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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 824 Documents
 69   70   71   72   73 
Kemampuan Rectovaginal Endoscopy (RVE) Mendeteksi Berahi dan Gambaran Kadar Estrogen Selama Siklus Berahi Kambing Dony Nurcahya; Widagdo Sri Nugroho; Agung Budiyanto; Budi Pramono
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.85245

Abstract

Deteksi estrus yang tepat pada kambing akan menentukan keberhasilan inseminasi buatan (IB). Rectovaginal endoscopy (RVE) merupakan alat deteksi estrus pada kambing yang dimodifikasi dengan perpaduan spekulum yang dilengkapai kamera terhubung dengan layar monitor. Alat RVE ini membantu peternak memastikan saat IB yang tepat. Penelitian ini bertujuan untuk mengetahui kemampuan Rectovaginal endoscopy (RVE) untuk mendeteksi gejala estrus pada  kambing-domba dan mengetahui gambaran estrogen selama siklus berahi kambing-domba. Metode penelitian dengan membandingkan deteksi estrus menggunakan kawin alami dengan pejantan (kelompok 1, kontrol) dengan konvensional/penglihatan mata langsung (Kelompok 2) dan RVE (kelompok 3). Masing-masing kelompok menggunakan 16 ekor kambing dengan ras jawa randu dengan Body Condition Score(BCS) 2,5-3 (kondisi bagus). Deteksi lendir dilakukan pada masing kelompok perlakuan dan diberi nilai (score). Setelah terdeteksi estrus, kelompok 2 dan 3 dilakukan inseminasi buatan (IB) sedangkan kelompok 1 kawin alami dengan pejantan. Data pengamatan estrus analisis secara deskriptif sedangkan data pengamatan lendir dianalisis menggunak Chie-Square. Koleksi serum darah untuk pemeriksaan kadar estrogen diambil pada hari ke-0, 3, 12, dan 15 siklus untuk mendapatkan gambaran kadar estrogen. Hasil deteksi estrus berdasarkan pengamatan lendir diketahui terdapat perbedaan signifikan diantara kelompok (P < 0,05). Pada hari ke-0 nilai profil hormon estrogen 36,14 pg/ml, tampilan gambar alat RVE menunjukkan lendir jernih bening dan terlihat banyak disaluran serviks sampai ke vulva. Hari ke-3 profil hormon estrogen 11,13 pg/ml, terlihat leleran pekat dan sedikit keruh dan hanya terlihat di sekitaran serviks. Hari ke-12 tampilan profil hormon estrogen 8,74 pg/ml terlihat lendir menjadi pekat keruh dan hanya terdapat di sekitar serviks. Hari ke-15 dengan tampilan hormon estrogen 15,82 pg/ml terlihat leleran bening dan hanya di sekitaran serviks. Disimpulkan bahwa penggunaan alat metode baru RVE memberikan pengaruh terhadap deteksi gejala estrus yaitu mampu mendeteksi lendir berdasarkan masing-masing fase siklus berahi.    
Efek Suplementasi Tepung Tulang Ikan Bandeng (Chanos chanos) dengan Metode Freeze Dry terhadap Performa Otot Ayam Bangkok (Gallus gallus domesticus) Astuti, Pudji; Yuneldi, Rizki Fitrawan; Divani, Adelia Lintang; Zahra, Nafisa Az; Rochmah, Jayanti Astridya; Airin, Claude Mona; Sarmin, Sarmin
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.85681

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung tulang ikan bandeng (Chanos chanos) yang dikeringbekukan menggunakan freeze-dry terhadap kadar testosteron pada otot pectoralis, bobot otot pectoralis, dan lingkar dada pada ayam bangkok (Gallus gallus domesticus). Penelitian ini menggunakan 10 ekor ayam bangkok jantan dengan umur 3-4 bulan yang terbagi menjadi dua kelompok perlakuan yaitu P0 (kontrol) dan P1 (tepung tulang ikan bandeng sebanyak 3,3 g/ekor/hari). Sebelum dilakukan penelitian dilakukan aklimatisasi selama 7 hari dan tepung tulang bandeng yang sudah dicampurkan dengan pakan diberikan setiap hari secara oral selama 35 hari. Pengukuran lingkar dada ayam bangkok dilakukan pada hari ke 7, 14, 21, 28, dan 35. Pada akhir penelitian ayam dinekropsi, kemudian dilakukan penimbangan otot pectoralis dan sampel homogenat otot pectoralis disentrifugasi dingin. Selanjutnya supernatan dikoleksi dan dianalisis menggunakan metode Enzyme-Linked Immunosorbent Assay (ELISA) untuk mengetahui kadar testosteron pada otot pectoralis. Data yang diperoleh kemudian dianalisis menggunakan independent sample T-Test dengan bantuan SPPS versi 26. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan (Sig. (2-tailed) < 0,05) pada kadar hormon testosteron otot pectoralis, bobot otot pectoralis dan lingkar dada ayam bangkok. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian tepung tulang ikan bandeng dengan metode freeze-dry dengan dosis 3,3 g/ekor/hari secara oral selama 35 hari dapat meningkatkan kadar testosteron pada otot pectoralis, bobot otot pectoralis dan lingkar dada pada ayam bangkok.
Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.) Gustina, Sri; Hasbi, Hasbi; Sonjaya, Herry; Baco, Sudirman; Qhatimah, Husnul; Saputra, Wandi; Mutmainna, Mutmainna; Kaiin, Ekayanti Mulyawati; Maulana, Tulus
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86154

Abstract

            The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P<0.01). Motility and acrosome integrity of frozen semen were not significantly difference (P>0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity.
Survei Infeksi Salmonella spp. pada Pasien Anjing dan Kucing di Klinik/Rumah Sakit Hewan Daerah Istimewa Yogyakarta Kajang, Elphan Augusta; Nugroho, Widagdo Sri; Haryanto, Aris
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86438

Abstract

Salmonellosis is an infectious disease that is zoonotic and includes food borne disease. Salmonella spp. can also be found in pets so that it can be a source of transmission of Salmonellosis to humans. The purpose of this study was to determine the infection level of Salmonella spp. in dogs and cats in clinics/animal hospitals in the Special Region of Yogyakarta. A total of 250 rectal swabs from 83 dogs and 167 cats were taken in this study. Isolation and identification of Salmonella spp. using Xylose Lysine Deoxycholate (XLD), Triple Sugar Iron Agar (TSIA), and Lysine Iron Agar (LIA) media. The isolates were confirmed by Polymerase Chain Reaction (PCR)using a primer Forward primer (5'- GCT AAG TAT GAC ATT CCG GT -3') and reverse (5'- CCA AAG ACT ATC TGC GGA AT -3') eith targeting the STM2773 (IroB) gene. Information on the patient's medical history was obtained based on the anamnesis and questionnaire to the animal owner. Data analysis was descriptive statistics. A total of 32 isolates of Salmomella spp. obtained through conventional methods and as many as 30 samples (12%) confirmed Salmonella spp. using PCR. A total of 11 (13.25%) of 83 dogs and 19 (11.27%) of 167 cats were identified as positive for Salmonella spp. The results of this study indicated that 12% of pet animals in the Special Region of Yogyakarta were infected with Salmonella spp.
Karagenin sebagai Model Inflamasi pada Kulit Punggung Mencit: Gambaran Makroskopik dan Histopatologis
Jurnal Sain Veteriner Vol 41, No 1 (2023): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86924

Abstract

This study aims to create a model of dorsal skin inflammation in mice using carrageenan with various concentrations. Forty-five female Swiss mice with the aged of 8 weeks were used in this study. These mice were divided into three groups of 15. Group I, II and III was given subcutaneous injection of 1%, 2% and 4% carrageenan respectively on the dorsum. Carrageenan was dissolved in 0.9% buffer saline with 0.1 mL volume injection. Prior to carrageenan injection, dorsal skin-fold thickness was measured using calipers and then was repeated every hour up to 6 hours. At the end of the sixth hour of measuring skin-fold thickness, animal was sacrificed, the dorsal skin was excised and then was fixed in 10% buffer formalin for histopathological examination. Data from skin-fold thickness measurement was analyzed using Two Ways Anova followed by Tukey HSD test. Results of this study demonstrate that there was a significant difference in skin-fold thickness between treatment groups (p<0.05). Sub cutaneous injection of 1%, 2% and 4% carrageenan resulted in increasing skin-fold thickness approximately 35%, 50% and 61% at the one-hour post carrageenan injection respectively (p<0.05). Skin-fold thickness gradually decreased at the second to the sixth hour after carrageenan injection. Histopathological examination shows mild and moderate dermal inflammation in the group given 1% and 2% carrageenan respectively. Severe dermal and panniculus inflammation was found in the group given 4% carrageenan. To conclude, carrageenan could be used to induce inflammation in back skin of mice.
Performa Estrus dan Hormon Estrogen Sapi Friesian Holstein Postpartus yang Diberi Urea Multinutrient Molasses Block (UMMB) dengan Perekat Tepung Tapioka Hasbi, Hasbi; Ramadan, Zyahrul; Utamy, Renny Fatmyah; Ako, Ambo; Masturi, Masturi; Gustina, Sri; Tasya, Tasya; Mufliha, Rara; Nurbina, Andi Fitri; Mutfaidah, Andi; Saputra, Rio; Mahayani, I Dewa Ayu; Mutmainna, Mutmainna; Rahman, Andi Arif; Sukri, Siti Annisa
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.87348

Abstract

The reproductive ability of Holstein Friesian (FH) cows greatly affects milk yield because, however, in the lactation period, dairy cows anestrus postpartus, which is the condition where does not appear in estrus postpartus. Anestrus postpartus to uterine involution as a consequence of nutritional deficiencies. Recovery from can be this condition can be conducted by additional feeding. One of the supplementary feeds is urea multinutrient molasses block (UMMB). UMMB consists of fillers and adhesives and cement is the most used in UMMB production as an adhesive. UMMB is an additional feed using cement dairy cows health problems. Therefore, the objective of this research was to determine the effect of cement substitution with tapioca meal as an adhesive on postpartus estrus of FH cows. A total of 9 lactating FH dairy cows were divided into 3 dietary groups. Each treatment had 3 cows as replicates. The dietary treatments consisted of UMMB with 100% cement adhesive (as D0); 50% cement and 50% tapioca (D1); and 0% cement and 100% tapioca meal (D2). The parameters were postpartus estrus, estrus speed, estrus duration, estrus intensity, and estrogen hormone levels. The results revealed that the treatment had a significant effect (P<0.05) on the duration of estrus, while it had no significant effect (P>0.05) neither on postpartus estrus, estrus speed, estrus intensity, or estrogen hormone levels. Therefore, it can be concluded that tapioca meals can be used as a substitute for cement as much as 50% provides as an adhesive in UMMB production for better estrus performance.
Splayed Leg in Birds: Diagnosis, Therapy, and Prevention Rief Ghulam Satriya Permana; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Anggitya Nareswari; Shafa Adea Puspitadesy
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.87656

Abstract

Splayed leg is a condition where the legs are stretched outwards or sideways from their normal position under the body which is often suffered by young birds. This disorder is usually left untreated, because it is considered as a permanent disability condition that cannot be treated without examining the cause. Some of the splayed legs in chicks can be improved by treating both their legs in the correct position. Early diagnosis of splayed leg and continued appropriate therapy is needed so that the condition of the foot can be restored to normal. Incorrect diagnosis and therapy will cause the bird's feet to become permanently disabled, so the bird will suffer for the rest of its life. The diagnosis and treatment of splayed leg cases have been developed with varying results. The purpose of this review is to evaluate the diagnosis, therapy, and prevention of the occurrence of splayed legs in birds, which can be used as a reference to improve bird welfare.  
Leukocyte Profile of Mice Infected with Plasmodium berghei and Treated with Aqueous Extract of Strychnos Ligustrina and Its Combination with Dihydroartemisinin Piperaquine Phosphate (DHP) Umi Cahyaningsih; Siti Sa&#039;diah; Wasrin Syafii; Rita Kartika Sari; Arifin Budiman Nugraha
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.88896

Abstract

Malaria is still one of the most highly pathogenic diseases, which remains a significant problem for public health. Natural compounds are alternative ways as an antimalarial compound that are applied for the artemisinin combination. The aim of this study was to determine the effect of a combination of S. ligustrina extract and DHP on the differential leukocytes percentages. This study used 100 mice which were divided into five groups. Group A and B were used for the healthy mice and infected-untreated groups, respectively. Group C used as a controlled drug, received 222 mg/kg BW of DHP. Group D received 300 mg/kg BW of aqueous extract of S. ligustrina. Group E received a combination between 111 mg/kg BW of DHP and 200 mg/kg BW of aqueous extract S. ligustrina. The findings demonstrated that DHP and S. ligustrina extract had an impact on neutrophils, lymphocytes, inhibition, and percentages of parasitemia. The findings revealed that treatment groups C, D, and E reduced the percentage of parasitemia. The percentage of neutrophils in treated groups was significantly higher than in group A (P<0.05). The percentage of lymphocytes was lower in the treated groups, while the percentages of eosinophils, monocytes, and basophils did not significantly different (P<0.05). This study found that group D and E had the same effect on the leucocyte profile as group C. Our findings suggest that the aqueous extract of S. ligustrina, as well as the combination treatment with DHP, have the potential to lead to the discovery of new antimalarial medicines.
Studi Perilaku Harian Rusa Timor (Cervus timorensis) di Lembaga Konservasi PT Taman Satwa Semarang Husna, Noora Lailatul; Kasiyati, Kasiyati; Djaelani, Muhammad Anwar; Setiawan, Hendrik Tri; Puri, Nur Liliana; Alrais, Aditya
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89306

Abstract

Adaptation of timor deer at the Conservation Institution of PT Taman Satwa Semarang is formed through a learning and memory process. The presence of visitors stimulates the deer to walk towards the side of the pen even though it is resting. Drop in feeding by keepers in the morning, afternoon, and evening causes the deer to become more active during the day. This study aims to analyze frequency and duration of the daily behavior of timor deer on weekdays with few visitors and on weekends with lots of visitors. Behaviors observed include moving, feeding, resting, grooming, aggressive, eliminative, and reproductive behavior. This research was conducted for 30 days. The object used in this study was a population of 18 adult timor deer. The method used is scan sampling, data recording of daily behavior which includes duration and frequency are carried out periodically at 10-minute intervals. The collected data includes daily behavioral, type of feed, as well as temperature and humidity. Data analysis was performed using t-test at a significance level of 5%. The results showed that visitor activity between weekdays and weekends had a significant effect (p˂0.05) on moving, feeding, resting, grooming, aggressive, and reproductive behavior. The conclusion of this study was the most timor deer perform resting and reproductive behaviors on weekdays, while moving, eating, and social behavior are mostly carried out on weekend.
Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Putri, Shafira Amalia; Maulina, Tifa Restyka; Dewi, Vira Kartika; Andityas, Morsid; Lestari, Fajar Budi; Hidayah, Nurulia; Ummami, Risa; Fauzi, Achmad
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89815

Abstract

Staphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan  coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi.

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