (77.56%), respectively. The soaps’ antibacterial activity against S. aureus ATTC 25923 increased from moderate (with an inhibitory power of 9.67 mm) to strong. The addition of 3% massoia essential oil got the highest inhibitory power of 16.47 mm (strong), followed by that of 3% cinnamon essential oil and 3% sandalwood essential oil, getting 15.37 mm and 12.19 mm, respectively, both of which were considered strong as well. The results of the organoleptic description test showed that each soap had a distinctive color and aroma depending on the type of essential oil added. The quality test results, including transparency, pH, foam stability, water content, and saponification number, indicated that the formulation had good quality.
Antibacterial Activities of Red Mangrove (Rhizophora stylosa Griff.) Leaf Extract against Klebsiella pneumoniae ATCC 700603 Kalasuba, Karina; Rahayuningsih, Sri Rejeki; Amalia, Eri; Syaputri, Yolani; Doni, Febri; Rustama, Mia Miranti
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.661-669

Abstract

Multidrug-resistant (MDR) Klebsiella pneumoniaeis a critical pathogen causing severe human diseases, including pneumonia. Combating the growing threat of MDRK. pneumoniae requires innovative approaches, such as exploring plant-derived antibacterial agents. Rhizophora stylosa Griff., a mangrove species with traditional medicinal uses, is recognized for its bioactive compounds with potential antibacterial properties. However, research on its bioactive constituents remains limited. This study investigated the antibacterial activity of R. stylosa leaf extracts prepared via maceration and liquid-liquid fractionation against K. pneumoniae ATCC 700603. Using agar-well diffusion and cell leakage assays, the water fraction demonstrated moderate inhibition of K. pneumoniae, producing an average inhibition zone of 8.24 mm and a minimum inhibitory concentration (MIC) of 400 mg/ml. UV-Vis spectrophotometry revealed that the water fraction disrupted protein and nucleic acid synthesis, evidenced by leakage of cellular materials at 260 and 280 nm. Additionally, scanning electron microscopy (SEM) images of K. pneumoniae cells treated with the water fraction showed pore formation and structural damage. These results emphasize the antibacterial potential of the water fraction of R. stylosa leaves against MDRK. pneumoniae. Further investigations are necessary to isolate and identify the specific bioactive compounds responsible for these effects. Moreover, comprehensive assessments of activity and toxicity are crucial to advance R. stylosa-based antibacterial agents as promising alternatives for addressing the challenge of MDR bacterial infections.
Oxidation Stress of UV C Light on Growth, Carotenoids and Chlorophyll-a Content of Chlorella vulgaris Sari, Luthfiana Aprilianita; Melva, Eva; Aldilameta, Sonia; Istiqomah, Nurul; Dewi, Nina Nurmalia; Arsad, Sulastri; Wan Omar, Wan Maznah
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.819-828

Abstract

C. vulgaris is photoautotrophic, which refers to microalgae that require light as an energy source. One of the light sources used to affect pigments in microalgae is UV light. The advantage of UV light is that it can change gene composition, which results in mutant genes in biopigment mutations and causes a higher number of activities compared to the natural ones, thereby increasing the growth, carotenoids, and chlorophyll-a of microalgae. The aim of this research is to determine the effect of oxidation stress of UV light on growth, carotenoids and chlorophyll-a content of Chlorella vulgaris. The treatments were given based on differences in the power of the UV-C light from, 8W, 15W and 30W. This study used the UV-Vis Spectrophotometer method was used to calculate growth, carotenoids and chlorophyll-a. Data were analysed using ANOVA test, Duncan's Multiple Range Test, and Principal Component Analysis (PCA). The results showed a significant effect of UV-C light. The best treatment was found in the administration of 30 W UV light, with the results of growth was 4.7746 × 106 cells/ml, content of carotenoids was 1.75 mg/ml and chlorophyll-a was 4.67 mg/ml of C. vulgaris. Biopigment can absorb radiation from UV-C light, which causes these microalgae to survive in environmental stress conditions. Absorbed light can affect the spectrum of pigments, thereby increasing growth, carotenoids, and chlorophyll-a.

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